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The map label for this gene is lacA [C]

Identifier: 218709521

GI number: 218709521

Start: 1666782

End: 1667567

Strand: Direct

Name: lacA [C]

Synonym: VS_1530

Alternate gene names: 218709521

Gene position: 1666782-1667567 (Clockwise)

Preceding gene: 218709520

Following gene: 218709522

Centisome position: 50.52

GC content: 47.71

Gene sequence:

>786_bases
ATGACGTCAGTATCTATACATCAATTCAAACATTGGCTTAAGTTTCATCCTAATTCTCGAATTAGAAATGTGTTTTTCAT
TTTGAAAGACATCAGAAGCGCAGAGCTACCTACCCCTCAAATACTTAATCGTTGCCTCTATCAAGCGCACAAGCTAGTGG
TGAATTTGGTTTCTGGCTTGACGAGATTCTTCTACTGGACACCGGCTTTCAAAGGTCGTGTGGCTCAATGTGGCAAGCGC
CTGTATTTGTATGGTGGTCTGCCTTTCGTCAGCGGTCCGGTACAAATTACGATTGGCGACAACTGCCGTATCTCTGGCCA
CACGACATTCTCTGGTTGTACTCAGCCACTTGAAGGCTTGGAACACCCACTATTGTCGATTGGCAACAACGTCGACATCG
GTTGGCAGTCGACTATCGCTGTTGGAGGTAAAGTGGTTATCTCTGACAACGTACGTATCGCTGGCGGCGCGTTCCTATTT
GGATACTCTGGTCACCCGCTAGATGCAAAACGTCGAGCGCAAGGTGAAGGTGATGACCCTCAGCAAATCGGCGACATCAT
TCTTGAACCTGACGTTTGGTTAGGAACCAATGTAACTGTAAAAGGCGGCGTAACTATCGGCGAAGGTGCAGTGATAGCCG
CAGGAAGCGTGGTAACAAAGAATATCCCGGCCTTTGCAATCGCAGGTGGTAACCCTGCTCGAGTAGTCGGACAGATTAAA
TCCGTTGAAGTGACTGAATCCGATGTATTCGATTCATTAGAGACTCATGGAGGTGACCATGCGTGA

Upstream 100 bases:

>100_bases
AAACCTAAAGCCCACACTTTAACTCATTGAAAACATTATATTTATTAAATGGCACAGTATATGAAAGTACTTCTATATAA
CAAGATAGAGGAGTTTCATT

Downstream 100 bases:

>100_bases
TTTAATCGTATTTGGTGAAGACTTTGGCGGCCTACCGTCATCAACTCAACATTTAGTGCGTCACCTTGCTAAGCAACACA
AAGTCTTATGGGTCAACTCT

Product: acetyltransferase

Products: CoA; aacetyl-beta-D-galactoside [C]

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MTSVSIHQFKHWLKFHPNSRIRNVFFILKDIRSAELPTPQILNRCLYQAHKLVVNLVSGLTRFFYWTPAFKGRVAQCGKR
LYLYGGLPFVSGPVQITIGDNCRISGHTTFSGCTQPLEGLEHPLLSIGNNVDIGWQSTIAVGGKVVISDNVRIAGGAFLF
GYSGHPLDAKRRAQGEGDDPQQIGDIILEPDVWLGTNVTVKGGVTIGEGAVIAAGSVVTKNIPAFAIAGGNPARVVGQIK
SVEVTESDVFDSLETHGGDHA

Sequences:

>Translated_261_residues
MTSVSIHQFKHWLKFHPNSRIRNVFFILKDIRSAELPTPQILNRCLYQAHKLVVNLVSGLTRFFYWTPAFKGRVAQCGKR
LYLYGGLPFVSGPVQITIGDNCRISGHTTFSGCTQPLEGLEHPLLSIGNNVDIGWQSTIAVGGKVVISDNVRIAGGAFLF
GYSGHPLDAKRRAQGEGDDPQQIGDIILEPDVWLGTNVTVKGGVTIGEGAVIAAGSVVTKNIPAFAIAGGNPARVVGQIK
SVEVTESDVFDSLETHGGDHA
>Mature_260_residues
TSVSIHQFKHWLKFHPNSRIRNVFFILKDIRSAELPTPQILNRCLYQAHKLVVNLVSGLTRFFYWTPAFKGRVAQCGKRL
YLYGGLPFVSGPVQITIGDNCRISGHTTFSGCTQPLEGLEHPLLSIGNNVDIGWQSTIAVGGKVVISDNVRIAGGAFLFG
YSGHPLDAKRRAQGEGDDPQQIGDIILEPDVWLGTNVTVKGGVTIGEGAVIAAGSVVTKNIPAFAIAGGNPARVVGQIKS
VEVTESDVFDSLETHGGDHA

Specific function: May Assist Cellular Detoxification By Acetylating Nonmetabolizable Pyranosides, Thereby Preventing Their Reentry Into The Cell. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786537, Length=145, Percent_Identity=35.8620689655172, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1786664, Length=114, Percent_Identity=34.2105263157895, Blast_Score=66, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322243, Length=125, Percent_Identity=41.6, Blast_Score=82, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: NA

EC number: 2.3.1.18 [C]

Molecular weight: Translated: 28086; Mature: 27955

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSVSIHQFKHWLKFHPNSRIRNVFFILKDIRSAELPTPQILNRCLYQAHKLVVNLVSGL
CCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
TRFFYWTPAFKGRVAQCGKRLYLYGGLPFVSGPVQITIGDNCRISGHTTFSGCTQPLEGL
HHHEEECCCCCCHHHHCCCEEEEEECCCEECCCEEEEECCCEEECCCCCHHHHHHHHHHH
EHPLLSIGNNVDIGWQSTIAVGGKVVISDNVRIAGGAFLFGYSGHPLDAKRRAQGEGDDP
HHHHHHCCCCCCCCCCEEEEECCEEEEECCEEEECCEEEECCCCCCCCHHHHCCCCCCCH
QQIGDIILEPDVWLGTNVTVKGGVTIGEGAVIAAGSVVTKNIPAFAIAGGNPARVVGQIK
HHHCCEEECCCCEECCCEEEECCEEECCCCEEEECCEEECCCCEEEEECCCHHHEEEEEE
SVEVTESDVFDSLETHGGDHA
EEEECHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TSVSIHQFKHWLKFHPNSRIRNVFFILKDIRSAELPTPQILNRCLYQAHKLVVNLVSGL
CCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
TRFFYWTPAFKGRVAQCGKRLYLYGGLPFVSGPVQITIGDNCRISGHTTFSGCTQPLEGL
HHHEEECCCCCCHHHHCCCEEEEEECCCEECCCEEEEECCCEEECCCCCHHHHHHHHHHH
EHPLLSIGNNVDIGWQSTIAVGGKVVISDNVRIAGGAFLFGYSGHPLDAKRRAQGEGDDP
HHHHHHCCCCCCCCCCEEEEECCEEEEECCEEEECCEEEECCCCCCCCHHHHCCCCCCCH
QQIGDIILEPDVWLGTNVTVKGGVTIGEGAVIAAGSVVTKNIPAFAIAGGNPARVVGQIK
HHHCCEEECCCCEECCCEEEECCEEECCCCEEEECCEEECCCCEEEEECCCHHHEEEEEE
SVEVTESDVFDSLETHGGDHA
EEEECHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: MgSO4; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 1500 {isopropyl} 1200 {isopropyl} 770 {isopropyl} 360 {isopropyl} 0.18 {acetyl-CoA}} 0.049 {acetyl-CoA}} 0.045 {acetyl-CoA}} 0.04 {acetyl-CoA}} [C]

Substrates: Acetyl-CoA; a beta-D-galactoside [C]

Specific reaction: Acetyl-CoA + a beta-D-galactoside = CoA + aacetyl-beta-D-galactoside [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; Acetyl-CoA; Co2+; CoA; Cu2+; MgSO4; Mn2+; p-Chloromercuribenzoate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]