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The map label for this gene is yiaE [C]

Identifier: 218708392

GI number: 218708392

Start: 353442

End: 354404

Strand: Direct

Name: yiaE [C]

Synonym: VS_0352

Alternate gene names: 218708392

Gene position: 353442-354404 (Clockwise)

Preceding gene: 218708391

Following gene: 218708396

Centisome position: 10.71

GC content: 45.38

Gene sequence:

>963_bases
ATGCCGAAGTTGAAAGTGGTCTTTCTCGACAGAGCTACCATCCCATCTCAAATACATTTAAAACCCCTTAGCTTTGAGCA
TCAGTGGGTGGAGTATGACTTCACTTCGCCAGAACAAGTGTCTGAACGAATTGAAGGCGCTGATGTGGTTATTACCAACA
AGGTCGTGCTTAACGAATCGAATTTGGCTCAAGCACAACAACTTAAGCTAATAGCTGTTTCAGCTACGGGTGTGAACAAT
GTCGATGTTGGCTACTGCAAGAGTAACAACATAGCGGTGACAAACGTGCAAGGCTACGCGACTCAATCGGTACCTGAACA
TGTTATCGCGATGCTGTTTACGTTAAAGCGAAACCTCGTTGGTTATCATCAGGACATCAAGGCGGGCGAATGGCAAAAGG
ACAAACAGTTCTGCTTTTTCACTCATCCGATCCAAGATGTTGCAGGCAGCACATTGGGCTTAATCGGGAGTGGCAGTTTA
GGGCAAGCGACCGCGATATTAGCAAAATCGATTGGTATGAACGTCATCTTTGCTGAGCGTAAAGAGGCAGGTTCTTGTCG
AGAAGGGTATCTACCTTTTGAGGCTGTATTACAGCAAGCAGATGCGATCAGCTTACACTGCCCGTTGACTGAAGCGACTC
GAGATCTGATATCGGAGCGAGAGCTTACCATGATGAAATCAAGTGCGGTATTAATTAATGCTGGCCGTGGTGGACTTGTC
GATGAGCAGGCCTTAGTTGAAGCTTTGAAAAGTAATGAGATTGCAGGTGCGGGCATGGATGTGTTTACACAAGAACCTGC
AGATAATTCGAATCCACTATTGGCTAACAGCCACTTACCTAATTTACTGCTAACACCACATGTGGCGTGGGGCAGCGACA
GTTCAATTCAAAAACTGTCTGATATCTTAATCGATAATATTGATGGGTTTGTTGCAGGTAATCCGCAGAACTTAGTGATT
TGA

Upstream 100 bases:

>100_bases
CCACAAATCGAGCAGCACTTTGAAATTGCGCCAGAGCTTCCAGACTACAGTAAGCTAAAAGCTTAGAATGGTGTCATCAA
TACCAATGCAAGGACGTACA

Downstream 100 bases:

>100_bases
TTAGAAGTATTTAATGGTGCAAGCACAAAATAAAAAAAGGTTGGCTTTTAGGCCAACCTTTTTAGATTTAGATCGTTGTG
TCGATTAAGTGATTTAGCCT

Product: putative 2-hydroxyacid dehydrogenase family protein

Products: glycerate; NADP; NADPH; glyoxylate; 2-Dehydro-D-gluconate; 5-ketogluconate; L-idonate [C]

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MPKLKVVFLDRATIPSQIHLKPLSFEHQWVEYDFTSPEQVSERIEGADVVITNKVVLNESNLAQAQQLKLIAVSATGVNN
VDVGYCKSNNIAVTNVQGYATQSVPEHVIAMLFTLKRNLVGYHQDIKAGEWQKDKQFCFFTHPIQDVAGSTLGLIGSGSL
GQATAILAKSIGMNVIFAERKEAGSCREGYLPFEAVLQQADAISLHCPLTEATRDLISERELTMMKSSAVLINAGRGGLV
DEQALVEALKSNEIAGAGMDVFTQEPADNSNPLLANSHLPNLLLTPHVAWGSDSSIQKLSDILIDNIDGFVAGNPQNLVI

Sequences:

>Translated_320_residues
MPKLKVVFLDRATIPSQIHLKPLSFEHQWVEYDFTSPEQVSERIEGADVVITNKVVLNESNLAQAQQLKLIAVSATGVNN
VDVGYCKSNNIAVTNVQGYATQSVPEHVIAMLFTLKRNLVGYHQDIKAGEWQKDKQFCFFTHPIQDVAGSTLGLIGSGSL
GQATAILAKSIGMNVIFAERKEAGSCREGYLPFEAVLQQADAISLHCPLTEATRDLISERELTMMKSSAVLINAGRGGLV
DEQALVEALKSNEIAGAGMDVFTQEPADNSNPLLANSHLPNLLLTPHVAWGSDSSIQKLSDILIDNIDGFVAGNPQNLVI
>Mature_319_residues
PKLKVVFLDRATIPSQIHLKPLSFEHQWVEYDFTSPEQVSERIEGADVVITNKVVLNESNLAQAQQLKLIAVSATGVNNV
DVGYCKSNNIAVTNVQGYATQSVPEHVIAMLFTLKRNLVGYHQDIKAGEWQKDKQFCFFTHPIQDVAGSTLGLIGSGSLG
QATAILAKSIGMNVIFAERKEAGSCREGYLPFEAVLQQADAISLHCPLTEATRDLISERELTMMKSSAVLINAGRGGLVD
EQALVEALKSNEIAGAGMDVFTQEPADNSNPLLANSHLPNLLLTPHVAWGSDSSIQKLSDILIDNIDGFVAGNPQNLVI

Specific function: Unknown

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI145580578, Length=297, Percent_Identity=31.3131313131313, Blast_Score=141, Evalue=8e-34,
Organism=Homo sapiens, GI4557499, Length=297, Percent_Identity=31.3131313131313, Blast_Score=141, Evalue=8e-34,
Organism=Homo sapiens, GI145580575, Length=297, Percent_Identity=31.3131313131313, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI61743967, Length=273, Percent_Identity=31.5018315018315, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI4557497, Length=273, Percent_Identity=31.5018315018315, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI23308577, Length=240, Percent_Identity=28.3333333333333, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI6912396, Length=258, Percent_Identity=28.6821705426357, Blast_Score=104, Evalue=1e-22,
Organism=Escherichia coli, GI87082289, Length=270, Percent_Identity=31.1111111111111, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1787645, Length=317, Percent_Identity=29.6529968454259, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1789279, Length=272, Percent_Identity=33.8235294117647, Blast_Score=125, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17532191, Length=255, Percent_Identity=31.3725490196078, Blast_Score=120, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI25147481, Length=305, Percent_Identity=27.5409836065574, Blast_Score=109, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6320925, Length=288, Percent_Identity=31.5972222222222, Blast_Score=141, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6322116, Length=286, Percent_Identity=31.8181818181818, Blast_Score=139, Evalue=7e-34,
Organism=Saccharomyces cerevisiae, GI6324055, Length=256, Percent_Identity=31.25, Blast_Score=107, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6324964, Length=295, Percent_Identity=26.1016949152542, Blast_Score=100, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6325144, Length=179, Percent_Identity=28.4916201117318, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324980, Length=80, Percent_Identity=33.75, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24646446, Length=292, Percent_Identity=31.5068493150685, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24646448, Length=292, Percent_Identity=31.5068493150685, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24646452, Length=292, Percent_Identity=31.5068493150685, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24646450, Length=292, Percent_Identity=31.5068493150685, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI62472511, Length=292, Percent_Identity=31.5068493150685, Blast_Score=144, Evalue=6e-35,
Organism=Drosophila melanogaster, GI19921140, Length=295, Percent_Identity=30.5084745762712, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI28574286, Length=285, Percent_Identity=30.5263157894737, Blast_Score=124, Evalue=6e-29,
Organism=Drosophila melanogaster, GI28571528, Length=294, Percent_Identity=29.5918367346939, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24585516, Length=286, Percent_Identity=25.5244755244755, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI28574284, Length=282, Percent_Identity=28.0141843971631, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24585514, Length=285, Percent_Identity=27.719298245614, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI28574282, Length=285, Percent_Identity=27.719298245614, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI45552429, Length=282, Percent_Identity=28.0141843971631, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI45551003, Length=282, Percent_Identity=28.0141843971631, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: NA

Molecular weight: Translated: 34648; Mature: 34517

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKLKVVFLDRATIPSQIHLKPLSFEHQWVEYDFTSPEQVSERIEGADVVITNKVVLNES
CCCEEEEEEECCCCCCEEEEEECCCCCEEEEECCCCHHHHHHHCCCCCEEEEEEEEECCC
NLAQAQQLKLIAVSATGVNNVDVGYCKSNNIAVTNVQGYATQSVPEHVIAMLFTLKRNLV
CCCHHHEEEEEEEEECCCCCCEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
GYHQDIKAGEWQKDKQFCFFTHPIQDVAGSTLGLIGSGSLGQATAILAKSIGMNVIFAER
CCCCCCCCCCCCCCCCEEEEECCHHHHCCCCEEEEECCCCCHHHHHHHHHCCCEEEEECC
KEAGSCREGYLPFEAVLQQADAISLHCPLTEATRDLISERELTMMKSSAVLINAGRGGLV
CCCCCCCCCCCCHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHEEECCEEEEECCCCCCC
DEQALVEALKSNEIAGAGMDVFTQEPADNSNPLLANSHLPNLLLTPHVAWGSDSSIQKLS
CHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCEEECCCCCCEEECCEECCCCCCHHHHHH
DILIDNIDGFVAGNPQNLVI
HHHHHCCCCEEECCCCCCCC
>Mature Secondary Structure 
PKLKVVFLDRATIPSQIHLKPLSFEHQWVEYDFTSPEQVSERIEGADVVITNKVVLNES
CCEEEEEEECCCCCCEEEEEECCCCCEEEEECCCCHHHHHHHCCCCCEEEEEEEEECCC
NLAQAQQLKLIAVSATGVNNVDVGYCKSNNIAVTNVQGYATQSVPEHVIAMLFTLKRNLV
CCCHHHEEEEEEEEECCCCCCEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
GYHQDIKAGEWQKDKQFCFFTHPIQDVAGSTLGLIGSGSLGQATAILAKSIGMNVIFAER
CCCCCCCCCCCCCCCCEEEEECCHHHHCCCCEEEEECCCCCHHHHHHHHHCCCEEEEECC
KEAGSCREGYLPFEAVLQQADAISLHCPLTEATRDLISERELTMMKSSAVLINAGRGGLV
CCCCCCCCCCCCHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHEEECCEEEEECCCCCCC
DEQALVEALKSNEIAGAGMDVFTQEPADNSNPLLANSHLPNLLLTPHVAWGSDSSIQKLS
CHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCEEECCCCCCEEECCEECCCCCCHHHHHH
DILIDNIDGFVAGNPQNLVI
HHHHHCCCCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: hydroxypyruvate; NADPH; glycolate; NADP; gluconate; 2,5-diketo-D-gluconate; 2-keto-L-gulonate [C]

Specific reaction: hydroxypyruvate + NADPH = glycerate + NADP glycolate + NADP = NADPH + glyoxylate NADP + gluconate = NADPH + 2-Dehydro-D-gluconate 2,5-diketo-D-gluconate + NADPH = 5-ketogluconate + NADP 2-keto-L-gulonate + NADPH = L-idonate + NADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]