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The map label for this gene is mnmC [H]

Identifier: 218708896

GI number: 218708896

Start: 920234

End: 922384

Strand: Reverse

Name: mnmC [H]

Synonym: VS_0897

Alternate gene names: 218708896

Gene position: 922384-920234 (Counterclockwise)

Preceding gene: 218708908

Following gene: 218708892

Centisome position: 27.96

GC content: 45.51

Gene sequence:

>2151_bases
GTGGTCAGCTTTCCTAGAAATTCGTACAATCCCTACCAACATATCGCTTCAAATCACAAAATGATAGATTTTATGACTTC
AATTACTAATGCAGAACTGGAATGGAATGAGTCTGGCACGCCAGTTTCAGACCAATTTGACGACGTTTACTTCTCTAATG
TTAACGGTTTAGAAGAAACTCGCTACGTCTTTTTAAAACAAAACCACCTTCCAGAGCGTTGGGTTGAACATCAACAACGC
CGTTTTGTGATTGCTGAAACCGGTTTTGGTACAGGTTTAAACTTTCTCGCAGTCTGGCAATGGTTCGATGCTTTTCTTAA
AGATAACCCACAAGCTATGACCAAAGAATTACATTTCATCAGTTTTGAAAAATATCCTTTAAATAAAGATGATCTGATCA
AAGCGCACCAATCTTGGCCTGAACTAGCCCAGTATGCGACACAACTCCAAGAACACTACCCGATTGCCCTGCCCGAATGT
CACCGCATTGTGTTGGGCGATGGTGCTATCACGCTTGATTTGTGGTTTGGTGATATTAAGGATTGTATGCCAAACGTGCC
GACTCCTCGCAAAGGTTTAGTCGATGCGTGGTTCTTAGATGGCTTTGCTCCAAGCAAAAACCCTGAAATGTGGAATCAAA
ACCTATTCAACGGCATGGCCAAACTGGCAAAACAAGATTGCAGCTGTGCAACGTTTACCGCTGCCGGTTTTGTTCGCCGC
GGTTTAATCGAAGCTGGCTTTGCGATGAAAAAGGTTAAAGGCTTTGGTACTAAGCGTGAAATGATTGCAGGCCGCCTTGA
CGAGAAGCTCGCTCATACCAACATCAAACCTTGGTATGGCCTAGCTCAACACAGTGATTCACAAGATATCGCCATTATCG
GTGGTGGTGTTGCCAGTGCTGCGCTTGCAAAAACGTTAAACCGACGTGGTAAAAACATCACCCTTTATTGTGAACACCAA
CAAGCCGCTGGAAATGCCTCTGGCAACAATCAAGGTGCCATTTATCCGCTACTCAGCGAAGCGACATCGAATGTTTCAAG
AATATTTGGTCCGGGCTTATTGTTTGCCCGCCAATTTATTAATCAAGCAGCACAATCAGTAAACTTCGATCACAACTGGT
GTGGCGTGAATATCTTGATGTGGGATGAAGGTTCAACCAAAAAGCTCAACCGCATGTTAGAAGGCAATTTCCACACAGAT
CTGATTCAGCGTTTAACGCCAGAGCAAGCCAACGAAAAGATTGGCTTACCAGTCGATAAAGAGAGTGTCTACTTTCCACT
GGGAGGTTGGTTAAGCCCTCTCCAACTGACTCAAGGTTTGATTGGCAGGTTAGAAGAGACCAACCAAGTGAGCGCGCACT
ATCAACATCAAGTAACGCAACTTGAATGGTTAGAAACAGAACAGCAATGGTTGCTGACGATTGAGACGCCTCAAGGTGAA
ATTCAAACCAAGCACGACCAAGTAGTTGTCGCGAATGGACACAAGTTCACCCAATTTGAGCAAACTCGCCCCGTACCTCT
CACGCCAGTTAAAGGGCAAGTGAGTCATATCCCGACTACAGAAAACCTCAGCCAGCTAAAAACGGTGTTGTGTTTTGATG
GTTACTTAACGCCACAGAATCCAAATAATGGTCACCACTGTATTGGCGCGAACTACGACAAGACCAATATCGACCAAGAG
TTTGATATTGAGGTTCAGCAACATAATGGTGAGCGCCTACATGGGTCGCTACCAGATCAAGCATGGACAAAAGATGTCGA
CACCAGTGACAATTTAGCGCGCCAAGGTATCCGAAGCGTAAGCCGTGACCACCTTCCGTTTGTCGGAAACGTTGGCGATT
TTGAATCAATCAAAGAACAGTACCAAGACCTGCACAACTTCAACCCGCAACGTCACTCTGTTGATGATATTCAAACGGTA
GCAAGCTTCCCTAACCTATACTGCTTTATCGGGCTAGGCTCACGTGGCTTGAGCTCTGCGCCATTATTAGCTGAAGTGTT
AGCATCACAAATCTGTGGCGATCCCCTACCCTTGCCTGTTGATGTGCTTGAGGCGATTCACCCAAGCAGGATGTGGGTGC
GCAGACTGCGTAAAGGTAAGGCATTAACGGCTGGATGGGTTGCAGACAAGCACTCTAAATCAAATTCATAG

Upstream 100 bases:

>100_bases
ATACTTGAAACAATACCCATACCGGTGATTACGACTCGTTTCATGTGACATTCCTATAATTCTAAATTCAGCTAGATGAT
AACTAAGAAGCCTAACAAAA

Downstream 100 bases:

>100_bases
TTTCTAGTTCAGCTATTCTGAAGTTTCTGAAGTTTCTGAAGTTTCTGAATATCAGAAATATCTAAGGCTAAAAGCTAGAA
AGCCCGAGCGACACTATGTC

Product: 5-methylaminomethyl-2-thiouridine methyltransferase

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase [H]

Number of amino acids: Translated: 716; Mature: 716

Protein sequence:

>716_residues
MVSFPRNSYNPYQHIASNHKMIDFMTSITNAELEWNESGTPVSDQFDDVYFSNVNGLEETRYVFLKQNHLPERWVEHQQR
RFVIAETGFGTGLNFLAVWQWFDAFLKDNPQAMTKELHFISFEKYPLNKDDLIKAHQSWPELAQYATQLQEHYPIALPEC
HRIVLGDGAITLDLWFGDIKDCMPNVPTPRKGLVDAWFLDGFAPSKNPEMWNQNLFNGMAKLAKQDCSCATFTAAGFVRR
GLIEAGFAMKKVKGFGTKREMIAGRLDEKLAHTNIKPWYGLAQHSDSQDIAIIGGGVASAALAKTLNRRGKNITLYCEHQ
QAAGNASGNNQGAIYPLLSEATSNVSRIFGPGLLFARQFINQAAQSVNFDHNWCGVNILMWDEGSTKKLNRMLEGNFHTD
LIQRLTPEQANEKIGLPVDKESVYFPLGGWLSPLQLTQGLIGRLEETNQVSAHYQHQVTQLEWLETEQQWLLTIETPQGE
IQTKHDQVVVANGHKFTQFEQTRPVPLTPVKGQVSHIPTTENLSQLKTVLCFDGYLTPQNPNNGHHCIGANYDKTNIDQE
FDIEVQQHNGERLHGSLPDQAWTKDVDTSDNLARQGIRSVSRDHLPFVGNVGDFESIKEQYQDLHNFNPQRHSVDDIQTV
ASFPNLYCFIGLGSRGLSSAPLLAEVLASQICGDPLPLPVDVLEAIHPSRMWVRRLRKGKALTAGWVADKHSKSNS

Sequences:

>Translated_716_residues
MVSFPRNSYNPYQHIASNHKMIDFMTSITNAELEWNESGTPVSDQFDDVYFSNVNGLEETRYVFLKQNHLPERWVEHQQR
RFVIAETGFGTGLNFLAVWQWFDAFLKDNPQAMTKELHFISFEKYPLNKDDLIKAHQSWPELAQYATQLQEHYPIALPEC
HRIVLGDGAITLDLWFGDIKDCMPNVPTPRKGLVDAWFLDGFAPSKNPEMWNQNLFNGMAKLAKQDCSCATFTAAGFVRR
GLIEAGFAMKKVKGFGTKREMIAGRLDEKLAHTNIKPWYGLAQHSDSQDIAIIGGGVASAALAKTLNRRGKNITLYCEHQ
QAAGNASGNNQGAIYPLLSEATSNVSRIFGPGLLFARQFINQAAQSVNFDHNWCGVNILMWDEGSTKKLNRMLEGNFHTD
LIQRLTPEQANEKIGLPVDKESVYFPLGGWLSPLQLTQGLIGRLEETNQVSAHYQHQVTQLEWLETEQQWLLTIETPQGE
IQTKHDQVVVANGHKFTQFEQTRPVPLTPVKGQVSHIPTTENLSQLKTVLCFDGYLTPQNPNNGHHCIGANYDKTNIDQE
FDIEVQQHNGERLHGSLPDQAWTKDVDTSDNLARQGIRSVSRDHLPFVGNVGDFESIKEQYQDLHNFNPQRHSVDDIQTV
ASFPNLYCFIGLGSRGLSSAPLLAEVLASQICGDPLPLPVDVLEAIHPSRMWVRRLRKGKALTAGWVADKHSKSNS
>Mature_716_residues
MVSFPRNSYNPYQHIASNHKMIDFMTSITNAELEWNESGTPVSDQFDDVYFSNVNGLEETRYVFLKQNHLPERWVEHQQR
RFVIAETGFGTGLNFLAVWQWFDAFLKDNPQAMTKELHFISFEKYPLNKDDLIKAHQSWPELAQYATQLQEHYPIALPEC
HRIVLGDGAITLDLWFGDIKDCMPNVPTPRKGLVDAWFLDGFAPSKNPEMWNQNLFNGMAKLAKQDCSCATFTAAGFVRR
GLIEAGFAMKKVKGFGTKREMIAGRLDEKLAHTNIKPWYGLAQHSDSQDIAIIGGGVASAALAKTLNRRGKNITLYCEHQ
QAAGNASGNNQGAIYPLLSEATSNVSRIFGPGLLFARQFINQAAQSVNFDHNWCGVNILMWDEGSTKKLNRMLEGNFHTD
LIQRLTPEQANEKIGLPVDKESVYFPLGGWLSPLQLTQGLIGRLEETNQVSAHYQHQVTQLEWLETEQQWLLTIETPQGE
IQTKHDQVVVANGHKFTQFEQTRPVPLTPVKGQVSHIPTTENLSQLKTVLCFDGYLTPQNPNNGHHCIGANYDKTNIDQE
FDIEVQQHNGERLHGSLPDQAWTKDVDTSDNLARQGIRSVSRDHLPFVGNVGDFESIKEQYQDLHNFNPQRHSVDDIQTV
ASFPNLYCFIGLGSRGLSSAPLLAEVLASQICGDPLPLPVDVLEAIHPSRMWVRRLRKGKALTAGWVADKHSKSNS

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family [H]

Homologues:

Organism=Escherichia coli, GI87082084, Length=679, Percent_Identity=49.3372606774669, Blast_Score=653, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008471
- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610 [H]

Pfam domain/function: PF01266 DAO; PF05430 DUF752 [H]

EC number: =2.1.1.61 [H]

Molecular weight: Translated: 80611; Mature: 80611

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSFPRNSYNPYQHIASNHKMIDFMTSITNAELEWNESGTPVSDQFDDVYFSNVNGLEET
CCCCCCCCCCHHHHHHCCCHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHCCCCCCCCHH
RYVFLKQNHLPERWVEHQQRRFVIAETGFGTGLNFLAVWQWFDAFLKDNPQAMTKELHFI
EEEEEECCCCCHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHEE
SFEKYPLNKDDLIKAHQSWPELAQYATQLQEHYPIALPECHRIVLGDGAITLDLWFGDIK
EEECCCCCHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCEEEEEEEECHHH
DCMPNVPTPRKGLVDAWFLDGFAPSKNPEMWNQNLFNGMAKLAKQDCSCATFTAAGFVRR
HHCCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
GLIEAGFAMKKVKGFGTKREMIAGRLDEKLAHTNIKPWYGLAQHSDSQDIAIIGGGVASA
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCEEEEECCHHHH
ALAKTLNRRGKNITLYCEHQQAAGNASGNNQGAIYPLLSEATSNVSRIFGPGLLFARQFI
HHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHCCHHHHHHHHH
NQAAQSVNFDHNWCGVNILMWDEGSTKKLNRMLEGNFHTDLIQRLTPEQANEKIGLPVDK
HHHHHHCCCCCCEEEEEEEEECCCCHHHHHHHHCCCHHHHHHHHCCCHHCCCCCCCCCCC
ESVYFPLGGWLSPLQLTQGLIGRLEETNQVSAHYQHQVTQLEWLETEQQWLLTIETPQGE
CCEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCC
IQTKHDQVVVANGHKFTQFEQTRPVPLTPVKGQVSHIPTTENLSQLKTVLCFDGYLTPQN
CCCCCCEEEEECCCEECHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEECCCC
PNNGHHCIGANYDKTNIDQEFDIEVQQHNGERLHGSLPDQAWTKDVDTSDNLARQGIRSV
CCCCCEEECCCCCCCCCCCEEEEEEEECCCCEECCCCCCHHHCCCCCCCHHHHHHHHHHH
SRDHLPFVGNVGDFESIKEQYQDLHNFNPQRHSVDDIQTVASFPNLYCFIGLGSRGLSSA
HHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCEEEEEEECCCCCCCC
PLLAEVLASQICGDPLPLPVDVLEAIHPSRMWVRRLRKGKALTAGWVADKHSKSNS
HHHHHHHHHHHCCCCCCCCHHHHHHCCHHHHHHHHHHCCCCEEECCCCCCCCCCCC
>Mature Secondary Structure
MVSFPRNSYNPYQHIASNHKMIDFMTSITNAELEWNESGTPVSDQFDDVYFSNVNGLEET
CCCCCCCCCCHHHHHHCCCHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHCCCCCCCCHH
RYVFLKQNHLPERWVEHQQRRFVIAETGFGTGLNFLAVWQWFDAFLKDNPQAMTKELHFI
EEEEEECCCCCHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHEE
SFEKYPLNKDDLIKAHQSWPELAQYATQLQEHYPIALPECHRIVLGDGAITLDLWFGDIK
EEECCCCCHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCEEEEEEEECHHH
DCMPNVPTPRKGLVDAWFLDGFAPSKNPEMWNQNLFNGMAKLAKQDCSCATFTAAGFVRR
HHCCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
GLIEAGFAMKKVKGFGTKREMIAGRLDEKLAHTNIKPWYGLAQHSDSQDIAIIGGGVASA
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCEEEEECCHHHH
ALAKTLNRRGKNITLYCEHQQAAGNASGNNQGAIYPLLSEATSNVSRIFGPGLLFARQFI
HHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHCCHHHHHHHHH
NQAAQSVNFDHNWCGVNILMWDEGSTKKLNRMLEGNFHTDLIQRLTPEQANEKIGLPVDK
HHHHHHCCCCCCEEEEEEEEECCCCHHHHHHHHCCCHHHHHHHHCCCHHCCCCCCCCCCC
ESVYFPLGGWLSPLQLTQGLIGRLEETNQVSAHYQHQVTQLEWLETEQQWLLTIETPQGE
CCEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCC
IQTKHDQVVVANGHKFTQFEQTRPVPLTPVKGQVSHIPTTENLSQLKTVLCFDGYLTPQN
CCCCCCEEEEECCCEECHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEECCCC
PNNGHHCIGANYDKTNIDQEFDIEVQQHNGERLHGSLPDQAWTKDVDTSDNLARQGIRSV
CCCCCEEECCCCCCCCCCCEEEEEEEECCCCEECCCCCCHHHCCCCCCCHHHHHHHHHHH
SRDHLPFVGNVGDFESIKEQYQDLHNFNPQRHSVDDIQTVASFPNLYCFIGLGSRGLSSA
HHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCEEEEEEECCCCCCCC
PLLAEVLASQICGDPLPLPVDVLEAIHPSRMWVRRLRKGKALTAGWVADKHSKSNS
HHHHHHHHHHHCCCCCCCCHHHHHHCCHHHHHHHHHHCCCCEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA