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The map label for this gene is grpE

Identifier: 218708670

GI number: 218708670

Start: 674246

End: 674827

Strand: Direct

Name: grpE

Synonym: VS_0649

Alternate gene names: 218708670

Gene position: 674246-674827 (Clockwise)

Preceding gene: 218708665

Following gene: 218708671

Centisome position: 20.44

GC content: 43.47

Gene sequence:

>582_bases
ATGAGCAACGAAGAAAACAAAGTAACGGAAGAAGAGCTAGATCAAATCATTGCTGAAGCTGAGAAAGTTGAAGAAGCTGA
GCTAAATGAAGAGTCTGTTGATGAGCAAGAAGCAAAAATCGCTCAACTAGAAGCTGCACTACTTTCTAGCGAATCTAAAG
TGAAAGAGCAGCAAGATTCTGTTCTTCGCGCAAAAGCTGAAGTTGAAAACATGCGTCGCCGTAGCGAGCAAGAAATTGAT
AAGGCGCGTAAATTCGCTTTGAACAAGTTTGCTGAAGGTCTACTTCCTGTCATCGACAACCTAGAGCGTGCAATGCAAGC
GGCAGATGCTGAAAACGAAGTGGTTAAGCCACTGTTTGAAGGTGTTGAGCTAACGCACAAAACGTTCGTAGACACAGTTG
CTAAGTTTGGTCTTAAAGAGATCAACCCTGAAGGTGAAGTGTTCAACCCTGAATTCCACCAAGCGATGTCTATCCAAGAA
AGTCCAGATCACGAATCAAACACGGTTATGTTCGTAATGCAAAAAGGTTACGAGTTAAACGGTCGTGTGGTTCGCCCTGC
GATGGTTATGGTTGCTAAGTAA

Upstream 100 bases:

>100_bases
CCATAATAAAGGCAAGTTGAACGTCTATGCGAGTGTTTTATATCAATTTGGGGTGCAAACACACCTGTCACTTGCATTGA
AAACGAATTCTGGAGAGATC

Downstream 100 bases:

>100_bases
TGACGTGTTACGCTGCCAAGTGATTGAGTCGCGCATTGACTGAGTAAGTCGCGGCGACAAAATAAATCACTGGTTTACGT
AAAATGAAAAGAGAGGCATA

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 193; Mature: 192

Protein sequence:

>193_residues
MSNEENKVTEEELDQIIAEAEKVEEAELNEESVDEQEAKIAQLEAALLSSESKVKEQQDSVLRAKAEVENMRRRSEQEID
KARKFALNKFAEGLLPVIDNLERAMQAADAENEVVKPLFEGVELTHKTFVDTVAKFGLKEINPEGEVFNPEFHQAMSIQE
SPDHESNTVMFVMQKGYELNGRVVRPAMVMVAK

Sequences:

>Translated_193_residues
MSNEENKVTEEELDQIIAEAEKVEEAELNEESVDEQEAKIAQLEAALLSSESKVKEQQDSVLRAKAEVENMRRRSEQEID
KARKFALNKFAEGLLPVIDNLERAMQAADAENEVVKPLFEGVELTHKTFVDTVAKFGLKEINPEGEVFNPEFHQAMSIQE
SPDHESNTVMFVMQKGYELNGRVVRPAMVMVAK
>Mature_192_residues
SNEENKVTEEELDQIIAEAEKVEEAELNEESVDEQEAKIAQLEAALLSSESKVKEQQDSVLRAKAEVENMRRRSEQEIDK
ARKFALNKFAEGLLPVIDNLERAMQAADAENEVVKPLFEGVELTHKTFVDTVAKFGLKEINPEGEVFNPEFHQAMSIQES
PDHESNTVMFVMQKGYELNGRVVRPAMVMVAK

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=168, Percent_Identity=35.1190476190476, Blast_Score=92, Evalue=2e-19,
Organism=Homo sapiens, GI40255109, Length=148, Percent_Identity=31.7567567567568, Blast_Score=76, Evalue=2e-14,
Organism=Escherichia coli, GI1788967, Length=197, Percent_Identity=44.6700507614213, Blast_Score=155, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17552458, Length=141, Percent_Identity=34.7517730496454, Blast_Score=80, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6324806, Length=183, Percent_Identity=34.9726775956284, Blast_Score=115, Evalue=5e-27,
Organism=Drosophila melanogaster, GI24653432, Length=190, Percent_Identity=30, Blast_Score=92, Evalue=3e-19,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_VIBSL (B7VJW7)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002416291.1
- ProteinModelPortal:   B7VJW7
- SMR:   B7VJW7
- GeneID:   7160268
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_0649
- HOGENOM:   HBG732630
- OMA:   NLHQAVM
- ProtClustDB:   PRK14150
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 21822; Mature: 21691

Theoretical pI: Translated: 4.28; Mature: 4.28

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNEENKVTEEELDQIIAEAEKVEEAELNEESVDEQEAKIAQLEAALLSSESKVKEQQDS
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLRAKAEVENMRRRSEQEIDKARKFALNKFAEGLLPVIDNLERAMQAADAENEVVKPLFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
GVELTHKTFVDTVAKFGLKEINPEGEVFNPEFHQAMSIQESPDHESNTVMFVMQKGYELN
HHHHHHHHHHHHHHHHHHHHCCCCCCEECHHHHHHHCCCCCCCCCCCEEEEEEECCCCCC
GRVVRPAMVMVAK
CEEECHHHHHCCC
>Mature Secondary Structure 
SNEENKVTEEELDQIIAEAEKVEEAELNEESVDEQEAKIAQLEAALLSSESKVKEQQDS
CCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLRAKAEVENMRRRSEQEIDKARKFALNKFAEGLLPVIDNLERAMQAADAENEVVKPLFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
GVELTHKTFVDTVAKFGLKEINPEGEVFNPEFHQAMSIQESPDHESNTVMFVMQKGYELN
HHHHHHHHHHHHHHHHHHHHCCCCCCEECHHHHHHHCCCCCCCCCCCEEEEEEECCCCCC
GRVVRPAMVMVAK
CEEECHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA