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The map label for this gene is oprF [H]

Identifier: 218709383

GI number: 218709383

Start: 1517644

End: 1518258

Strand: Reverse

Name: oprF [H]

Synonym: VS_1392

Alternate gene names: 218709383

Gene position: 1518258-1517644 (Counterclockwise)

Preceding gene: 218709384

Following gene: 218709382

Centisome position: 46.02

GC content: 40.49

Gene sequence:

>615_bases
ATGAAGCTTCTCAAAACTATACTCCCACTATGCTTCGTGGTTGCTTCTGCCAACATCATGGCTGAAACAAATGATGAATT
CACTTATCGTGCTCTTCCTAGCACCACACAAAGTTATGATCTCGAAGATGATGATAATGATGGTGTTATCAACGCTCGCG
ATTTATGTATAGACACACAAATAGGCGCTGAAATAGATAATGATGGCTGTGGCTCGTATTTCGAGTCTAGCGAAGAAAAA
GATCTTCATATTTTATTTGCCAATGACTCTGCGGAAATTAAACCTGTGTTTCTGAGCCAAATTCGTCAAATGGCTGCGTT
TCTAAAGCGTTACGAAAACACAAGTATTCAATTGCAAGGTTATGCTAGTAAAGTAGGTAATGCTGAATACAATCTCAAGC
TCTCAGAAGAACGTGCGGTCAATGTAAGACGTGCGCTCATCAGCAATGGTATCCCGCCATCAAGAGTCTATACCGTAGGG
TACGGAGATGCAGGCTTCCTTGATAATGACACTGAAACTAATCATGCATTGAACAGAAAAGTCGTAGCCTCTGTTGTCGG
ATTTAAAGGCAACATCAAAGAAGAGTGGCATATATTTACTAAGATCGGTAAATAG

Upstream 100 bases:

>100_bases
TACAGTACGCAAAGTATAGAGTCATGAATGCTACAGGTAACCTATTAACAGGATTACGTGTAGAGACTCCACAAGAATGG
AATGAAAAGGTAGAATATTA

Downstream 100 bases:

>100_bases
ACTCTACCGCACTCAAACCATACTGAAACGTTTGGTAAGCGTTTCAGTATCCTTATAATTCCTTGATCGCCATATACCTA
CCCCTTGTATAATGTTAATC

Product: putative outer membrane protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 204; Mature: 204

Protein sequence:

>204_residues
MKLLKTILPLCFVVASANIMAETNDEFTYRALPSTTQSYDLEDDDNDGVINARDLCIDTQIGAEIDNDGCGSYFESSEEK
DLHILFANDSAEIKPVFLSQIRQMAAFLKRYENTSIQLQGYASKVGNAEYNLKLSEERAVNVRRALISNGIPPSRVYTVG
YGDAGFLDNDTETNHALNRKVVASVVGFKGNIKEEWHIFTKIGK

Sequences:

>Translated_204_residues
MKLLKTILPLCFVVASANIMAETNDEFTYRALPSTTQSYDLEDDDNDGVINARDLCIDTQIGAEIDNDGCGSYFESSEEK
DLHILFANDSAEIKPVFLSQIRQMAAFLKRYENTSIQLQGYASKVGNAEYNLKLSEERAVNVRRALISNGIPPSRVYTVG
YGDAGFLDNDTETNHALNRKVVASVVGFKGNIKEEWHIFTKIGK
>Mature_204_residues
MKLLKTILPLCFVVASANIMAETNDEFTYRALPSTTQSYDLEDDDNDGVINARDLCIDTQIGAEIDNDGCGSYFESSEEK
DLHILFANDSAEIKPVFLSQIRQMAAFLKRYENTSIQLQGYASKVGNAEYNLKLSEERAVNVRRALISNGIPPSRVYTVG
YGDAGFLDNDTETNHALNRKVVASVVGFKGNIKEEWHIFTKIGK

Specific function: Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium [H]

COG id: COG2885

COG function: function code M; Outer membrane protein and related peptidoglycan-associated (lipo)proteins

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OmpA-like domain [H]

Homologues:

Organism=Escherichia coli, GI48994946, Length=100, Percent_Identity=35, Blast_Score=64, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011250
- InterPro:   IPR006664
- InterPro:   IPR006690
- InterPro:   IPR006665
- InterPro:   IPR008722 [H]

Pfam domain/function: PF00691 OmpA; PF05736 OprF [H]

EC number: NA

Molecular weight: Translated: 22713; Mature: 22713

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS00018 EF_HAND_1 ; PS51123 OMPA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLKTILPLCFVVASANIMAETNDEFTYRALPSTTQSYDLEDDDNDGVINARDLCIDTQ
CCHHHHHHHHHHHHHCCCEEEECCCCEEEEECCCCCCCCCCCCCCCCCEEEHHHEEEECC
IGAEIDNDGCGSYFESSEEKDLHILFANDSAEIKPVFLSQIRQMAAFLKRYENTSIQLQG
CCCEECCCCCCCHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEE
YASKVGNAEYNLKLSEERAVNVRRALISNGIPPSRVYTVGYGDAGFLDNDTETNHALNRK
EHHHCCCCEEEEEECHHHHHHHHHHHHHCCCCCHHEEEEECCCCCCCCCCCCCHHHHHHH
VVASVVGFKGNIKEEWHIFTKIGK
HHHHHHCCCCCCCCCEEEEEECCC
>Mature Secondary Structure
MKLLKTILPLCFVVASANIMAETNDEFTYRALPSTTQSYDLEDDDNDGVINARDLCIDTQ
CCHHHHHHHHHHHHHCCCEEEECCCCEEEEECCCCCCCCCCCCCCCCCEEEHHHEEEECC
IGAEIDNDGCGSYFESSEEKDLHILFANDSAEIKPVFLSQIRQMAAFLKRYENTSIQLQG
CCCEECCCCCCCHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEE
YASKVGNAEYNLKLSEERAVNVRRALISNGIPPSRVYTVGYGDAGFLDNDTETNHALNRK
EHHHCCCCEEEEEECHHHHHHHHHHHHHCCCCCHHEEEEECCCCCCCCCCCCCHHHHHHH
VVASVVGFKGNIKEEWHIFTKIGK
HHHHHHCCCCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1898935 [H]