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The map label for this gene is murE [H]

Identifier: 218708481

GI number: 218708481

Start: 454585

End: 456069

Strand: Direct

Name: murE [H]

Synonym: VS_0445

Alternate gene names: 218708481

Gene position: 454585-456069 (Clockwise)

Preceding gene: 218708480

Following gene: 218708482

Centisome position: 13.78

GC content: 46.73

Gene sequence:

>1485_bases
ATGAGTAATAGCCTCACGCTGTCATCTTTACTTTCTCCTTGGGGGGACTTTAGTTCACTTGAGCTAGATGCGATTGTTGT
TGAGCAATTGGAGTTGGATAGCCGTACCATCAAGGAAGGCGATATTTTTGTTGCCATTGTTGGACATGCCATTGATGGTC
GTCGCTTTATCGACAAAGCCGTCGCTCAAGGTGCGAAAGCTGTCATTGCACAAGCGAGTGATGACAAAGCTCATGGCTTG
GTTGAGTGGTTAAATGCTGTTCCTGTTGTTTATGTATCTGAGCTAAATTCAATTCTCTCTGAACTGGCTGGCCGAGTTTA
TTCTTCTCAAGCGACTAAGTTGATTGGTGTTACCGGCACCAATGGTAAAACCACCATCACCCAATTGATTGCTCAGTGGC
TTGATTTGGTCGGCCAGCGTTCGGCGGTAATGGGCACAACGGGTAATGGCTTCTTAGATAACCTAAAAATAGCGGCTAAT
ACCACGGGCAGCGCGATTGAAATACAACGCACACTGAGCGAGTTGGCTGAGGAAAGTGCAGTTTATACCGCGATGGAAAT
CTCTTCTCATGGTTTGGTTCAAGGCCGAGTAAAAGCGTTGAATTTTGAGGTTGGTGTGTTCACTAACCTGAGTCGTGATC
ACCTTGATTACCACGGCACGATGGAAGAGTACGCACTGGCTAAGAAGAGCCTGTTTACTCAACACAAATGCAAGCACGCA
GTCATTAATGTGGATGACGAAGTAGGTAAAGCTTGGGTGTCAGATTTATCCAATGCCATAGCCGTTTCGCTGTCACCGCT
AACGGGCTATCAACAATCGGTATGGGCGTCTGATGTCGCTTATGCAGAAACCGGTATTCAGCTTTCTTTCGATGGTAGTT
GGGGACAAGGGAAGCTTTCTGTTCCACTGATTGGTCAGTTCAACGCATCAAATGTACTGGTTGCTTTCTCGACTCTGCTT
TCATTGGGCATCGATAAACAAATCTTGGTCGACACTGCATCTCAACTTCAACCTGTGATTGGCCGTATGGAGCTTTTCCA
AACTCCGAACAAAGCAAAAGTGGTTGTCGATTACGCTCATACGCCCGATGCGCTAGAAAAAGCATTGGCAGCATTGCGTG
TGCATTGCTCTGGGCAACTGTGGGCAATCTTCGGCTGTGGTGGTGATCGTGACACCGGTAAGCGCCCAATGATGGCGGTA
ACGGCAGAGCAGTTCGCCGACAAAATTATTATTTCAGATGACAACCCTCGCAGCGAAGATCCTGCTTTGATTGTTAAAGA
CATGCTAGCTGGCTTAAGCGAACCAGAATCTGCATTCGTCGAGCACGATCGCTATCAAGCGGTTAAGTTTGCCCTAGAAC
AGGCAGGCAGTAATGACATTATTCTTTTGGCTGGTAAAGGCCATGAGGATTACCAAGTATTGAAAGACGAAACGATCCAT
TACTCAGATCGAGAGTCCGCGCTACAACTTTTAGGTATTTCATAA

Upstream 100 bases:

>100_bases
TGCCCCCGTTTTTTCTGAAATCATGAAGGGGGCACTGCAAATACTCAATGTCCCTGCTGATGAAAACAAGTTTCAAGAAT
AGAGCCAATCAGGATTCAAT

Downstream 100 bases:

>100_bases
TGATTGATGTATCACTCGAGCAGATTTGTTCAGCGGTGAGCGGTCAGCTGATTGAGGCTGGCAAGTCGAGCAATAGTGTT
ATTAATGCGGTTTCTACTGA

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 494; Mature: 493

Protein sequence:

>494_residues
MSNSLTLSSLLSPWGDFSSLELDAIVVEQLELDSRTIKEGDIFVAIVGHAIDGRRFIDKAVAQGAKAVIAQASDDKAHGL
VEWLNAVPVVYVSELNSILSELAGRVYSSQATKLIGVTGTNGKTTITQLIAQWLDLVGQRSAVMGTTGNGFLDNLKIAAN
TTGSAIEIQRTLSELAEESAVYTAMEISSHGLVQGRVKALNFEVGVFTNLSRDHLDYHGTMEEYALAKKSLFTQHKCKHA
VINVDDEVGKAWVSDLSNAIAVSLSPLTGYQQSVWASDVAYAETGIQLSFDGSWGQGKLSVPLIGQFNASNVLVAFSTLL
SLGIDKQILVDTASQLQPVIGRMELFQTPNKAKVVVDYAHTPDALEKALAALRVHCSGQLWAIFGCGGDRDTGKRPMMAV
TAEQFADKIIISDDNPRSEDPALIVKDMLAGLSEPESAFVEHDRYQAVKFALEQAGSNDIILLAGKGHEDYQVLKDETIH
YSDRESALQLLGIS

Sequences:

>Translated_494_residues
MSNSLTLSSLLSPWGDFSSLELDAIVVEQLELDSRTIKEGDIFVAIVGHAIDGRRFIDKAVAQGAKAVIAQASDDKAHGL
VEWLNAVPVVYVSELNSILSELAGRVYSSQATKLIGVTGTNGKTTITQLIAQWLDLVGQRSAVMGTTGNGFLDNLKIAAN
TTGSAIEIQRTLSELAEESAVYTAMEISSHGLVQGRVKALNFEVGVFTNLSRDHLDYHGTMEEYALAKKSLFTQHKCKHA
VINVDDEVGKAWVSDLSNAIAVSLSPLTGYQQSVWASDVAYAETGIQLSFDGSWGQGKLSVPLIGQFNASNVLVAFSTLL
SLGIDKQILVDTASQLQPVIGRMELFQTPNKAKVVVDYAHTPDALEKALAALRVHCSGQLWAIFGCGGDRDTGKRPMMAV
TAEQFADKIIISDDNPRSEDPALIVKDMLAGLSEPESAFVEHDRYQAVKFALEQAGSNDIILLAGKGHEDYQVLKDETIH
YSDRESALQLLGIS
>Mature_493_residues
SNSLTLSSLLSPWGDFSSLELDAIVVEQLELDSRTIKEGDIFVAIVGHAIDGRRFIDKAVAQGAKAVIAQASDDKAHGLV
EWLNAVPVVYVSELNSILSELAGRVYSSQATKLIGVTGTNGKTTITQLIAQWLDLVGQRSAVMGTTGNGFLDNLKIAANT
TGSAIEIQRTLSELAEESAVYTAMEISSHGLVQGRVKALNFEVGVFTNLSRDHLDYHGTMEEYALAKKSLFTQHKCKHAV
INVDDEVGKAWVSDLSNAIAVSLSPLTGYQQSVWASDVAYAETGIQLSFDGSWGQGKLSVPLIGQFNASNVLVAFSTLLS
LGIDKQILVDTASQLQPVIGRMELFQTPNKAKVVVDYAHTPDALEKALAALRVHCSGQLWAIFGCGGDRDTGKRPMMAVT
AEQFADKIIISDDNPRSEDPALIVKDMLAGLSEPESAFVEHDRYQAVKFALEQAGSNDIILLAGKGHEDYQVLKDETIHY
SDRESALQLLGIS

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=494, Percent_Identity=52.0242914979757, Blast_Score=483, Evalue=1e-137,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 53266; Mature: 53135

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNSLTLSSLLSPWGDFSSLELDAIVVEQLELDSRTIKEGDIFVAIVGHAIDGRRFIDKA
CCCCEEHHHHHCCCCCCCCCEEHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHH
VAQGAKAVIAQASDDKAHGLVEWLNAVPVVYVSELNSILSELAGRVYSSQATKLIGVTGT
HHCCCCEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECC
NGKTTITQLIAQWLDLVGQRSAVMGTTGNGFLDNLKIAANTTGSAIEIQRTLSELAEESA
CCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHH
VYTAMEISSHGLVQGRVKALNFEVGVFTNLSRDHLDYHGTMEEYALAKKSLFTQHKCKHA
HEEEEECCCCCCEECEEEEEEEEEEEEECCCHHHCCCCCCHHHHHHHHHHHHHHHCCCEE
VINVDDEVGKAWVSDLSNAIAVSLSPLTGYQQSVWASDVAYAETGIQLSFDGSWGQGKLS
EEECCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCCEEE
VPLIGQFNASNVLVAFSTLLSLGIDKQILVDTASQLQPVIGRMELFQTPNKAKVVVDYAH
EEEEECCCCCCHHHHHHHHHHHCCCCHHHHHCHHHHHHHHHHHHHHCCCCCCEEEEEECC
TPDALEKALAALRVHCSGQLWAIFGCGGDRDTGKRPMMAVTAEQFADKIIISDDNPRSED
CCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCEEEEEHHHHHCEEEEECCCCCCCC
PALIVKDMLAGLSEPESAFVEHDRYQAVKFALEQAGSNDIILLAGKGHEDYQVLKDETIH
CHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHCCCCCC
YSDRESALQLLGIS
CCCHHHHHHHHCCC
>Mature Secondary Structure 
SNSLTLSSLLSPWGDFSSLELDAIVVEQLELDSRTIKEGDIFVAIVGHAIDGRRFIDKA
CCCEEHHHHHCCCCCCCCCEEHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHH
VAQGAKAVIAQASDDKAHGLVEWLNAVPVVYVSELNSILSELAGRVYSSQATKLIGVTGT
HHCCCCEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECC
NGKTTITQLIAQWLDLVGQRSAVMGTTGNGFLDNLKIAANTTGSAIEIQRTLSELAEESA
CCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHH
VYTAMEISSHGLVQGRVKALNFEVGVFTNLSRDHLDYHGTMEEYALAKKSLFTQHKCKHA
HEEEEECCCCCCEECEEEEEEEEEEEEECCCHHHCCCCCCHHHHHHHHHHHHHHHCCCEE
VINVDDEVGKAWVSDLSNAIAVSLSPLTGYQQSVWASDVAYAETGIQLSFDGSWGQGKLS
EEECCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCCEEE
VPLIGQFNASNVLVAFSTLLSLGIDKQILVDTASQLQPVIGRMELFQTPNKAKVVVDYAH
EEEEECCCCCCHHHHHHHHHHHCCCCHHHHHCHHHHHHHHHHHHHHCCCCCCEEEEEECC
TPDALEKALAALRVHCSGQLWAIFGCGGDRDTGKRPMMAVTAEQFADKIIISDDNPRSED
CCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCEEEEEHHHHHCEEEEECCCCCCCC
PALIVKDMLAGLSEPESAFVEHDRYQAVKFALEQAGSNDIILLAGKGHEDYQVLKDETIH
CHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHCCCCCC
YSDRESALQLLGIS
CCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]