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The map label for this gene is ilvI [H]

Identifier: 218708426

GI number: 218708426

Start: 392575

End: 394332

Strand: Direct

Name: ilvI [H]

Synonym: VS_0388

Alternate gene names: 218708426

Gene position: 392575-394332 (Clockwise)

Preceding gene: 218708425

Following gene: 218708427

Centisome position: 11.9

GC content: 44.14

Gene sequence:

>1758_bases
ATGACAACAAAACCTGAAACAGCCATGTCATCCGGCGCTGAAATGTTATCCGGCGCTGAGATGGTGGTGCAGTCTCTAAT
TGAAGAGGGTGTAGAACAAATCTTTGGTTATCCAGGTGGTTCTGTACTTGATATCTACGATGCGCTGCATGCTAAAACTG
CTGAAATTAAACACGTATTAGTACGACACGAACAAGCCGCTACCCACATGGCAGATGGCTATACTCGTTCTACCGGTAAG
CCGGGTGTAGTACTTGTGTGTTCTGGTCCAGGTGCGACCAATACCGTTACTGGTATTGCAACAGCCTACATGGACTCAAT
CCCAATGATTGTTATTTCTGGTAACGTACCAAATAACCTTATTGGTAACGACGCCTTCCAAGAGTGTGACATCGTTGGTG
TATCACGCCCGATCGTTAAACACAGCTTCCTCGTTAAGAAAGCGGAAGATATCCCTGAAGTCGTTAAAAAAGCATTCTAT
ATTTCAACGACAGGACGTCCAGGTCCTGTGGTTATTGATCTGCCAAAAGACATTTTAAACCCACAAATTAAGCTTCCTTA
TGAATACCCAGAAACGATCAAAATGCGTTCATATAACCCAACGGTTACGGGTCATAAAGGTCAGATCAAAAAGGCGCTAA
AAGCTCTTCTTGAAGCGAAGAAGCCGGTACTTTATGTCGGTGGTGGTGCGGTTATTTCTGAGGCAGATAAGCCGCTGTTA
AAATTAGCAGAGGCACTGAATTTACCCGTGGTAAGTACCCTAATGGGGCTTGGCGCTTTCCCTGGTACTCATGAGAACTC
TTTGGGTATGTTGGGCATGCATGGTTTGTATGAAGCCAATATGGCGATGCACAATGCGGACTTGATCTTTGGTATTGGCG
TACGTTTCGATGATCGAACGACCAATAACCTAGATAAATACTGTCCGGATGCGAAGATCATGCACATTGATATCGATCCA
TCTTCTATCTCTAAGAACGTTAAAGTGGATCTACCGATTGTAGGTTCAGCGGAAAAAGTACTAGAAAGCATGGTTAACCT
GCTTATTGAGCAAGGTGGAACTAACGATGCAGAAGCGATGGAAAGCTGGTGGAGTGAAATTAAACAGTGGCAAGAACGTG
ACTGCTTAGCGTATGACAAAACGTCGGGTCGAATTAAGCCACAACAAGTTATTGAAACGCTGCATAAAGTAACCAATGGC
GATGCCTATGTTGCTTCTGATGTAGGTCAACACCAAATGTTTGCTGCTTTGTATTACCCGTTTGACAAGCCGCGCCGTTG
GATTAACTCTGGTGGTCTAGGTACGATGGGCTTTGGCTTACCAGCTGGTATGGGCGTTAAGTTTGCGAAACCTGATGAAG
AAGTTGTGATTGTTACTGGTGACGGCAGTATTCAGATGAATATCCAAGAGCTGTCGACTGCGCTGCAATACAATATCCCC
GTTAAGATTATTAATCTTAACAACCGTTTCTTAGGCATGGTAAAACAGTGGCAAGACATTGTTTATCAAGGTCGTTACTC
TAATTCATATATGGACTCTGTTCCTGATTTTGCTGCTATCGCTGAGGCTTATGGCCACGTTGGCATGCGTATTTCATCTC
CGGATGAATTGGAATCAGGTTTGGAAAAAGCACTAGCGATGAAAGACAGTTTGGTATTTGTCGATATTAGTGTGGATGAC
ACCGAGCACGTATACCCAATGCAGATCAAAGGCGAGGGTATGGATAACATGTGGCTAAGCAAAACGGAGAGAACATAA

Upstream 100 bases:

>100_bases
GTGGAAGTATTTCGAATTAGGCTACGAATAAGCCCTAGACTATGAAAAACCAGCCCAACATGTTCACCAAATGTGATTGG
AAACTGGTGAGGAGAGCAAT

Downstream 100 bases:

>100_bases
TATGAGACACATCCTTTCACTATTAATGGAAAACCAACCTGGTGCACTATCTCGTGTGGTTGGCTTGTTTTCTCAGCGTG
GCTACAACATCGAGTCTTTG

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS-III; ALS-III; Acetohydroxy-acid synthase III large subunit [H]

Number of amino acids: Translated: 585; Mature: 584

Protein sequence:

>585_residues
MTTKPETAMSSGAEMLSGAEMVVQSLIEEGVEQIFGYPGGSVLDIYDALHAKTAEIKHVLVRHEQAATHMADGYTRSTGK
PGVVLVCSGPGATNTVTGIATAYMDSIPMIVISGNVPNNLIGNDAFQECDIVGVSRPIVKHSFLVKKAEDIPEVVKKAFY
ISTTGRPGPVVIDLPKDILNPQIKLPYEYPETIKMRSYNPTVTGHKGQIKKALKALLEAKKPVLYVGGGAVISEADKPLL
KLAEALNLPVVSTLMGLGAFPGTHENSLGMLGMHGLYEANMAMHNADLIFGIGVRFDDRTTNNLDKYCPDAKIMHIDIDP
SSISKNVKVDLPIVGSAEKVLESMVNLLIEQGGTNDAEAMESWWSEIKQWQERDCLAYDKTSGRIKPQQVIETLHKVTNG
DAYVASDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAGMGVKFAKPDEEVVIVTGDGSIQMNIQELSTALQYNIP
VKIINLNNRFLGMVKQWQDIVYQGRYSNSYMDSVPDFAAIAEAYGHVGMRISSPDELESGLEKALAMKDSLVFVDISVDD
TEHVYPMQIKGEGMDNMWLSKTERT

Sequences:

>Translated_585_residues
MTTKPETAMSSGAEMLSGAEMVVQSLIEEGVEQIFGYPGGSVLDIYDALHAKTAEIKHVLVRHEQAATHMADGYTRSTGK
PGVVLVCSGPGATNTVTGIATAYMDSIPMIVISGNVPNNLIGNDAFQECDIVGVSRPIVKHSFLVKKAEDIPEVVKKAFY
ISTTGRPGPVVIDLPKDILNPQIKLPYEYPETIKMRSYNPTVTGHKGQIKKALKALLEAKKPVLYVGGGAVISEADKPLL
KLAEALNLPVVSTLMGLGAFPGTHENSLGMLGMHGLYEANMAMHNADLIFGIGVRFDDRTTNNLDKYCPDAKIMHIDIDP
SSISKNVKVDLPIVGSAEKVLESMVNLLIEQGGTNDAEAMESWWSEIKQWQERDCLAYDKTSGRIKPQQVIETLHKVTNG
DAYVASDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAGMGVKFAKPDEEVVIVTGDGSIQMNIQELSTALQYNIP
VKIINLNNRFLGMVKQWQDIVYQGRYSNSYMDSVPDFAAIAEAYGHVGMRISSPDELESGLEKALAMKDSLVFVDISVDD
TEHVYPMQIKGEGMDNMWLSKTERT
>Mature_584_residues
TTKPETAMSSGAEMLSGAEMVVQSLIEEGVEQIFGYPGGSVLDIYDALHAKTAEIKHVLVRHEQAATHMADGYTRSTGKP
GVVLVCSGPGATNTVTGIATAYMDSIPMIVISGNVPNNLIGNDAFQECDIVGVSRPIVKHSFLVKKAEDIPEVVKKAFYI
STTGRPGPVVIDLPKDILNPQIKLPYEYPETIKMRSYNPTVTGHKGQIKKALKALLEAKKPVLYVGGGAVISEADKPLLK
LAEALNLPVVSTLMGLGAFPGTHENSLGMLGMHGLYEANMAMHNADLIFGIGVRFDDRTTNNLDKYCPDAKIMHIDIDPS
SISKNVKVDLPIVGSAEKVLESMVNLLIEQGGTNDAEAMESWWSEIKQWQERDCLAYDKTSGRIKPQQVIETLHKVTNGD
AYVASDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAGMGVKFAKPDEEVVIVTGDGSIQMNIQELSTALQYNIPV
KIINLNNRFLGMVKQWQDIVYQGRYSNSYMDSVPDFAAIAEAYGHVGMRISSPDELESGLEKALAMKDSLVFVDISVDDT
EHVYPMQIKGEGMDNMWLSKTERT

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=597, Percent_Identity=27.3031825795645, Blast_Score=203, Evalue=3e-52,
Organism=Homo sapiens, GI21361361, Length=512, Percent_Identity=24.609375, Blast_Score=144, Evalue=3e-34,
Organism=Escherichia coli, GI87081685, Length=574, Percent_Identity=70.383275261324, Blast_Score=874, Evalue=0.0,
Organism=Escherichia coli, GI1790104, Length=562, Percent_Identity=39.3238434163701, Blast_Score=421, Evalue=1e-119,
Organism=Escherichia coli, GI1786717, Length=533, Percent_Identity=33.9587242026266, Blast_Score=322, Evalue=4e-89,
Organism=Escherichia coli, GI1787096, Length=559, Percent_Identity=28.8014311270125, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI1788716, Length=562, Percent_Identity=25.2669039145907, Blast_Score=185, Evalue=8e-48,
Organism=Caenorhabditis elegans, GI17531301, Length=585, Percent_Identity=25.8119658119658, Blast_Score=176, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17531299, Length=585, Percent_Identity=25.8119658119658, Blast_Score=176, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17542570, Length=589, Percent_Identity=27.6740237691002, Blast_Score=159, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6323755, Length=595, Percent_Identity=41.344537815126, Blast_Score=447, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6320816, Length=547, Percent_Identity=24.1316270566728, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6320588, Length=313, Percent_Identity=23.6421725239617, Blast_Score=64, Evalue=9e-11,
Organism=Drosophila melanogaster, GI19922626, Length=594, Percent_Identity=25.9259259259259, Blast_Score=185, Evalue=7e-47,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 63865; Mature: 63734

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTKPETAMSSGAEMLSGAEMVVQSLIEEGVEQIFGYPGGSVLDIYDALHAKTAEIKHVL
CCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
VRHEQAATHMADGYTRSTGKPGVVLVCSGPGATNTVTGIATAYMDSIPMIVISGNVPNNL
HHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCC
IGNDAFQECDIVGVSRPIVKHSFLVKKAEDIPEVVKKAFYISTTGRPGPVVIDLPKDILN
CCCCHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEECCHHHCC
PQIKLPYEYPETIKMRSYNPTVTGHKGQIKKALKALLEAKKPVLYVGGGAVISEADKPLL
CCEECCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCEECCCCHHHH
KLAEALNLPVVSTLMGLGAFPGTHENSLGMLGMHGLYEANMAMHNADLIFGIGVRFDDRT
HHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHEECCCEEEEEEEEECCCC
TNNLDKYCPDAKIMHIDIDPSSISKNVKVDLPIVGSAEKVLESMVNLLIEQGGTNDAEAM
CCCHHHHCCCCEEEEEECCHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCHHHHH
ESWWSEIKQWQERDCLAYDKTSGRIKPQQVIETLHKVTNGDAYVASDVGQHQMFAALYYP
HHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCHHHEEHHEECC
FDKPRRWINSGGLGTMGFGLPAGMGVKFAKPDEEVVIVTGDGSIQMNIQELSTALQYNIP
CHHHHHHHCCCCCCCCCCCCCCCCCCEECCCCCCEEEEECCCCEEEEHHHHHHHHEECCC
VKIINLNNRFLGMVKQWQDIVYQGRYSNSYMDSVPDFAAIAEAYGHVGMRISSPDELESG
EEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHCCCHHHHHHHHHCCCCEEECCHHHHHHH
LEKALAMKDSLVFVDISVDDTEHVYPMQIKGEGMDNMWLSKTERT
HHHHHHHCCCEEEEEEEECCCCCEEEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
TTKPETAMSSGAEMLSGAEMVVQSLIEEGVEQIFGYPGGSVLDIYDALHAKTAEIKHVL
CCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
VRHEQAATHMADGYTRSTGKPGVVLVCSGPGATNTVTGIATAYMDSIPMIVISGNVPNNL
HHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCC
IGNDAFQECDIVGVSRPIVKHSFLVKKAEDIPEVVKKAFYISTTGRPGPVVIDLPKDILN
CCCCHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEECCHHHCC
PQIKLPYEYPETIKMRSYNPTVTGHKGQIKKALKALLEAKKPVLYVGGGAVISEADKPLL
CCEECCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCEECCCCHHHH
KLAEALNLPVVSTLMGLGAFPGTHENSLGMLGMHGLYEANMAMHNADLIFGIGVRFDDRT
HHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHEECCCEEEEEEEEECCCC
TNNLDKYCPDAKIMHIDIDPSSISKNVKVDLPIVGSAEKVLESMVNLLIEQGGTNDAEAM
CCCHHHHCCCCEEEEEECCHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCHHHHH
ESWWSEIKQWQERDCLAYDKTSGRIKPQQVIETLHKVTNGDAYVASDVGQHQMFAALYYP
HHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCHHHEEHHEECC
FDKPRRWINSGGLGTMGFGLPAGMGVKFAKPDEEVVIVTGDGSIQMNIQELSTALQYNIP
CHHHHHHHCCCCCCCCCCCCCCCCCCEECCCCCCEEEEECCCCEEEEHHHHHHHHEECCC
VKIINLNNRFLGMVKQWQDIVYQGRYSNSYMDSVPDFAAIAEAYGHVGMRISSPDELESG
EEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHCCCHHHHHHHHHCCCCEEECCHHHHHHH
LEKALAMKDSLVFVDISVDDTEHVYPMQIKGEGMDNMWLSKTERT
HHHHHHHCCCEEEEEEEECCCCCEEEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308579; 1630901; 9278503; 3891724; 9298646 [H]