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The map label for this gene is purR

Identifier: 218709082

GI number: 218709082

Start: 1146434

End: 1147438

Strand: Reverse

Name: purR

Synonym: VS_1088

Alternate gene names: 218709082

Gene position: 1147438-1146434 (Counterclockwise)

Preceding gene: 218709083

Following gene: 218709081

Centisome position: 34.78

GC content: 44.18

Gene sequence:

>1005_bases
ATGGCCACTATAAAAGATGTCGCACGCTTAGCCGGCGTATCAACAACAACCGTTTCTCACGTAATCAACAAAACTCGCTT
TGTTGCTGAAGCGACTCAAGAAAAAGTAAACAAAGCCGTAGACGAACTGAACTATGCGCCAAGTGCCGTTGCTCGTAGTT
TGAAGTGCAATACAACACGCACCATCGGTATGCTAGTGACTCAATCAACTAACCTATTCTTCTCTGAAGTTATCGATGGT
GTTGAGAGCTACTGCTACCGTCAAGGTTACACTTTGATCTTGTGCAATACGGGCGGTATCTACGAAAAGCAACGTGACTA
CATTCGAATGCTTGCAGAGAAACGTGTAGATGGCATCTTAGTTATGTGTTCCGACCTGACTGAAGAACTAAGAGAGATGT
TAGACCGTCACGCAGACATCCCTAAAGTTATCATGGACTGGGGCCCTGAAAGCTCTCAGGCTGATAAGATCATCGATAAC
TCTGAAGAAGGTGGCTACCTAGCAACCAAATATCTCATTGAGCGCGGTCACTCCAAGATTGCATGTTTAAGTGGCCACTT
AGACAAAGCAGCGTGTGTTGAACGCATCTCAGGTTACAAGCGCGCGCTCAATGAAGCGAAGATTACTGCCGATGAAAATA
TGATCATCGAAGGCAACTTTGAATGTGACACAGCTGTGATTGCTGCTGACCAAATCATTGAGATGGAAGAGCGCCCAACC
GCAGTATTCTGTTTTAACGACACGATGGCATTAGGACTGATGAGCCGATTACAAGAAAAAGGCATTCGTATTCCTGAAGA
TATCTCAGTGATCGGTTACGACAACATAGAATTGGCTGAATACTTCTCTCCACCGTTAACGACGGTTCACCAACCTAAGC
GCCGTGTTGGTAAAAATGCATTCGAAATACTACTAGAGCGTATAAAAGACAAAGATCACGAAAAACGCGTCTTCGAAATG
CACCCTGAAATTGTTGAGCGAAGTACAGTTAAAACGTTAAATTAA

Upstream 100 bases:

>100_bases
TAGTACCCAAAGAGCCCTACAAATTTAAGCTCTTAGTTAGGTAGCTTAACGGCTACTTCATTCCGTACTTTGGGAAAGTA
GCTTCTAATAGGATAAAAAC

Downstream 100 bases:

>100_bases
TTGATGTAATGAGTTTAGTTTAATCAAGCGCACCTTTAGGTGCGTTTTTTTCCTCAACAAACAAAGTGCACTTGAGTGCA
TAATTCAAAGATCATTAATT

Product: HTH-type transcriptional repressor PurR

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MATIKDVARLAGVSTTTVSHVINKTRFVAEATQEKVNKAVDELNYAPSAVARSLKCNTTRTIGMLVTQSTNLFFSEVIDG
VESYCYRQGYTLILCNTGGIYEKQRDYIRMLAEKRVDGILVMCSDLTEELREMLDRHADIPKVIMDWGPESSQADKIIDN
SEEGGYLATKYLIERGHSKIACLSGHLDKAACVERISGYKRALNEAKITADENMIIEGNFECDTAVIAADQIIEMEERPT
AVFCFNDTMALGLMSRLQEKGIRIPEDISVIGYDNIELAEYFSPPLTTVHQPKRRVGKNAFEILLERIKDKDHEKRVFEM
HPEIVERSTVKTLN

Sequences:

>Translated_334_residues
MATIKDVARLAGVSTTTVSHVINKTRFVAEATQEKVNKAVDELNYAPSAVARSLKCNTTRTIGMLVTQSTNLFFSEVIDG
VESYCYRQGYTLILCNTGGIYEKQRDYIRMLAEKRVDGILVMCSDLTEELREMLDRHADIPKVIMDWGPESSQADKIIDN
SEEGGYLATKYLIERGHSKIACLSGHLDKAACVERISGYKRALNEAKITADENMIIEGNFECDTAVIAADQIIEMEERPT
AVFCFNDTMALGLMSRLQEKGIRIPEDISVIGYDNIELAEYFSPPLTTVHQPKRRVGKNAFEILLERIKDKDHEKRVFEM
HPEIVERSTVKTLN
>Mature_333_residues
ATIKDVARLAGVSTTTVSHVINKTRFVAEATQEKVNKAVDELNYAPSAVARSLKCNTTRTIGMLVTQSTNLFFSEVIDGV
ESYCYRQGYTLILCNTGGIYEKQRDYIRMLAEKRVDGILVMCSDLTEELREMLDRHADIPKVIMDWGPESSQADKIIDNS
EEGGYLATKYLIERGHSKIACLSGHLDKAACVERISGYKRALNEAKITADENMIIEGNFECDTAVIAADQIIEMEERPTA
VFCFNDTMALGLMSRLQEKGIRIPEDISVIGYDNIELAEYFSPPLTTVHQPKRRVGKNAFEILLERIKDKDHEKRVFEMH
PEIVERSTVKTLN

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1787948, Length=331, Percent_Identity=51.6616314199396, Blast_Score=362, Evalue=1e-101,
Organism=Escherichia coli, GI1790194, Length=329, Percent_Identity=48.0243161094225, Blast_Score=339, Evalue=1e-94,
Organism=Escherichia coli, GI1790369, Length=334, Percent_Identity=33.5329341317365, Blast_Score=215, Evalue=3e-57,
Organism=Escherichia coli, GI1789068, Length=295, Percent_Identity=31.1864406779661, Blast_Score=167, Evalue=6e-43,
Organism=Escherichia coli, GI1789202, Length=301, Percent_Identity=34.5514950166113, Blast_Score=167, Evalue=7e-43,
Organism=Escherichia coli, GI1787580, Length=323, Percent_Identity=31.8885448916409, Blast_Score=165, Evalue=4e-42,
Organism=Escherichia coli, GI1788474, Length=335, Percent_Identity=32.5373134328358, Blast_Score=163, Evalue=1e-41,
Organism=Escherichia coli, GI1786540, Length=308, Percent_Identity=29.8701298701299, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI48994940, Length=322, Percent_Identity=28.2608695652174, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1790715, Length=334, Percent_Identity=26.3473053892216, Blast_Score=118, Evalue=5e-28,
Organism=Escherichia coli, GI1787906, Length=324, Percent_Identity=25, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1786268, Length=317, Percent_Identity=23.9747634069401, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1789456, Length=319, Percent_Identity=26.0188087774295, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1790689, Length=333, Percent_Identity=22.2222222222222, Blast_Score=85, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURR_VIBSL (B7VMG4)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002416703.1
- ProteinModelPortal:   B7VMG4
- GeneID:   7160680
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_1088
- HOGENOM:   HBG753640
- OMA:   DWGPESS
- ProtClustDB:   CLSK874518
- GO:   GO:0005622
- HAMAP:   MF_01277
- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761
- PRINTS:   PR00036
- SMART:   SM00354

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1; SSF47413 Lambda_like_DNA

EC number: NA

Molecular weight: Translated: 37613; Mature: 37481

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS00356 HTH_LACI_1; PS50932 HTH_LACI_2

Important sites: BINDING 73-73 BINDING 189-189 BINDING 220-220 BINDING 274-274

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIKDVARLAGVSTTTVSHVINKTRFVAEATQEKVNKAVDELNYAPSAVARSLKCNTTR
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCC
TIGMLVTQSTNLFFSEVIDGVESYCYRQGYTLILCNTGGIYEKQRDYIRMLAEKRVDGIL
EEEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEE
VMCSDLTEELREMLDRHADIPKVIMDWGPESSQADKIIDNSEEGGYLATKYLIERGHSKI
EEHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCHHHHCCCCCCCCCHHHHHHHHCCCCCE
ACLSGHLDKAACVERISGYKRALNEAKITADENMIIEGNFECDTAVIAADQIIEMEERPT
EEECCCCCHHHHHHHHHHHHHHHHHHEEECCCCEEEECCCCCCHHHHHHHHHHHHCCCCC
AVFCFNDTMALGLMSRLQEKGIRIPEDISVIGYDNIELAEYFSPPLTTVHQPKRRVGKNA
EEEEECCHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHCCCCCCHHCCHHHHHCHHH
FEILLERIKDKDHEKRVFEMHPEIVERSTVKTLN
HHHHHHHHHCCCHHHHHHHHCHHHHHHHHHHCCC
>Mature Secondary Structure 
ATIKDVARLAGVSTTTVSHVINKTRFVAEATQEKVNKAVDELNYAPSAVARSLKCNTTR
CCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCC
TIGMLVTQSTNLFFSEVIDGVESYCYRQGYTLILCNTGGIYEKQRDYIRMLAEKRVDGIL
EEEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEE
VMCSDLTEELREMLDRHADIPKVIMDWGPESSQADKIIDNSEEGGYLATKYLIERGHSKI
EEHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCHHHHCCCCCCCCCHHHHHHHHCCCCCE
ACLSGHLDKAACVERISGYKRALNEAKITADENMIIEGNFECDTAVIAADQIIEMEERPT
EEECCCCCHHHHHHHHHHHHHHHHHHEEECCCCEEEECCCCCCHHHHHHHHHHHHCCCCC
AVFCFNDTMALGLMSRLQEKGIRIPEDISVIGYDNIELAEYFSPPLTTVHQPKRRVGKNA
EEEEECCHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHCCCCCCHHCCHHHHHCHHH
FEILLERIKDKDHEKRVFEMHPEIVERSTVKTLN
HHHHHHHHHCCCHHHHHHHHCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA