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The map label for this gene is sppA [H]

Identifier: 218708935

GI number: 218708935

Start: 963095

End: 964945

Strand: Reverse

Name: sppA [H]

Synonym: VS_0937

Alternate gene names: 218708935

Gene position: 964945-963095 (Counterclockwise)

Preceding gene: 218708939

Following gene: 218708934

Centisome position: 29.25

GC content: 46.03

Gene sequence:

>1851_bases
ATGAAAAAAATATTCAAATTTATAGGCATGATCTTTAAAGGGATTTGGAAGCTCATCACGTTTGTGCGTCTTGCGCTGGT
TAACCTTTTCTTTTTACTCAGTATCGCCATCATCTACTTTGTATACTTCCACTCAGACACAACTCAACCCACTGTTCCAC
AGGAATCGGCTTTAGTGCTTAACCTGTCTGGTCCTATCGTGGAGCAAAGTCGCTATATCAATCCGATGGATTCAGTCACG
GGTTCACTGCTTGGCAAAGACCTTCCAAAAGAGAACGTCCTGTTTGATATTGTTGAAACGATTCGCTACGCCAAAGACGA
TGACAAGGTAACTGGTATCGTTTTAGCACTCAAAGAACTGCCAGAAACCAATCTGACCAAGCTGCGCTACATAGCTAAAG
CACTTAACGAGTTCAAAGCAACTGGCAAACCAATTTATGCGGTGGGTGACTTTTATAACCAAAGCCAATACTACCTAGCC
AGCTACGCAACTAAAGTCTTCTTATCGCCTGACGGCGGCGTGCTGCTTAAAGGCTACAGTGCTTACTCGCTATTCTACAA
AACGTTATTAGAGAAGCTTGACGTAAATACACATGTATTCCGCGTCGGTACTTATAAGTCTGCAATCGAACCTTTCATTC
GCGACGACATGTCAGATGCAGCGAAAGAATCTGCTTCTCGTTGGTTAGGTCAACTGTGGGGAGCATACGTTGATGACGTA
AGCAACAACCGCCAGATCGATGCTAAAACCTTGAACCCAAGCATGGACTCTTTCTTAAAAGACCTTGAGTCGGTTGATGG
CGATATCGCAAAACTGGCAGAGAAGCTTGGTTTAGTTGACGAACTGGCAACTCGCCAACAAGTTCGTCTAGAGCTCGCAG
ACGTCTTCGGCAGTGACGGTCAAGACAGCTATAACGCGTTGGGGTACTACGAGTACCGTACAACCATGCTTCCTGACCTA
ACAAGTGAATCGCACGATGTCGCTGTAATTGTCGCTAGCGGCGCAATTATGGATGGCAAGCAGCCACGTGGTACTGTGGG
TGGAGATACCACTGCCGCCTTACTTCGCCAAGCGCGCAACGACGACAAAGTAAAAGCGGTTGTACTTCGTGTAGACAGCC
CAGGTGGTAGCGCATTTGCTTCAGAAGTTATCCGCAATGAAATAGAAGCGATTAAGCAAGCGGGCAAGCCGGTTGTTGTC
TCGATGTCTAGCCTTGCCGCTTCGGGTGGTTACTGGATCTCGATGGGTGCGGATAAGATTTTGGCTCAACCAACCACACT
AACGGGTTCAATTGGTATCTTCAGTGTTATCACAACCTTCGAAAAAGGACTGAACGATATTGGTGTTTACACTGATGGCG
TTGGTACGTCGCCTTTCTCTGGCCTTGGTATTACAACAGGGCTTAGCGAAGGCGCGAAAGATGCGTTCCAAATGGGCATT
GAAAACGGTTACCGCCGATTCATCAGCCTAGTTGGAGAAAACCGTGGCATGGAAGTCGATGCTGTCGACAAGATTGCTCA
AGGCCGAGTATGGACAGGCCAAGATGCAATCGAGAAAGGTCTAGTCGATGAAATTGGTGATTTTGATGATGCGATTACAG
CTGCGGCTTCACTTGCAGAGCTTGAGACCTACAACATTTACTGGGTAGAAGAGCCTCTTTCAGCCACTGAACTATTTATT
CAAGAATTTATGAACCAAGTTCAGATGTCGATAGGCCTTGATATTCAGTCAATGATTCCAAGCAGTTTACAGCCTGTTAC
TCAACAGTTAGCTCAAGATAGCCAACTGTTAGGTAACTTTAACGACCCACAAGGTCGATATGCTTTTTGCTTAAACTGCC
AAGTTCAATAA

Upstream 100 bases:

>100_bases
TTGAGCTAGGGAATTATCGTAGGATATCAAGCAGTTCATATTATTGAGATTAATGGTACTACAGCTTGTTAAAGCGACGT
GATCTCACTGCGGAGACAGA

Downstream 100 bases:

