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The map label for this gene is yebU

Identifier: 218709402

GI number: 218709402

Start: 1542142

End: 1543563

Strand: Reverse

Name: yebU

Synonym: VS_1411

Alternate gene names: 218709402

Gene position: 1543563-1542142 (Counterclockwise)

Preceding gene: 218709403

Following gene: 218709400

Centisome position: 46.78

GC content: 47.19

Gene sequence:

>1422_bases
TTGCACGCTAACGTATATATTCCTGAAGAATTCCTAACGCACATCGAATCAATCATGCCTAGTCATCTAGATATGGCTTC
TTTTGTCGCGTCATGCCAAAAACCACTTCGTAAAAGTATTCGAGTCAACACATTGAAAATCAGTGTTGAGGACTTCTTAG
TACGAGCAAAAGAGAAAGGCTGGGAACTGGAACCTGTACCTTGGTGCGAAACGGGGTTTTGGATTACAGCTGATGAAAGT
GAAGCGCCACTAGGTAATACGGCAGAACACATGTCTGGCCTTTTCTATATCCAAGAAGCCAGTTCTATGATGCCACCGTC
GGCACTTTTCCAAGGTGAGGCAGACTACCAAGCCGTATTAGACACTGCGGCTGCACCCGGTTCTAAAACAACGCAAATCG
CGGCGCTAATGAATAATCGCGGCGTATTGGTTGCCAATGAATACGCAGCAAGCCGCGTGAAAGTTCTTCATGCCAACATC
GAACGTTGTGGCGTTCGTAATGCCGCACTCAGTAACTTCGATGGTCGAGTTTTCGGTGGGTGGTTACCAGAACAGTTTGA
TGCTGTGCTGCTAGACGCGCCCTGCTCTGGTGAAGGTACGATTCGCAAAGACGCTGATGCGATGAAGAACTGGACGTATC
AATCTGTAGTCGATATTGCTGACACTCAAAAAGATCTGATCGAAAGTGCTTTTCATGCTCTCAAACCTAACGGCGTGCTG
GTTTACTCAACCTGTACGTTAAGCACAGAAGAGAACCAACAAGTGTGTCATCACCTAAAAGAGACCTTTGGTGATGCGGT
TGAGTTCGAATCTCTTGAATCACTATTCGACAATGCTAAAGCAACCACGACAGAAGAAGGCTTTCTACACATTTTCCCAC
AAGTGTATGATTCAGAAGGTTTCTTCGTTGCGCGCATCCGTAAACTGGCGTCTGTGACACCACCAGAAGTGAAAAAACGT
CTGGGCAAATTCCCATTTGAAAAAGCGTCAAAGAAAGCTCAACAAGAAGTCGCTGAACAACTGCTGGGCGCACTCGATAT
CGAACTACCAAGTGATACTCAAGTTTGGATTCGTGACAAAGACGTTTGGCTATTCCCGGAAGCGCTAGAACCTATGATCG
GTGAGTTCCGTTTCTCTCGCATGGGTATCAAGATCGCTGAAACCCATAAAAAAGGTTACCGCTGGCAGCACCAAGTCGCG
ACAACGCTCGCAACAGGCAATGAAGCCAACATTGTAGACCTTAGCATTGAAGATGCTCGTGAATGGTTCATGGGACGCGA
TGTTCGCCCTGAAGGCTTGTCAGGCGAAGGTGAAGTACTAGTGAAGTACAATGGTGCAATCATCGGCCTTGGTAAGTGGG
TTGGCAACCGAGTGAAGAACGGTTTACCACGCGAACTCGTGCGCGATAAGAACCTGTTCTAA

Upstream 100 bases:

>100_bases
CATTCGGCTGCATTTTTGTTGTTTACTATAAATATGGTTTAGGACGGCCTCATTTTGCTTTAAACTCCCCCGCATTAATG
CAATAAATCGAGACACTCTT

Downstream 100 bases:

>100_bases
TTATGCTTAAAGCATGCGAATAAACAAAAAGCCCAAACAAAGTAAATTGTTTGGGCTTTTTCTATTCACATCAGATAACT
CGGTGCGATTAAAAACAATG

Product: rRNA (cytosine-C(5)-)-methyltransferase RsmF

Products: NA

Alternate protein names: 16S rRNA m5C1407 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmF

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MHANVYIPEEFLTHIESIMPSHLDMASFVASCQKPLRKSIRVNTLKISVEDFLVRAKEKGWELEPVPWCETGFWITADES
EAPLGNTAEHMSGLFYIQEASSMMPPSALFQGEADYQAVLDTAAAPGSKTTQIAALMNNRGVLVANEYAASRVKVLHANI
ERCGVRNAALSNFDGRVFGGWLPEQFDAVLLDAPCSGEGTIRKDADAMKNWTYQSVVDIADTQKDLIESAFHALKPNGVL
VYSTCTLSTEENQQVCHHLKETFGDAVEFESLESLFDNAKATTTEEGFLHIFPQVYDSEGFFVARIRKLASVTPPEVKKR
LGKFPFEKASKKAQQEVAEQLLGALDIELPSDTQVWIRDKDVWLFPEALEPMIGEFRFSRMGIKIAETHKKGYRWQHQVA
TTLATGNEANIVDLSIEDAREWFMGRDVRPEGLSGEGEVLVKYNGAIIGLGKWVGNRVKNGLPRELVRDKNLF

Sequences:

>Translated_473_residues
MHANVYIPEEFLTHIESIMPSHLDMASFVASCQKPLRKSIRVNTLKISVEDFLVRAKEKGWELEPVPWCETGFWITADES
EAPLGNTAEHMSGLFYIQEASSMMPPSALFQGEADYQAVLDTAAAPGSKTTQIAALMNNRGVLVANEYAASRVKVLHANI
ERCGVRNAALSNFDGRVFGGWLPEQFDAVLLDAPCSGEGTIRKDADAMKNWTYQSVVDIADTQKDLIESAFHALKPNGVL
VYSTCTLSTEENQQVCHHLKETFGDAVEFESLESLFDNAKATTTEEGFLHIFPQVYDSEGFFVARIRKLASVTPPEVKKR
LGKFPFEKASKKAQQEVAEQLLGALDIELPSDTQVWIRDKDVWLFPEALEPMIGEFRFSRMGIKIAETHKKGYRWQHQVA
TTLATGNEANIVDLSIEDAREWFMGRDVRPEGLSGEGEVLVKYNGAIIGLGKWVGNRVKNGLPRELVRDKNLF
>Mature_473_residues
MHANVYIPEEFLTHIESIMPSHLDMASFVASCQKPLRKSIRVNTLKISVEDFLVRAKEKGWELEPVPWCETGFWITADES
EAPLGNTAEHMSGLFYIQEASSMMPPSALFQGEADYQAVLDTAAAPGSKTTQIAALMNNRGVLVANEYAASRVKVLHANI
ERCGVRNAALSNFDGRVFGGWLPEQFDAVLLDAPCSGEGTIRKDADAMKNWTYQSVVDIADTQKDLIESAFHALKPNGVL
VYSTCTLSTEENQQVCHHLKETFGDAVEFESLESLFDNAKATTTEEGFLHIFPQVYDSEGFFVARIRKLASVTPPEVKKR
LGKFPFEKASKKAQQEVAEQLLGALDIELPSDTQVWIRDKDVWLFPEALEPMIGEFRFSRMGIKIAETHKKGYRWQHQVA
TTLATGNEANIVDLSIEDAREWFMGRDVRPEGLSGEGEVLVKYNGAIIGLGKWVGNRVKNGLPRELVRDKNLF

Specific function: Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family

Homologues:

Organism=Homo sapiens, GI76150625, Length=311, Percent_Identity=34.0836012861736, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI76150623, Length=311, Percent_Identity=34.0836012861736, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI11545785, Length=224, Percent_Identity=32.5892857142857, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI32698918, Length=164, Percent_Identity=36.5853658536585, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI301336155, Length=259, Percent_Identity=27.7992277992278, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI39995082, Length=197, Percent_Identity=31.4720812182741, Blast_Score=87, Evalue=5e-17,
Organism=Homo sapiens, GI270288816, Length=342, Percent_Identity=26.3157894736842, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI23199998, Length=302, Percent_Identity=26.8211920529801, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI270288818, Length=302, Percent_Identity=26.8211920529801, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI8922322, Length=302, Percent_Identity=26.8211920529801, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI40316918, Length=170, Percent_Identity=30.5882352941176, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI87081985, Length=471, Percent_Identity=56.687898089172, Blast_Score=559, Evalue=1e-160,
Organism=Escherichia coli, GI2367212, Length=231, Percent_Identity=29.8701298701299, Blast_Score=92, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17536757, Length=345, Percent_Identity=32.1739130434783, Blast_Score=165, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI25143471, Length=217, Percent_Identity=32.7188940092166, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI115534021, Length=230, Percent_Identity=30, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71998419, Length=227, Percent_Identity=30.8370044052863, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324268, Length=290, Percent_Identity=36.8965517241379, Blast_Score=162, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6319447, Length=196, Percent_Identity=33.6734693877551, Blast_Score=107, Evalue=6e-24,
Organism=Drosophila melanogaster, GI22024126, Length=326, Percent_Identity=30.6748466257669, Blast_Score=138, Evalue=6e-33,
Organism=Drosophila melanogaster, GI21355201, Length=282, Percent_Identity=27.3049645390071, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24668781, Length=148, Percent_Identity=37.1621621621622, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI21356513, Length=146, Percent_Identity=37.6712328767123, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19920494, Length=179, Percent_Identity=29.0502793296089, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMF_VIBSL (B7VNJ8)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002417023.1
- ProteinModelPortal:   B7VNJ8
- GeneID:   7160984
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_1411
- HOGENOM:   HBG726909
- OMA:   VANEYSA
- ProtClustDB:   PRK11933
- GO:   GO:0005737
- HAMAP:   MF_01579
- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR011023
- TIGRFAMs:   TIGR00446

Pfam domain/function: PF01189 Nol1_Nop2_Fmu

EC number: =2.1.1.178

Molecular weight: Translated: 52777; Mature: 52777

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: ACT_SITE 245-245 BINDING 147-147 BINDING 174-174 BINDING 192-192

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHANVYIPEEFLTHIESIMPSHLDMASFVASCQKPLRKSIRVNTLKISVEDFLVRAKEKG
CCCCEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCEEEEEEEEHHHHHHHHHHCC
WELEPVPWCETGFWITADESEAPLGNTAEHMSGLFYIQEASSMMPPSALFQGEADYQAVL
CCCCCCCCCCCCCEEEECCCCCCCCCCHHHHCCEEEEECHHHCCCCHHHHCCCCHHHHHH
DTAAAPGSKTTQIAALMNNRGVLVANEYAASRVKVLHANIERCGVRNAALSNFDGRVFGG
HHHCCCCCCHHEEHEECCCCCEEEECHHHHHHHHHEEHHHHHCCCCHHHHCCCCCCEECC
WLPEQFDAVLLDAPCSGEGTIRKDADAMKNWTYQSVVDIADTQKDLIESAFHALKPNGVL
CCCCCCCEEEEECCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEE
VYSTCTLSTEENQQVCHHLKETFGDAVEFESLESLFDNAKATTTEEGFLHIFPQVYDSEG
EEEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCEEEECCHHCCCCC
FFVARIRKLASVTPPEVKKRLGKFPFEKASKKAQQEVAEQLLGALDIELPSDTQVWIRDK
HHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEEECC
DVWLFPEALEPMIGEFRFSRMGIKIAETHKKGYRWQHQVATTLATGNEANIVDLSIEDAR
CEEECHHHHHHHHHHHHHHHCCEEEEEHHCCCCCHHHHHHHHHCCCCCCCEEEEEHHHHH
EWFMGRDVRPEGLSGEGEVLVKYNGAIIGLGKWVGNRVKNGLPRELVRDKNLF
HHHHCCCCCCCCCCCCCCEEEEECCEEEECHHHHHHHHHCCCCHHHHHCCCCC
>Mature Secondary Structure
MHANVYIPEEFLTHIESIMPSHLDMASFVASCQKPLRKSIRVNTLKISVEDFLVRAKEKG
CCCCEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCEEEEEEEEHHHHHHHHHHCC
WELEPVPWCETGFWITADESEAPLGNTAEHMSGLFYIQEASSMMPPSALFQGEADYQAVL
CCCCCCCCCCCCCEEEECCCCCCCCCCHHHHCCEEEEECHHHCCCCHHHHCCCCHHHHHH
DTAAAPGSKTTQIAALMNNRGVLVANEYAASRVKVLHANIERCGVRNAALSNFDGRVFGG
HHHCCCCCCHHEEHEECCCCCEEEECHHHHHHHHHEEHHHHHCCCCHHHHCCCCCCEECC
WLPEQFDAVLLDAPCSGEGTIRKDADAMKNWTYQSVVDIADTQKDLIESAFHALKPNGVL
CCCCCCCEEEEECCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEE
VYSTCTLSTEENQQVCHHLKETFGDAVEFESLESLFDNAKATTTEEGFLHIFPQVYDSEG
EEEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCEEEECCHHCCCCC
FFVARIRKLASVTPPEVKKRLGKFPFEKASKKAQQEVAEQLLGALDIELPSDTQVWIRDK
HHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEEECC
DVWLFPEALEPMIGEFRFSRMGIKIAETHKKGYRWQHQVATTLATGNEANIVDLSIEDAR
CEEECHHHHHHHHHHHHHHHCCEEEEEHHCCCCCHHHHHHHHHCCCCCCCEEEEEHHHHH
EWFMGRDVRPEGLSGEGEVLVKYNGAIIGLGKWVGNRVKNGLPRELVRDKNLF
HHHHCCCCCCCCCCCCCCEEEEECCEEEECHHHHHHHHHCCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA