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The map label for this gene is slt [H]

Identifier: 218708586

GI number: 218708586

Start: 575582

End: 577531

Strand: Direct

Name: slt [H]

Synonym: VS_0552

Alternate gene names: 218708586

Gene position: 575582-577531 (Clockwise)

Preceding gene: 218708582

Following gene: 218708587

Centisome position: 17.45

GC content: 44.1

Gene sequence:

>1950_bases
ATGTTTTTCCGCATTGGTAATACGAAAATTGCTGTCGCTGCATCGGCAATTTTGTTTTCATTTTCACAGGCTCCAATGGC
TATGGCCAGCAATGCTTCCGAGCTTGAAATGCAGCGTGATGTTTATGACAGAGCGCAAGAGGTTTTAGACAACCGTGATC
TAAAAGCTTATTCAGCACTTCGTAATAAAATTCAAACATACCCGTTAACACCTTACACTGACTATCGCGCCTTTTTATTG
GGCTTAGGTGATCGAACGCCAAGCGAGGTGGATGCTTTTATTGAAGAGAACAAAGCACTGCCGTTTTCCAATCGTATACG
AGCGCCTTATTTAGATTTATTGGCGGCTCAAAAACAGTGGAAGACGATCCTTGAGTTTCAAACCCAAGAGCCTGTTGGTG
AAAAATATCAATGTATCTATTACCGCGCTAATTATGAACAAGGTAATCAAGATCTCGCCTTTAAAGGTGCGAAACAACTT
TGGTTAAGTGGCAGTGGTGTTGATGATAACTGTGATTCACTTTTCGAAAGTTGGGATGAAGCGGGCTTAAGAACGGATCA
GCTGATTCTGGACAGAATGCTTTTAGCGTTCGAGAGTCGAAACGGTAAGTTAATGAGCTACTTGGTTAAGCAATTAGATC
ATGATGAGTCAATTACTCAAGCGAAGCAGATGAAGGCGTTGTACAACAAGCCTGAAGATGTACTCGCGTTTGCTAAGAAG
CACCCAGCGAATGAATTCTACCAAGCACAAACCGAATTTGGTTTCGAGAAGCTAGTAAGGAAATCCGCAAGCAGCGCTCA
AGAGGTTTTTGATGATGTTGTGAAAGCTCAAAAATTTTCGAAAGAAAAATCCCAAGAGCTCGCGGATTACCTAGCGTTCC
GCTTGATCAATACCGACTCTGAAGAGTTGATGGCGTGGCGAGACAAAATGCTCGCGAGTTCATCAAAGCAAGTTTTGCTG
GAAAGACGTGCTCGCTTAGCTATTCAACATGCCGATTGGACGGGTTTGAAAGAGTGGATTGCGCGCTTAGATGGTAAACA
TCAAGCTTCGCTTCGCTGGCAGTATTGGCAGGGTAGAGCTGAGATTGCGCTAGGCGATACTGCTGAAGGTAATAAGCGAT
TGTCAGACATTTTGGGTCAGCGTAATTTCTATAGTGTGGCTGCCGCTAAGCAATTAAATAAGCCTGTTAGCTATCCAACG
TCGACCCTTAAATACAGTGCAGAAATGGTGAAACCATTCGACACTTCTTTGGTTCGCATAGGTGAGCTGATTGAGCGAGA
TAAAATAGCGGCTGCAAAGAGTGAGTGGCGCTGGTTATTAAGTCATGCAGATAAAGAGCAAAAAGCGATGCTTGCTGCTC
ATGCTGCGACACAGCGATGGAATCACTTTACGGTAACGGCGAGTATTTCTGCGAAGATGTGGGATAACATTGCGTTACGT
TTCCCTGTCGCTCATAAGTGGTGGTTTAATTTCTATGCCGAGAAGCACGATCTCGATCCAATTACTTTGATGTCTTTAGC
AAGACAAGAGAGTGCGATGGATTCAGAGGCTCGCTCTCCAGTTGGCGCGCGTGGCATCATGCAAATTATGCCAAAGACGG
CGCAATACACGGCTAACAAACATAAGATTAAGTACCAGGGCAGCGATGATCTTTATGATGTAGGTAAGAATATTGAGATA
GGCAGTCATTATTTAGATGGCCTACTCGCTCAATATGATGATAACCGCATCTTCGCATTTGCCGCGTATAATGCTGGCCC
TAGTCGTGTGAAACAGTGGCGATCTCGCAGCGATGAAAAGCTCGATGCATTTGCTTTTATCGAGATGATCCCATTCAAAG
AGACTCGTGGTTACGTTCAGAATATCTTGATGTTTGAGACTTACTATCGAGATATCCTGGGTGAGAAAGGGACGTTTTTA
GCACCTCACGAGGCAAAAACGAAATACTAA

Upstream 100 bases:

>100_bases
TTAAATATTAACAATATTTACACTCAAACTCTTTACATTTGATGTGATGTCGGGCGTAAATAGACTTCACGTATTCATAC
ACGCACTAAGGAAAGAGCGA

Downstream 100 bases:

>100_bases
GCTTTCTGTATTTTTCTCCAAGCTGAGTAATATTAAAGGCAGTGAGTGCGAACCATTTCCACTTACTGCCTTTTTACATT
GGATCACATCAAGTGTTTCG

Product: soluble lytic murein transglycosylase precursor

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 649; Mature: 649

Protein sequence:

>649_residues
MFFRIGNTKIAVAASAILFSFSQAPMAMASNASELEMQRDVYDRAQEVLDNRDLKAYSALRNKIQTYPLTPYTDYRAFLL
GLGDRTPSEVDAFIEENKALPFSNRIRAPYLDLLAAQKQWKTILEFQTQEPVGEKYQCIYYRANYEQGNQDLAFKGAKQL
WLSGSGVDDNCDSLFESWDEAGLRTDQLILDRMLLAFESRNGKLMSYLVKQLDHDESITQAKQMKALYNKPEDVLAFAKK
HPANEFYQAQTEFGFEKLVRKSASSAQEVFDDVVKAQKFSKEKSQELADYLAFRLINTDSEELMAWRDKMLASSSKQVLL
ERRARLAIQHADWTGLKEWIARLDGKHQASLRWQYWQGRAEIALGDTAEGNKRLSDILGQRNFYSVAAAKQLNKPVSYPT
STLKYSAEMVKPFDTSLVRIGELIERDKIAAAKSEWRWLLSHADKEQKAMLAAHAATQRWNHFTVTASISAKMWDNIALR
FPVAHKWWFNFYAEKHDLDPITLMSLARQESAMDSEARSPVGARGIMQIMPKTAQYTANKHKIKYQGSDDLYDVGKNIEI
GSHYLDGLLAQYDDNRIFAFAAYNAGPSRVKQWRSRSDEKLDAFAFIEMIPFKETRGYVQNILMFETYYRDILGEKGTFL
APHEAKTKY

Sequences:

>Translated_649_residues
MFFRIGNTKIAVAASAILFSFSQAPMAMASNASELEMQRDVYDRAQEVLDNRDLKAYSALRNKIQTYPLTPYTDYRAFLL
GLGDRTPSEVDAFIEENKALPFSNRIRAPYLDLLAAQKQWKTILEFQTQEPVGEKYQCIYYRANYEQGNQDLAFKGAKQL
WLSGSGVDDNCDSLFESWDEAGLRTDQLILDRMLLAFESRNGKLMSYLVKQLDHDESITQAKQMKALYNKPEDVLAFAKK
HPANEFYQAQTEFGFEKLVRKSASSAQEVFDDVVKAQKFSKEKSQELADYLAFRLINTDSEELMAWRDKMLASSSKQVLL
ERRARLAIQHADWTGLKEWIARLDGKHQASLRWQYWQGRAEIALGDTAEGNKRLSDILGQRNFYSVAAAKQLNKPVSYPT
STLKYSAEMVKPFDTSLVRIGELIERDKIAAAKSEWRWLLSHADKEQKAMLAAHAATQRWNHFTVTASISAKMWDNIALR
FPVAHKWWFNFYAEKHDLDPITLMSLARQESAMDSEARSPVGARGIMQIMPKTAQYTANKHKIKYQGSDDLYDVGKNIEI
GSHYLDGLLAQYDDNRIFAFAAYNAGPSRVKQWRSRSDEKLDAFAFIEMIPFKETRGYVQNILMFETYYRDILGEKGTFL
APHEAKTKY
>Mature_649_residues
MFFRIGNTKIAVAASAILFSFSQAPMAMASNASELEMQRDVYDRAQEVLDNRDLKAYSALRNKIQTYPLTPYTDYRAFLL
GLGDRTPSEVDAFIEENKALPFSNRIRAPYLDLLAAQKQWKTILEFQTQEPVGEKYQCIYYRANYEQGNQDLAFKGAKQL
WLSGSGVDDNCDSLFESWDEAGLRTDQLILDRMLLAFESRNGKLMSYLVKQLDHDESITQAKQMKALYNKPEDVLAFAKK
HPANEFYQAQTEFGFEKLVRKSASSAQEVFDDVVKAQKFSKEKSQELADYLAFRLINTDSEELMAWRDKMLASSSKQVLL
ERRARLAIQHADWTGLKEWIARLDGKHQASLRWQYWQGRAEIALGDTAEGNKRLSDILGQRNFYSVAAAKQLNKPVSYPT
STLKYSAEMVKPFDTSLVRIGELIERDKIAAAKSEWRWLLSHADKEQKAMLAAHAATQRWNHFTVTASISAKMWDNIALR
FPVAHKWWFNFYAEKHDLDPITLMSLARQESAMDSEARSPVGARGIMQIMPKTAQYTANKHKIKYQGSDDLYDVGKNIEI
GSHYLDGLLAQYDDNRIFAFAAYNAGPSRVKQWRSRSDEKLDAFAFIEMIPFKETRGYVQNILMFETYYRDILGEKGTFL
APHEAKTKY

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=640, Percent_Identity=33.4375, Blast_Score=384, Evalue=1e-107,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 74495; Mature: 74495

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFFRIGNTKIAVAASAILFSFSQAPMAMASNASELEMQRDVYDRAQEVLDNRDLKAYSAL
CEEEECCCCHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHH
RNKIQTYPLTPYTDYRAFLLGLGDRTPSEVDAFIEENKALPFSNRIRAPYLDLLAAQKQW
HHHHCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
KTILEFQTQEPVGEKYQCIYYRANYEQGNQDLAFKGAKQLWLSGSGVDDNCDSLFESWDE
HHHHHHHCCCCCCCCEEEEEEEECCCCCCCCHHHCCCHHHEECCCCCCCCHHHHHHHHHH
AGLRTDQLILDRMLLAFESRNGKLMSYLVKQLDHDESITQAKQMKALYNKPEDVLAFAKK
CCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHH
HPANEFYQAQTEFGFEKLVRKSASSAQEVFDDVVKAQKFSKEKSQELADYLAFRLINTDS
CCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
EELMAWRDKMLASSSKQVLLERRARLAIQHADWTGLKEWIARLDGKHQASLRWQYWQGRA
HHHHHHHHHHHHCCHHHHHHHHHHHHEEECCCCCCHHHHHHHCCCCCCCCEEEEEECCCE
EIALGDTAEGNKRLSDILGQRNFYSVAAAKQLNKPVSYPTSTLKYSAEMVKPFDTSLVRI
EEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHH
GELIERDKIAAAKSEWRWLLSHADKEQKAMLAAHAATQRWNHFTVTASISAKMWDNIALR
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEEEEECHHHHCCEEEE
FPVAHKWWFNFYAEKHDLDPITLMSLARQESAMDSEARSPVGARGIMQIMPKTAQYTANK
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHCCCHHHHCCCC
HKIKYQGSDDLYDVGKNIEIGSHYLDGLLAQYDDNRIFAFAAYNAGPSRVKQWRSRSDEK
CEEEECCCCCHHHCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHCCCCCH
LDAFAFIEMIPFKETRGYVQNILMFETYYRDILGEKGTFLAPHEAKTKY
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCC
>Mature Secondary Structure
MFFRIGNTKIAVAASAILFSFSQAPMAMASNASELEMQRDVYDRAQEVLDNRDLKAYSAL
CEEEECCCCHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHH
RNKIQTYPLTPYTDYRAFLLGLGDRTPSEVDAFIEENKALPFSNRIRAPYLDLLAAQKQW
HHHHCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
KTILEFQTQEPVGEKYQCIYYRANYEQGNQDLAFKGAKQLWLSGSGVDDNCDSLFESWDE
HHHHHHHCCCCCCCCEEEEEEEECCCCCCCCHHHCCCHHHEECCCCCCCCHHHHHHHHHH
AGLRTDQLILDRMLLAFESRNGKLMSYLVKQLDHDESITQAKQMKALYNKPEDVLAFAKK
CCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHH
HPANEFYQAQTEFGFEKLVRKSASSAQEVFDDVVKAQKFSKEKSQELADYLAFRLINTDS
CCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
EELMAWRDKMLASSSKQVLLERRARLAIQHADWTGLKEWIARLDGKHQASLRWQYWQGRA
HHHHHHHHHHHHCCHHHHHHHHHHHHEEECCCCCCHHHHHHHCCCCCCCCEEEEEECCCE
EIALGDTAEGNKRLSDILGQRNFYSVAAAKQLNKPVSYPTSTLKYSAEMVKPFDTSLVRI
EEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHH
GELIERDKIAAAKSEWRWLLSHADKEQKAMLAAHAATQRWNHFTVTASISAKMWDNIALR
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEEEEECHHHHCCEEEE
FPVAHKWWFNFYAEKHDLDPITLMSLARQESAMDSEARSPVGARGIMQIMPKTAQYTANK
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHCCCHHHHCCCC
HKIKYQGSDDLYDVGKNIEIGSHYLDGLLAQYDDNRIFAFAAYNAGPSRVKQWRSRSDEK
CEEEECCCCCHHHCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHCCCCCH
LDAFAFIEMIPFKETRGYVQNILMFETYYRDILGEKGTFLAPHEAKTKY
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]