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The map label for this gene is ansA [H]

Identifier: 218708934

GI number: 218708934

Start: 961960

End: 962976

Strand: Reverse

Name: ansA [H]

Synonym: VS_0936

Alternate gene names: 218708934

Gene position: 962976-961960 (Counterclockwise)

Preceding gene: 218708935

Following gene: 218708932

Centisome position: 29.19

GC content: 45.53

Gene sequence:

>1017_bases
ATGGAAAGAAAACACATCTATATCGCGTATACCGGCGGCACAATTGGCATGCAGAAGTCTTTGGACCACGGCTATGTCCC
AGTCGCAGGTTTTATGGATAAGCAGCTAGCTGGCATGCCTGAATTCCATCGCCCAGAAATGCCTGAGTACACCATTCATG
AATACTCTCCATTAATGGACTCTTCAGATATGACGCCACTTGATTGGCAGACTATCGCTGATGATATTCGCGCGAACTAC
GATAAGTACGATGGTTTCGTTATCCTGCACGGTACAGACACTATGGCTTACACCGCCTCAGCTCTGTCATTCATGTTAGA
AAACCTCGGCAAACCTGTGATTGTTACGGGCTCTCAGATCCCATTAGCAGAATTACGTTCAGACGGACAAGCAAACCTGC
TTAATGCTCTGCACATTGCAGCGAACTATCCGATCAACGAAGTGACACTGTTCTTCAACAACAAGTTGATGCGCGGTAAC
CGCAGTACAAAATCACACGCTGATGGCTTCAATGCGTTTACCTCTCCAAACCTAACTCCATTGCTAGAAGCGGGTATCAA
TATCCAGATCAGCAATAACGTTAAGGTAAACGAACAACCTGAAGGTGCTTTCAAGGTTCATAACATTACTCCGCAACCCA
TCGGTGTAATTACAATGTACCCAGGAATCTCGCATGAGGTGATTCGCAACACGCTACTACAGCCTGTAAATGCAATGATT
CTGCTTACTTTTGGTGTTGGTAACGCACCGCAAAATCAAGAACTACTTCAACACTTAAAAGATGCCTCAGAGCGAGGTGT
GATTGTGGTGAACCTAACTCAGTGTTTGGCAGGTAAAGTCAACATGGGTGGTTACGCGACGGGTTGTGCACTAGCAGAAT
CGGGCGTTATCAGTGGTTACGATATGACACCAGAAGCAGCACTGGCGAAATTACATTATCTATTGAGCCAAGATTTAAGC
TACGAAGAAGTGAAGGCTCAAATGCAACAAGTGTTACGCGGTGAGATGAGCTTATAA

Upstream 100 bases:

>100_bases
TTATCAAGAGGCGCCTCTGTGTGAGGCGCCTCTTTTTATTGCATGATAATTCGCTATAATCGCCCCCCTGTTCCCTATAT
CACACTAAGTATTAATCGCC

Downstream 100 bases:

>100_bases
GCCTATCCTGCGATTCGTTTAGCCGAGGGCTTACCTTTAATCAAGCCCCTCACGTAAACCTGAACTAAATCGTATTATTG
TTTATAAATAAACAGCTTAA

Product: cytoplasmic asparaginase I

Products: NA

Alternate protein names: L-asparaginase I; L-ASNase I; L-asparagine amidohydrolase I [H]

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MERKHIYIAYTGGTIGMQKSLDHGYVPVAGFMDKQLAGMPEFHRPEMPEYTIHEYSPLMDSSDMTPLDWQTIADDIRANY
DKYDGFVILHGTDTMAYTASALSFMLENLGKPVIVTGSQIPLAELRSDGQANLLNALHIAANYPINEVTLFFNNKLMRGN
RSTKSHADGFNAFTSPNLTPLLEAGINIQISNNVKVNEQPEGAFKVHNITPQPIGVITMYPGISHEVIRNTLLQPVNAMI
LLTFGVGNAPQNQELLQHLKDASERGVIVVNLTQCLAGKVNMGGYATGCALAESGVISGYDMTPEAALAKLHYLLSQDLS
YEEVKAQMQQVLRGEMSL

Sequences:

>Translated_338_residues
MERKHIYIAYTGGTIGMQKSLDHGYVPVAGFMDKQLAGMPEFHRPEMPEYTIHEYSPLMDSSDMTPLDWQTIADDIRANY
DKYDGFVILHGTDTMAYTASALSFMLENLGKPVIVTGSQIPLAELRSDGQANLLNALHIAANYPINEVTLFFNNKLMRGN
RSTKSHADGFNAFTSPNLTPLLEAGINIQISNNVKVNEQPEGAFKVHNITPQPIGVITMYPGISHEVIRNTLLQPVNAMI
LLTFGVGNAPQNQELLQHLKDASERGVIVVNLTQCLAGKVNMGGYATGCALAESGVISGYDMTPEAALAKLHYLLSQDLS
YEEVKAQMQQVLRGEMSL
>Mature_338_residues
MERKHIYIAYTGGTIGMQKSLDHGYVPVAGFMDKQLAGMPEFHRPEMPEYTIHEYSPLMDSSDMTPLDWQTIADDIRANY
DKYDGFVILHGTDTMAYTASALSFMLENLGKPVIVTGSQIPLAELRSDGQANLLNALHIAANYPINEVTLFFNNKLMRGN
RSTKSHADGFNAFTSPNLTPLLEAGINIQISNNVKVNEQPEGAFKVHNITPQPIGVITMYPGISHEVIRNTLLQPVNAMI
LLTFGVGNAPQNQELLQHLKDASERGVIVVNLTQCLAGKVNMGGYATGCALAESGVISGYDMTPEAALAKLHYLLSQDLS
YEEVKAQMQQVLRGEMSL

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=358, Percent_Identity=45.5307262569832, Blast_Score=277, Evalue=1e-74,
Organism=Escherichia coli, GI1788065, Length=341, Percent_Identity=71.8475073313783, Blast_Score=510, Evalue=1e-146,
Organism=Escherichia coli, GI1789327, Length=280, Percent_Identity=27.5, Blast_Score=83, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17558280, Length=360, Percent_Identity=46.9444444444444, Blast_Score=306, Evalue=1e-83,
Organism=Caenorhabditis elegans, GI17558278, Length=360, Percent_Identity=46.9444444444444, Blast_Score=306, Evalue=1e-83,
Organism=Caenorhabditis elegans, GI193207372, Length=360, Percent_Identity=46.9444444444444, Blast_Score=306, Evalue=1e-83,
Organism=Caenorhabditis elegans, GI193207374, Length=360, Percent_Identity=47.5, Blast_Score=306, Evalue=1e-83,
Organism=Drosophila melanogaster, GI24639609, Length=364, Percent_Identity=43.1318681318681, Blast_Score=291, Evalue=6e-79,
Organism=Drosophila melanogaster, GI24645530, Length=357, Percent_Identity=44.2577030812325, Blast_Score=280, Evalue=1e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006033
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 37103; Mature: 37103

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERKHIYIAYTGGTIGMQKSLDHGYVPVAGFMDKQLAGMPEFHRPEMPEYTIHEYSPLMD
CCCCEEEEEECCCCCCCHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCC
SSDMTPLDWQTIADDIRANYDKYDGFVILHGTDTMAYTASALSFMLENLGKPVIVTGSQI
CCCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEECCCC
PLAELRSDGQANLLNALHIAANYPINEVTLFFNNKLMRGNRSTKSHADGFNAFTSPNLTP
CHHHHHCCCCHHHHHHHHHHCCCCCCEEEEEECCEEECCCCCCCCCCCCCCCCCCCCCCH
LLEAGINIQISNNVKVNEQPEGAFKVHNITPQPIGVITMYPGISHEVIRNTLLQPVNAMI
HHHCCEEEEECCCEEECCCCCCCEEEECCCCCCEEEEEECCCCCHHHHHHHHHHCCCEEE
LLTFGVGNAPQNQELLQHLKDASERGVIVVNLTQCLAGKVNMGGYATGCALAESGVISGY
EEEECCCCCCCHHHHHHHHHCHHHCCEEEEEEHHHHCCCCCCCCEEHHHHHHCCCCCCCC
DMTPEAALAKLHYLLSQDLSYEEVKAQMQQVLRGEMSL
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MERKHIYIAYTGGTIGMQKSLDHGYVPVAGFMDKQLAGMPEFHRPEMPEYTIHEYSPLMD
CCCCEEEEEECCCCCCCHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCC
SSDMTPLDWQTIADDIRANYDKYDGFVILHGTDTMAYTASALSFMLENLGKPVIVTGSQI
CCCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEECCCC
PLAELRSDGQANLLNALHIAANYPINEVTLFFNNKLMRGNRSTKSHADGFNAFTSPNLTP
CHHHHHCCCCHHHHHHHHHHCCCCCCEEEEEECCEEECCCCCCCCCCCCCCCCCCCCCCH
LLEAGINIQISNNVKVNEQPEGAFKVHNITPQPIGVITMYPGISHEVIRNTLLQPVNAMI
HHHCCEEEEECCCEEECCCCCCCEEEECCCCCCEEEEEECCCCCHHHHHHHHHHCCCEEE
LLTFGVGNAPQNQELLQHLKDASERGVIVVNLTQCLAGKVNMGGYATGCALAESGVISGY
EEEECCCCCCCHHHHHHHHHCHHHCCEEEEEEHHHHCCCCCCCCEEHHHHHHCCCCCCCC
DMTPEAALAKLHYLLSQDLSYEEVKAQMQQVLRGEMSL
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]