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The map label for this gene is aprN [H]

Identifier: 218708879

GI number: 218708879

Start: 903600

End: 904745

Strand: Direct

Name: aprN [H]

Synonym: VS_0879

Alternate gene names: 218708879

Gene position: 903600-904745 (Clockwise)

Preceding gene: 218708878

Following gene: 218708880

Centisome position: 27.39

GC content: 45.99

Gene sequence:

>1146_bases
ATGCCGGTAATGAGAGTCCAAACACTTGCAGGAGCAATGTCACTTGTTCTTCTTTCCTCTCCTGTATTTGCTGTTGTTAA
GCATGACTTGAGAGACAGTTATATTGTCGTTTTAAAAGAGAGCACATCGGTGTCACGAGCGAATGATATTGCACGTGAAG
TTGCCAATGGGCGCGGTGTGCTTGGTCATCAATATAGCCACGCTTTGAAAGGGTTTTCTCTTAAGATCCCTGAGCAAGCA
CTCAACGGTCTTAAAAATAGGTTCCCCGAAATTGATTACATTGAGCCTGATATCGTCATGCACGCTTTACCACGAGGGGG
CAAACCTAATCGAGGCGGAGGTGGTGATAGTGTTGAGCGAGCTGTGCAGCAGGTTCCTTGGGGTGTAACTAGAGTCAAAG
GTGGTACTGTTGATATGAGTAATTCCTCATCTGTTGCTTGGGTTATAGACTCGGGTATTGACAGTAACCACCCAGATTTA
AATGTAAACAAATCTCGAGGGGCAAACTTTACTCGCGGTAAGAAAAATAATACTAGCGATGGTAATGGTCACGGTACACA
TGTAGCAGGCACTATTGGTGCTTATAATGATGCTTTTGATGTCGTTGGCGTTGCCCCCGGTGTGCAGGTAATTCCAGTAC
GAGTGTTAGATAACAGTGGCAGTGGCTCAATGTCGGGCGTGATTGCCGGTGTTGATCATGTTGCACGCTATGGCGGCTCT
GGTGACTGTGCCAATATGAGCTTGGGAGGAGCAGGCTCTTCCACGGCCTTAGATAGCGCGATTAAAACAGCTGCTGCCCA
AGGGATATTTTTCTCAATCGCAGCAGGTAATTCAACTCAGGATACAAGTGGTTTTACGCCAGCTAGCACTAACGGTACAA
ACATCTTTACGATTTCAGCATTTGGTGAAACTAACGATAGCTTCGCTTACTTTTCCAATTATGGTTCTGATGTAGATTAC
GCTCAACCGGGAGTCGATGTTCTATCGACCAAAGCGGGCGGAGGAACGGTTTCTTACAATGGTACTTCAATGGCGGCTCC
TCACTTTTGCGGAGTTTTGCTAGTAACCGGTGGCTCATTCTCTTTAGATGGAGTTGTGAATAATGATCTCGATAGCTATC
CTGACCCTATTGCTGTGAGCAATTAA

Upstream 100 bases:

>100_bases
GATGGGGTGTTTTTGTATCAGGGCCGGATAATTTTGAGTTAGTGATAAAACCATCAGCAGCGTCTATGCTTTATAGGATG
TTCATTTTTTTGGAAAAAGG

Downstream 100 bases:

>100_bases
TTCGTTTAACAGTATTGATAGCTTAATAGGTATAACAATGCCGTTGTAGACAATAGAAAACGACCTAATGGGTCGTTTTC
TATTGTGAATAATCATCGAG

Product: putative serine protease

Products: NA

Alternate protein names: Nattokinase [H]

Number of amino acids: Translated: 381; Mature: 380

Protein sequence:

>381_residues
MPVMRVQTLAGAMSLVLLSSPVFAVVKHDLRDSYIVVLKESTSVSRANDIAREVANGRGVLGHQYSHALKGFSLKIPEQA
LNGLKNRFPEIDYIEPDIVMHALPRGGKPNRGGGGDSVERAVQQVPWGVTRVKGGTVDMSNSSSVAWVIDSGIDSNHPDL
NVNKSRGANFTRGKKNNTSDGNGHGTHVAGTIGAYNDAFDVVGVAPGVQVIPVRVLDNSGSGSMSGVIAGVDHVARYGGS
GDCANMSLGGAGSSTALDSAIKTAAAQGIFFSIAAGNSTQDTSGFTPASTNGTNIFTISAFGETNDSFAYFSNYGSDVDY
AQPGVDVLSTKAGGGTVSYNGTSMAAPHFCGVLLVTGGSFSLDGVVNNDLDSYPDPIAVSN

Sequences:

>Translated_381_residues
MPVMRVQTLAGAMSLVLLSSPVFAVVKHDLRDSYIVVLKESTSVSRANDIAREVANGRGVLGHQYSHALKGFSLKIPEQA
LNGLKNRFPEIDYIEPDIVMHALPRGGKPNRGGGGDSVERAVQQVPWGVTRVKGGTVDMSNSSSVAWVIDSGIDSNHPDL
NVNKSRGANFTRGKKNNTSDGNGHGTHVAGTIGAYNDAFDVVGVAPGVQVIPVRVLDNSGSGSMSGVIAGVDHVARYGGS
GDCANMSLGGAGSSTALDSAIKTAAAQGIFFSIAAGNSTQDTSGFTPASTNGTNIFTISAFGETNDSFAYFSNYGSDVDY
AQPGVDVLSTKAGGGTVSYNGTSMAAPHFCGVLLVTGGSFSLDGVVNNDLDSYPDPIAVSN
>Mature_380_residues
PVMRVQTLAGAMSLVLLSSPVFAVVKHDLRDSYIVVLKESTSVSRANDIAREVANGRGVLGHQYSHALKGFSLKIPEQAL
NGLKNRFPEIDYIEPDIVMHALPRGGKPNRGGGGDSVERAVQQVPWGVTRVKGGTVDMSNSSSVAWVIDSGIDSNHPDLN
VNKSRGANFTRGKKNNTSDGNGHGTHVAGTIGAYNDAFDVVGVAPGVQVIPVRVLDNSGSGSMSGVIAGVDHVARYGGSG
DCANMSLGGAGSSTALDSAIKTAAAQGIFFSIAAGNSTQDTSGFTPASTNGTNIFTISAFGETNDSFAYFSNYGSDVDYA
QPGVDVLSTKAGGGTVSYNGTSMAAPHFCGVLLVTGGSFSLDGVVNNDLDSYPDPIAVSN

Specific function: Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides. Subtilisin NAT also has fibrinolytic activity [H]

COG id: COG1404

COG function: function code O; Subtilisin-like serine proteases

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S8 family [H]

Homologues:

Organism=Homo sapiens, GI31317307, Length=343, Percent_Identity=26.530612244898, Blast_Score=94, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6320775, Length=300, Percent_Identity=35.3333333333333, Blast_Score=140, Evalue=4e-34,
Organism=Saccharomyces cerevisiae, GI6324576, Length=255, Percent_Identity=34.5098039215686, Blast_Score=127, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6319893, Length=224, Percent_Identity=36.6071428571429, Blast_Score=109, Evalue=6e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000209
- InterPro:   IPR022398
- InterPro:   IPR015500
- InterPro:   IPR009020
- InterPro:   IPR010259 [H]

Pfam domain/function: PF05922 Inhibitor_I9; PF00082 Peptidase_S8 [H]

EC number: =3.4.21.62 [H]

Molecular weight: Translated: 39239; Mature: 39108

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS00136 SUBTILASE_ASP ; PS00137 SUBTILASE_HIS ; PS00138 SUBTILASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVMRVQTLAGAMSLVLLSSPVFAVVKHDLRDSYIVVLKESTSVSRANDIAREVANGRGV
CCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCC
LGHQYSHALKGFSLKIPEQALNGLKNRFPEIDYIEPDIVMHALPRGGKPNRGGGGDSVER
CCCHHHHHHCCCEEECCHHHHHHHHHHCCCCCCCCHHHHHEECCCCCCCCCCCCCHHHHH
AVQQVPWGVTRVKGGTVDMSNSSSVAWVIDSGIDSNHPDLNVNKSRGANFTRGKKNNTSD
HHHHCCCCEEEECCCEEECCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GNGHGTHVAGTIGAYNDAFDVVGVAPGVQVIPVRVLDNSGSGSMSGVIAGVDHVARYGGS
CCCCCCEEEEECCCCCCCHHEEECCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHCCCC
GDCANMSLGGAGSSTALDSAIKTAAAQGIFFSIAAGNSTQDTSGFTPASTNGTNIFTISA
CCCCCEEECCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEE
FGETNDSFAYFSNYGSDVDYAQPGVDVLSTKAGGGTVSYNGTSMAAPHFCGVLLVTGGSF
CCCCCCCEEEECCCCCCCCCCCCCCCEEEECCCCCEEEECCCCCCCHHHCEEEEEECCCE
SLDGVVNNDLDSYPDPIAVSN
EECCEECCCCCCCCCCEECCC
>Mature Secondary Structure 
PVMRVQTLAGAMSLVLLSSPVFAVVKHDLRDSYIVVLKESTSVSRANDIAREVANGRGV
CCCHHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCC
LGHQYSHALKGFSLKIPEQALNGLKNRFPEIDYIEPDIVMHALPRGGKPNRGGGGDSVER
CCCHHHHHHCCCEEECCHHHHHHHHHHCCCCCCCCHHHHHEECCCCCCCCCCCCCHHHHH
AVQQVPWGVTRVKGGTVDMSNSSSVAWVIDSGIDSNHPDLNVNKSRGANFTRGKKNNTSD
HHHHCCCCEEEECCCEEECCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GNGHGTHVAGTIGAYNDAFDVVGVAPGVQVIPVRVLDNSGSGSMSGVIAGVDHVARYGGS
CCCCCCEEEEECCCCCCCHHEEECCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHCCCC
GDCANMSLGGAGSSTALDSAIKTAAAQGIFFSIAAGNSTQDTSGFTPASTNGTNIFTISA
CCCCCEEECCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEE
FGETNDSFAYFSNYGSDVDYAQPGVDVLSTKAGGGTVSYNGTSMAAPHFCGVLLVTGGSF
CCCCCCCEEEECCCCCCCCCCCCCCCEEEECCCCCEEEECCCCCCCHHHCEEEEEECCCE
SLDGVVNNDLDSYPDPIAVSN
EECCEECCCCCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1369081 [H]