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The map label for this gene is mdh [H]

Identifier: 218708398

GI number: 218708398

Start: 359993

End: 360928

Strand: Direct

Name: mdh [H]

Synonym: VS_0358

Alternate gene names: 218708398

Gene position: 359993-360928 (Clockwise)

Preceding gene: 218708396

Following gene: 218708399

Centisome position: 10.91

GC content: 47.97

Gene sequence:

>936_bases
ATGAAAGTAGCTGTTATTGGTGCCGCTGGTGGCATCGGTCAAGCCCTAGCCCTACTACTAAAGAACCGCCTGCCTGCTGG
TTCAGATCTTGCACTTTACGACATCGCTCCGGTAACCCCGGGGGTTGCTGCCGATCTTAGCCATATCCCAACGCCTGTTT
CGATCAAAGGTTACGCGGGTGAAGATCCAACTCCAGCACTTGAAGGTGCGGATGTTGTGCTTATTTCTGCGGGTGTTGCT
CGTAAACCTGGTATGGATCGTGCGGATCTTTTCAATGTGAACGCTGGCATTGTTAAGTCTCTAGCTGAAAAAATTGCCGT
TACTTGTCCTACTGCTTGTGTTGGTATCATTACTAACCCAGTAAATACAACAGTGCCAATCGCTGCTGAAGTACTTAAGA
AAGCGGGCGTTTACGACAAGCGTCGTTTATTCGGTATTACTACTCTTGATGTCATTCGTTCTGAAACGTTCGTTGCTGAG
TTGAAAGATAAAGATCCAGGCGACATTCGTGTTCCTGTTATCGGTGGTCACTCAGGTGTAACGATCCTTCCTCTTCTTTC
TCAAGTTGAAGGCGTAGAGTTCACTGATGAAGAAATCGCGGCGCTAACAACTCGCATCCAAAACGCGGGTACTGAAGTAG
TAGAAGCTAAAGCGGGTGGCGGTAGTGCGACTCTATCTATGGGTCAAGCGGCTTGTCGCTTCGGTCTAGCTCTAGTGAAA
GCACTTCAAGGCGAAGAAAACGTGATTGAGTGTGCCTACGTTGAAGGTGAAGGCGAGCACGCACCATTCTTCGCACAACC
AGTTAAACTTGGCAAAGAAGGCGCTGAAGCGATTCTTAGCTACGGTGAACTGAGCGACTTCGAACGCAATGCACTAGACA
GCATGCTAGAAACGCTAAATGGCGATATTGAGATTGGTGTTGAATTCGCTAAATAA

Upstream 100 bases:

>100_bases
TTGTAATCACTTTGTGATTAATATAGTTTTTTAACTTAAATTTAGCTCAAGAATTACCCTACAAAGAATCTATAAGAGAA
AGCTCTCAAGGAGAATAACA

Downstream 100 bases:

>100_bases
GCGATAGATAACTGAACTCTGCTAAAACGACAGTTAACGAAAGCCGATCATTGCGATCGGCTTTTTTGATCCTTGTATTC
AACCCACTCGTAATCTTTTT

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MKVAVIGAAGGIGQALALLLKNRLPAGSDLALYDIAPVTPGVAADLSHIPTPVSIKGYAGEDPTPALEGADVVLISAGVA
RKPGMDRADLFNVNAGIVKSLAEKIAVTCPTACVGIITNPVNTTVPIAAEVLKKAGVYDKRRLFGITTLDVIRSETFVAE
LKDKDPGDIRVPVIGGHSGVTILPLLSQVEGVEFTDEEIAALTTRIQNAGTEVVEAKAGGGSATLSMGQAACRFGLALVK
ALQGEENVIECAYVEGEGEHAPFFAQPVKLGKEGAEAILSYGELSDFERNALDSMLETLNGDIEIGVEFAK

Sequences:

>Translated_311_residues
MKVAVIGAAGGIGQALALLLKNRLPAGSDLALYDIAPVTPGVAADLSHIPTPVSIKGYAGEDPTPALEGADVVLISAGVA
RKPGMDRADLFNVNAGIVKSLAEKIAVTCPTACVGIITNPVNTTVPIAAEVLKKAGVYDKRRLFGITTLDVIRSETFVAE
LKDKDPGDIRVPVIGGHSGVTILPLLSQVEGVEFTDEEIAALTTRIQNAGTEVVEAKAGGGSATLSMGQAACRFGLALVK
ALQGEENVIECAYVEGEGEHAPFFAQPVKLGKEGAEAILSYGELSDFERNALDSMLETLNGDIEIGVEFAK
>Mature_311_residues
MKVAVIGAAGGIGQALALLLKNRLPAGSDLALYDIAPVTPGVAADLSHIPTPVSIKGYAGEDPTPALEGADVVLISAGVA
RKPGMDRADLFNVNAGIVKSLAEKIAVTCPTACVGIITNPVNTTVPIAAEVLKKAGVYDKRRLFGITTLDVIRSETFVAE
LKDKDPGDIRVPVIGGHSGVTILPLLSQVEGVEFTDEEIAALTTRIQNAGTEVVEAKAGGGSATLSMGQAACRFGLALVK
ALQGEENVIECAYVEGEGEHAPFFAQPVKLGKEGAEAILSYGELSDFERNALDSMLETLNGDIEIGVEFAK

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 1 family [H]

Homologues:

Organism=Homo sapiens, GI21735621, Length=312, Percent_Identity=54.4871794871795, Blast_Score=323, Evalue=2e-88,
Organism=Escherichia coli, GI1789632, Length=309, Percent_Identity=77.6699029126214, Blast_Score=489, Evalue=1e-139,
Organism=Caenorhabditis elegans, GI17554310, Length=313, Percent_Identity=56.5495207667732, Blast_Score=326, Evalue=8e-90,
Organism=Saccharomyces cerevisiae, GI6322765, Length=314, Percent_Identity=50.3184713375796, Blast_Score=272, Evalue=6e-74,
Organism=Saccharomyces cerevisiae, GI6324446, Length=364, Percent_Identity=37.9120879120879, Blast_Score=192, Evalue=5e-50,
Organism=Saccharomyces cerevisiae, GI6320125, Length=337, Percent_Identity=38.5756676557863, Blast_Score=187, Evalue=1e-48,
Organism=Drosophila melanogaster, GI24647881, Length=311, Percent_Identity=56.5916398713826, Blast_Score=330, Evalue=7e-91,
Organism=Drosophila melanogaster, GI24663599, Length=313, Percent_Identity=49.8402555910543, Blast_Score=277, Evalue=7e-75,
Organism=Drosophila melanogaster, GI24663595, Length=300, Percent_Identity=47, Blast_Score=262, Evalue=2e-70,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR001252
- InterPro:   IPR010097
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 32203; Mature: 32203

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: PS00068 MDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVAVIGAAGGIGQALALLLKNRLPAGSDLALYDIAPVTPGVAADLSHIPTPVSIKGYAG
CEEEEEECCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHCCCCEEEECCCC
EDPTPALEGADVVLISAGVARKPGMDRADLFNVNAGIVKSLAEKIAVTCPTACVGIITNP
CCCCCCCCCCCEEEEECCCCCCCCCCHHHEEECCHHHHHHHHHHHEECCCHHHHHEEECC
VNTTVPIAAEVLKKAGVYDKRRLFGITTLDVIRSETFVAELKDKDPGDIRVPVIGGHSGV
CCCCHHHHHHHHHHCCCCCHHHEECCHHHHHHHHHHHEEECCCCCCCCEEEEEEECCCCC
TILPLLSQVEGVEFTDEEIAALTTRIQNAGTEVVEAKAGGGSATLSMGQAACRFGLALVK
CHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHH
ALQGEENVIECAYVEGEGEHAPFFAQPVKLGKEGAEAILSYGELSDFERNALDSMLETLN
HHCCCCCEEEEEEEECCCCCCCEEHHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHHHHHC
GDIEIGVEFAK
CCEEEEEEECC
>Mature Secondary Structure
MKVAVIGAAGGIGQALALLLKNRLPAGSDLALYDIAPVTPGVAADLSHIPTPVSIKGYAG
CEEEEEECCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHCCCCEEEECCCC
EDPTPALEGADVVLISAGVARKPGMDRADLFNVNAGIVKSLAEKIAVTCPTACVGIITNP
CCCCCCCCCCCEEEEECCCCCCCCCCHHHEEECCHHHHHHHHHHHEECCCHHHHHEEECC
VNTTVPIAAEVLKKAGVYDKRRLFGITTLDVIRSETFVAELKDKDPGDIRVPVIGGHSGV
CCCCHHHHHHHHHHCCCCCHHHEECCHHHHHHHHHHHEEECCCCCCCCEEEEEEECCCCC
TILPLLSQVEGVEFTDEEIAALTTRIQNAGTEVVEAKAGGGSATLSMGQAACRFGLALVK
CHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHH
ALQGEENVIECAYVEGEGEHAPFFAQPVKLGKEGAEAILSYGELSDFERNALDSMLETLN
HHCCCCCEEEEEEEECCCCCCCEEHHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHHHHHC
GDIEIGVEFAK
CCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA