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The map label for this gene is rrmA [H]

Identifier: 218708926

GI number: 218708926

Start: 954189

End: 955004

Strand: Reverse

Name: rrmA [H]

Synonym: VS_0928

Alternate gene names: 218708926

Gene position: 955004-954189 (Counterclockwise)

Preceding gene: 218708927

Following gene: 218708925

Centisome position: 28.95

GC content: 45.83

Gene sequence:

>816_bases
ATGACTTACCAATGCCCTTTGTGTCACCAACCTTTATCTCAAAACGATCGTACGTTTAAGTGTGAAAAGAACCATCAGTT
CGACCTAGCGAAAGAAGGCTATGTTAATTTGATGCCAGCACACCACAAACGCTCAAAAGATCCAGGTGACAACAAAGAGA
TGATGCAGGCTCGTCGCCGCTTCCTTGAAGGCAACCACTACGATCCAATGCGCCAAGCCGTTGTTGGTTTATGCGCTAGC
TATCTGCCAGAAACAACCCCTAGCCTATTAGATATCGGTTGTGGTGAAGGTTATTACACCAATGAAATTGCGGTAAACCT
TCACGAAAAGAATGGCGCTACTTTTGGTCTCGATATCTCTAAAATTGCTATCAAATATGCCGCTAAACGCTATCCAGCGG
TCGACTTCTCAGTCGCTTCGAGCCATCGCCTACCCTTTTCTGAAAACAGCTTAGACGGCATTCTACGCATTTACGCGCCT
TGTAAGGCAGAAGAGCTGCAACGCACGATCAAAGACAATGGTGTGGTGATCACCGTGACCCCTGCTAGCCGACACCTTTA
TCAGCTGCGTGATGCGATTTATGATGGCGTTCGTTTGCACGATGAAGATCCAGAGATGATCGAAGGTTTTACTCTTGAGC
ACCAAGAGCAACTAAACTATATGATGGAACTATCGGGGTCAGACGCATTCGACCTGCTACAGATGACGCCGTTTGCTTGG
AAAGCGAGTGAAGAATTTAAACAACAACTCACCGATTCAAAGCAATTCAACTGTGAAGCTGACTTTATGCTACGAGTGTA
CCGCAAACAACTTTAA

Upstream 100 bases:

>100_bases
TTCACTAATTGCCGTAAACCAAGACAGCTGCGTTTATTCTAGCGCCCCACAATGCTTCCTGTTAAAATTTTGGACTTGAA
ATTTCTACCTCGAGTTCGCA

Downstream 100 bases:

>100_bases
TTGATATTGATTATCGTTTGCATTGAAATCTCATCTTGCCTCCTTTAGTATGCGTGTTCTTCAAGGAGGCATTTCATGCA
ACGTATTATTCTTATCGGAT

Product: 23S rRNA methyltransferase A

Products: NA

Alternate protein names: 23S rRNA m1G745 methyltransferase; rRNA (guanine-N(1)-)-methyltransferase [H]

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MTYQCPLCHQPLSQNDRTFKCEKNHQFDLAKEGYVNLMPAHHKRSKDPGDNKEMMQARRRFLEGNHYDPMRQAVVGLCAS
YLPETTPSLLDIGCGEGYYTNEIAVNLHEKNGATFGLDISKIAIKYAAKRYPAVDFSVASSHRLPFSENSLDGILRIYAP
CKAEELQRTIKDNGVVITVTPASRHLYQLRDAIYDGVRLHDEDPEMIEGFTLEHQEQLNYMMELSGSDAFDLLQMTPFAW
KASEEFKQQLTDSKQFNCEADFMLRVYRKQL

Sequences:

>Translated_271_residues
MTYQCPLCHQPLSQNDRTFKCEKNHQFDLAKEGYVNLMPAHHKRSKDPGDNKEMMQARRRFLEGNHYDPMRQAVVGLCAS
YLPETTPSLLDIGCGEGYYTNEIAVNLHEKNGATFGLDISKIAIKYAAKRYPAVDFSVASSHRLPFSENSLDGILRIYAP
CKAEELQRTIKDNGVVITVTPASRHLYQLRDAIYDGVRLHDEDPEMIEGFTLEHQEQLNYMMELSGSDAFDLLQMTPFAW
KASEEFKQQLTDSKQFNCEADFMLRVYRKQL
>Mature_270_residues
TYQCPLCHQPLSQNDRTFKCEKNHQFDLAKEGYVNLMPAHHKRSKDPGDNKEMMQARRRFLEGNHYDPMRQAVVGLCASY
LPETTPSLLDIGCGEGYYTNEIAVNLHEKNGATFGLDISKIAIKYAAKRYPAVDFSVASSHRLPFSENSLDGILRIYAPC
KAEELQRTIKDNGVVITVTPASRHLYQLRDAIYDGVRLHDEDPEMIEGFTLEHQEQLNYMMELSGSDAFDLLQMTPFAWK
ASEEFKQQLTDSKQFNCEADFMLRVYRKQL

Specific function: Specifically methylates the guanosine in position 745 of 23S rRNA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmA family [H]

Homologues:

Organism=Escherichia coli, GI1788125, Length=269, Percent_Identity=55.7620817843866, Blast_Score=322, Evalue=2e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013216
- InterPro:   IPR016718 [H]

Pfam domain/function: PF08241 Methyltransf_11 [H]

EC number: =2.1.1.187 [H]

Molecular weight: Translated: 31116; Mature: 30984

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYQCPLCHQPLSQNDRTFKCEKNHQFDLAKEGYVNLMPAHHKRSKDPGDNKEMMQARRR
CCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCEECCCCHHCCCCCCCCCHHHHHHHHH
FLEGNHYDPMRQAVVGLCASYLPETTPSLLDIGCGEGYYTNEIAVNLHEKNGATFGLDIS
HHCCCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCCCCEEECEEEEEEECCCCCEECCHHH
KIAIKYAAKRYPAVDFSVASSHRLPFSENSLDGILRIYAPCKAEELQRTIKDNGVVITVT
HHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCEEEEEC
PASRHLYQLRDAIYDGVRLHDEDPEMIEGFTLEHQEQLNYMMELSGSDAFDLLQMTPFAW
CCHHHHHHHHHHHHCCCEECCCCHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHCCCCC
KASEEFKQQLTDSKQFNCEADFMLRVYRKQL
CCHHHHHHHHCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
TYQCPLCHQPLSQNDRTFKCEKNHQFDLAKEGYVNLMPAHHKRSKDPGDNKEMMQARRR
CCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCEECCCCHHCCCCCCCCCHHHHHHHHH
FLEGNHYDPMRQAVVGLCASYLPETTPSLLDIGCGEGYYTNEIAVNLHEKNGATFGLDIS
HHCCCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCCCCEEECEEEEEEECCCCCEECCHHH
KIAIKYAAKRYPAVDFSVASSHRLPFSENSLDGILRIYAPCKAEELQRTIKDNGVVITVT
HHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCEEEEEC
PASRHLYQLRDAIYDGVRLHDEDPEMIEGFTLEHQEQLNYMMELSGSDAFDLLQMTPFAW
CCHHHHHHHHHHHHCCCEECCCCHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHCCCCC
KASEEFKQQLTDSKQFNCEADFMLRVYRKQL
CCHHHHHHHHCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7984109; 9097040; 9278503; 9440525 [H]