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The map label for this gene is folC [H]

Identifier: 218708904

GI number: 218708904

Start: 933851

End: 935113

Strand: Direct

Name: folC [H]

Synonym: VS_0905

Alternate gene names: 218708904

Gene position: 933851-935113 (Clockwise)

Preceding gene: 218708903

Following gene: 218708905

Centisome position: 28.3

GC content: 45.92

Gene sequence:

>1263_bases
ATGAGTCAACAACCTATTCCTCAAGCCACATCCTCTTTGGAGATGTGGCTTGATTATTTATCAAACATCCACACAAGCGC
TATTGATCTTGGTTTAGACCGAGTTCAAGCTGTCGCCTCTAAGGCAAACCTCACCAAACCTGCTCAACATGTTATTACTG
TTGCTGGAACCAACGGCAAGGGCTCAACATGTGCACTCATGGAAGCTATTCTATTGGATGCTGGTTACTCTGTTGGTGTC
TACAGCTCCCCTCACTTAATTCGCTATAACGAACGTGTTCGTATCAATGGCAAAGATCTGTCTGATCAAAAGATGGTTCA
GTCTTTCGATTTCATTGAGAAAGAACGTGGTGAAATCAGCCTTAGCTTTTTTGAATATGGCACTTTAGCAGCGTTACGTG
CTTTTCAAACCGAAGCGGTTGACGTTGTATTGTTAGAAGTGGGTCTAGGCGGACGTTTAGACGCGACCAATATCGTTGAG
CATGACGTTTCTGTGATTACTAGCTTGGCTGTCGACCATGTTGACTGGCTAGGTGATGACATTAATGTGATCGGTTTTGA
GAAAGCGGGCATTTATCGTAGCGGTAAACCAGCTATCTGTGGTCAACCCAAGCCACCAGCTACGGTTGCGGCACACGCTG
ATGATATTAACGCTGAGTTCTACCAAGTAGGGATTCAATACACCTATGATGTAGATGGTGATACTTGGAACTGGCGCAGT
GGCGCATTCCAGTTAGAATCGCTACCTATCCCAAGCTTACCGCTGCCGAACGCAGCCACCGCACTGATGGCATTAGGTAC
TTCAGAATTAAGCATTAGTGATGTGAACGTTGTTAACGGTATGAAGAATGCGCAGCTTCCTGGGCGTATGCAGCAAATTA
GCGAACAACCGGTGATTGTGCTTGATGTAGCACACAACCCGCATTCTGCTGAGTACTTTGCGCAGCAAGTAGCGAAGAAG
TATGCAGGCAAAAATTTACACGTTGTAGTCGCCATGCTTCATGACAAAGATATCCCCGCGACATTGGAAGTATTAGCTCC
AATAACAACACATTGGTACCCAGCTTCGCTGAAAGGCCCACGAGCCGCAACAGCCGCTGAATTGTGTCAAAGCTTACCTG
AAGGTGTAGAGCAGTATTCAAACCCTGTTGTAGCGTTTGAAGCGGCTTTAGCTTCTGTTATAGATGACGATGTTGTGTTG
GTTGTCGGTTCTTTTCATACCGTGGGTGAAGTGTTGGAGCATTGGCAGAAAAAAGGAAACTAA

Upstream 100 bases:

>100_bases
GAACGATTCACCGAATGAAGCTGCTTATGAAGTACCAGAAGCGCCAGAAAAAGGGTAAAGTACCTTCTAACAAACCTACT
TAATTATGGTTATGAGTTAG

Downstream 100 bases:

>100_bases
ATGGCAAGTAAATTCCAAAGCCGATTAGTCGGCACCATCATTTTAGTGGCCATTGGCGTGATTGTATTGCCCGATGTGCT
GGATGGTAAGAAGCTCCACT

Product: FolC bifunctional protein

Products: NA

Alternate protein names: Folylpolyglutamate synthase; Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolylpolyglutamate synthase; Tetrahydrofolate synthase; Dihydrofolate synthase [H]

Number of amino acids: Translated: 420; Mature: 419

Protein sequence:

>420_residues
MSQQPIPQATSSLEMWLDYLSNIHTSAIDLGLDRVQAVASKANLTKPAQHVITVAGTNGKGSTCALMEAILLDAGYSVGV
YSSPHLIRYNERVRINGKDLSDQKMVQSFDFIEKERGEISLSFFEYGTLAALRAFQTEAVDVVLLEVGLGGRLDATNIVE
HDVSVITSLAVDHVDWLGDDINVIGFEKAGIYRSGKPAICGQPKPPATVAAHADDINAEFYQVGIQYTYDVDGDTWNWRS
GAFQLESLPIPSLPLPNAATALMALGTSELSISDVNVVNGMKNAQLPGRMQQISEQPVIVLDVAHNPHSAEYFAQQVAKK
YAGKNLHVVVAMLHDKDIPATLEVLAPITTHWYPASLKGPRAATAAELCQSLPEGVEQYSNPVVAFEAALASVIDDDVVL
VVGSFHTVGEVLEHWQKKGN

Sequences:

>Translated_420_residues
MSQQPIPQATSSLEMWLDYLSNIHTSAIDLGLDRVQAVASKANLTKPAQHVITVAGTNGKGSTCALMEAILLDAGYSVGV
YSSPHLIRYNERVRINGKDLSDQKMVQSFDFIEKERGEISLSFFEYGTLAALRAFQTEAVDVVLLEVGLGGRLDATNIVE
HDVSVITSLAVDHVDWLGDDINVIGFEKAGIYRSGKPAICGQPKPPATVAAHADDINAEFYQVGIQYTYDVDGDTWNWRS
GAFQLESLPIPSLPLPNAATALMALGTSELSISDVNVVNGMKNAQLPGRMQQISEQPVIVLDVAHNPHSAEYFAQQVAKK
YAGKNLHVVVAMLHDKDIPATLEVLAPITTHWYPASLKGPRAATAAELCQSLPEGVEQYSNPVVAFEAALASVIDDDVVL
VVGSFHTVGEVLEHWQKKGN
>Mature_419_residues
SQQPIPQATSSLEMWLDYLSNIHTSAIDLGLDRVQAVASKANLTKPAQHVITVAGTNGKGSTCALMEAILLDAGYSVGVY
SSPHLIRYNERVRINGKDLSDQKMVQSFDFIEKERGEISLSFFEYGTLAALRAFQTEAVDVVLLEVGLGGRLDATNIVEH
DVSVITSLAVDHVDWLGDDINVIGFEKAGIYRSGKPAICGQPKPPATVAAHADDINAEFYQVGIQYTYDVDGDTWNWRSG
AFQLESLPIPSLPLPNAATALMALGTSELSISDVNVVNGMKNAQLPGRMQQISEQPVIVLDVAHNPHSAEYFAQQVAKKY
AGKNLHVVVAMLHDKDIPATLEVLAPITTHWYPASLKGPRAATAAELCQSLPEGVEQYSNPVVAFEAALASVIDDDVVLV
VGSFHTVGEVLEHWQKKGN

Specific function: Conversion of folates to polyglutamate derivatives [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=337, Percent_Identity=30.5637982195846, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI66932990, Length=337, Percent_Identity=30.5637982195846, Blast_Score=134, Evalue=2e-31,
Organism=Escherichia coli, GI1788654, Length=407, Percent_Identity=51.3513513513513, Blast_Score=403, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17553148, Length=455, Percent_Identity=28.3516483516484, Blast_Score=141, Evalue=8e-34,
Organism=Caenorhabditis elegans, GI17553150, Length=455, Percent_Identity=28.3516483516484, Blast_Score=140, Evalue=9e-34,
Organism=Caenorhabditis elegans, GI71984923, Length=455, Percent_Identity=28.3516483516484, Blast_Score=140, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6323760, Length=347, Percent_Identity=28.5302593659942, Blast_Score=123, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6324815, Length=178, Percent_Identity=35.3932584269663, Blast_Score=107, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24641571, Length=277, Percent_Identity=33.2129963898917, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24581568, Length=159, Percent_Identity=40.251572327044, Blast_Score=102, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17; =6.3.2.12 [H]

Molecular weight: Translated: 45398; Mature: 45267

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQQPIPQATSSLEMWLDYLSNIHTSAIDLGLDRVQAVASKANLTKPAQHVITVAGTNGK
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCC
GSTCALMEAILLDAGYSVGVYSSPHLIRYNERVRINGKDLSDQKMVQSFDFIEKERGEIS
CCHHHHHHHHHHHCCCCCCCCCCCCEEEECCEEEECCCCCCHHHHHHHHHHHHHCCCCEE
LSFFEYGTLAALRAFQTEAVDVVLLEVGLGGRLDATNIVEHDVSVITSLAVDHVDWLGDD
EEEEHHHHHHHHHHHHHHCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
INVIGFEKAGIYRSGKPAICGQPKPPATVAAHADDINAEFYQVGIQYTYDVDGDTWNWRS
CEEEEEECCCCEECCCCCCCCCCCCCCEEEECCCCCCCEEEEEEEEEEEECCCCCCCCCC
GAFQLESLPIPSLPLPNAATALMALGTSELSISDVNVVNGMKNAQLPGRMQQISEQPVIV
CCEEEECCCCCCCCCCCHHHHHHHCCCCCCEECCHHHHCCCCCCCCCCHHHHHCCCCEEE
LDVAHNPHSAEYFAQQVAKKYAGKNLHVVVAMLHDKDIPATLEVLAPITTHWYPASLKGP
EEECCCCCHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHCEECCCCCCC
RAATAAELCQSLPEGVEQYSNPVVAFEAALASVIDDDVVLVVGSFHTVGEVLEHWQKKGN
CHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SQQPIPQATSSLEMWLDYLSNIHTSAIDLGLDRVQAVASKANLTKPAQHVITVAGTNGK
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCC
GSTCALMEAILLDAGYSVGVYSSPHLIRYNERVRINGKDLSDQKMVQSFDFIEKERGEIS
CCHHHHHHHHHHHCCCCCCCCCCCCEEEECCEEEECCCCCCHHHHHHHHHHHHHCCCCEE
LSFFEYGTLAALRAFQTEAVDVVLLEVGLGGRLDATNIVEHDVSVITSLAVDHVDWLGDD
EEEEHHHHHHHHHHHHHHCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
INVIGFEKAGIYRSGKPAICGQPKPPATVAAHADDINAEFYQVGIQYTYDVDGDTWNWRS
CEEEEEECCCCEECCCCCCCCCCCCCCEEEECCCCCCCEEEEEEEEEEEECCCCCCCCCC
GAFQLESLPIPSLPLPNAATALMALGTSELSISDVNVVNGMKNAQLPGRMQQISEQPVIV
CCEEEECCCCCCCCCCCHHHHHHHCCCCCCEECCHHHHCCCCCCCCCCHHHHHCCCCEEE
LDVAHNPHSAEYFAQQVAKKYAGKNLHVVVAMLHDKDIPATLEVLAPITTHWYPASLKGP
EEECCCCCHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHCEECCCCCCC
RAATAAELCQSLPEGVEQYSNPVVAFEAALASVIDDDVVLVVGSFHTVGEVLEHWQKKGN
CHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3040739; 3040734; 9205837; 9278503; 1989505 [H]