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The map label for this gene is ispH [H]

Identifier: 218708573

GI number: 218708573

Start: 560086

End: 561042

Strand: Direct

Name: ispH [H]

Synonym: VS_0539

Alternate gene names: 218708573

Gene position: 560086-561042 (Clockwise)

Preceding gene: 218708572

Following gene: 218708577

Centisome position: 16.98

GC content: 46.92

Gene sequence:

>957_bases
ATGAGCAATGAAATGAAAATAATGTTAGCTAACCCTCGTGGCTTTTGTGCCGGTGTCGATCGTGCGATCAGTATTGTTGA
GCGTGCACTCGAAATGTACCAGCCACCGATCTATGTTCGTCATGAAGTGGTACACAACCGCTTTGTTGTTGAAGGGCTAA
AGCAACGTGGTGCTATTTTTGTCGAAGAGTTAAGTGAAGTGCCAGACGATAACATCGTGATCTTCTCGGCTCACGGTGTT
TCTCAAGCGGTTCGTAAAGAAGCGAAAGAACGCGAGTTAACGGTATTTGATGCGACGTGTCCTTTGGTGACTAAAGTTCA
TATGGAGGTTGCTCGAGCGAGCCGCAAACATATGGAAGTGGTTCTGATTGGTCACGCGGGTCACCCTGAAGTGGAAGGCA
CTATGGGTCAGTACGCTAGCCAAACTGGCGGTATGTACTTGGTTGAAAGACCAGAAGATGTACAGAACTTAGTCGTGAAC
GACCCGAGTAATTTACATTACGTCAGCCAAACCACGTTGTCAGTTGATGAAACCGCAGATGTGATTGAAGAGCTGCGTCG
AGTGTTCCCTGAGATCCAAGGTCCACGTAAAGACGACATCTGCTACGCGACTCAAAACCGTCAGGACGCTATTCGTGAGA
TGGCCGGTGATGTTGATGTCGTGATAGTTGTTGGTTCTAAGAACTCATCGAACTCAACTCGTTTAAAAGAGCTGGCTGAG
AAGCTAGGCACGCCAGGTTATCTAACCGACTGTCCTGAAGACATTCAAACTGAATGGGTTGAAGGGAAAAAGAAAATTGG
TGTGACAGCGGGTGCTTCAGCGCCTGAAGAGCTAGTAAACCAAATCTTAGATCGTATTCGTGAGTTGGGTGCTACCGAGG
TTGAAGAGATTCAAGGTCGTGAAGAGAATATGTTCTTCGAAGTACCAAAAGAGCTGCAGATTAAGCAAGTCGACTAG

Upstream 100 bases:

>100_bases
TGATGTAACCCAGAGCTTCACTGTCTAGACGCTTCACTGTAGAATGCGAGCCTCGCTGACAGCGAACTCTATAGAAACAA
CCTTAGTACCGCGGTAAATG

Downstream 100 bases:

>100_bases
TCTCTATGGATTGGTGACAAGATCGATTCGTAACTCTATCGATTCGCAAACAGTATTGATTTGTAAACAGCAGGAAGCCA
ACGTTGATGCGTTGGCTTTT

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MSNEMKIMLANPRGFCAGVDRAISIVERALEMYQPPIYVRHEVVHNRFVVEGLKQRGAIFVEELSEVPDDNIVIFSAHGV
SQAVRKEAKERELTVFDATCPLVTKVHMEVARASRKHMEVVLIGHAGHPEVEGTMGQYASQTGGMYLVERPEDVQNLVVN
DPSNLHYVSQTTLSVDETADVIEELRRVFPEIQGPRKDDICYATQNRQDAIREMAGDVDVVIVVGSKNSSNSTRLKELAE
KLGTPGYLTDCPEDIQTEWVEGKKKIGVTAGASAPEELVNQILDRIRELGATEVEEIQGREENMFFEVPKELQIKQVD

Sequences:

>Translated_318_residues
MSNEMKIMLANPRGFCAGVDRAISIVERALEMYQPPIYVRHEVVHNRFVVEGLKQRGAIFVEELSEVPDDNIVIFSAHGV
SQAVRKEAKERELTVFDATCPLVTKVHMEVARASRKHMEVVLIGHAGHPEVEGTMGQYASQTGGMYLVERPEDVQNLVVN
DPSNLHYVSQTTLSVDETADVIEELRRVFPEIQGPRKDDICYATQNRQDAIREMAGDVDVVIVVGSKNSSNSTRLKELAE
KLGTPGYLTDCPEDIQTEWVEGKKKIGVTAGASAPEELVNQILDRIRELGATEVEEIQGREENMFFEVPKELQIKQVD
>Mature_317_residues
SNEMKIMLANPRGFCAGVDRAISIVERALEMYQPPIYVRHEVVHNRFVVEGLKQRGAIFVEELSEVPDDNIVIFSAHGVS
QAVRKEAKERELTVFDATCPLVTKVHMEVARASRKHMEVVLIGHAGHPEVEGTMGQYASQTGGMYLVERPEDVQNLVVND
PSNLHYVSQTTLSVDETADVIEELRRVFPEIQGPRKDDICYATQNRQDAIREMAGDVDVVIVVGSKNSSNSTRLKELAEK
LGTPGYLTDCPEDIQTEWVEGKKKIGVTAGASAPEELVNQILDRIRELGATEVEEIQGREENMFFEVPKELQIKQVD

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) [H]

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family [H]

Homologues:

Organism=Escherichia coli, GI1786212, Length=316, Percent_Identity=66.7721518987342, Blast_Score=439, Evalue=1e-124,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003451 [H]

Pfam domain/function: PF02401 LYTB [H]

EC number: =1.17.1.2 [H]

Molecular weight: Translated: 35542; Mature: 35411

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNEMKIMLANPRGFCAGVDRAISIVERALEMYQPPIYVRHEVVHNRFVVEGLKQRGAIF
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCCCH
VEELSEVPDDNIVIFSAHGVSQAVRKEAKERELTVFDATCPLVTKVHMEVARASRKHMEV
HHHHHHCCCCCEEEEECCCHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHCCEEE
VLIGHAGHPEVEGTMGQYASQTGGMYLVERPEDVQNLVVNDPSNLHYVSQTTLSVDETAD
EEEECCCCCCCCCHHHHHHHCCCCEEEEECCHHHHHHCCCCCCCEEEEEECEECHHHHHH
VIEELRRVFPEIQGPRKDDICYATQNRQDAIREMAGDVDVVIVVGSKNSSNSTRLKELAE
HHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHH
KLGTPGYLTDCPEDIQTEWVEGKKKIGVTAGASAPEELVNQILDRIRELGATEVEEIQGR
HHCCCCCCCCCCHHHHHHHHCCHHHCCEECCCCCHHHHHHHHHHHHHHCCCCHHHHHCCC
EENMFFEVPKELQIKQVD
CCCEEEECCCCCCEEECC
>Mature Secondary Structure 
SNEMKIMLANPRGFCAGVDRAISIVERALEMYQPPIYVRHEVVHNRFVVEGLKQRGAIF
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCCCH
VEELSEVPDDNIVIFSAHGVSQAVRKEAKERELTVFDATCPLVTKVHMEVARASRKHMEV
HHHHHHCCCCCEEEEECCCHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHCCEEE
VLIGHAGHPEVEGTMGQYASQTGGMYLVERPEDVQNLVVNDPSNLHYVSQTTLSVDETAD
EEEECCCCCCCCCHHHHHHHCCCCEEEEECCHHHHHHCCCCCCCEEEEEECEECHHHHHH
VIEELRRVFPEIQGPRKDDICYATQNRQDAIREMAGDVDVVIVVGSKNSSNSTRLKELAE
HHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHH
KLGTPGYLTDCPEDIQTEWVEGKKKIGVTAGASAPEELVNQILDRIRELGATEVEEIQGR
HHCCCCCCCCCCHHHHHHHHCCHHHCCEECCCCCHHHHHHHHHHHHHHCCCCHHHHHCCC
EENMFFEVPKELQIKQVD
CCCEEEECCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]