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The map label for this gene is holB [H]

Identifier: 218709024

GI number: 218709024

Start: 1070386

End: 1071351

Strand: Direct

Name: holB [H]

Synonym: VS_1030

Alternate gene names: 218709024

Gene position: 1070386-1071351 (Clockwise)

Preceding gene: 218709023

Following gene: 218709025

Centisome position: 32.44

GC content: 45.86

Gene sequence:

>966_bases
ATGGCTGAAGTTTATTCTTGGCTCACACCGGTATGGAGTGAGTGGAAAAAAAGTCTCGATGCAGAACGTTTTCCTAACTC
TGTGATCGTTAATGCGCCGGAAGGGCTTGGTGTGGAAGCGCTGGTTAGCCAACTTACCGATGCATTGATGTGTGTTAATT
ACGAAAGTGAAGCGTGCGGATTTTGCCACAGTTGTGAACTGATGAAGTCGGGGAGTCACCCAGATTTCCATGTGATTGAA
CCTGAGAAAGAAGGTAAATCGATTACCGTTGACCAAGTGCGTGCGAGTAACCGCTGGGCTCAACAATCGTCCCAGTTAGG
TGGCCTGCGTGTCATCTTGCTGAATCCGGCTGAAGCGATGAACGAATCGGCATCTAATGCGCTGTTGAAAACGCTGGAAG
AACCTGCGAGTAACTGTATTTTCATTCTTTCGACGCGCAACAGTAATCGCTTAATGCCAACTATTCTTAGCCGTTGCCAG
CAATTTAATGTGGTGTGCCCACAATTGGATGCAGGATCGGCATGGTTAAATGGAGAAGTTGGTAAAACGGTTCCTCAGTA
CATACTGACATTGAACGACAACGCACCTCTGAAAGCGAAAGCAATGTTTGATCAAGGTGGCGTCGAAGCTTCTACCAAAG
CACTAGATGGCTTTGTGGATGTGATAAAAGGCACTCAACCCGACATGCTGAAGTTCTCTACAGAGCTTAGCAAAGACCCT
TTGATTCAACTTAGCTGGCTTTGGCATTTATTGTCAGATGTACAAAAGCTGCATTTTGGTTTAGCTAATCACGCTATCAT
TCCGAGTGCGAAAGCACTGTGTGAGGTGATGTCTTATCAAAGTGCTTATGCAGCGTCGAATAAACTGCTGATATTGATTG
AGCAGTTAAAGCAACACCCTGGGCTCAATACGGAGCTACTCATAATGAATTGGCTGATAGCCACTTGCGAGGAAACATGT
TCGTAG

Upstream 100 bases:

>100_bases
CGATTCTGTACTCGTGATTAATGCGCAGCAAGAAATTGAGCAAGTAGCCGCTGATATTAAAGTCGCGCTCAGTGCTTGGC
TCAATAAACAGTAGGTAGTC

Downstream 100 bases:

>100_bases
ATTCCCATTGTCATTTAGACAAACTGGATTACCAAAATTTACACACCAGCGTAGAAGACGTGGTTAATAAAGCGAAAGCA
GCCAACGTTGACCAACTGCT

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MAEVYSWLTPVWSEWKKSLDAERFPNSVIVNAPEGLGVEALVSQLTDALMCVNYESEACGFCHSCELMKSGSHPDFHVIE
PEKEGKSITVDQVRASNRWAQQSSQLGGLRVILLNPAEAMNESASNALLKTLEEPASNCIFILSTRNSNRLMPTILSRCQ
QFNVVCPQLDAGSAWLNGEVGKTVPQYILTLNDNAPLKAKAMFDQGGVEASTKALDGFVDVIKGTQPDMLKFSTELSKDP
LIQLSWLWHLLSDVQKLHFGLANHAIIPSAKALCEVMSYQSAYAASNKLLILIEQLKQHPGLNTELLIMNWLIATCEETC
S

Sequences:

>Translated_321_residues
MAEVYSWLTPVWSEWKKSLDAERFPNSVIVNAPEGLGVEALVSQLTDALMCVNYESEACGFCHSCELMKSGSHPDFHVIE
PEKEGKSITVDQVRASNRWAQQSSQLGGLRVILLNPAEAMNESASNALLKTLEEPASNCIFILSTRNSNRLMPTILSRCQ
QFNVVCPQLDAGSAWLNGEVGKTVPQYILTLNDNAPLKAKAMFDQGGVEASTKALDGFVDVIKGTQPDMLKFSTELSKDP
LIQLSWLWHLLSDVQKLHFGLANHAIIPSAKALCEVMSYQSAYAASNKLLILIEQLKQHPGLNTELLIMNWLIATCEETC
S
>Mature_320_residues
AEVYSWLTPVWSEWKKSLDAERFPNSVIVNAPEGLGVEALVSQLTDALMCVNYESEACGFCHSCELMKSGSHPDFHVIEP
EKEGKSITVDQVRASNRWAQQSSQLGGLRVILLNPAEAMNESASNALLKTLEEPASNCIFILSTRNSNRLMPTILSRCQQ
FNVVCPQLDAGSAWLNGEVGKTVPQYILTLNDNAPLKAKAMFDQGGVEASTKALDGFVDVIKGTQPDMLKFSTELSKDPL
IQLSWLWHLLSDVQKLHFGLANHAIIPSAKALCEVMSYQSAYAASNKLLILIEQLKQHPGLNTELLIMNWLIATCEETCS

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787341, Length=315, Percent_Identity=30.7936507936508, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI1786676, Length=143, Percent_Identity=29.3706293706294, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008921
- InterPro:   IPR004622
- InterPro:   IPR015199 [H]

Pfam domain/function: PF09115 DNApol3-delta_C [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 35368; Mature: 35237

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEVYSWLTPVWSEWKKSLDAERFPNSVIVNAPEGLGVEALVSQLTDALMCVNYESEACG
CCCHHHHHHHHHHHHHHHCCHHHCCCCEEEECCCCCCHHHHHHHHHHHHEEECCCCHHCC
FCHSCELMKSGSHPDFHVIEPEKEGKSITVDQVRASNRWAQQSSQLGGLRVILLNPAEAM
CHHHHHHHHCCCCCCEEEECCCCCCCEEEHHHHHHHHHHHHHHHCCCCEEEEEECCHHHH
NESASNALLKTLEEPASNCIFILSTRNSNRLMPTILSRCQQFNVVCPQLDAGSAWLNGEV
HHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCEECCCC
GKTVPQYILTLNDNAPLKAKAMFDQGGVEASTKALDGFVDVIKGTQPDMLKFSTELSKDP
CCCCCCEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCC
LIQLSWLWHLLSDVQKLHFGLANHAIIPSAKALCEVMSYQSAYAASNKLLILIEQLKQHP
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEHHHHHCC
GLNTELLIMNWLIATCEETCS
CCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AEVYSWLTPVWSEWKKSLDAERFPNSVIVNAPEGLGVEALVSQLTDALMCVNYESEACG
CCHHHHHHHHHHHHHHHCCHHHCCCCEEEECCCCCCHHHHHHHHHHHHEEECCCCHHCC
FCHSCELMKSGSHPDFHVIEPEKEGKSITVDQVRASNRWAQQSSQLGGLRVILLNPAEAM
CHHHHHHHHCCCCCCEEEECCCCCCCEEEHHHHHHHHHHHHHHHCCCCEEEEEECCHHHH
NESASNALLKTLEEPASNCIFILSTRNSNRLMPTILSRCQQFNVVCPQLDAGSAWLNGEV
HHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCEECCCC
GKTVPQYILTLNDNAPLKAKAMFDQGGVEASTKALDGFVDVIKGTQPDMLKFSTELSKDP
CCCCCCEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCC
LIQLSWLWHLLSDVQKLHFGLANHAIIPSAKALCEVMSYQSAYAASNKLLILIEQLKQHP
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEHHHHHCC
GLNTELLIMNWLIATCEETCS
CCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]