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The map label for this gene is amiB [H]

Identifier: 218708320

GI number: 218708320

Start: 257871

End: 259586

Strand: Direct

Name: amiB [H]

Synonym: VS_0267

Alternate gene names: 218708320

Gene position: 257871-259586 (Clockwise)

Preceding gene: 218708319

Following gene: 218708321

Centisome position: 7.82

GC content: 44.81

Gene sequence:

>1716_bases
ATGTTGATTTCTAGACGCCTTATTTCAGCAGTAGCTATGTTGGCTGCTGTTTTTTCTATACTGTTTTCTTCACTTGTTTC
TGCAAACTCACTAAAGAGTTTAAGGGTTTGGCCTTCTCCAGAAGAAACTCGTGTGGTTATCGATTTAAAATCAGAAGCGG
ATTTCAGCTATTTCACCCTGAGTAGCCCGAGTCGCTTGGTTGTTGATTTAAAAAAGACCAACCTTGCAACTAAGTTACCG
GTCGTGGTGAAAGACAGCCCCGTTTTATCTAAGATTCGTAAAAGCTCACCGCCAGACAAAAACACCTATCGCTTGGTTTT
TGAGTTAAAGAAGTCGTCTAAAGCAGAGTTGTTTAAACTCAGTCCGACTCCTGGTGGTCAGTACGGACATCGCTTGGTGA
TCGATTTGCCTCATGGTGCGGCAAGTAAAGGCAAAGCAACATCAACGCCGAGTAAGCCAACAGTGAGTAAGAACCTCAAT
CAAGTTAAGCGTCAGCGTGATGTTCTGATTGTGATTGACCCAGGCCACGGTGGTGAAGATCCTGGTTCCATTGGCCCATC
TCGTAAATATGAGAAGAATGCGACTCTTAGTATTTCGAAAAAGATCGCTGCGCAGTTGAATGCAGTTCCGGGAATCAAAA
CGCGTTTAACACGTAATGCTGATTACTTTGTAAACCTGAACAGACGTGTTGCAATTGCTCGTGAGAACGAAGCACACCTC
TTGATCTCTATTCACGCCGATGCCTTTACGACGCCTCAACCAAGAGGTGGTTCGGTGTTCGTGTTGAATACTCGACGGGC
AAATACTGAGATTTCCCGTTGGATTGAGAACAAAGAGAAACAGTCAGAGTTGCTGGGCGGCAGCGGTGCTGCGTTTACTG
GCAATATTGCAGATAAAAACGTCAACCAAACCCTGCTTGATTTGCAGTTTAGCCACTCTCAAAAAGAGGGCTATAAACTG
GCAACGACTATTCTCTCTGAAATGGGTAAGGTCGCAAAACTGCATAACAGTAAGCCGATTAACACCAGCTTGGCAGTGTT
ACGTTCACCACAAATTCCATCGGTACTTGTAGAAACGGGCTTTATTTCGAATCCGACCGAAGAGAAGCTTCTTTTCCAAC
GTTCGCATCAAGACAAACTTGCGCGAGCTGTGACCAAAGCGGTCGTTAAATATTTGAAAGCGAATCCACCAGAAGGGATT
GTTATTTCTAACGCGGCGGCATCATCTAACTCTACCAGTCAGCACAAAGTACAGCGTGGTGAATCATTGTCTGTGATTGC
GAAGAGGTACGGCACGTCTACACAAGCATTAGTGAAGTTGAATGGTTTGAAGTCGACGAGCTTAGCGGTAGGGCAAATTC
TAAAAATTCCAAGCTCGGGTTCACAAGCGGCTACCACAACAAGCCGTAAAACGCTGACACACACGGTTAAGTCTGGAGAG
TACTTAGGCAAGATAGCTAGCCGTTACAAAGTATCGGTAGCAAGCATTAAACGTGAGAATAGCTTGAAATCAGAAACCGT
GAGAGTAGGCCAAAAGTTACGTATTACGGTTGAAGTAAAAGATATACCTCTACGTAAACATAAAGTCGCAAGAGGCGATT
ATCTTGGCAAGATTGCTTCGAAATATGGCGTGAGCGTCAACAGTATCCGTCAAGCAAACAGACTACGTTCCGATGAGTTG
GCCATTGGCCAAGTGCTGATTATTCCGCACAAATAG

Upstream 100 bases:

>100_bases
CAGATTTGGATATTGATATTCGTTACCAAGACGATCACCGCATTGTTTCTTTAACCGCTAACAGTGAATACGGACAGCGC
TTACTTAGTCAGTTGGAGTT

Downstream 100 bases:

