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The map label for this gene is aat

Identifier: 218709071

GI number: 218709071

Start: 1133738

End: 1134448

Strand: Reverse

Name: aat

Synonym: VS_1077

Alternate gene names: 218709071

Gene position: 1134448-1133738 (Counterclockwise)

Preceding gene: 218709074

Following gene: 218709070

Centisome position: 34.38

GC content: 44.3

Gene sequence:

>711_bases
ATGACTATATACTTAACCGAACTTGATACTACTAGTATAGAGTTTCCTTCGCCCTTCGACGCGCTTGATGAACCCAACGG
TCTACTCGCGTTCGGTGGTGATTTGTCACCCATACGAATTCTGAACGCTTATAGCCAAGGTATATTTCCATGGTATGGCC
CAGGAGAGCCTATACTTTGGTGGAGCCCTGCACCGCGAGCGGTATTTAATCCTAAGACATTCGAACCATCAAAAAGTCTT
AAAAAATTTCAAAGAAAGCATAATTACCGTGTCAGCATCAACCAAGCGACGGACAGAGTAATTGGTTATTGCTCTTCACT
GAGACCCGAAGAGGAAACTTGGTTAAACAATGACATGCAAGCAGCCTACCGTGAACTCGCTTCATTAGGGTTCTGCCACT
CAGTTGAGGTTTGGCAAGGTGACGAGCTGATTGGCGGGCTTTATGGGTTACAAAGAGGCCAAGTCTTTTGCGGTGAATCG
ATGTTCAGCCTGAAGACGAATGCCTCTAAAATTGCGCTATGGTACTTTTGTGAGCACTTTACACAATTCGGTGGACAACT
CATTGATTGCCAAGTCATGAACCCACACTTGGAATCCTTGGGTGCTATCGAGGTTGATAGGGATGAATTCCTAAGTTCTC
TTCGATTACTCAAAGAAACGTCCGTTAACGATGCATGTTCTAAAAAACAATGGCTAAAGGAAATGCCTTAA

Upstream 100 bases:

>100_bases
TTTGCCAACATGGGCAAAACAAGTAAAATCCAAAGCAACTTCTTCATGGTAAAACTCCTAAAGACCTCTTGGTCAGTGAT
TAATCTGTAATGCGGTACTT

Downstream 100 bases:

>100_bases
TGAATCCAGATTTACAACAAATCAGAATTGGATTAACAGACAATCACGCTTGCAGTTACTTACCCCATCTCGAAGAAAGA
GTGGCAGTCACGCTTGATGA

Product: leucyl/phenylalanyl-tRNA--protein transferase

Products: NA

Alternate protein names: L/F-transferase; Leucyltransferase; Phenyalanyltransferase

Number of amino acids: Translated: 236; Mature: 235

Protein sequence:

>236_residues
MTIYLTELDTTSIEFPSPFDALDEPNGLLAFGGDLSPIRILNAYSQGIFPWYGPGEPILWWSPAPRAVFNPKTFEPSKSL
KKFQRKHNYRVSINQATDRVIGYCSSLRPEEETWLNNDMQAAYRELASLGFCHSVEVWQGDELIGGLYGLQRGQVFCGES
MFSLKTNASKIALWYFCEHFTQFGGQLIDCQVMNPHLESLGAIEVDRDEFLSSLRLLKETSVNDACSKKQWLKEMP

Sequences:

>Translated_236_residues
MTIYLTELDTTSIEFPSPFDALDEPNGLLAFGGDLSPIRILNAYSQGIFPWYGPGEPILWWSPAPRAVFNPKTFEPSKSL
KKFQRKHNYRVSINQATDRVIGYCSSLRPEEETWLNNDMQAAYRELASLGFCHSVEVWQGDELIGGLYGLQRGQVFCGES
MFSLKTNASKIALWYFCEHFTQFGGQLIDCQVMNPHLESLGAIEVDRDEFLSSLRLLKETSVNDACSKKQWLKEMP
>Mature_235_residues
TIYLTELDTTSIEFPSPFDALDEPNGLLAFGGDLSPIRILNAYSQGIFPWYGPGEPILWWSPAPRAVFNPKTFEPSKSLK
KFQRKHNYRVSINQATDRVIGYCSSLRPEEETWLNNDMQAAYRELASLGFCHSVEVWQGDELIGGLYGLQRGQVFCGESM
FSLKTNASKIALWYFCEHFTQFGGQLIDCQVMNPHLESLGAIEVDRDEFLSSLRLLKETSVNDACSKKQWLKEMP

Specific function: Functions in the N-end rule pathway of protein degradation where it conjugates Leu, Phe and, less efficiently, Met from aminoacyl-tRNAs to the N-termini of proteins containing an N-terminal arginine or lysine

COG id: COG2360

COG function: function code O; Leu/Phe-tRNA-protein transferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the L/F-transferase family

Homologues:

Organism=Escherichia coli, GI1787111, Length=216, Percent_Identity=54.6296296296296, Blast_Score=245, Evalue=2e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LFTR_VIBSL (B7VM36)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002416692.1
- GeneID:   7160669
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_1077
- HOGENOM:   HBG485363
- OMA:   GEPILWW
- ProtClustDB:   PRK00301
- GO:   GO:0005737
- HAMAP:   MF_00688
- InterPro:   IPR016181
- InterPro:   IPR004616
- TIGRFAMs:   TIGR00667

Pfam domain/function: PF03588 Leu_Phe_trans; SSF55729 Acyl_CoA_acyltransferase

EC number: =2.3.2.6

Molecular weight: Translated: 26822; Mature: 26690

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIYLTELDTTSIEFPSPFDALDEPNGLLAFGGDLSPIRILNAYSQGIFPWYGPGEPILW
CEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCCCEEE
WSPAPRAVFNPKTFEPSKSLKKFQRKHNYRVSINQATDRVIGYCSSLRPEEETWLNNDMQ
ECCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEECHHHHHHHHHHHCCCCCHHHHCCCCHH
AAYRELASLGFCHSVEVWQGDELIGGLYGLQRGQVFCGESMFSLKTNASKIALWYFCEHF
HHHHHHHHHCCCCEEEEECCCHHHHHHHHCCCCCEEECCCEEEEECCCCHHHHHHHHHHH
TQFGGQLIDCQVMNPHLESLGAIEVDRDEFLSSLRLLKETSVNDACSKKQWLKEMP
HHHCCEEEEEEEECCHHHHCCCEEECHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TIYLTELDTTSIEFPSPFDALDEPNGLLAFGGDLSPIRILNAYSQGIFPWYGPGEPILW
EEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCCCEEE
WSPAPRAVFNPKTFEPSKSLKKFQRKHNYRVSINQATDRVIGYCSSLRPEEETWLNNDMQ
ECCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEECHHHHHHHHHHHCCCCCHHHHCCCCHH
AAYRELASLGFCHSVEVWQGDELIGGLYGLQRGQVFCGESMFSLKTNASKIALWYFCEHF
HHHHHHHHHCCCCEEEEECCCHHHHHHHHCCCCCEEECCCEEEEECCCCHHHHHHHHHHH
TQFGGQLIDCQVMNPHLESLGAIEVDRDEFLSSLRLLKETSVNDACSKKQWLKEMP
HHHCCEEEEEEEECCHHHHCCCEEECHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA