Click here to switch to the map view.

The map label for this gene is xthA [H]

Identifier: 218709053

GI number: 218709053

Start: 1111465

End: 1112271

Strand: Reverse

Name: xthA [H]

Synonym: VS_1059

Alternate gene names: 218709053

Gene position: 1112271-1111465 (Counterclockwise)

Preceding gene: 218709054

Following gene: 218709047

Centisome position: 33.71

GC content: 45.23

Gene sequence:

>807_bases
ATGAAAGTAATCAGCTTCAACATCAATGGCCTTAGAGCCCGTCTTCATCAGCTTCAAGCTATTATCGATAAGCACCAACC
TGATGTAATTGGTCTACAAGAGATCAAAGTGCACGATGAAGCCTTCCCACTAGCCGATGTTGAAGCTATGGGTTACAAGG
TCTACTTCCACGGTCAAAAGGCTCACTACGGTGTGGCTATGCTTTGTAAGCAAGAGCCAATTTCGGTACAAAAAGGCTTT
CCTACAGACAATGAAGACCATCAAAAGCGTATGATCATGGCGACGTTTGAAGATGAACACGGTGAGAAGGTCACTGTGCT
AAATGGTTACTTCCCTCAAGGCGACAACATTAAGCATGAAACCAAATACCCGTACAAGCGCCAGTTCTACAAAGACTTAA
TGACGTACCTGAACGATTACCACAACAAAGATGAACAAGTGATCGTAATGGGCGACATTAATATCAGCCCTACCGATATT
GATATCGGCATTGGTGAACCTAATGCGAAACGTTGGTTGAAAACCGGTAAATGTTCTTTCCAACCAGAAGAACGCGAATG
GTTGAAAACATTGATGGATTGGGGTTTTGTGGACAGCTTCCGTTTACTACACCCTGAAGTGAACGATCAATACTCATGGT
TTGATTACCGCTCAAAAGGCTTCGTGGACAACCGCGGCCTGCGCATTGATGTGGTACTTGCGACTCAGAAGCTTGCTGAT
AAATGTACTGAAGCGGGCATCGACTACGAGCTACGCGGCATTGAAAAACCGTCAGACCACGCGCCGATTTGGTCGACGTT
TAAGTAG

Upstream 100 bases:

>100_bases
TATTAAGCATGCTTAAATACGATTAATCATGAAACCCACCAAATCAGCAAGCCCTTGAAAAGCAAGCAATCACAAGGGTT
TACGAGAGGTATAAAAACAA

Downstream 100 bases:

>100_bases
TTACTTTTAGGTAACAAAACTTAGCCATATAAAAAAGAGAGGAGTTAATATCTCCTCTCTTTTTTCTTCGTGACTCTATC
TGCCATATACCTAATTAAGC

Product: exonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MKVISFNINGLRARLHQLQAIIDKHQPDVIGLQEIKVHDEAFPLADVEAMGYKVYFHGQKAHYGVAMLCKQEPISVQKGF
PTDNEDHQKRMIMATFEDEHGEKVTVLNGYFPQGDNIKHETKYPYKRQFYKDLMTYLNDYHNKDEQVIVMGDINISPTDI
DIGIGEPNAKRWLKTGKCSFQPEEREWLKTLMDWGFVDSFRLLHPEVNDQYSWFDYRSKGFVDNRGLRIDVVLATQKLAD
KCTEAGIDYELRGIEKPSDHAPIWSTFK

Sequences:

>Translated_268_residues
MKVISFNINGLRARLHQLQAIIDKHQPDVIGLQEIKVHDEAFPLADVEAMGYKVYFHGQKAHYGVAMLCKQEPISVQKGF
PTDNEDHQKRMIMATFEDEHGEKVTVLNGYFPQGDNIKHETKYPYKRQFYKDLMTYLNDYHNKDEQVIVMGDINISPTDI
DIGIGEPNAKRWLKTGKCSFQPEEREWLKTLMDWGFVDSFRLLHPEVNDQYSWFDYRSKGFVDNRGLRIDVVLATQKLAD
KCTEAGIDYELRGIEKPSDHAPIWSTFK
>Mature_268_residues
MKVISFNINGLRARLHQLQAIIDKHQPDVIGLQEIKVHDEAFPLADVEAMGYKVYFHGQKAHYGVAMLCKQEPISVQKGF
PTDNEDHQKRMIMATFEDEHGEKVTVLNGYFPQGDNIKHETKYPYKRQFYKDLMTYLNDYHNKDEQVIVMGDINISPTDI
DIGIGEPNAKRWLKTGKCSFQPEEREWLKTLMDWGFVDSFRLLHPEVNDQYSWFDYRSKGFVDNRGLRIDVVLATQKLAD
KCTEAGIDYELRGIEKPSDHAPIWSTFK

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=269, Percent_Identity=24.1635687732342, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI18375503, Length=269, Percent_Identity=24.1635687732342, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI18375501, Length=269, Percent_Identity=24.1635687732342, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1788046, Length=268, Percent_Identity=63.4328358208955, Blast_Score=378, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI71989536, Length=268, Percent_Identity=26.1194029850746, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI221330655, Length=270, Percent_Identity=30.7407407407407, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI17136678, Length=270, Percent_Identity=30.7407407407407, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 31160; Mature: 31160

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVISFNINGLRARLHQLQAIIDKHQPDVIGLQEIKVHDEAFPLADVEAMGYKVYFHGQK
CEEEEECCHHHHHHHHHHHHHHHCCCCCEECCEEEEECCCCCCCCCHHHCCEEEEEECCC
AHYGVAMLCKQEPISVQKGFPTDNEDHQKRMIMATFEDEHGEKVTVLNGYFPQGDNIKHE
CCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEECCCCCCCCCCCC
TKYPYKRQFYKDLMTYLNDYHNKDEQVIVMGDINISPTDIDIGIGEPNAKRWLKTGKCSF
CCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEECCCCCHHHHHHCCCCCC
QPEEREWLKTLMDWGFVDSFRLLHPEVNDQYSWFDYRSKGFVDNRGLRIDVVLATQKLAD
CCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCEEECCCCCCCCCCCCEEEEEEECHHHHH
KCTEAGIDYELRGIEKPSDHAPIWSTFK
HHHHCCCCEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKVISFNINGLRARLHQLQAIIDKHQPDVIGLQEIKVHDEAFPLADVEAMGYKVYFHGQK
CEEEEECCHHHHHHHHHHHHHHHCCCCCEECCEEEEECCCCCCCCCHHHCCEEEEEECCC
AHYGVAMLCKQEPISVQKGFPTDNEDHQKRMIMATFEDEHGEKVTVLNGYFPQGDNIKHE
CCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEECCCCCCCCCCCC
TKYPYKRQFYKDLMTYLNDYHNKDEQVIVMGDINISPTDIDIGIGEPNAKRWLKTGKCSF
CCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEECCCCCHHHHHHCCCCCC
QPEEREWLKTLMDWGFVDSFRLLHPEVNDQYSWFDYRSKGFVDNRGLRIDVVLATQKLAD
CCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCEEECCCCCCCCCCCCEEEEEEECHHHHH
KCTEAGIDYELRGIEKPSDHAPIWSTFK
HHHHCCCCEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]