>100_bases
TTGGCTAATGTTGAAGTAATGTTTAGAACATAGAAGCAATCTCAAACGCGAGAAGTGCTTCTATAAATGGCAGTAAGGTA
GCTATACATATGACGATCAA

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 571; Mature: 571

Protein sequence:

>571_residues
MLISRRLISAVAMLAAVFSILFSSLVSANSLKSLRVWPSPEETRVVIDLKSEADFSYFTLSSPSRLVVDLKKTNLATKLP
VVVKDSPVLSKIRKSSPPDKNTYRLVFELKKSSKAELFKLSPTPGGQYGHRLVIDLPHGAASKGKATSTPSKPTVSKNLN
QVKRQRDVLIVIDPGHGGEDPGSIGPSRKYEKNATLSISKKIAAQLNAVPGIKTRLTRNADYFVNLNRRVAIARENEAHL
LISIHADAFTTPQPRGGSVFVLNTRRANTEISRWIENKEKQSELLGGSGAAFTGNIADKNVNQTLLDLQFSHSQKEGYKL
ATTILSEMGKVAKLHNSKPINTSLAVLRSPQIPSVLVETGFISNPTEEKLLFQRSHQDKLARAVTKAVVKYLKANPPEGI
VISNAAASSNSTSQHKVQRGESLSVIAKRYGTSTQALVKLNGLKSTSLAVGQILKIPSSGSQAATTTSRKTLTHTVKSGE
YLGKIASRYKVSVASIKRENSLKSETVRVGQKLRITVEVKDIPLRKHKVARGDYLGKIASKYGVSVNSIRQANRLRSDEL
AIGQVLIIPHK

Sequences:

>Translated_571_residues
MLISRRLISAVAMLAAVFSILFSSLVSANSLKSLRVWPSPEETRVVIDLKSEADFSYFTLSSPSRLVVDLKKTNLATKLP
VVVKDSPVLSKIRKSSPPDKNTYRLVFELKKSSKAELFKLSPTPGGQYGHRLVIDLPHGAASKGKATSTPSKPTVSKNLN
QVKRQRDVLIVIDPGHGGEDPGSIGPSRKYEKNATLSISKKIAAQLNAVPGIKTRLTRNADYFVNLNRRVAIARENEAHL
LISIHADAFTTPQPRGGSVFVLNTRRANTEISRWIENKEKQSELLGGSGAAFTGNIADKNVNQTLLDLQFSHSQKEGYKL
ATTILSEMGKVAKLHNSKPINTSLAVLRSPQIPSVLVETGFISNPTEEKLLFQRSHQDKLARAVTKAVVKYLKANPPEGI
VISNAAASSNSTSQHKVQRGESLSVIAKRYGTSTQALVKLNGLKSTSLAVGQILKIPSSGSQAATTTSRKTLTHTVKSGE
YLGKIASRYKVSVASIKRENSLKSETVRVGQKLRITVEVKDIPLRKHKVARGDYLGKIASKYGVSVNSIRQANRLRSDEL
AIGQVLIIPHK
>Mature_571_residues
MLISRRLISAVAMLAAVFSILFSSLVSANSLKSLRVWPSPEETRVVIDLKSEADFSYFTLSSPSRLVVDLKKTNLATKLP
VVVKDSPVLSKIRKSSPPDKNTYRLVFELKKSSKAELFKLSPTPGGQYGHRLVIDLPHGAASKGKATSTPSKPTVSKNLN
QVKRQRDVLIVIDPGHGGEDPGSIGPSRKYEKNATLSISKKIAAQLNAVPGIKTRLTRNADYFVNLNRRVAIARENEAHL
LISIHADAFTTPQPRGGSVFVLNTRRANTEISRWIENKEKQSELLGGSGAAFTGNIADKNVNQTLLDLQFSHSQKEGYKL
ATTILSEMGKVAKLHNSKPINTSLAVLRSPQIPSVLVETGFISNPTEEKLLFQRSHQDKLARAVTKAVVKYLKANPPEGI
VISNAAASSNSTSQHKVQRGESLSVIAKRYGTSTQALVKLNGLKSTSLAVGQILKIPSSGSQAATTTSRKTLTHTVKSGE
YLGKIASRYKVSVASIKRENSLKSETVRVGQKLRITVEVKDIPLRKHKVARGDYLGKIASKYGVSVNSIRQANRLRSDEL
AIGQVLIIPHK

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1790611, Length=424, Percent_Identity=35.8490566037736, Blast_Score=260, Evalue=2e-70,
Organism=Escherichia coli, GI87082163, Length=408, Percent_Identity=33.3333333333333, Blast_Score=228, Evalue=6e-61,
Organism=Escherichia coli, GI1788776, Length=224, Percent_Identity=38.8392857142857, Blast_Score=160, Evalue=3e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 62175; Mature: 62175

Theoretical pI: Translated: 11.03; Mature: 11.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLISRRLISAVAMLAAVFSILFSSLVSANSLKSLRVWPSPEETRVVIDLKSEADFSYFTL
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCEEEEEECCCCCCEEEEE
SSPSRLVVDLKKTNLATKLPVVVKDSPVLSKIRKSSPPDKNTYRLVFELKKSSKAELFKL
CCCCEEEEEEECCCCCEECCEEEECCHHHHHHHHCCCCCCCCEEEEEEECCCCCCEEEEE
SPTPGGQYGHRLVIDLPHGAASKGKATSTPSKPTVSKNLNQVKRQRDVLIVIDPGHGGED
CCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEEECCCCCCC
PGSIGPSRKYEKNATLSISKKIAAQLNAVPGIKTRLTRNADYFVNLNRRVAIARENEAHL
CCCCCCCCCCCCCCEEEHHHHHHHHHCCCCCCHHHEECCCCEEEEECCEEEEEECCCCEE
LISIHADAFTTPQPRGGSVFVLNTRRANTEISRWIENKEKQSELLGGSGAAFTGNIADKN
EEEEECCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCC
VNQTLLDLQFSHSQKEGYKLATTILSEMGKVAKLHNSKPINTSLAVLRSPQIPSVLVETG
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHEEECCCCCEEEEECC
FISNPTEEKLLFQRSHQDKLARAVTKAVVKYLKANPPEGIVISNAAASSNSTSQHKVQRG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHCC
ESLSVIAKRYGTSTQALVKLNGLKSTSLAVGQILKIPSSGSQAATTTSRKTLTHTVKSGE
CHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHEECCCCCCCCCCCCCCHHHHHHHHCCH
YLGKIASRYKVSVASIKRENSLKSETVRVGQKLRITVEVKDIPLRKHKVARGDYLGKIAS
HHHHHHHHHEEHHHHHHHHCCHHHHHHHCCCEEEEEEEEECCCCHHHHHHCCHHHHHHHH
KYGVSVNSIRQANRLRSDELAIGQVLIIPHK
HHCCCHHHHHHHHHCCCCCEEECEEEEEECC
>Mature Secondary Structure
MLISRRLISAVAMLAAVFSILFSSLVSANSLKSLRVWPSPEETRVVIDLKSEADFSYFTL
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCEEEEEECCCCCCEEEEE
SSPSRLVVDLKKTNLATKLPVVVKDSPVLSKIRKSSPPDKNTYRLVFELKKSSKAELFKL
CCCCEEEEEEECCCCCEECCEEEECCHHHHHHHHCCCCCCCCEEEEEEECCCCCCEEEEE
SPTPGGQYGHRLVIDLPHGAASKGKATSTPSKPTVSKNLNQVKRQRDVLIVIDPGHGGED
CCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEEECCCCCCC
PGSIGPSRKYEKNATLSISKKIAAQLNAVPGIKTRLTRNADYFVNLNRRVAIARENEAHL
CCCCCCCCCCCCCCEEEHHHHHHHHHCCCCCCHHHEECCCCEEEEECCEEEEEECCCCEE
LISIHADAFTTPQPRGGSVFVLNTRRANTEISRWIENKEKQSELLGGSGAAFTGNIADKN
EEEEECCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCC
VNQTLLDLQFSHSQKEGYKLATTILSEMGKVAKLHNSKPINTSLAVLRSPQIPSVLVETG
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHEEECCCCCEEEEECC
FISNPTEEKLLFQRSHQDKLARAVTKAVVKYLKANPPEGIVISNAAASSNSTSQHKVQRG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHCC
ESLSVIAKRYGTSTQALVKLNGLKSTSLAVGQILKIPSSGSQAATTTSRKTLTHTVKSGE
CHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHEECCCCCCCCCCCCCCHHHHHHHHCCH
YLGKIASRYKVSVASIKRENSLKSETVRVGQKLRITVEVKDIPLRKHKVARGDYLGKIAS
HHHHHHHHHEEHHHHHHHHCCHHHHHHHCCCEEEEEEEEECCCCHHHHHHCCHHHHHHHH
KYGVSVNSIRQANRLRSDELAIGQVLIIPHK
HHCCCHHHHHHHHHCCCCCEEECEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609; 2676972 [H]