Click here to switch to the map view.

The map label for this gene is alaS [C]

Identifier: 218708646

GI number: 218708646

Start: 646846

End: 647748

Strand: Direct

Name: alaS [C]

Synonym: VS_0624

Alternate gene names: 218708646

Gene position: 646846-647748 (Clockwise)

Preceding gene: 218708645

Following gene: 218708647

Centisome position: 19.61

GC content: 45.07

Gene sequence:

>903_bases
ATGACCACTAGCGATTTGATTACACCAGCGTCAGCAATCACACCAACCATCACTCAATTTTGTCATCAAACTTGGCTGCT
CAATGCAAAAGCGGTGTACGTTGAAAGTGACGACAGCAAAACTTACTTGATCACTGACGTGACACCTTTCCACCCTGTGA
GTCATATTTGGCCCGATCACCCAGCAGACCGAGGTTTTGTCAGCGTAGGTGGCGAACAGTATATTGTTGAAGATTGCCTT
GTTGGTGCAATAGAGCAATCTACTGGAAATCTTTATATTGCAGCAGATATCCCTGTTAAGCGTGATACGGCAGGGTGGGC
GTTTGTGGTTGTCCACCAACTACCTGTATCAGCTTCTATTATTAAAATTGGCGATGAAGTTGAGTTGTCGGTTGATAAAG
AGCACCAAGCCAGTTTGAGCCGCGGTCATAGTGCCGGACATATTGCCTTCTTAGCCTTGAATAAAGTATTGGCTGAGAGT
TACTGGCGTAAAGATGCGGATAGAAAAGATCCACTCGGCAGCTATGACTTCAATAGCTACGCACAGGTGACTAGCTTTGT
GACTCCAGAGTTGTGTACCGATAAGTATCGTTTAGGTAAAACATTGAAGAAACGCGGTCTGAACGTTGCTGACATGCTAA
CGAACCTTGATGGCATTGAGGCCAATATCAACCAGATGATTGCAGGCTGGCTTGCTGAATCGACTCTTGTAACGATGAGA
CTAGAAGGTGAGTCGTTAACAGACTCTCGCTACTGGGAATGGCAACTAGATGACGAGACTTTAGTGTCTATTCCGTGTGG
TGGTACTCACATCGACAATACTTCAGAACTTAAGGCATTGTCGGTTAAGTTAACCCAATTAGATGACCAACATATCGAAA
TGCAGACGTGTGTCTCTCGATGA

Upstream 100 bases:

>100_bases
CTCGGGTATTTTTTTAAGTAAAATTCACGTTATCTGATAATCGCGTCTTTGATTCCGTTATCAGCTAGCGACTTAGACTA
GATTCCAAAAGAAGAATGAT

Downstream 100 bases:

>100_bases
TCTCAATTAAAGATCGAAAATAGATTATTCTGCATTGTTGGTTTTATTTGTTTGTTTCTAGTCATATATTCCATTGTAAA
TTGAAATGCAGTATATGTAA

Product: putative metal-dependent hydrolase

Products: AMP; diphosphate; L-alanyl-tRNAAla [C]

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 299

Protein sequence:

>300_residues
MTTSDLITPASAITPTITQFCHQTWLLNAKAVYVESDDSKTYLITDVTPFHPVSHIWPDHPADRGFVSVGGEQYIVEDCL
VGAIEQSTGNLYIAADIPVKRDTAGWAFVVVHQLPVSASIIKIGDEVELSVDKEHQASLSRGHSAGHIAFLALNKVLAES
YWRKDADRKDPLGSYDFNSYAQVTSFVTPELCTDKYRLGKTLKKRGLNVADMLTNLDGIEANINQMIAGWLAESTLVTMR
LEGESLTDSRYWEWQLDDETLVSIPCGGTHIDNTSELKALSVKLTQLDDQHIEMQTCVSR

Sequences:

>Translated_300_residues
MTTSDLITPASAITPTITQFCHQTWLLNAKAVYVESDDSKTYLITDVTPFHPVSHIWPDHPADRGFVSVGGEQYIVEDCL
VGAIEQSTGNLYIAADIPVKRDTAGWAFVVVHQLPVSASIIKIGDEVELSVDKEHQASLSRGHSAGHIAFLALNKVLAES
YWRKDADRKDPLGSYDFNSYAQVTSFVTPELCTDKYRLGKTLKKRGLNVADMLTNLDGIEANINQMIAGWLAESTLVTMR
LEGESLTDSRYWEWQLDDETLVSIPCGGTHIDNTSELKALSVKLTQLDDQHIEMQTCVSR
>Mature_299_residues
TTSDLITPASAITPTITQFCHQTWLLNAKAVYVESDDSKTYLITDVTPFHPVSHIWPDHPADRGFVSVGGEQYIVEDCLV
GAIEQSTGNLYIAADIPVKRDTAGWAFVVVHQLPVSASIIKIGDEVELSVDKEHQASLSRGHSAGHIAFLALNKVLAESY
WRKDADRKDPLGSYDFNSYAQVTSFVTPELCTDKYRLGKTLKKRGLNVADMLTNLDGIEANINQMIAGWLAESTLVTMRL
EGESLTDSRYWEWQLDDETLVSIPCGGTHIDNTSELKALSVKLTQLDDQHIEMQTCVSR

Specific function: Unknown

COG id: COG2872

COG function: function code R; Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.1.1.7 [C]

Molecular weight: Translated: 33244; Mature: 33113

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTSDLITPASAITPTITQFCHQTWLLNAKAVYVESDDSKTYLITDVTPFHPVSHIWPDH
CCCCCCCCCHHHCCHHHHHHHHHHHEECCEEEEEECCCCCEEEEECCCCCCCHHHCCCCC
PADRGFVSVGGEQYIVEDCLVGAIEQSTGNLYIAADIPVKRDTAGWAFVVVHQLPVSASI
CCCCCEEEECCCCEEHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEEEEEEECCCCEEE
IKIGDEVELSVDKEHQASLSRGHSAGHIAFLALNKVLAESYWRKDADRKDPLGSYDFNSY
EEECCEEEEEECCHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCH
AQVTSFVTPELCTDKYRLGKTLKKRGLNVADMLTNLDGIEANINQMIAGWLAESTLVTMR
HHHHHHCCHHHHCCHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEEE
LEGESLTDSRYWEWQLDDETLVSIPCGGTHIDNTSELKALSVKLTQLDDQHIEMQTCVSR
ECCCCCCCCCEEEEEECCCEEEEECCCCCCCCCCCHHEEEEEEEECCCCHHHHHHHHHCC
>Mature Secondary Structure 
TTSDLITPASAITPTITQFCHQTWLLNAKAVYVESDDSKTYLITDVTPFHPVSHIWPDH
CCCCCCCCHHHCCHHHHHHHHHHHEECCEEEEEECCCCCEEEEECCCCCCCHHHCCCCC
PADRGFVSVGGEQYIVEDCLVGAIEQSTGNLYIAADIPVKRDTAGWAFVVVHQLPVSASI
CCCCCEEEECCCCEEHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEEEEEEECCCCEEE
IKIGDEVELSVDKEHQASLSRGHSAGHIAFLALNKVLAESYWRKDADRKDPLGSYDFNSY
EEECCEEEEEECCHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCH
AQVTSFVTPELCTDKYRLGKTLKKRGLNVADMLTNLDGIEANINQMIAGWLAESTLVTMR
HHHHHHCCHHHHCCHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEEE
LEGESLTDSRYWEWQLDDETLVSIPCGGTHIDNTSELKALSVKLTQLDDQHIEMQTCVSR
ECCCCCCCCCEEEEEECCCEEEEECCCCCCCCCCCHHEEEEEEEECCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; @ALAN01.txt*L-alanine!; tRNAAla [C]

Specific reaction: ATP + @ALAN01.txt*L-alanine! + tRNAAla = AMP + diphosphate + L-alanyl-tRNAAla [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA