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The map label for this gene is gbsB [H]

Identifier: 218708234

GI number: 218708234

Start: 168488

End: 169648

Strand: Reverse

Name: gbsB [H]

Synonym: VS_0171

Alternate gene names: 218708234

Gene position: 169648-168488 (Counterclockwise)

Preceding gene: 218708235

Following gene: 218708233

Centisome position: 5.14

GC content: 46.08

Gene sequence:

>1161_bases
ATGACACAGTTTCAACACTATCAACCGACCAAGCTAACCTTTGGTGCGGGTGAGATTCAGAAAATAGGTCAACTTATCGC
TCAATATGGCACACGTTGCCTAGTAGTATCCGAACCTATTTTTGATGCAGTAAAGCCAGCCTATGACCGCATTTTTACTT
TGCTTTCAGAGCAAGGAATTGAAGTGACTCATTTTGATGGTGTCGTACCGAATCCGCCTACAACCGTCGTTGAACTTGGC
CGCCAAGTGGCCATCGGAAATAACTGTGATGTGGTTTTGGCCATTGGTGGTGGTTCATCGATTGATACAGCAAAAATCAT
TTCGGCAACGATCAATACAGAGTCTCTAGATTGGGCTCATTGGTTTGCCACTTATGATTCACCGTTTGGGGACGTCAACC
CACTACCCGCGAAAGTCGTACCTTTAATCGCAGTACCTACAACATCGGGAACTGGCTCTCAAGTGACGCAAGCGGCAGTG
ATTACCGACCTTGAGCAACATGCAAAGCTAACACTGTTTCACCCTGAATTTTTCCCTTGTGAAGCGTTAATCGATCCAGA
GCTCATGCTGACACTGCCTCCACGTATGACAGCTATGACAGGATTTGATGCTTTTTCACATGCGTTTGAATCATTTACGG
GTACTCGACCATCTCCATTTGTTGATGGTATGGCGTTAGAAGCAATGAAGCTTGTCATTGATCACCTACCGAGTGTTGTC
GACAACGGCGCAGATTTACATGGCCGCTGCCAACTCGCGAAAGCAGACACACTTGGCGGGATCTCGCTAGCTAATGGCGG
TGCGGGGGCACCACACCCTTTAGGCGAAATCTTAGGTAGTAGCAAAACAAACCTACCACATGGGTTAACACTCGCAGTGG
TTTATCCAGCTTATGCACAACTTCAATGGCGCAAGCAACCAGAGAGATTTGCTCAAGTGGCTGAGTTATTTGGTGCTGTG
GGAAGTACCGAAGAAAAAGCTCAATCATTAGCGGCTGCTCTTGTGAAATTCCTTGAACGTATCGGTCTTGAATCGAACTT
AACTCAAGTAGGTGTCACCAAAGAAGATGTTCAAAAGTTGGAGCCGGCTTTTTGCTTTGATCTACCACTAACCAGTGGTG
AAGAAATGAAAAAAGTATTGCAGGCCAGTTTGGTTGAATAA

Upstream 100 bases:

>100_bases
AGTTTCTATCGAATCGATGCGCCACAGCAATCTAGAAATAACGGTGCTAAACCTACCCCCCTCTATGCTCGGGTTTGGCA
CCACTACCAAGGATTAAACC

Downstream 100 bases:

>100_bases
GCGACAGTCACAAATTCACGTTTGTCATAATTAAAGAAAATGGAGATTGAAACATGGAATGCAAGAACAACACTTTAGGT
CATACAGCTTTGAAATTGGA

Product: putative alcohol dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MTQFQHYQPTKLTFGAGEIQKIGQLIAQYGTRCLVVSEPIFDAVKPAYDRIFTLLSEQGIEVTHFDGVVPNPPTTVVELG
RQVAIGNNCDVVLAIGGGSSIDTAKIISATINTESLDWAHWFATYDSPFGDVNPLPAKVVPLIAVPTTSGTGSQVTQAAV
ITDLEQHAKLTLFHPEFFPCEALIDPELMLTLPPRMTAMTGFDAFSHAFESFTGTRPSPFVDGMALEAMKLVIDHLPSVV
DNGADLHGRCQLAKADTLGGISLANGGAGAPHPLGEILGSSKTNLPHGLTLAVVYPAYAQLQWRKQPERFAQVAELFGAV
GSTEEKAQSLAAALVKFLERIGLESNLTQVGVTKEDVQKLEPAFCFDLPLTSGEEMKKVLQASLVE

Sequences:

>Translated_386_residues
MTQFQHYQPTKLTFGAGEIQKIGQLIAQYGTRCLVVSEPIFDAVKPAYDRIFTLLSEQGIEVTHFDGVVPNPPTTVVELG
RQVAIGNNCDVVLAIGGGSSIDTAKIISATINTESLDWAHWFATYDSPFGDVNPLPAKVVPLIAVPTTSGTGSQVTQAAV
ITDLEQHAKLTLFHPEFFPCEALIDPELMLTLPPRMTAMTGFDAFSHAFESFTGTRPSPFVDGMALEAMKLVIDHLPSVV
DNGADLHGRCQLAKADTLGGISLANGGAGAPHPLGEILGSSKTNLPHGLTLAVVYPAYAQLQWRKQPERFAQVAELFGAV
GSTEEKAQSLAAALVKFLERIGLESNLTQVGVTKEDVQKLEPAFCFDLPLTSGEEMKKVLQASLVE
>Mature_385_residues
TQFQHYQPTKLTFGAGEIQKIGQLIAQYGTRCLVVSEPIFDAVKPAYDRIFTLLSEQGIEVTHFDGVVPNPPTTVVELGR
QVAIGNNCDVVLAIGGGSSIDTAKIISATINTESLDWAHWFATYDSPFGDVNPLPAKVVPLIAVPTTSGTGSQVTQAAVI
TDLEQHAKLTLFHPEFFPCEALIDPELMLTLPPRMTAMTGFDAFSHAFESFTGTRPSPFVDGMALEAMKLVIDHLPSVVD
NGADLHGRCQLAKADTLGGISLANGGAGAPHPLGEILGSSKTNLPHGLTLAVVYPAYAQLQWRKQPERFAQVAELFGAVG
STEEKAQSLAAALVKFLERIGLESNLTQVGVTKEDVQKLEPAFCFDLPLTSGEEMKKVLQASLVE

Specific function: Essential for the utilization of choline as a precursor [H]

COG id: COG1454

COG function: function code C; Alcohol dehydrogenase, class IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI133922590, Length=389, Percent_Identity=28.5347043701799, Blast_Score=122, Evalue=5e-28,
Organism=Escherichia coli, GI48994951, Length=394, Percent_Identity=29.1878172588832, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1789163, Length=375, Percent_Identity=30.1333333333333, Blast_Score=141, Evalue=7e-35,
Organism=Escherichia coli, GI1787493, Length=387, Percent_Identity=29.1989664082687, Blast_Score=135, Evalue=7e-33,
Organism=Escherichia coli, GI87082107, Length=358, Percent_Identity=28.4916201117318, Blast_Score=134, Evalue=9e-33,
Organism=Escherichia coli, GI1789386, Length=377, Percent_Identity=27.0557029177719, Blast_Score=101, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17537053, Length=397, Percent_Identity=25.6926952141058, Blast_Score=117, Evalue=9e-27,
Organism=Saccharomyces cerevisiae, GI6321181, Length=360, Percent_Identity=30.5555555555556, Blast_Score=162, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24657991, Length=392, Percent_Identity=30.1020408163265, Blast_Score=129, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: =1.1.1.1 [H]

Molecular weight: Translated: 41369; Mature: 41238

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00913 ADH_IRON_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQFQHYQPTKLTFGAGEIQKIGQLIAQYGTRCLVVSEPIFDAVKPAYDRIFTLLSEQGI
CCCCCCCCCCEEEECCHHHHHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHHHHHHCCC
EVTHFDGVVPNPPTTVVELGRQVAIGNNCDVVLAIGGGSSIDTAKIISATINTESLDWAH
EEEEECCCCCCCCHHHHHCCCEEEECCCCCEEEEECCCCCCCHHHHEEEECCCCCCCHHH
WFATYDSPFGDVNPLPAKVVPLIAVPTTSGTGSQVTQAAVITDLEQHAKLTLFHPEFFPC
HHEECCCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCH
EALIDPELMLTLPPRMTAMTGFDAFSHAFESFTGTRPSPFVDGMALEAMKLVIDHLPSVV
HHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
DNGADLHGRCQLAKADTLGGISLANGGAGAPHPLGEILGSSKTNLPHGLTLAVVYPAYAQ
CCCCCCCCEEEEECCCCCCCEEECCCCCCCCCHHHHHHCCCCCCCCCCEEEEEECCHHHH
LQWRKQPERFAQVAELFGAVGSTEEKAQSLAAALVKFLERIGLESNLTQVGVTKEDVQKL
HHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHC
EPAFCFDLPLTSGEEMKKVLQASLVE
CCCEEEECCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
TQFQHYQPTKLTFGAGEIQKIGQLIAQYGTRCLVVSEPIFDAVKPAYDRIFTLLSEQGI
CCCCCCCCCEEEECCHHHHHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHHHHHHCCC
EVTHFDGVVPNPPTTVVELGRQVAIGNNCDVVLAIGGGSSIDTAKIISATINTESLDWAH
EEEEECCCCCCCCHHHHHCCCEEEECCCCCEEEEECCCCCCCHHHHEEEECCCCCCCHHH
WFATYDSPFGDVNPLPAKVVPLIAVPTTSGTGSQVTQAAVITDLEQHAKLTLFHPEFFPC
HHEECCCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCH
EALIDPELMLTLPPRMTAMTGFDAFSHAFESFTGTRPSPFVDGMALEAMKLVIDHLPSVV
HHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
DNGADLHGRCQLAKADTLGGISLANGGAGAPHPLGEILGSSKTNLPHGLTLAVVYPAYAQ
CCCCCCCCEEEEECCCCCCCEEECCCCCCCCCHHHHHHCCCCCCCCCCEEEEEECCHHHH
LQWRKQPERFAQVAELFGAVGSTEEKAQSLAAALVKFLERIGLESNLTQVGVTKEDVQKL
HHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHC
EPAFCFDLPLTSGEEMKKVLQASLVE
CCCEEEECCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8752328; 9384377 [H]