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The map label for this gene is miaA

Identifier: 218708322

GI number: 218708322

Start: 262015

End: 262947

Strand: Direct

Name: miaA

Synonym: VS_0269

Alternate gene names: 218708322

Gene position: 262015-262947 (Clockwise)

Preceding gene: 218708321

Following gene: 218708323

Centisome position: 7.94

GC content: 45.55

Gene sequence:

>933_bases
ATGACTGAAAAATTACCTTTAGCGTTGTTTTTAATGGGCCCCACGGCATCAGGAAAAACAGATTTAGCTATCCGCTTACG
TCAGAAATACCCGGTAGAGATCATCAGTGTCGATTCGGCGCTAATCTACAAAGACATGGATATTGGTACTGCTAAGCCGG
ATGCAGGAGAGCTCGCGCTCGCGCCTCATCGCTTGATTGATATTCTCGACCCAAGCGAAGCGTATTCTGCGGCTGACTTT
CGTCGTGATGCGATCAGTGAAATGAATAAGATTGTAGCGGAAGGTAAAATCCCGCTACTTGTTGGTGGCACAATGCTTTA
CTACAAGGCATTGTTGGAAGGTTTATCGCCACTACCCGCTGCGGATCAAGAGATTCGTAAGCAGATTGAAGCGGAGTCTA
TTGAGCAGGGTTGGCAGGCATTGCACGATCAATTAAGAGAGATAGATCCCGTATCCGCTGAAAGAATACACCCAAATGAT
CCACAAAGGCTTTCAAGGGCATTGGAAGTTTATCGAATTTCGGGTAAAACATTAACAGAGCTAACTCAAACGAAAGGCGA
TAGCCTGCCATTTCGTGTAAAACAATTTGCAATAGCTCCCAAGGAAAGGAAAGAACTCCATCGCCGCATTGAGCTGCGTT
TCGAGAAGATGATAGAAGCGGGATTTGAAGATGAAATGAAAGCGTTGTACGCCAGAGAAGATCTTCACCCTGAACTGCCA
TCGATCCGATGCGTTGGTTATAGGCAGATGTGGGATTATTTAGATGGGAATTGCGATTTAGACGAAGCGGTTTTCCGTGG
TGTCTGCGCAACTCGTCAATTGGCCAAGCGACAAATCACCTGGTTACGCAGTTGGGATGATTTAACTTGGTTGGATAGCG
AAAACATTGAACAGGCGTTAGAAACTCTTTCAGATGCAATAGCATCTGATTAG

Upstream 100 bases:

>100_bases
GATTTAACTGTTGCGATGAGGCTTTTCTTAGTAGACAGGTGTATCGCGACATGAAGCCTTTTAATTAAGTGCTCTAACAG
AGAAATAAATACAAAATATC

Downstream 100 bases:

>100_bases
TATTGCTGTGTATAATGTGATCGCTTTTGCGTGAATATACTCTGGAAAGGATCAGTTGTTGAGGCTTTTAAAAATCACAG
CCTTTCAATAACAACGGAGT

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MTEKLPLALFLMGPTASGKTDLAIRLRQKYPVEIISVDSALIYKDMDIGTAKPDAGELALAPHRLIDILDPSEAYSAADF
RRDAISEMNKIVAEGKIPLLVGGTMLYYKALLEGLSPLPAADQEIRKQIEAESIEQGWQALHDQLREIDPVSAERIHPND
PQRLSRALEVYRISGKTLTELTQTKGDSLPFRVKQFAIAPKERKELHRRIELRFEKMIEAGFEDEMKALYAREDLHPELP
SIRCVGYRQMWDYLDGNCDLDEAVFRGVCATRQLAKRQITWLRSWDDLTWLDSENIEQALETLSDAIASD

Sequences:

>Translated_310_residues
MTEKLPLALFLMGPTASGKTDLAIRLRQKYPVEIISVDSALIYKDMDIGTAKPDAGELALAPHRLIDILDPSEAYSAADF
RRDAISEMNKIVAEGKIPLLVGGTMLYYKALLEGLSPLPAADQEIRKQIEAESIEQGWQALHDQLREIDPVSAERIHPND
PQRLSRALEVYRISGKTLTELTQTKGDSLPFRVKQFAIAPKERKELHRRIELRFEKMIEAGFEDEMKALYAREDLHPELP
SIRCVGYRQMWDYLDGNCDLDEAVFRGVCATRQLAKRQITWLRSWDDLTWLDSENIEQALETLSDAIASD
>Mature_309_residues
TEKLPLALFLMGPTASGKTDLAIRLRQKYPVEIISVDSALIYKDMDIGTAKPDAGELALAPHRLIDILDPSEAYSAADFR
RDAISEMNKIVAEGKIPLLVGGTMLYYKALLEGLSPLPAADQEIRKQIEAESIEQGWQALHDQLREIDPVSAERIHPNDP
QRLSRALEVYRISGKTLTELTQTKGDSLPFRVKQFAIAPKERKELHRRIELRFEKMIEAGFEDEMKALYAREDLHPELPS
IRCVGYRQMWDYLDGNCDLDEAVFRGVCATRQLAKRQITWLRSWDDLTWLDSENIEQALETLSDAIASD

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=312, Percent_Identity=27.8846153846154, Blast_Score=115, Evalue=5e-26,
Organism=Escherichia coli, GI1790613, Length=296, Percent_Identity=72.6351351351351, Blast_Score=453, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI25144712, Length=302, Percent_Identity=29.4701986754967, Blast_Score=117, Evalue=7e-27,
Organism=Saccharomyces cerevisiae, GI6324848, Length=289, Percent_Identity=30.4498269896194, Blast_Score=129, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24649830, Length=276, Percent_Identity=30.0724637681159, Blast_Score=118, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_VIBSL (B7VI54)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002415943.1
- ProteinModelPortal:   B7VI54
- SMR:   B7VI54
- GeneID:   7159920
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_0269
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   PRK00091
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 35181; Mature: 35049

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEKLPLALFLMGPTASGKTDLAIRLRQKYPVEIISVDSALIYKDMDIGTAKPDAGELAL
CCCCCCEEEEEECCCCCCCCCEEEEECCCCCEEEEECCCCCEEECCCCCCCCCCCCCEEE
APHRLIDILDPSEAYSAADFRRDAISEMNKIVAEGKIPLLVGGTMLYYKALLEGLSPLPA
CHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHCCCCCCC
ADQEIRKQIEAESIEQGWQALHDQLREIDPVSAERIHPNDPQRLSRALEVYRISGKTLTE
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHH
LTQTKGDSLPFRVKQFAIAPKERKELHRRIELRFEKMIEAGFEDEMKALYAREDLHPELP
HHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCC
SIRCVGYRQMWDYLDGNCDLDEAVFRGVCATRQLAKRQITWLRSWDDLTWLDSENIEQAL
CEEEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
ETLSDAIASD
HHHHHHHCCC
>Mature Secondary Structure 
TEKLPLALFLMGPTASGKTDLAIRLRQKYPVEIISVDSALIYKDMDIGTAKPDAGELAL
CCCCCEEEEEECCCCCCCCCEEEEECCCCCEEEEECCCCCEEECCCCCCCCCCCCCEEE
APHRLIDILDPSEAYSAADFRRDAISEMNKIVAEGKIPLLVGGTMLYYKALLEGLSPLPA
CHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHCCCCCCC
ADQEIRKQIEAESIEQGWQALHDQLREIDPVSAERIHPNDPQRLSRALEVYRISGKTLTE
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHH
LTQTKGDSLPFRVKQFAIAPKERKELHRRIELRFEKMIEAGFEDEMKALYAREDLHPELP
HHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCC
SIRCVGYRQMWDYLDGNCDLDEAVFRGVCATRQLAKRQITWLRSWDDLTWLDSENIEQAL
CEEEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
ETLSDAIASD
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA