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The map label for this gene is luxO [H]

Identifier: 218709518

GI number: 218709518

Start: 1662455

End: 1663966

Strand: Direct

Name: luxO [H]

Synonym: VS_1527

Alternate gene names: 218709518

Gene position: 1662455-1663966 (Clockwise)

Preceding gene: 218709517

Following gene: 218709519

Centisome position: 50.39

GC content: 47.69

Gene sequence:

>1512_bases
ATGCGCCCTAAGGTATTGTTAGTTGAAGACTCCACCTCACTCGCCGTACTCTACAAGCAGTACGTAAAGGACGAGCCCTA
CGATATCTTCCACGTCGAAACCGGCGCGGAAGCTAAGACCTTCATTGAAAGGCACTCTCCACAGCTTGTTATTCTTGACC
TTAAGTTGCCCGACATGCCAGGGGAAGAAGTCTTAGATTGGATTAGTACGAACGAAATACCAACCGCCGTCATTATCGCG
ACGGCACATGGCTCAGTAAACATTGCTGTTGACCTTATCCAGCGCGGTGCGGAAGACTTCTTAGAGAAACCCATTCAAGC
CGATCGACTCAAGACTTCTGTTCGCTTACATCTAAAAAGAGCGAAACTCGAAAACCTTGTCGATAACATGCAAAGCAAGT
TCGATCGTGACCGCTTTCATAATTTCATAGGCTCTTGCCTTCCCATGCAAGCGGTTTACAAGATTATCGATTCGGTAGCA
CCAACCACCGCCAGCGTGTTTATTAACGGTGAAAGTGGTACGGGTAAAGAAGTGTGTGCTGAAGCGATCCACCAAGAGAG
CCAGCGTAACGGTAAGCCCTTCGTGGCTATTAACTGTGGTGCTATCCCTCGAGACCTAATGGAAAGTGAAATCTTCGGTC
ACGTTAAAGGTGCATTTACCGGTGCGACAACCGACCGTAAAGGTGCCGCAATGCAAGCGCATGGTGGCACCCTATTTCTC
GATGAGCTTTGTGAAATGGAACTGGAGATGCAGAAGAAGCTCCTACGATTTTTGCAAACTGGTACGTTTACCCCACTCGG
TGGCAACCGCGAGATTAAAGTGGATGTCAGAATTATCTGTGCGACCAACCGCGATCCACTGGTTGAGGTAGAAGAAGGAC
GCTTTAGAGAAGACCTTTACTATCGTGTTCACGTTGTGCCTATCGAGATGCCACCGCTTCGTGAACGAGGAAGTGACATT
GTCACCCTGTCTAATCACTTCTTGAAGCTGTACGCGAAGCAAGACAAGAAGAAGTTCAAATCAATAGACAAAGAAACGCA
GAATCTACTTAAACGTTATGCGTGGCCAGGCAACGTACGTCAGTTGCAAAACATCATCCGTAACATCGTGGTGTTGAACA
ATGAAACGAGCGTGACTAAAGACATGTTACCGCCTCCGATTAATAAAGTAGACGTAGCTACAGCCACGAAATCAGTGACA
CCTATTCGAGTAGCTGCGCCCCAAGCTGCTGTTGTGGAAGCACCAGAAACTAAGTTGCCACCTATTACACCGGAAGAGTT
GGAGCAATCCTCAGTTGTCCCCGCTAACAACGATCCGATGACGCCCGCATTTACAACAAGCGAAGGTGCTATTCGTCCAA
TGTGGCAAATAGAGCGCGAAGCTATTCAACATGCCATCAATCATTGCGATGGGAATGTGTTAAACGCTGCCGTGCTGTTA
GAACTTAGCCCTTCTACTGTTTATCGTAAGAAACAGGCTTGGGAATCGGAAGATGAGTACAATCAAGCCTAG

Upstream 100 bases:

>100_bases
GATTGCATGCGGTAGATAACAAATACACAAGACTCGTATTGATGGTTTCATATCACTATGTTATTGATTGGTTAGGATAA
AGTTTCAAAAGGAATACACA

Downstream 100 bases:

>100_bases
CCAAGGAGTACTCAACGTAAACCTTCACGAGATTTGGCTGTTGATCGACAGCCAAACTTTTGGAGGCATAGAAACGCATG
TTTTAGAGTTAGCACAAGGG

Product: putative LuxO repressor protein

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 503; Mature: 503

Protein sequence:

>503_residues
MRPKVLLVEDSTSLAVLYKQYVKDEPYDIFHVETGAEAKTFIERHSPQLVILDLKLPDMPGEEVLDWISTNEIPTAVIIA
TAHGSVNIAVDLIQRGAEDFLEKPIQADRLKTSVRLHLKRAKLENLVDNMQSKFDRDRFHNFIGSCLPMQAVYKIIDSVA
PTTASVFINGESGTGKEVCAEAIHQESQRNGKPFVAINCGAIPRDLMESEIFGHVKGAFTGATTDRKGAAMQAHGGTLFL
DELCEMELEMQKKLLRFLQTGTFTPLGGNREIKVDVRIICATNRDPLVEVEEGRFREDLYYRVHVVPIEMPPLRERGSDI
VTLSNHFLKLYAKQDKKKFKSIDKETQNLLKRYAWPGNVRQLQNIIRNIVVLNNETSVTKDMLPPPINKVDVATATKSVT
PIRVAAPQAAVVEAPETKLPPITPEELEQSSVVPANNDPMTPAFTTSEGAIRPMWQIEREAIQHAINHCDGNVLNAAVLL
ELSPSTVYRKKQAWESEDEYNQA

Sequences:

>Translated_503_residues
MRPKVLLVEDSTSLAVLYKQYVKDEPYDIFHVETGAEAKTFIERHSPQLVILDLKLPDMPGEEVLDWISTNEIPTAVIIA
TAHGSVNIAVDLIQRGAEDFLEKPIQADRLKTSVRLHLKRAKLENLVDNMQSKFDRDRFHNFIGSCLPMQAVYKIIDSVA
PTTASVFINGESGTGKEVCAEAIHQESQRNGKPFVAINCGAIPRDLMESEIFGHVKGAFTGATTDRKGAAMQAHGGTLFL
DELCEMELEMQKKLLRFLQTGTFTPLGGNREIKVDVRIICATNRDPLVEVEEGRFREDLYYRVHVVPIEMPPLRERGSDI
VTLSNHFLKLYAKQDKKKFKSIDKETQNLLKRYAWPGNVRQLQNIIRNIVVLNNETSVTKDMLPPPINKVDVATATKSVT
PIRVAAPQAAVVEAPETKLPPITPEELEQSSVVPANNDPMTPAFTTSEGAIRPMWQIEREAIQHAINHCDGNVLNAAVLL
ELSPSTVYRKKQAWESEDEYNQA
>Mature_503_residues
MRPKVLLVEDSTSLAVLYKQYVKDEPYDIFHVETGAEAKTFIERHSPQLVILDLKLPDMPGEEVLDWISTNEIPTAVIIA
TAHGSVNIAVDLIQRGAEDFLEKPIQADRLKTSVRLHLKRAKLENLVDNMQSKFDRDRFHNFIGSCLPMQAVYKIIDSVA
PTTASVFINGESGTGKEVCAEAIHQESQRNGKPFVAINCGAIPRDLMESEIFGHVKGAFTGATTDRKGAAMQAHGGTLFL
DELCEMELEMQKKLLRFLQTGTFTPLGGNREIKVDVRIICATNRDPLVEVEEGRFREDLYYRVHVVPIEMPPLRERGSDI
VTLSNHFLKLYAKQDKKKFKSIDKETQNLLKRYAWPGNVRQLQNIIRNIVVLNNETSVTKDMLPPPINKVDVATATKSVT
PIRVAAPQAAVVEAPETKLPPITPEELEQSSVVPANNDPMTPAFTTSEGAIRPMWQIEREAIQHAINHCDGNVLNAAVLL
ELSPSTVYRKKQAWESEDEYNQA

Specific function: Involved in the regulation of different processes depending on the cell density. Acts together with sigma-54 to repress, perhaps indirectly, some genes [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=400, Percent_Identity=40.25, Blast_Score=288, Evalue=5e-79,
Organism=Escherichia coli, GI1788550, Length=387, Percent_Identity=38.2428940568475, Blast_Score=285, Evalue=3e-78,
Organism=Escherichia coli, GI1788905, Length=395, Percent_Identity=39.746835443038, Blast_Score=275, Evalue=5e-75,
Organism=Escherichia coli, GI1790437, Length=383, Percent_Identity=38.9033942558747, Blast_Score=271, Evalue=5e-74,
Organism=Escherichia coli, GI1789233, Length=265, Percent_Identity=44.1509433962264, Blast_Score=235, Evalue=4e-63,
Organism=Escherichia coli, GI87082117, Length=247, Percent_Identity=47.7732793522267, Blast_Score=227, Evalue=2e-60,
Organism=Escherichia coli, GI87082152, Length=366, Percent_Identity=37.7049180327869, Blast_Score=221, Evalue=1e-58,
Organism=Escherichia coli, GI1789087, Length=376, Percent_Identity=37.7659574468085, Blast_Score=215, Evalue=4e-57,
Organism=Escherichia coli, GI1787583, Length=266, Percent_Identity=40.2255639097744, Blast_Score=202, Evalue=5e-53,
Organism=Escherichia coli, GI1786524, Length=286, Percent_Identity=39.5104895104895, Blast_Score=197, Evalue=9e-52,
Organism=Escherichia coli, GI87081872, Length=283, Percent_Identity=34.982332155477, Blast_Score=181, Evalue=7e-47,
Organism=Escherichia coli, GI1789828, Length=247, Percent_Identity=33.6032388663968, Blast_Score=138, Evalue=8e-34,
Organism=Escherichia coli, GI87081858, Length=265, Percent_Identity=31.6981132075472, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1786838, Length=134, Percent_Identity=31.3432835820896, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 56310; Mature: 56310

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPKVLLVEDSTSLAVLYKQYVKDEPYDIFHVETGAEAKTFIERHSPQLVILDLKLPDMP
CCCCEEEEECCCHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHCCCCEEEEEEECCCCC
GEEVLDWISTNEIPTAVIIATAHGSVNIAVDLIQRGAEDFLEKPIQADRLKTSVRLHLKR
HHHHHHHHCCCCCCEEEEEEECCCCEEEHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHH
AKLENLVDNMQSKFDRDRFHNFIGSCLPMQAVYKIIDSVAPTTASVFINGESGTGKEVCA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHH
EAIHQESQRNGKPFVAINCGAIPRDLMESEIFGHVKGAFTGATTDRKGAAMQAHGGTLFL
HHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCEEEH
DELCEMELEMQKKLLRFLQTGTFTPLGGNREIKVDVRIICATNRDPLVEVEEGRFREDLY
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEEECCCCCEEEECCCCCCCCEE
YRVHVVPIEMPPLRERGSDIVTLSNHFLKLYAKQDKKKFKSIDKETQNLLKRYAWPGNVR
EEEEEEEECCCCCHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
QLQNIIRNIVVLNNETSVTKDMLPPPINKVDVATATKSVTPIRVAAPQAAVVEAPETKLP
HHHHHHHHEEEECCCCCHHHHCCCCCCCCEEHHHHCCCCCEEEEECCCCEEEECCCCCCC
PITPEELEQSSVVPANNDPMTPAFTTSEGAIRPMWQIEREAIQHAINHCDGNVLNAAVLL
CCCHHHHHCCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEE
ELSPSTVYRKKQAWESEDEYNQA
EECCHHHHHHHHCCCCCHHCCCC
>Mature Secondary Structure
MRPKVLLVEDSTSLAVLYKQYVKDEPYDIFHVETGAEAKTFIERHSPQLVILDLKLPDMP
CCCCEEEEECCCHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHCCCCEEEEEEECCCCC
GEEVLDWISTNEIPTAVIIATAHGSVNIAVDLIQRGAEDFLEKPIQADRLKTSVRLHLKR
HHHHHHHHCCCCCCEEEEEEECCCCEEEHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHH
AKLENLVDNMQSKFDRDRFHNFIGSCLPMQAVYKIIDSVAPTTASVFINGESGTGKEVCA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHH
EAIHQESQRNGKPFVAINCGAIPRDLMESEIFGHVKGAFTGATTDRKGAAMQAHGGTLFL
HHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCEEEH
DELCEMELEMQKKLLRFLQTGTFTPLGGNREIKVDVRIICATNRDPLVEVEEGRFREDLY
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEEECCCCCEEEECCCCCCCCEE
YRVHVVPIEMPPLRERGSDIVTLSNHFLKLYAKQDKKKFKSIDKETQNLLKRYAWPGNVR
EEEEEEEECCCCCHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
QLQNIIRNIVVLNNETSVTKDMLPPPINKVDVATATKSVTPIRVAAPQAAVVEAPETKLP
HHHHHHHHEEEECCCCCHHHHCCCCCCCCEEHHHHCCCCCEEEEECCCCEEEECCCCCCC
PITPEELEQSSVVPANNDPMTPAFTTSEGAIRPMWQIEREAIQHAINHCDGNVLNAAVLL
CCCHHHHHCCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEE
ELSPSTVYRKKQAWESEDEYNQA
EECCHHHHHHHHCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301; 9748465 [H]