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The map label for this gene is thyA

Identifier: 218708561

GI number: 218708561

Start: 546137

End: 546988

Strand: Direct

Name: thyA

Synonym: VS_0527

Alternate gene names: 218708561

Gene position: 546137-546988 (Clockwise)

Preceding gene: 218708560

Following gene: 218708562

Centisome position: 16.55

GC content: 45.89

Gene sequence:

>852_bases
GTGAAACAGTATTTAGATCTCTGTCAGCGTATCGTTAATGACGGTACTTGGATTGAAAATGAACGCACAGGCAAGCGCTG
CCTAACCGTAATCAATGCTGACCTTGAATATGATGTAGGTAATAACCAATTCCCACTGGTAACAACACGTAAAAGTTTTT
GGAAAGCCGCAGTTGCCGAGCTGCTTGGCTATATCCGTGGTTACGACAATGCTGAAGATTTTCGTAAGCTAGGGACTAAA
ACTTGGGATGCGAATTCTAACCTGAATGAAGCATGGCTAAATAACCCTTACCGCAAGGGTGAAGACGACATGGGCCGTGT
TTATGGCGTTCAAGGACGTGCATGGGCAAAACCTGACGGCGGTCATATCGACCAACTGAAGAAGATTGTTGATGATCTAA
CAAACGGTATTGATGACCGCGGCGAGATCTTAAACTTCTATAACCCAGGTGAGTTCCACATGGGTTGTTTGCGTCCGTGT
ATGTACAGCCATCACTTCTCTCTACTTGGTGATACTCTGTACCTAAACAGCACTCAGCGCTCTTGCGATGTACCACTAGG
CCTGAACTTCAACATGGTTCAAGTGTATGTGTTCCTAGCAATCATGGCGCAAATCACGGGTAAGAAAGCGGGTGTGGCTT
ACCACAAGCTGGTTAATGCTCATATCTACGAAGATCAACTCGCACCTATGCGTGATATCCAGTTGAAGCGTGAGCCTTTA
GCGGGGCCAACATTCCATATCAATCCTGAGATTAAGTCTCTAGAAGATTTGGAAACGTGGGTGACGATGGATGACTTCTG
GGTTGAAGGCTACGAATGCCACGAAGCGATTAAGTACCCATTCTCGGTTTAA

Upstream 100 bases:

>100_bases
ACTGCCAATGGTTATCATCGGTGTGCTGATGATGGTTTGGGCATACAAGCGTGGTCACTACAAAGATGAATTACCACAAC
AAACGAAGTAAGGAATTGGT

Downstream 100 bases:

>100_bases
TCTAATCTGCTTTGTTTGTTTGTTTGTTTGTTTGATTGTTTGATTGATTGATTGTTTGTTTGTTTGTTCGACTGATTGAT
CGAAAGGCTGCCTCTGCGCA

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MKQYLDLCQRIVNDGTWIENERTGKRCLTVINADLEYDVGNNQFPLVTTRKSFWKAAVAELLGYIRGYDNAEDFRKLGTK
TWDANSNLNEAWLNNPYRKGEDDMGRVYGVQGRAWAKPDGGHIDQLKKIVDDLTNGIDDRGEILNFYNPGEFHMGCLRPC
MYSHHFSLLGDTLYLNSTQRSCDVPLGLNFNMVQVYVFLAIMAQITGKKAGVAYHKLVNAHIYEDQLAPMRDIQLKREPL
AGPTFHINPEIKSLEDLETWVTMDDFWVEGYECHEAIKYPFSV

Sequences:

>Translated_283_residues
MKQYLDLCQRIVNDGTWIENERTGKRCLTVINADLEYDVGNNQFPLVTTRKSFWKAAVAELLGYIRGYDNAEDFRKLGTK
TWDANSNLNEAWLNNPYRKGEDDMGRVYGVQGRAWAKPDGGHIDQLKKIVDDLTNGIDDRGEILNFYNPGEFHMGCLRPC
MYSHHFSLLGDTLYLNSTQRSCDVPLGLNFNMVQVYVFLAIMAQITGKKAGVAYHKLVNAHIYEDQLAPMRDIQLKREPL
AGPTFHINPEIKSLEDLETWVTMDDFWVEGYECHEAIKYPFSV
>Mature_283_residues
MKQYLDLCQRIVNDGTWIENERTGKRCLTVINADLEYDVGNNQFPLVTTRKSFWKAAVAELLGYIRGYDNAEDFRKLGTK
TWDANSNLNEAWLNNPYRKGEDDMGRVYGVQGRAWAKPDGGHIDQLKKIVDDLTNGIDDRGEILNFYNPGEFHMGCLRPC
MYSHHFSLLGDTLYLNSTQRSCDVPLGLNFNMVQVYVFLAIMAQITGKKAGVAYHKLVNAHIYEDQLAPMRDIQLKREPL
AGPTFHINPEIKSLEDLETWVTMDDFWVEGYECHEAIKYPFSV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily

Homologues:

Organism=Homo sapiens, GI4507751, Length=289, Percent_Identity=33.2179930795848, Blast_Score=156, Evalue=2e-38,
Organism=Escherichia coli, GI1789191, Length=283, Percent_Identity=32.5088339222615, Blast_Score=147, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI71993377, Length=291, Percent_Identity=28.5223367697594, Blast_Score=130, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6324648, Length=303, Percent_Identity=25.4125412541254, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI17137556, Length=291, Percent_Identity=28.5223367697594, Blast_Score=125, Evalue=4e-29,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): TYSY_VIBSL (B7VJ78)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002416182.1
- ProteinModelPortal:   B7VJ78
- SMR:   B7VJ78
- GeneID:   7160159
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_0527
- HOGENOM:   HBG588098
- OMA:   GRAWRKP
- ProtClustDB:   PRK01827
- GO:   GO:0005737
- HAMAP:   MF_00008
- InterPro:   IPR000398
- InterPro:   IPR020940
- Gene3D:   G3DSA:3.30.572.10
- PANTHER:   PTHR11549:SF2
- PRINTS:   PR00108
- TIGRFAMs:   TIGR03284

Pfam domain/function: PF00303 Thymidylat_synt; SSF55831 Thymidylat_synth_C

EC number: =2.1.1.45

Molecular weight: Translated: 32486; Mature: 32486

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: ACT_SITE 160-160

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQYLDLCQRIVNDGTWIENERTGKRCLTVINADLEYDVGNNQFPLVTTRKSFWKAAVAE
CCHHHHHHHHHHCCCCEECCCCCHHHEEEEEECCEEECCCCCCCCEEECHHHHHHHHHHH
LLGYIRGYDNAEDFRKLGTKTWDANSNLNEAWLNNPYRKGEDDMGRVYGVQGRAWAKPDG
HHHHHHCCCCHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCHHHEEECCCCEECCCCC
GHIDQLKKIVDDLTNGIDDRGEILNFYNPGEFHMGCLRPCMYSHHFSLLGDTLYLNSTQR
CCHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCC
SCDVPLGLNFNMVQVYVFLAIMAQITGKKAGVAYHKLVNAHIYEDQLAPMRDIQLKREPL
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCC
AGPTFHINPEIKSLEDLETWVTMDDFWVEGYECHEAIKYPFSV
CCCEEEECCCHHHHHHHHHHHCHHHHHCCCHHHHHHHCCCCCC
>Mature Secondary Structure
MKQYLDLCQRIVNDGTWIENERTGKRCLTVINADLEYDVGNNQFPLVTTRKSFWKAAVAE
CCHHHHHHHHHHCCCCEECCCCCHHHEEEEEECCEEECCCCCCCCEEECHHHHHHHHHHH
LLGYIRGYDNAEDFRKLGTKTWDANSNLNEAWLNNPYRKGEDDMGRVYGVQGRAWAKPDG
HHHHHHCCCCHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCHHHEEECCCCEECCCCC
GHIDQLKKIVDDLTNGIDDRGEILNFYNPGEFHMGCLRPCMYSHHFSLLGDTLYLNSTQR
CCHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCC
SCDVPLGLNFNMVQVYVFLAIMAQITGKKAGVAYHKLVNAHIYEDQLAPMRDIQLKREPL
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCC
AGPTFHINPEIKSLEDLETWVTMDDFWVEGYECHEAIKYPFSV
CCCEEEECCCHHHHHHHHHHHCHHHHHCCCHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA