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The map label for this gene is kdsB [H]

Identifier: 218708280

GI number: 218708280

Start: 211999

End: 212751

Strand: Direct

Name: kdsB [H]

Synonym: VS_0218

Alternate gene names: 218708280

Gene position: 211999-212751 (Clockwise)

Preceding gene: 218708279

Following gene: 218708281

Centisome position: 6.43

GC content: 40.64

Gene sequence:

>753_bases
ATGGGGGTGAAAGTTGTAATTCCAGCGCGATATGGCTCATCTAGGTTGCCTGGTAAACCGTTATTAACTTTACTTGATAA
ACCTGTTATTTGGCATGTAATCGAGCGTTGCAAAGAGGCGGGCATCGAGCAATCTGATATTTTTGTCGCTACTGATGACC
AAAGAATTAGTGGTGCGTTGAAAGATGAGGGTATCCAAGTTGTTCTAACATCTTCGTTGCATCAATCTGGTACGGATCGT
ATAAATGAGGTTGTTCAGATCAAAGAGTGGAGTGGTGATATCGTTGTAGTCAATGTGCAAGGAGATGAGCCTATGATCCC
TCCAGGGCTCATTCAACAGCTTGTATCATTTACGTTGTCAAACCCGTCTTACCATCTGACTACAGCAGTAGTTCCTTTGC
TTTCCCAAGATGACTTCATAAACCCAAATGTAGTTAAAGCTATTATTGGTCATAATGGCCGAGCGCTTTATTTTACTCGC
TCCGCATCGCCAATGAATCGCGATAACCCTTCTGATTTGTCATTTGCAAAAAGGCATATCGGGATTTATGCCTACCGAGT
TTCTGCTTTGAAACAATTCTGTTCATATGTAGAGGATGAACTTGAGAGTTATGAAAAATTGGAACAGTTACGTGCTTTAA
GCCATGGAATGAGTATAGGTGCAATGACATTTGATGGGATCGTCCCTCATGGAATAGATACTAATGATGATTACGAAACT
ATCAAAAAAATAATGGAAGAGAAGGCGTTTTAA

Upstream 100 bases:

>100_bases
ATTGTTGGTAACGCAACTTGATAAGCCACTAAAAGAGGTCTACGAGCCATTACTTAACAAAATCCGTTTAGATGATGTAG
CAACAATGGAGCAATAAAGT

Downstream 100 bases:

>100_bases
ATGGCTAGTCCAATTGATATTGCAAGAGAAGTAATTCAGACCGAAATAGAAGGGTTGGATTACATGGCAAAAAGGTTAGG
CTCTGAATTTGAAGTGGCTG

Product: 3-deoxy-manno-octulosonate cytidylyltransferase

Products: NA

Alternate protein names: CMP-2-keto-3-deoxyoctulosonic acid synthase; CKS; CMP-KDO synthase [H]

Number of amino acids: Translated: 250; Mature: 249

Protein sequence:

>250_residues
MGVKVVIPARYGSSRLPGKPLLTLLDKPVIWHVIERCKEAGIEQSDIFVATDDQRISGALKDEGIQVVLTSSLHQSGTDR
INEVVQIKEWSGDIVVVNVQGDEPMIPPGLIQQLVSFTLSNPSYHLTTAVVPLLSQDDFINPNVVKAIIGHNGRALYFTR
SASPMNRDNPSDLSFAKRHIGIYAYRVSALKQFCSYVEDELESYEKLEQLRALSHGMSIGAMTFDGIVPHGIDTNDDYET
IKKIMEEKAF

Sequences:

>Translated_250_residues
MGVKVVIPARYGSSRLPGKPLLTLLDKPVIWHVIERCKEAGIEQSDIFVATDDQRISGALKDEGIQVVLTSSLHQSGTDR
INEVVQIKEWSGDIVVVNVQGDEPMIPPGLIQQLVSFTLSNPSYHLTTAVVPLLSQDDFINPNVVKAIIGHNGRALYFTR
SASPMNRDNPSDLSFAKRHIGIYAYRVSALKQFCSYVEDELESYEKLEQLRALSHGMSIGAMTFDGIVPHGIDTNDDYET
IKKIMEEKAF
>Mature_249_residues
GVKVVIPARYGSSRLPGKPLLTLLDKPVIWHVIERCKEAGIEQSDIFVATDDQRISGALKDEGIQVVLTSSLHQSGTDRI
NEVVQIKEWSGDIVVVNVQGDEPMIPPGLIQQLVSFTLSNPSYHLTTAVVPLLSQDDFINPNVVKAIIGHNGRALYFTRS
ASPMNRDNPSDLSFAKRHIGIYAYRVSALKQFCSYVEDELESYEKLEQLRALSHGMSIGAMTFDGIVPHGIDTNDDYETI
KKIMEEKAF

Specific function: Activates KDO (a required 8-carbon sugar) for incorporation into bacterial lipopolysaccharide in Gram-negative bacteria [H]

COG id: COG1212

COG function: function code M; CMP-2-keto-3-deoxyoctulosonic acid synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the kdsB family [H]

Homologues:

Organism=Escherichia coli, GI1787147, Length=255, Percent_Identity=40.7843137254902, Blast_Score=188, Evalue=4e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003329
- InterPro:   IPR004528 [H]

Pfam domain/function: PF02348 CTP_transf_3 [H]

EC number: =2.7.7.38 [H]

Molecular weight: Translated: 27756; Mature: 27625

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVKVVIPARYGSSRLPGKPLLTLLDKPVIWHVIERCKEAGIEQSDIFVATDDQRISGAL
CCEEEEEECCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHCCCCCCCEEEEECCHHHHCCC
KDEGIQVVLTSSLHQSGTDRINEVVQIKEWSGDIVVVNVQGDEPMIPPGLIQQLVSFTLS
CCCCEEEEEECHHHHCCHHHHHHHEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHCC
NPSYHLTTAVVPLLSQDDFINPNVVKAIIGHNGRALYFTRSASPMNRDNPSDLSFAKRHI
CCCEEHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHH
GIYAYRVSALKQFCSYVEDELESYEKLEQLRALSHGMSIGAMTFDGIVPHGIDTNDDYET
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCHHH
IKKIMEEKAF
HHHHHHHCCC
>Mature Secondary Structure 
GVKVVIPARYGSSRLPGKPLLTLLDKPVIWHVIERCKEAGIEQSDIFVATDDQRISGAL
CEEEEEECCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHCCCCCCCEEEEECCHHHHCCC
KDEGIQVVLTSSLHQSGTDRINEVVQIKEWSGDIVVVNVQGDEPMIPPGLIQQLVSFTLS
CCCCEEEEEECHHHHCCHHHHHHHEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHCC
NPSYHLTTAVVPLLSQDDFINPNVVKAIIGHNGRALYFTRSASPMNRDNPSDLSFAKRHI
CCCEEHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHH
GIYAYRVSALKQFCSYVEDELESYEKLEQLRALSHGMSIGAMTFDGIVPHGIDTNDDYET
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCHHH
IKKIMEEKAF
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA