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The map label for this gene is luxO [H]

Identifier: 218708976

GI number: 218708976

Start: 1015312

End: 1016658

Strand: Direct

Name: luxO [H]

Synonym: VS_0982

Alternate gene names: 218708976

Gene position: 1015312-1016658 (Clockwise)

Preceding gene: 218708975

Following gene: 218708977

Centisome position: 30.77

GC content: 45.14

Gene sequence:

>1347_bases
ATGGTAGAAGATACCGCGTCGGTAGCAGCGTTATATCGATCGTATCTTACACCGCTCGAAATTGATATTAACATTGTTGG
TACTGGCCGCGATGCAATCGAGAGTTTGAACCATCGAATCCCAGACCTCATTTTATTAGATCTACGCCTGCCCGATATGA
CGGGTATGGATGTACTGTTTGCTGTAAAACAAAAATATCCGGAAGTTCCCGTTATCTTCATGACTGCTCACGGCTCTATT
GATACCGCAGTAGAAGCCATGCGACATGGTTCCCAAGATTTCCTTATCAAGCCGTGTGAAGCCGACCGACTTCGTATTAC
GGTGAACAACGCGATCCGCAAAGCCACTAAACTTAAAAATAGCTCGGAAAATCCAGGAAATCAGAACTATCAAGGCTTTA
TTGGCAGTAGTCAAACCATGCAGCAGGTTTACCGAACGATCGATTCTGCCGCATCGAGCAAAGCCAGTATTTTCATCACA
GGTGAAAGTGGTACGGGTAAAGAGGTATGTGCTGAAGCCATTCACGCTGCGAGTAAGCGTGGCGATAAGCCATTTATCGC
AATTAACTGTGCAGCGATTCCTAAAGATTTGATTGAGAGTGAATTGTTTGGTCACGTTAAAGGGGCCTTTACTGGAGCAG
CAACGGATCGACAAGGCGCAGCTGAACTTGCAGATGGTGGAACCCTGTTCCTCGATGAATTGTGCGAAATGGACTTAGAG
CTGCAAACTAAGCTACTCCGCTTCATTCAAACTGGTACCTTTCAAAAAGTCGGCTCTTCGAAGATGAAGAGTGTGGATGT
TCGTTTCGTATGTGCAACGAACCGCGACCCTTGGAAGGAAGTTCAAGAAGGTCGCTTTAGAGAAGATTTATACTACCGTT
TATATGTGATCCCACTGCATTTGCCGCCATTGCGTGAGCGTGGTGAAGATGTCATCGAAATTGCATATTCATTGCTGGGT
TATATGTCTGTCGAAGAAGGCAAGGCGTTCGTACGTTTTGCTGAAGAAGTACTCGATCGCTTTAATCAATATGAGTGGCC
GGGGAACGTTCGTCAGTTACAAAATGTGTTGCGAAATGTAGTGGTACTGAATAATGGTAAAGAGATTACTCTCAATATGC
TTCCACCGCCATTGAACCAACCAATTGAAAACAGTCTCCGTTTAAAAGAGAAGCAGAACGAAGACATCACGGTAAAAGAT
ATTTTCCCATTGTGGATCACTGAGAAAACGGCTATCGAACAGGCCATCAAAGCGTGTGACGGTAACGTTCCTCGTGCGGC
AGGTTTCTTGGATGTCAGTCCATCGACGATATACCGAAAATTGCAAACATGGAATGCGAAGCAGTAA

Upstream 100 bases:

>100_bases
CATAATGCAAAGCGGAATGCGATTATAATAAGAAATGCAAATTATAAATGGCTAGGATATGCAATCAAAAACGTTAGATA
ACAAATCGAAGTATTTGTTA

Downstream 100 bases:

>100_bases
TCACCAAATAATAAGAAAAGAGCAATGATGATAATATTAAATCAGCAAAAAGTTGATGAGCTTGCCGGTGAAATAGGGCA
AGAGAATGTCCCAGTGTTAC

Product: LuxO repressor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MVEDTASVAALYRSYLTPLEIDINIVGTGRDAIESLNHRIPDLILLDLRLPDMTGMDVLFAVKQKYPEVPVIFMTAHGSI
DTAVEAMRHGSQDFLIKPCEADRLRITVNNAIRKATKLKNSSENPGNQNYQGFIGSSQTMQQVYRTIDSAASSKASIFIT
GESGTGKEVCAEAIHAASKRGDKPFIAINCAAIPKDLIESELFGHVKGAFTGAATDRQGAAELADGGTLFLDELCEMDLE
LQTKLLRFIQTGTFQKVGSSKMKSVDVRFVCATNRDPWKEVQEGRFREDLYYRLYVIPLHLPPLRERGEDVIEIAYSLLG
YMSVEEGKAFVRFAEEVLDRFNQYEWPGNVRQLQNVLRNVVVLNNGKEITLNMLPPPLNQPIENSLRLKEKQNEDITVKD
IFPLWITEKTAIEQAIKACDGNVPRAAGFLDVSPSTIYRKLQTWNAKQ

Sequences:

>Translated_448_residues
MVEDTASVAALYRSYLTPLEIDINIVGTGRDAIESLNHRIPDLILLDLRLPDMTGMDVLFAVKQKYPEVPVIFMTAHGSI
DTAVEAMRHGSQDFLIKPCEADRLRITVNNAIRKATKLKNSSENPGNQNYQGFIGSSQTMQQVYRTIDSAASSKASIFIT
GESGTGKEVCAEAIHAASKRGDKPFIAINCAAIPKDLIESELFGHVKGAFTGAATDRQGAAELADGGTLFLDELCEMDLE
LQTKLLRFIQTGTFQKVGSSKMKSVDVRFVCATNRDPWKEVQEGRFREDLYYRLYVIPLHLPPLRERGEDVIEIAYSLLG
YMSVEEGKAFVRFAEEVLDRFNQYEWPGNVRQLQNVLRNVVVLNNGKEITLNMLPPPLNQPIENSLRLKEKQNEDITVKD
IFPLWITEKTAIEQAIKACDGNVPRAAGFLDVSPSTIYRKLQTWNAKQ
>Mature_448_residues
MVEDTASVAALYRSYLTPLEIDINIVGTGRDAIESLNHRIPDLILLDLRLPDMTGMDVLFAVKQKYPEVPVIFMTAHGSI
DTAVEAMRHGSQDFLIKPCEADRLRITVNNAIRKATKLKNSSENPGNQNYQGFIGSSQTMQQVYRTIDSAASSKASIFIT
GESGTGKEVCAEAIHAASKRGDKPFIAINCAAIPKDLIESELFGHVKGAFTGAATDRQGAAELADGGTLFLDELCEMDLE
LQTKLLRFIQTGTFQKVGSSKMKSVDVRFVCATNRDPWKEVQEGRFREDLYYRLYVIPLHLPPLRERGEDVIEIAYSLLG
YMSVEEGKAFVRFAEEVLDRFNQYEWPGNVRQLQNVLRNVVVLNNGKEITLNMLPPPLNQPIENSLRLKEKQNEDITVKD
IFPLWITEKTAIEQAIKACDGNVPRAAGFLDVSPSTIYRKLQTWNAKQ

Specific function: Acts negatively to control the expression of luminescence. At low cell density, luxO is phosphorylated, and together with sigma-54, causes repression of the luxCDABEGH operon. This repression could be indirect, luxO could activate a negative regulator of

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790437, Length=443, Percent_Identity=40.1805869074492, Blast_Score=317, Evalue=1e-87,
Organism=Escherichia coli, GI1790299, Length=460, Percent_Identity=39.1304347826087, Blast_Score=315, Evalue=4e-87,
Organism=Escherichia coli, GI1788550, Length=448, Percent_Identity=38.3928571428571, Blast_Score=309, Evalue=3e-85,
Organism=Escherichia coli, GI1788905, Length=344, Percent_Identity=42.7325581395349, Blast_Score=273, Evalue=2e-74,
Organism=Escherichia coli, GI87082117, Length=362, Percent_Identity=40.6077348066298, Blast_Score=249, Evalue=2e-67,
Organism=Escherichia coli, GI1789233, Length=326, Percent_Identity=41.4110429447853, Blast_Score=235, Evalue=4e-63,
Organism=Escherichia coli, GI1789087, Length=315, Percent_Identity=40.3174603174603, Blast_Score=233, Evalue=3e-62,
Organism=Escherichia coli, GI87082152, Length=243, Percent_Identity=48.1481481481481, Blast_Score=231, Evalue=7e-62,
Organism=Escherichia coli, GI1786524, Length=324, Percent_Identity=38.2716049382716, Blast_Score=205, Evalue=5e-54,
Organism=Escherichia coli, GI1787583, Length=261, Percent_Identity=39.0804597701149, Blast_Score=197, Evalue=8e-52,
Organism=Escherichia coli, GI87081872, Length=305, Percent_Identity=37.0491803278689, Blast_Score=187, Evalue=8e-49,
Organism=Escherichia coli, GI1789828, Length=244, Percent_Identity=36.0655737704918, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI87081858, Length=395, Percent_Identity=26.0759493670886, Blast_Score=127, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 50076; Mature: 50076

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEDTASVAALYRSYLTPLEIDINIVGTGRDAIESLNHRIPDLILLDLRLPDMTGMDVLF
CCCCHHHHHHHHHHHCCCEEEEEEEEECCHHHHHHHHCCCCCEEEEEEECCCCCCHHHHH
AVKQKYPEVPVIFMTAHGSIDTAVEAMRHGSQDFLIKPCEADRLRITVNNAIRKATKLKN
HHHHHCCCCCEEEEEECCCHHHHHHHHHCCCCCCEEECCCCCEEEEEHHHHHHHHHHHCC
SSENPGNQNYQGFIGSSQTMQQVYRTIDSAASSKASIFITGESGTGKEVCAEAIHAASKR
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHC
GDKPFIAINCAAIPKDLIESELFGHVKGAFTGAATDRQGAAELADGGTLFLDELCEMDLE
CCCCEEEEEEHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCHHHHHHHHCCCHH
LQTKLLRFIQTGTFQKVGSSKMKSVDVRFVCATNRDPWKEVQEGRFREDLYYRLYVIPLH
HHHHHHHHHHCCCHHHHCCCHHCEEEEEEEEECCCCHHHHHHCCCCHHCEEEEEEEEEEC
LPPLRERGEDVIEIAYSLLGYMSVEEGKAFVRFAEEVLDRFNQYEWPGNVRQLQNVLRNV
CCCHHHHCHHHHHHHHHHHHHHCHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHE
VVLNNGKEITLNMLPPPLNQPIENSLRLKEKQNEDITVKDIFPLWITEKTAIEQAIKACD
EEECCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEEECEEEECCHHHHHHHHHHHC
GNVPRAAGFLDVSPSTIYRKLQTWNAKQ
CCCCCCCCEEECCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MVEDTASVAALYRSYLTPLEIDINIVGTGRDAIESLNHRIPDLILLDLRLPDMTGMDVLF
CCCCHHHHHHHHHHHCCCEEEEEEEEECCHHHHHHHHCCCCCEEEEEEECCCCCCHHHHH
AVKQKYPEVPVIFMTAHGSIDTAVEAMRHGSQDFLIKPCEADRLRITVNNAIRKATKLKN
HHHHHCCCCCEEEEEECCCHHHHHHHHHCCCCCCEEECCCCCEEEEEHHHHHHHHHHHCC
SSENPGNQNYQGFIGSSQTMQQVYRTIDSAASSKASIFITGESGTGKEVCAEAIHAASKR
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHC
GDKPFIAINCAAIPKDLIESELFGHVKGAFTGAATDRQGAAELADGGTLFLDELCEMDLE
CCCCEEEEEEHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCHHHHHHHHCCCHH
LQTKLLRFIQTGTFQKVGSSKMKSVDVRFVCATNRDPWKEVQEGRFREDLYYRLYVIPLH
HHHHHHHHHHCCCHHHHCCCHHCEEEEEEEEECCCCHHHHHHCCCCHHCEEEEEEEEEEC
LPPLRERGEDVIEIAYSLLGYMSVEEGKAFVRFAEEVLDRFNQYEWPGNVRQLQNVLRNV
CCCHHHHCHHHHHHHHHHHHHHCHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHE
VVLNNGKEITLNMLPPPLNQPIENSLRLKEKQNEDITVKDIFPLWITEKTAIEQAIKACD
EEECCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEEECEEEECCHHHHHHHHHHHC
GNVPRAAGFLDVSPSTIYRKLQTWNAKQ
CCCCCCCCEEECCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8065259 [H]