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The map label for this gene is metR [H]

Identifier: 218709137

GI number: 218709137

Start: 1211917

End: 1212828

Strand: Reverse

Name: metR [H]

Synonym: VS_1144

Alternate gene names: 218709137

Gene position: 1212828-1211917 (Counterclockwise)

Preceding gene: 218709139

Following gene: 218709134

Centisome position: 36.76

GC content: 46.27

Gene sequence:

>912_bases
ATGATAGAGCTTAAACACCTTCGGACATTGACCACCTTGAGAGACAGCGGCTCGCTAACCGCGACTGCAACTTCTCTTCA
TCTGACTCAGTCGGCACTTTCTCACCAGTTGAAAGACCTTGAGGCCCGTATTGGCGGTCAGCTCTTTTTGCGTAAAACAA
GGCCGGTTAAATTTACCTCGGAAGGCGAAATTTTGTTAAAACTGGCTGATGAGATACAGCCACAAATCGCCAAAGCTGAA
AACGAAATCGCAAGCCTAAAAGAGGATGTGAATGGCCGATTGCACATGGCGATCGAGTGTCACTCATGCTTCCAATGGCT
TATGCCCGCTTTGAAGGAATACCAAGTTGCTTGGCCAAGCGTGACCTTAGATTTCTCGTCAGGGTTTGGGTTCGAGCCTC
TACCGGCATTGATGGCTGGAGAACTCGATTTGGTGATCACCTCTGATATACAGCCCCGTTCTGAAGTTCACTACGAACCG
CTTTTCGATTTTGAGATGCGTTTGATCACCGCGATCAACTCTCCTTTAGCCGAAAAGCCGAGTATTGAACCGCAAGATCT
TAGTGATATTACGATGCTCTCTTATCCGGTTCAAAAGCAGCGTCTCGATGTAGTGAAACACTTTTTGCAACCTGCAGGCG
TTGAACCAAAGAAATGGAAACAGGCAGACAACACATTGATGTTAGTGCAAATGGTATCGGCAGGTTTAGGTGTTGCAGCC
TTACCTAATTGGGCAATCAGTGAGTTTTCAAGACAAGGGTTAATCGCCAGTAAACCATTGGGCAAAGGACTTTCAAGAAG
GTTGTTCGCAGCGGTAAGAAACTCAGAAAAAGACAAACGTTACCTACAAGCTTTCTTTAGCACGGCAAGACAGCAAAGTA
AGAGTCACCTAGATGGCATTGAGGTCGTTTAA

Upstream 100 bases:

>100_bases
TAAATCCATCTGGACATCTAGACGTTTACAAATGAAAGAAAACACCGTAGGGTGATTATTAAGAAATATTGAATTGGCTT
AGGGAATATGAGGAATACTC

Downstream 100 bases:

>100_bases
GAGACTCGTTGTTGGGTTAGTTTATGCCTGCGTTGAACCTGATAGATATGCGCCAGAAACAAAAAAGTCACAGATTATCT
GTGACTTTTTATTACCAATA

Product: HTH-type transcriptional regulator MetR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MIELKHLRTLTTLRDSGSLTATATSLHLTQSALSHQLKDLEARIGGQLFLRKTRPVKFTSEGEILLKLADEIQPQIAKAE
NEIASLKEDVNGRLHMAIECHSCFQWLMPALKEYQVAWPSVTLDFSSGFGFEPLPALMAGELDLVITSDIQPRSEVHYEP
LFDFEMRLITAINSPLAEKPSIEPQDLSDITMLSYPVQKQRLDVVKHFLQPAGVEPKKWKQADNTLMLVQMVSAGLGVAA
LPNWAISEFSRQGLIASKPLGKGLSRRLFAAVRNSEKDKRYLQAFFSTARQQSKSHLDGIEVV

Sequences:

>Translated_303_residues
MIELKHLRTLTTLRDSGSLTATATSLHLTQSALSHQLKDLEARIGGQLFLRKTRPVKFTSEGEILLKLADEIQPQIAKAE
NEIASLKEDVNGRLHMAIECHSCFQWLMPALKEYQVAWPSVTLDFSSGFGFEPLPALMAGELDLVITSDIQPRSEVHYEP
LFDFEMRLITAINSPLAEKPSIEPQDLSDITMLSYPVQKQRLDVVKHFLQPAGVEPKKWKQADNTLMLVQMVSAGLGVAA
LPNWAISEFSRQGLIASKPLGKGLSRRLFAAVRNSEKDKRYLQAFFSTARQQSKSHLDGIEVV
>Mature_303_residues
MIELKHLRTLTTLRDSGSLTATATSLHLTQSALSHQLKDLEARIGGQLFLRKTRPVKFTSEGEILLKLADEIQPQIAKAE
NEIASLKEDVNGRLHMAIECHSCFQWLMPALKEYQVAWPSVTLDFSSGFGFEPLPALMAGELDLVITSDIQPRSEVHYEP
LFDFEMRLITAINSPLAEKPSIEPQDLSDITMLSYPVQKQRLDVVKHFLQPAGVEPKKWKQADNTLMLVQMVSAGLGVAA
LPNWAISEFSRQGLIASKPLGKGLSRRLFAAVRNSEKDKRYLQAFFSTARQQSKSHLDGIEVV

Specific function: Control of the last step in methionine biosynthesis; metR is a positive activator of the metA, metE and metH genes. It is also a negative regulator of its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790262, Length=297, Percent_Identity=55.2188552188552, Blast_Score=343, Evalue=9e-96,
Organism=Escherichia coli, GI1788887, Length=265, Percent_Identity=25.2830188679245, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1790208, Length=245, Percent_Identity=26.1224489795918, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI157672245, Length=228, Percent_Identity=22.8070175438596, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI145693105, Length=279, Percent_Identity=24.3727598566308, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1786401, Length=265, Percent_Identity=25.6603773584906, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1790399, Length=304, Percent_Identity=26.3157894736842, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788297, Length=248, Percent_Identity=22.5806451612903, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787879, Length=249, Percent_Identity=25.3012048192771, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1789506, Length=169, Percent_Identity=26.0355029585799, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789173, Length=193, Percent_Identity=26.9430051813472, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1786448, Length=292, Percent_Identity=25.6849315068493, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33848; Mature: 33848

Theoretical pI: Translated: 7.28; Mature: 7.28

Prosite motif: PS50931 HTH_LYSR ; PS00300 SRP54

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIELKHLRTLTTLRDSGSLTATATSLHLTQSALSHQLKDLEARIGGQLFLRKTRPVKFTS
CCCHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEECC
EGEILLKLADEIQPQIAKAENEIASLKEDVNGRLHMAIECHSCFQWLMPALKEYQVAWPS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHEECCE
VTLDFSSGFGFEPLPALMAGELDLVITSDIQPRSEVHYEPLFDFEMRLITAINSPLAEKP
EEEECCCCCCCCCHHHHHCCCEEEEEECCCCCCHHCCCCCCHHHHHHHHHHHCCCCCCCC
SIEPQDLSDITMLSYPVQKQRLDVVKHFLQPAGVEPKKWKQADNTLMLVQMVSAGLGVAA
CCCCCCCCCHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHCCCCCHH
LPNWAISEFSRQGLIASKPLGKGLSRRLFAAVRNSEKDKRYLQAFFSTARQQSKSHLDGI
CCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCC
EVV
CCC
>Mature Secondary Structure
MIELKHLRTLTTLRDSGSLTATATSLHLTQSALSHQLKDLEARIGGQLFLRKTRPVKFTS
CCCHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEECC
EGEILLKLADEIQPQIAKAENEIASLKEDVNGRLHMAIECHSCFQWLMPALKEYQVAWPS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHEECCE
VTLDFSSGFGFEPLPALMAGELDLVITSDIQPRSEVHYEPLFDFEMRLITAINSPLAEKP
EEEECCCCCCCCCHHHHHCCCEEEEEECCCCCCHHCCCCCCHHHHHHHHHHHCCCCCCCC
SIEPQDLSDITMLSYPVQKQRLDVVKHFLQPAGVEPKKWKQADNTLMLVQMVSAGLGVAA
CCCCCCCCCHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHCCCCCHH
LPNWAISEFSRQGLIASKPLGKGLSRRLFAAVRNSEKDKRYLQAFFSTARQQSKSHLDGI
CCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCC
EVV
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]