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The map label for this gene is ribE [H]

Identifier: 218709507

GI number: 218709507

Start: 1649857

End: 1650642

Strand: Reverse

Name: ribE [H]

Synonym: VS_1516

Alternate gene names: 218709507

Gene position: 1650642-1649857 (Counterclockwise)

Preceding gene: 218709509

Following gene: 218709503

Centisome position: 50.03

GC content: 43.64

Gene sequence:

>786_bases
ATGACCAGCAAACGCTGCCCGAAGGGTTCGGCTAAAAGCGTTTTACTCTTTGTTGAGGGAGATTTGCTTAGGATACTAGG
CTACTTCCCCCTCGCCGCGATTAAAACGCTTTTATCTCGAACGAAATTTAACCGCGATAGGTCAATACGCCCTAGAATGC
TAAATAGGAATCTTATGTTTACAGGTATCGTTCAAGGCATGGCAACACTGGTTGTTATAAATAAAAAAGAGTTGTTTCAG
ACTCATACCATTGAGCTAAGCGACGAAATGGTTGAGGGATTAGCGATTGGTGCCTCAGTAGCTCATAACGGTTGTTGCTT
AACGGTAACGGAAATTTCGGGCAATCAGATTGCTTTTGATTTAATGCAAGCCACATTGCGATTGACGAACTTAGGCCAAC
TGACTGTTGGTGATAAAGTGAATGTTGAGCGAGCAGCAAAATTTGGTGATGAAATTGGCGGGCATAGTATGTCTGGCCAC
ATTACGTTGATGGCGAACTTAGTGGATGTGATCAAGACTGAAAACAACCGCACGCTTTGGTTTGAGTTGCCACAGGAATC
AATGAAGTATGTATTGACCAAGGGTTATATTGGCGTTGATGGTTGTTCATTGACGATAGGTGAAGTGGAAGAGAACCGTT
TCTCTGTACACTTGATTCCTGAAACGCTAAATCGCACTTTGTTTGGCAGCCGCGAAATAGGCGACCAAGTAAATATTGAG
TTTGACCCTCAAACACAAGCGATTGTTGATACTGTTGAGCGCGTGCTAGCAAACCAGAAGTCGTAG

Upstream 100 bases:

>100_bases
TCTTTCGAGCTGATTTTTGCAGCGAGTTGCTGGGTGTTTATACAAGGCAGAGGCTTTGATGTGTAGCTAGCCTACATGAG
AAGCCGATAACGTAGTAAAA

Downstream 100 bases:

>100_bases
TAGCGGTTCATCAATAACACTTGCCCTAAAAGCGTTGACTAAGTAAAAATCGAAGATGTTAAAAAAGAGCACATTAAGTG
CTCTTTTTCTATTTTTGTGT

Product: riboflavin synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MTSKRCPKGSAKSVLLFVEGDLLRILGYFPLAAIKTLLSRTKFNRDRSIRPRMLNRNLMFTGIVQGMATLVVINKKELFQ
THTIELSDEMVEGLAIGASVAHNGCCLTVTEISGNQIAFDLMQATLRLTNLGQLTVGDKVNVERAAKFGDEIGGHSMSGH
ITLMANLVDVIKTENNRTLWFELPQESMKYVLTKGYIGVDGCSLTIGEVEENRFSVHLIPETLNRTLFGSREIGDQVNIE
FDPQTQAIVDTVERVLANQKS

Sequences:

>Translated_261_residues
MTSKRCPKGSAKSVLLFVEGDLLRILGYFPLAAIKTLLSRTKFNRDRSIRPRMLNRNLMFTGIVQGMATLVVINKKELFQ
THTIELSDEMVEGLAIGASVAHNGCCLTVTEISGNQIAFDLMQATLRLTNLGQLTVGDKVNVERAAKFGDEIGGHSMSGH
ITLMANLVDVIKTENNRTLWFELPQESMKYVLTKGYIGVDGCSLTIGEVEENRFSVHLIPETLNRTLFGSREIGDQVNIE
FDPQTQAIVDTVERVLANQKS
>Mature_260_residues
TSKRCPKGSAKSVLLFVEGDLLRILGYFPLAAIKTLLSRTKFNRDRSIRPRMLNRNLMFTGIVQGMATLVVINKKELFQT
HTIELSDEMVEGLAIGASVAHNGCCLTVTEISGNQIAFDLMQATLRLTNLGQLTVGDKVNVERAAKFGDEIGGHSMSGHI
TLMANLVDVIKTENNRTLWFELPQESMKYVLTKGYIGVDGCSLTIGEVEENRFSVHLIPETLNRTLFGSREIGDQVNIEF
DPQTQAIVDTVERVLANQKS

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyze

COG id: COG0307

COG function: function code H; Riboflavin synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lumazine-binding repeats [H]

Homologues:

Organism=Escherichia coli, GI1787952, Length=205, Percent_Identity=64.8780487804878, Blast_Score=276, Evalue=1e-75,
Organism=Saccharomyces cerevisiae, GI6319733, Length=214, Percent_Identity=33.1775700934579, Blast_Score=105, Evalue=9e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001783
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00677 Lum_binding [H]

EC number: =2.5.1.9 [H]

Molecular weight: Translated: 28903; Mature: 28772

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: PS00693 LUM_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSKRCPKGSAKSVLLFVEGDLLRILGYFPLAAIKTLLSRTKFNRDRSIRPRMLNRNLMF
CCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCHH
TGIVQGMATLVVINKKELFQTHTIELSDEMVEGLAIGASVAHNGCCLTVTEISGNQIAFD
HHHHHHHEEEEEECHHHHHHEEEEEECHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHH
LMQATLRLTNLGQLTVGDKVNVERAAKFGDEIGGHSMSGHITLMANLVDVIKTENNRTLW
HHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHCCCCCCEEE
FELPQESMKYVLTKGYIGVDGCSLTIGEVEENRFSVHLIPETLNRTLFGSREIGDQVNIE
EECCHHHHHHHHHCCCEEECCCEEEEECCCCCEEEEEEEHHHHCHHHCCCCCCCCEEEEE
FDPQTQAIVDTVERVLANQKS
ECCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TSKRCPKGSAKSVLLFVEGDLLRILGYFPLAAIKTLLSRTKFNRDRSIRPRMLNRNLMF
CCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCHH
TGIVQGMATLVVINKKELFQTHTIELSDEMVEGLAIGASVAHNGCCLTVTEISGNQIAFD
HHHHHHHEEEEEECHHHHHHEEEEEECHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHH
LMQATLRLTNLGQLTVGDKVNVERAAKFGDEIGGHSMSGHITLMANLVDVIKTENNRTLW
HHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHCCCCCCEEE
FELPQESMKYVLTKGYIGVDGCSLTIGEVEENRFSVHLIPETLNRTLFGSREIGDQVNIE
EECCHHHHHHHHHCCCEEECCCEEEEECCCCCEEEEEEEHHHHCHHHCCCCCCCCEEEEE
FDPQTQAIVDTVERVLANQKS
ECCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9022701; 9023191; 9097039; 9278503; 11377200 [H]