>100_bases
GATAAGCTCAACGCTTTGTTATCAAGAGGCGCCTCTGTGTGAGGCGCCTCTTTTTATTGCATGATAATTCGCTATAATCG
CCCCCCTGTTCCCTATATCA

Product: protease IV

Products: NA

Alternate protein names: Endopeptidase IV; Protease IV; Signal peptide peptidase [H]

Number of amino acids: Translated: 616; Mature: 616

Protein sequence:

>616_residues
MKKIFKFIGMIFKGIWKLITFVRLALVNLFFLLSIAIIYFVYFHSDTTQPTVPQESALVLNLSGPIVEQSRYINPMDSVT
GSLLGKDLPKENVLFDIVETIRYAKDDDKVTGIVLALKELPETNLTKLRYIAKALNEFKATGKPIYAVGDFYNQSQYYLA
SYATKVFLSPDGGVLLKGYSAYSLFYKTLLEKLDVNTHVFRVGTYKSAIEPFIRDDMSDAAKESASRWLGQLWGAYVDDV
SNNRQIDAKTLNPSMDSFLKDLESVDGDIAKLAEKLGLVDELATRQQVRLELADVFGSDGQDSYNALGYYEYRTTMLPDL
TSESHDVAVIVASGAIMDGKQPRGTVGGDTTAALLRQARNDDKVKAVVLRVDSPGGSAFASEVIRNEIEAIKQAGKPVVV
SMSSLAASGGYWISMGADKILAQPTTLTGSIGIFSVITTFEKGLNDIGVYTDGVGTSPFSGLGITTGLSEGAKDAFQMGI
ENGYRRFISLVGENRGMEVDAVDKIAQGRVWTGQDAIEKGLVDEIGDFDDAITAAASLAELETYNIYWVEEPLSATELFI
QEFMNQVQMSIGLDIQSMIPSSLQPVTQQLAQDSQLLGNFNDPQGRYAFCLNCQVQ

Sequences:

>Translated_616_residues
MKKIFKFIGMIFKGIWKLITFVRLALVNLFFLLSIAIIYFVYFHSDTTQPTVPQESALVLNLSGPIVEQSRYINPMDSVT
GSLLGKDLPKENVLFDIVETIRYAKDDDKVTGIVLALKELPETNLTKLRYIAKALNEFKATGKPIYAVGDFYNQSQYYLA
SYATKVFLSPDGGVLLKGYSAYSLFYKTLLEKLDVNTHVFRVGTYKSAIEPFIRDDMSDAAKESASRWLGQLWGAYVDDV
SNNRQIDAKTLNPSMDSFLKDLESVDGDIAKLAEKLGLVDELATRQQVRLELADVFGSDGQDSYNALGYYEYRTTMLPDL
TSESHDVAVIVASGAIMDGKQPRGTVGGDTTAALLRQARNDDKVKAVVLRVDSPGGSAFASEVIRNEIEAIKQAGKPVVV
SMSSLAASGGYWISMGADKILAQPTTLTGSIGIFSVITTFEKGLNDIGVYTDGVGTSPFSGLGITTGLSEGAKDAFQMGI
ENGYRRFISLVGENRGMEVDAVDKIAQGRVWTGQDAIEKGLVDEIGDFDDAITAAASLAELETYNIYWVEEPLSATELFI
QEFMNQVQMSIGLDIQSMIPSSLQPVTQQLAQDSQLLGNFNDPQGRYAFCLNCQVQ
>Mature_616_residues
MKKIFKFIGMIFKGIWKLITFVRLALVNLFFLLSIAIIYFVYFHSDTTQPTVPQESALVLNLSGPIVEQSRYINPMDSVT
GSLLGKDLPKENVLFDIVETIRYAKDDDKVTGIVLALKELPETNLTKLRYIAKALNEFKATGKPIYAVGDFYNQSQYYLA
SYATKVFLSPDGGVLLKGYSAYSLFYKTLLEKLDVNTHVFRVGTYKSAIEPFIRDDMSDAAKESASRWLGQLWGAYVDDV
SNNRQIDAKTLNPSMDSFLKDLESVDGDIAKLAEKLGLVDELATRQQVRLELADVFGSDGQDSYNALGYYEYRTTMLPDL
TSESHDVAVIVASGAIMDGKQPRGTVGGDTTAALLRQARNDDKVKAVVLRVDSPGGSAFASEVIRNEIEAIKQAGKPVVV
SMSSLAASGGYWISMGADKILAQPTTLTGSIGIFSVITTFEKGLNDIGVYTDGVGTSPFSGLGITTGLSEGAKDAFQMGI
ENGYRRFISLVGENRGMEVDAVDKIAQGRVWTGQDAIEKGLVDEIGDFDDAITAAASLAELETYNIYWVEEPLSATELFI
QEFMNQVQMSIGLDIQSMIPSSLQPVTQQLAQDSQLLGNFNDPQGRYAFCLNCQVQ

Specific function: Digestion of the cleaved signal peptides. This activity is necessary to maintain proper secretion of mature proteins across the membrane [H]

COG id: COG0616

COG function: function code OU; Periplasmic serine proteases (ClpP class)

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S49 family [H]

Homologues:

Organism=Escherichia coli, GI1788064, Length=622, Percent_Identity=49.0353697749196, Blast_Score=560, Evalue=1e-161,
Organism=Escherichia coli, GI1787527, Length=170, Percent_Identity=32.9411764705882, Blast_Score=92, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004634
- InterPro:   IPR004635
- InterPro:   IPR002142 [H]

Pfam domain/function: PF01343 Peptidase_S49 [H]

EC number: 3.4.21.-

Molecular weight: Translated: 67516; Mature: 67516

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIFKFIGMIFKGIWKLITFVRLALVNLFFLLSIAIIYFVYFHSDTTQPTVPQESALVL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEE
NLSGPIVEQSRYINPMDSVTGSLLGKDLPKENVLFDIVETIRYAKDDDKVTGIVLALKEL
ECCCCCHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHC
PETNLTKLRYIAKALNEFKATGKPIYAVGDFYNQSQYYLASYATKVFLSPDGGVLLKGYS
CCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHEECCEEEEECCCCCEEEECHH
AYSLFYKTLLEKLDVNTHVFRVGTYKSAIEPFIRDDMSDAAKESASRWLGQLWGAYVDDV
HHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SNNRQIDAKTLNPSMDSFLKDLESVDGDIAKLAEKLGLVDELATRQQVRLELADVFGSDG
CCCCEEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCC
QDSYNALGYYEYRTTMLPDLTSESHDVAVIVASGAIMDGKQPRGTVGGDTTAALLRQARN
CCCCCCCEEEEEHHHHCCCCCCCCCCEEEEEECCCEECCCCCCCCCCCHHHHHHHHHHCC
DDKVKAVVLRVDSPGGSAFASEVIRNEIEAIKQAGKPVVVSMSSLAASGGYWISMGADKI
CCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHCCCCEEEEECCCHH
LAQPTTLTGSIGIFSVITTFEKGLNDIGVYTDGVGTSPFSGLGITTGLSEGAKDAFQMGI
HCCCCCCCCCHHHHHHHHHHHHCCHHCCCEECCCCCCCCCCCCCCCCCCHHHHHHHHHHH
ENGYRRFISLVGENRGMEVDAVDKIAQGRVWTGQDAIEKGLVDEIGDFDDAITAAASLAE
HHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCHHHHHHCHHHHHCCHHHHHHHHHHHHH
LETYNIYWVEEPLSATELFIQEFMNQVQMSIGLDIQSMIPSSLQPVTQQLAQDSQLLGNF
HEEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHCCC
NDPQGRYAFCLNCQVQ
CCCCCCEEEEEEEEEC
>Mature Secondary Structure
MKKIFKFIGMIFKGIWKLITFVRLALVNLFFLLSIAIIYFVYFHSDTTQPTVPQESALVL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEE
NLSGPIVEQSRYINPMDSVTGSLLGKDLPKENVLFDIVETIRYAKDDDKVTGIVLALKEL
ECCCCCHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHC
PETNLTKLRYIAKALNEFKATGKPIYAVGDFYNQSQYYLASYATKVFLSPDGGVLLKGYS
CCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHEECCEEEEECCCCCEEEECHH
AYSLFYKTLLEKLDVNTHVFRVGTYKSAIEPFIRDDMSDAAKESASRWLGQLWGAYVDDV
HHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SNNRQIDAKTLNPSMDSFLKDLESVDGDIAKLAEKLGLVDELATRQQVRLELADVFGSDG
CCCCEEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCC
QDSYNALGYYEYRTTMLPDLTSESHDVAVIVASGAIMDGKQPRGTVGGDTTAALLRQARN
CCCCCCCEEEEEHHHHCCCCCCCCCCEEEEEECCCEECCCCCCCCCCCHHHHHHHHHHCC
DDKVKAVVLRVDSPGGSAFASEVIRNEIEAIKQAGKPVVVSMSSLAASGGYWISMGADKI
CCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHCCCCEEEEECCCHH
LAQPTTLTGSIGIFSVITTFEKGLNDIGVYTDGVGTSPFSGLGITTGLSEGAKDAFQMGI
HCCCCCCCCCHHHHHHHHHHHHCCHHCCCEECCCCCCCCCCCCCCCCCCHHHHHHHHHHH
ENGYRRFISLVGENRGMEVDAVDKIAQGRVWTGQDAIEKGLVDEIGDFDDAITAAASLAE
HHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCHHHHHHCHHHHHCCHHHHHHHHHHHHH
LETYNIYWVEEPLSATELFIQEFMNQVQMSIGLDIQSMIPSSLQPVTQQLAQDSQLLGNF
HEEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHCCC
NDPQGRYAFCLNCQVQ
CCCCCCEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3522590; 9097039; 9278503; 7973728 [H]