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The map label for this gene is WaaN [H]

Identifier: 218708262

GI number: 218708262

Start: 193561

End: 194514

Strand: Reverse

Name: WaaN [H]

Synonym: VS_0199

Alternate gene names: 218708262

Gene position: 194514-193561 (Counterclockwise)

Preceding gene: 218708263

Following gene: 218708255

Centisome position: 5.9

GC content: 43.82

Gene sequence:

>954_bases
GTGACAACTCAACGCGACGACTACGATCCTAAAGCATACAACCCCAAATTCATCAGTGCGTTTTTATCACCAAAACACTG
GGGAACTTGGCTCGGGCTATGCCTATGTCTACCCATTTCTTTGCTGCCAAACTCGATTCGAGTAGCCATAGCAAAATTCG
TGGCGACAAAAATGGCGAAGAAGCAACGTGGCTCAGTACAAAAGGCACGTATAAACCTCGCGCTTTGTTTTCCAGAAAAG
TCTGTTCAAGAGCGTGAAACCATTCTAACCAATTGCCTAACAACTGCTGGCGCCTTTTTACTTGGTTTTCCTGCCATATC
ATTACGCAGTAAACGTTGGCTTGAAAAACACTCAGAAATCATTGGATTAGAACACTTACAACAGCTACAAAAAGACAATC
AAAGTGCCATTTTGCTCGTACCTCACTCTTGGTGCATCGACATTCCGGCTATCCTTTTAGCTTCAAGAGGCTTACCCGTT
TCTGCGATGGCAAATAGCCAGAAAAACCCTCTGACCGATTGGCTCATGCACAAGCAAAGAGTTCAGTATGGCGGACGAGT
TTATGATCGCAGTGGAGGCATTAAGCCATTTATCAAATCAGTCAAAGATGGCTACTTGGGATATTACCTGCCAGATCAAG
ACCATGGCCCAGAGCAAAGTGTATTCTCAGATTTCTTTGCGACTGAAAAAGCAACCTTGCCCGGACTTGGAAAACTAGCA
AAAGTAAGCCGAGCGAAGATCGTTCCAACCTTTGCCAGCTATAATATTGAGACGGGTAAATACGAAATTGAAATCAAAGC
ACCCATTGAGTTATCCGGCGATGAACATCAAGACGCTAGGGCAATGAATGAAGTCGTTGAAGGTTTTGTTAATCCGAAAC
CTGAGCAGTACATGTGGATTTTAAAGTTGTTAAAAACACGCAGGAATGGACAAGACCCATATGTGGGGTATTAA

Upstream 100 bases:

>100_bases
GTACATGCAGTCAGTTAATCGCTAGATTACACTCTCCCGAAAACAAGCTAACGGTATACTGTTAGCTTGTTCAATATATA
TCGCCAGTTAAAGGTTTACA

Downstream 100 bases:

>100_bases
CCTACATATGGGTATAAACCTACACGTAGGCATATAGACCCGATTGATTAGTTAGAAGTTTCTAACTAATCAATAACTTA
AAATCTTTGATATTAATAAA

Product: lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MTTQRDDYDPKAYNPKFISAFLSPKHWGTWLGLCLCLPISLLPNSIRVAIAKFVATKMAKKQRGSVQKARINLALCFPEK
SVQERETILTNCLTTAGAFLLGFPAISLRSKRWLEKHSEIIGLEHLQQLQKDNQSAILLVPHSWCIDIPAILLASRGLPV
SAMANSQKNPLTDWLMHKQRVQYGGRVYDRSGGIKPFIKSVKDGYLGYYLPDQDHGPEQSVFSDFFATEKATLPGLGKLA
KVSRAKIVPTFASYNIETGKYEIEIKAPIELSGDEHQDARAMNEVVEGFVNPKPEQYMWILKLLKTRRNGQDPYVGY

Sequences:

>Translated_317_residues
MTTQRDDYDPKAYNPKFISAFLSPKHWGTWLGLCLCLPISLLPNSIRVAIAKFVATKMAKKQRGSVQKARINLALCFPEK
SVQERETILTNCLTTAGAFLLGFPAISLRSKRWLEKHSEIIGLEHLQQLQKDNQSAILLVPHSWCIDIPAILLASRGLPV
SAMANSQKNPLTDWLMHKQRVQYGGRVYDRSGGIKPFIKSVKDGYLGYYLPDQDHGPEQSVFSDFFATEKATLPGLGKLA
KVSRAKIVPTFASYNIETGKYEIEIKAPIELSGDEHQDARAMNEVVEGFVNPKPEQYMWILKLLKTRRNGQDPYVGY
>Mature_316_residues
TTQRDDYDPKAYNPKFISAFLSPKHWGTWLGLCLCLPISLLPNSIRVAIAKFVATKMAKKQRGSVQKARINLALCFPEKS
VQERETILTNCLTTAGAFLLGFPAISLRSKRWLEKHSEIIGLEHLQQLQKDNQSAILLVPHSWCIDIPAILLASRGLPVS
AMANSQKNPLTDWLMHKQRVQYGGRVYDRSGGIKPFIKSVKDGYLGYYLPDQDHGPEQSVFSDFFATEKATLPGLGKLAK
VSRAKIVPTFASYNIETGKYEIEIKAPIELSGDEHQDARAMNEVVEGFVNPKPEQYMWILKLLKTRRNGQDPYVGY

Specific function: Transfers myristate or laurate, activated on ACP, to (KDO)2-(lauroyl)-lipid IVA [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1788161, Length=317, Percent_Identity=44.1640378548896, Blast_Score=286, Evalue=1e-78,
Organism=Escherichia coli, GI1787292, Length=300, Percent_Identity=31.3333333333333, Blast_Score=141, Evalue=5e-35,
Organism=Escherichia coli, GI87082094, Length=304, Percent_Identity=28.2894736842105, Blast_Score=119, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011921 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.-

Molecular weight: Translated: 35681; Mature: 35550

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQRDDYDPKAYNPKFISAFLSPKHWGTWLGLCLCLPISLLPNSIRVAIAKFVATKMAK
CCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
KQRGSVQKARINLALCFPEKSVQERETILTNCLTTAGAFLLGFPAISLRSKRWLEKHSEI
HHHCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
IGLEHLQQLQKDNQSAILLVPHSWCIDIPAILLASRGLPVSAMANSQKNPLTDWLMHKQR
HHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHCCCCCCHHHCCCCCCCHHHHHHHHHH
VQYGGRVYDRSGGIKPFIKSVKDGYLGYYLPDQDHGPEQSVFSDFFATEKATLPGLGKLA
HHHCCEEEECCCCHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCHHHH
KVSRAKIVPTFASYNIETGKYEIEIKAPIELSGDEHQDARAMNEVVEGFVNPKPEQYMWI
HHHHHHEEEEHHCCCEECCEEEEEEECCEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHH
LKLLKTRRNGQDPYVGY
HHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TTQRDDYDPKAYNPKFISAFLSPKHWGTWLGLCLCLPISLLPNSIRVAIAKFVATKMAK
CCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
KQRGSVQKARINLALCFPEKSVQERETILTNCLTTAGAFLLGFPAISLRSKRWLEKHSEI
HHHCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
IGLEHLQQLQKDNQSAILLVPHSWCIDIPAILLASRGLPVSAMANSQKNPLTDWLMHKQR
HHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHCCCCCCHHHCCCCCCCHHHHHHHHHH
VQYGGRVYDRSGGIKPFIKSVKDGYLGYYLPDQDHGPEQSVFSDFFATEKATLPGLGKLA
HHHCCEEEECCCCHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCHHHH
KVSRAKIVPTFASYNIETGKYEIEIKAPIELSGDEHQDARAMNEVVEGFVNPKPEQYMWI
HHHHHHEEEEHHCCCEECCEEEEEEECCEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHH
LKLLKTRRNGQDPYVGY
HHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]