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The map label for this gene is recG [H]

Identifier: 218708221

GI number: 218708221

Start: 151261

End: 153351

Strand: Reverse

Name: recG [H]

Synonym: VS_0158

Alternate gene names: 218708221

Gene position: 153351-151261 (Counterclockwise)

Preceding gene: 218708222

Following gene: 218708218

Centisome position: 4.65

GC content: 49.21

Gene sequence:

>2091_bases
ATGAGCCTTGTTATGTCACAGCTTTTCTCTGCTATCCCTCTCAACTCTTTATCTGGAGTCGGCGCAAAAGTCGCAGAGAA
ACTGGAAAAGGTTGGGCTTAACAATGTACAAGACCTGCTATTTCACCTACCTCTGCGCTATGAAGATAGAACTCGTATCT
ATCCCATCGTAAAGCTGCATGCCGGGCTGTGGGCTGCTGTACAAGGGAAGGTGATGCATGTTGATACCATTTTCGGTAAA
CGTAAGATGCTTGCAGTAAAGATCAGCGATGGTAACGGCACCATCACCCTACGCTTTTTCAACTTCACCGCCGGTATGAA
GAATAACTTTGCCGAAGGCAAACAAGTGCATGCTTATGGTGAGATCAAGCGCGGCAATATGGGGCTTGAGATTGTCCATC
CGGATTACAAATTCTTTGCTCCTAGGCAACAACCAGACGTTGAAGCCAACCTGACTCCGGTGTATCCAACCACTGAAGGG
CTAAGACAAGTCACGCTACGTAACCTTACAGATCAAGCCTTGGAGTTAATCGACAAGGCAGCGGTTAATGAGCTTTTACC
ATCAGGCTTATACGACCACCAAATTACCCTCGCACAAGCTCTGCATACCATTCACAGGCCGCCCCCGGGCATTGACCTAG
AGCTGTTTGATGAAGGCAAGCACCCTGCTCAACTGCGACTAATTATGGAAGAGCTACTGGCTCAAAATCTGTCTATGTTG
TCTGTTCGTAGCAAAGGGCAGCAAGACAAGGCAATGCCTTTTCCTCCAGTAAACACTCTTAAAGATAAGCTACTGGCTCA
GCTGCCGTTTTCACCAACCAATGCCCAAGCACGAGTGACCAAAGAGATCGAAGCTGACTTGGAAAAGCCGCACCCTATGA
TGCGTTTAGTGCAAGGTGATGTGGGCTCTGGTAAAACCTTAGTCGCTGCACTCGCGGCGGTTCGCGCACTCGAACACGGT
CAGCAAGTAGCACTTATGGCACCCACTGAACTACTCGCAGAACAACACGCGATTAACTTTGCCAACTGGTTTGAGGCAAT
GGGTATTCAAGTTGGATGGCTAGCAGGTAAGCTCAAAGGCAAAGCTCGTGAAACTGAACTAACACGTATTGCCAGCGGCG
AAGCGCAAATGGTGGTGGGAACTCATGCGCTATTTCAAGAGCATGTCGAATTCAAAAACCTTGGCTTAGTGATCATCGAT
GAGCAGCACCGATTTGGTGTCCACCAGCGATTAGAGCTGCGTGAAAAAGGCGCGAAGCAAGGTTATTACCCTCACCAACT
GGTGATGACCGCGACCCCAATCCCACGAACGTTAGCAATGACGGCCTATGCCGATCTTGAAACTTCTATTATTGATGAGT
TACCACCGGGGCGAACCCCAATTCAAACCGTCGCGATTCCTGATACCAAGCGCGATGACATTGTTGAACGTGTGAAAAAT
GCGTGTCTCAATGAAGGCAAACAAGCTTACTGGGTGTGTACGTTGATTGATGAATCGGAAGTACTGGAAGCTCAAGCTGC
AGCGGATACGGCAGAAGAGCTGCAACGCAAACTGCCCGACGTAAAAATTGGTTTGGTTCATGGCAGAATGAAACCCGCAG
AGAAACAAGCGGTGATGCAGGAATTCAAAGAGAACAAACTGCACCTGTTAGTTGCTACAACCGTGATTGAAGTAGGTGTG
GATGTACCGAATTCGAGCTTAATGATCATCGAGAACCCAGAGCGTCTTGGTCTAGCGCAACTGCACCAACTGCGTGGTCG
TGTTGGCCGTGGTTCGGTCGCAAGCCATTGTGTACTGTTGTACCACTCCCCACTCTCTAAAACCGCTCAGAAACGCTTAG
GGGTACTTCGTGAAAGTAACGATGGCTTTGTGATAGCTCAGCGTGACTTAGAGATCCGTGGCCCCGGTGAGCTACTTGGC
ACTAAACAAACAGGCTTGGCTGACTTTAAGATTGCCGACTTAGTCCGTGACCAACGTTTGATACCTGAAGTGCAGCGTAT
CGCGCGTCATATCCACGATAGCTACCCAGACAATGCCAAGGCGATCATCAACCGCTGGCTGGGTGAGCGCGATGTGTATT
CTAAGGCGTAA

Upstream 100 bases:

>100_bases
TTTTTTGTGGCTACGATTCTAGAGTTCCCCTAGCTTCATGCTATTCATGCCTGTACAAAAATCCAGTAAAATGCTTGAAT
AGATTATCTCTCTTACGTTT

Downstream 100 bases:

>100_bases
TTCGCCCTCATATCTTGCTTAAAACGTAAAAAGGCTTCTACAAGGAAGCCTTTTTCGTGCGTATAGCCTAAGGTTTCACA
GGGAAACTGATCTGCGCCTT

Product: ATP-dependent DNA helicase RecG

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 696; Mature: 695

Protein sequence:

>696_residues
MSLVMSQLFSAIPLNSLSGVGAKVAEKLEKVGLNNVQDLLFHLPLRYEDRTRIYPIVKLHAGLWAAVQGKVMHVDTIFGK
RKMLAVKISDGNGTITLRFFNFTAGMKNNFAEGKQVHAYGEIKRGNMGLEIVHPDYKFFAPRQQPDVEANLTPVYPTTEG
LRQVTLRNLTDQALELIDKAAVNELLPSGLYDHQITLAQALHTIHRPPPGIDLELFDEGKHPAQLRLIMEELLAQNLSML
SVRSKGQQDKAMPFPPVNTLKDKLLAQLPFSPTNAQARVTKEIEADLEKPHPMMRLVQGDVGSGKTLVAALAAVRALEHG
QQVALMAPTELLAEQHAINFANWFEAMGIQVGWLAGKLKGKARETELTRIASGEAQMVVGTHALFQEHVEFKNLGLVIID
EQHRFGVHQRLELREKGAKQGYYPHQLVMTATPIPRTLAMTAYADLETSIIDELPPGRTPIQTVAIPDTKRDDIVERVKN
ACLNEGKQAYWVCTLIDESEVLEAQAAADTAEELQRKLPDVKIGLVHGRMKPAEKQAVMQEFKENKLHLLVATTVIEVGV
DVPNSSLMIIENPERLGLAQLHQLRGRVGRGSVASHCVLLYHSPLSKTAQKRLGVLRESNDGFVIAQRDLEIRGPGELLG
TKQTGLADFKIADLVRDQRLIPEVQRIARHIHDSYPDNAKAIINRWLGERDVYSKA

Sequences:

>Translated_696_residues
MSLVMSQLFSAIPLNSLSGVGAKVAEKLEKVGLNNVQDLLFHLPLRYEDRTRIYPIVKLHAGLWAAVQGKVMHVDTIFGK
RKMLAVKISDGNGTITLRFFNFTAGMKNNFAEGKQVHAYGEIKRGNMGLEIVHPDYKFFAPRQQPDVEANLTPVYPTTEG
LRQVTLRNLTDQALELIDKAAVNELLPSGLYDHQITLAQALHTIHRPPPGIDLELFDEGKHPAQLRLIMEELLAQNLSML
SVRSKGQQDKAMPFPPVNTLKDKLLAQLPFSPTNAQARVTKEIEADLEKPHPMMRLVQGDVGSGKTLVAALAAVRALEHG
QQVALMAPTELLAEQHAINFANWFEAMGIQVGWLAGKLKGKARETELTRIASGEAQMVVGTHALFQEHVEFKNLGLVIID
EQHRFGVHQRLELREKGAKQGYYPHQLVMTATPIPRTLAMTAYADLETSIIDELPPGRTPIQTVAIPDTKRDDIVERVKN
ACLNEGKQAYWVCTLIDESEVLEAQAAADTAEELQRKLPDVKIGLVHGRMKPAEKQAVMQEFKENKLHLLVATTVIEVGV
DVPNSSLMIIENPERLGLAQLHQLRGRVGRGSVASHCVLLYHSPLSKTAQKRLGVLRESNDGFVIAQRDLEIRGPGELLG
TKQTGLADFKIADLVRDQRLIPEVQRIARHIHDSYPDNAKAIINRWLGERDVYSKA
>Mature_695_residues
SLVMSQLFSAIPLNSLSGVGAKVAEKLEKVGLNNVQDLLFHLPLRYEDRTRIYPIVKLHAGLWAAVQGKVMHVDTIFGKR
KMLAVKISDGNGTITLRFFNFTAGMKNNFAEGKQVHAYGEIKRGNMGLEIVHPDYKFFAPRQQPDVEANLTPVYPTTEGL
RQVTLRNLTDQALELIDKAAVNELLPSGLYDHQITLAQALHTIHRPPPGIDLELFDEGKHPAQLRLIMEELLAQNLSMLS
VRSKGQQDKAMPFPPVNTLKDKLLAQLPFSPTNAQARVTKEIEADLEKPHPMMRLVQGDVGSGKTLVAALAAVRALEHGQ
QVALMAPTELLAEQHAINFANWFEAMGIQVGWLAGKLKGKARETELTRIASGEAQMVVGTHALFQEHVEFKNLGLVIIDE
QHRFGVHQRLELREKGAKQGYYPHQLVMTATPIPRTLAMTAYADLETSIIDELPPGRTPIQTVAIPDTKRDDIVERVKNA
CLNEGKQAYWVCTLIDESEVLEAQAAADTAEELQRKLPDVKIGLVHGRMKPAEKQAVMQEFKENKLHLLVATTVIEVGVD
VPNSSLMIIENPERLGLAQLHQLRGRVGRGSVASHCVLLYHSPLSKTAQKRLGVLRESNDGFVIAQRDLEIRGPGELLGT
KQTGLADFKIADLVRDQRLIPEVQRIARHIHDSYPDNAKAIINRWLGERDVYSKA

Specific function: Critical role in recombination and DNA repair. Help process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with a 3' to 5' polarity. RecG unwind branched dup

COG id: COG1200

COG function: function code LK; RecG-like helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367254, Length=690, Percent_Identity=67.9710144927536, Blast_Score=962, Evalue=0.0,
Organism=Escherichia coli, GI1787357, Length=386, Percent_Identity=39.3782383419689, Blast_Score=245, Evalue=6e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR004609
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C; PF01336 tRNA_anti [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 77357; Mature: 77225

Theoretical pI: Translated: 7.91; Mature: 7.91

Prosite motif: PS00435 PEROXIDASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLVMSQLFSAIPLNSLSGVGAKVAEKLEKVGLNNVQDLLFHLPLRYEDRTRIYPIVKLH
CHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCEEEEEEEEC
AGLWAAVQGKVMHVDTIFGKRKMLAVKISDGNGTITLRFFNFTAGMKNNFAEGKQVHAYG
CCHHHHHCCCEEEEEEHHCCCEEEEEEEECCCCEEEEEEEEEECCCCCCCCCCCEEEEEE
EIKRGNMGLEIVHPDYKFFAPRQQPDVEANLTPVYPTTEGLRQVTLRNLTDQALELIDKA
CEECCCCCEEEECCCHHEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AVNELLPSGLYDHQITLAQALHTIHRPPPGIDLELFDEGKHPAQLRLIMEELLAQNLSML
HHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCHHHH
SVRSKGQQDKAMPFPPVNTLKDKLLAQLPFSPTNAQARVTKEIEADLEKPHPMMRLVQGD
HHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHCCC
VGSGKTLVAALAAVRALEHGQQVALMAPTELLAEQHAINFANWFEAMGIQVGWLAGKLKG
CCCCHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCHHHHHHHHCCEEEEEEHHCCC
KARETELTRIASGEAQMVVGTHALFQEHVEFKNLGLVIIDEQHRFGVHQRLELREKGAKQ
CCHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHCCEEEEECCHHCCHHHHHHHHHCCCCC
GYYPHQLVMTATPIPRTLAMTAYADLETSIIDELPPGRTPIQTVAIPDTKRDDIVERVKN
CCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHH
ACLNEGKQAYWVCTLIDESEVLEAQAAADTAEELQRKLPDVKIGLVHGRMKPAEKQAVMQ
HHHCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHH
EFKENKLHLLVATTVIEVGVDVPNSSLMIIENPERLGLAQLHQLRGRVGRGSVASHCVLL
HHHCCCEEEEEEEEHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCHHHEEEEE
YHSPLSKTAQKRLGVLRESNDGFVIAQRDLEIRGPGELLGTKQTGLADFKIADLVRDQRL
EECCHHHHHHHHHHHEEECCCCEEEEECCEEECCCHHHCCCCCCCCCCHHHHHHHHHHHH
IPEVQRIARHIHDSYPDNAKAIINRWLGERDVYSKA
HHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SLVMSQLFSAIPLNSLSGVGAKVAEKLEKVGLNNVQDLLFHLPLRYEDRTRIYPIVKLH
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCEEEEEEEEC
AGLWAAVQGKVMHVDTIFGKRKMLAVKISDGNGTITLRFFNFTAGMKNNFAEGKQVHAYG
CCHHHHHCCCEEEEEEHHCCCEEEEEEEECCCCEEEEEEEEEECCCCCCCCCCCEEEEEE
EIKRGNMGLEIVHPDYKFFAPRQQPDVEANLTPVYPTTEGLRQVTLRNLTDQALELIDKA
CEECCCCCEEEECCCHHEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AVNELLPSGLYDHQITLAQALHTIHRPPPGIDLELFDEGKHPAQLRLIMEELLAQNLSML
HHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCHHHH
SVRSKGQQDKAMPFPPVNTLKDKLLAQLPFSPTNAQARVTKEIEADLEKPHPMMRLVQGD
HHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHCCC
VGSGKTLVAALAAVRALEHGQQVALMAPTELLAEQHAINFANWFEAMGIQVGWLAGKLKG
CCCCHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCHHHHHHHHCCEEEEEEHHCCC
KARETELTRIASGEAQMVVGTHALFQEHVEFKNLGLVIIDEQHRFGVHQRLELREKGAKQ
CCHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHCCEEEEECCHHCCHHHHHHHHHCCCCC
GYYPHQLVMTATPIPRTLAMTAYADLETSIIDELPPGRTPIQTVAIPDTKRDDIVERVKN
CCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHH
ACLNEGKQAYWVCTLIDESEVLEAQAAADTAEELQRKLPDVKIGLVHGRMKPAEKQAVMQ
HHHCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHH
EFKENKLHLLVATTVIEVGVDVPNSSLMIIENPERLGLAQLHQLRGRVGRGSVASHCVLL
HHHCCCEEEEEEEEHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCHHHEEEEE
YHSPLSKTAQKRLGVLRESNDGFVIAQRDLEIRGPGELLGTKQTGLADFKIADLVRDQRL
EECCHHHHHHHHHHHEEECCCCEEEEECCEEECCCHHHCCCCCCCCCCHHHHHHHHHHHH
IPEVQRIARHIHDSYPDNAKAIINRWLGERDVYSKA
HHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]