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The map label for this gene is pckA

Identifier: 218708195

GI number: 218708195

Start: 124790

End: 126415

Strand: Reverse

Name: pckA

Synonym: VS_0131

Alternate gene names: 218708195

Gene position: 126415-124790 (Counterclockwise)

Preceding gene: 218708196

Following gene: 218708194

Centisome position: 3.83

GC content: 46.8

Gene sequence:

>1626_bases
ATGACCGTTATGGAACATACTAAGGCTGCACAAATTGATCTAACTAAGCACGGACTGACTGGCGTTACTGAAGTTCTTCG
TAACCCTAGCTACGAGCAGTTATTCGTTGAAGAGACACTGCCAGGTTTGGAAGGCTACGAAAAAGGCGTAGTAACGGAAC
TAGGCTCTGTTGCGGTTGACACTGGTATCTTTACTGGCCGCTCACCAAAAGATAAGTACATTGTTAAAGATGACACCACG
CGCGATACCATGTGGTGGTCAGATCAAGGCAAAAACGACAACAAACCGATTACAACTGAAGTATGGGATGAGCTTAAAGA
GCTTGTGACAACTCAGCTATCTGGCAAGCGCCTGTTTGTCATTGACGGTTACTGTGGTGCTAACCCTGATACGCGCTTAA
GTGTACGTATTATCACTGAAGTAGCATGGCAAGCGCACTTCGTTAAGAACATGTTCATTCGTCCAACAGACGAAGAGCTA
GCAACGTTCGAACCTGATTTCGTGGTAATGAACGGTGCTAAAACAACTAACCCTAACTGGGAAAAGCAGGGTCTCAACTC
TGAAAACTTCGTTGCTTTCAACCTAACAGAACGCGTTCAAATCATCGGTGGTACTTGGTACGGCGGTGAGATGAAGAAAG
GCATGTTCGCAATGATGAACTACCTACTTCCTCTGCAAGGCATTGCTTCAATGCACTGTAGTGCTAACGTCGGTGAAGAA
GGCGATGTGGCGATTTTCTTCGGTCTATCAGGCACAGGTAAAACAACCCTTTCAACCGACCCTAAACGTGAGCTAATCGG
TGATGATGAGCACGGTTGGGATGACGATGGTATCTTCAATTTCGAAGGTGGTTGTTACGCCAAGACCATTCGCCTATCTA
AAGAAGCAGAACCTGAAATCTACAATGCAATCCGTCGCGATGCGCTACTAGAAAACGTAACGGTTCGTGGCGATGGTTCT
ATCGATTTTGATGACGGCTCAAAAACCGAGAACACTCGTGTTTCTTACCCGATTCACCATATCGACAACATCGTTAAGCC
AGTATCAAAAGCCGGTCACGCTCAAAAGGTTATCTTCCTGACTGCTGATGCATTTGGTGTGTTACCACCTGTTTCTAAAC
TGACTCCAGAGCAAACGAAGTACCACTTCCTATCTGGCTTCACTGCGAAACTAGCAGGTACAGAGCGTGGTATTACTGAA
CCAACACCAACGTTCTCTGCGGCATTTGGGGCGGCATTCCTAACTCTTCACCCAACTCAGTACGCAGAAGTACTTGTGAA
GCGTATGGAAGCAGCTGGCGCTGAAGCTTACCTTGTGAACACTGGTTGGAATGGCACTGGCAAACGTATCTCAATCCAAG
ATACTCGTGGCATCATCGACGCTATCCTAGATGGCTCAATCGACCAAGCTGAGACTAAGGTTATCCCTATGTTTAACCTA
GAAGTACCACTAGCGTTGCACGATGTTGACCCAGCGATTCTTGACCCACGCGATACATACACTGACCCTCTACAATGGGA
AAGCAAAGCGAAAGATCTAGCAGAGCGCTTCATCAACAACTTTGATAAGTACACAGACAACGCTGAAGGTAAGTCACTGG
TTGCGGCAGGCCCTCAACTGGACTAA

Upstream 100 bases:

>100_bases
TATATCCCTGTTTCCCCCGAGCCTCGTTGCTAGCATGGTGAGCAGATAACAATAAAAAATTAATACAAAATCCCTACAAA
ATATCCTACAAGGAGCACCT

Downstream 100 bases:

>100_bases
CACAACCATCTGCTCGGGCTTAGCCAAAAGCGAACGTCCAATTTAAAGTGATATTTTTGTAGCAAGCCCCTCTTTTGAGG
GGCTTTTTTGTTGCTGCTTG

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase

Number of amino acids: Translated: 541; Mature: 540

Protein sequence:

>541_residues
MTVMEHTKAAQIDLTKHGLTGVTEVLRNPSYEQLFVEETLPGLEGYEKGVVTELGSVAVDTGIFTGRSPKDKYIVKDDTT
RDTMWWSDQGKNDNKPITTEVWDELKELVTTQLSGKRLFVIDGYCGANPDTRLSVRIITEVAWQAHFVKNMFIRPTDEEL
ATFEPDFVVMNGAKTTNPNWEKQGLNSENFVAFNLTERVQIIGGTWYGGEMKKGMFAMMNYLLPLQGIASMHCSANVGEE
GDVAIFFGLSGTGKTTLSTDPKRELIGDDEHGWDDDGIFNFEGGCYAKTIRLSKEAEPEIYNAIRRDALLENVTVRGDGS
IDFDDGSKTENTRVSYPIHHIDNIVKPVSKAGHAQKVIFLTADAFGVLPPVSKLTPEQTKYHFLSGFTAKLAGTERGITE
PTPTFSAAFGAAFLTLHPTQYAEVLVKRMEAAGAEAYLVNTGWNGTGKRISIQDTRGIIDAILDGSIDQAETKVIPMFNL
EVPLALHDVDPAILDPRDTYTDPLQWESKAKDLAERFINNFDKYTDNAEGKSLVAAGPQLD

Sequences:

>Translated_541_residues
MTVMEHTKAAQIDLTKHGLTGVTEVLRNPSYEQLFVEETLPGLEGYEKGVVTELGSVAVDTGIFTGRSPKDKYIVKDDTT
RDTMWWSDQGKNDNKPITTEVWDELKELVTTQLSGKRLFVIDGYCGANPDTRLSVRIITEVAWQAHFVKNMFIRPTDEEL
ATFEPDFVVMNGAKTTNPNWEKQGLNSENFVAFNLTERVQIIGGTWYGGEMKKGMFAMMNYLLPLQGIASMHCSANVGEE
GDVAIFFGLSGTGKTTLSTDPKRELIGDDEHGWDDDGIFNFEGGCYAKTIRLSKEAEPEIYNAIRRDALLENVTVRGDGS
IDFDDGSKTENTRVSYPIHHIDNIVKPVSKAGHAQKVIFLTADAFGVLPPVSKLTPEQTKYHFLSGFTAKLAGTERGITE
PTPTFSAAFGAAFLTLHPTQYAEVLVKRMEAAGAEAYLVNTGWNGTGKRISIQDTRGIIDAILDGSIDQAETKVIPMFNL
EVPLALHDVDPAILDPRDTYTDPLQWESKAKDLAERFINNFDKYTDNAEGKSLVAAGPQLD
>Mature_540_residues
TVMEHTKAAQIDLTKHGLTGVTEVLRNPSYEQLFVEETLPGLEGYEKGVVTELGSVAVDTGIFTGRSPKDKYIVKDDTTR
DTMWWSDQGKNDNKPITTEVWDELKELVTTQLSGKRLFVIDGYCGANPDTRLSVRIITEVAWQAHFVKNMFIRPTDEELA
TFEPDFVVMNGAKTTNPNWEKQGLNSENFVAFNLTERVQIIGGTWYGGEMKKGMFAMMNYLLPLQGIASMHCSANVGEEG
DVAIFFGLSGTGKTTLSTDPKRELIGDDEHGWDDDGIFNFEGGCYAKTIRLSKEAEPEIYNAIRRDALLENVTVRGDGSI
DFDDGSKTENTRVSYPIHHIDNIVKPVSKAGHAQKVIFLTADAFGVLPPVSKLTPEQTKYHFLSGFTAKLAGTERGITEP
TPTFSAAFGAAFLTLHPTQYAEVLVKRMEAAGAEAYLVNTGWNGTGKRISIQDTRGIIDAILDGSIDQAETKVIPMFNLE
VPLALHDVDPAILDPRDTYTDPLQWESKAKDLAERFINNFDKYTDNAEGKSLVAAGPQLD

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family

Homologues:

Organism=Escherichia coli, GI1789807, Length=530, Percent_Identity=80.377358490566, Blast_Score=904, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6322950, Length=534, Percent_Identity=45.5056179775281, Blast_Score=454, Evalue=1e-128,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): PCKA_VIBSL (B7VHF2)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002415816.1
- ProteinModelPortal:   B7VHF2
- SMR:   B7VHF2
- GeneID:   7159804
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_0131
- HOGENOM:   HBG293387
- OMA:   MFIRPTE
- ProtClustDB:   PRK09344
- GO:   GO:0005737
- GO:   GO:0006094
- HAMAP:   MF_00453
- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- InterPro:   IPR015994
- Gene3D:   G3DSA:3.90.228.20
- Gene3D:   G3DSA:3.40.449.10
- PIRSF:   PIRSF006294
- TIGRFAMs:   TIGR00224

Pfam domain/function: PF01293 PEPCK_ATP; SSF68923 PEP_carboxykinase_N

EC number: =4.1.1.49

Molecular weight: Translated: 59756; Mature: 59625

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS00532 PEPCK_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVMEHTKAAQIDLTKHGLTGVTEVLRNPSYEQLFVEETLPGLEGYEKGVVTELGSVAVD
CCCCCCCCCEEEEEHHCCHHHHHHHHHCCCHHEEEHHHHCCCCCCHHCCHHHHHHHHHEE
TGIFTGRSPKDKYIVKDDTTRDTMWWSDQGKNDNKPITTEVWDELKELVTTQLSGKRLFV
CCEECCCCCCCCEEECCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEE
IDGYCGANPDTRLSVRIITEVAWQAHFVKNMFIRPTDEELATFEPDFVVMNGAKTTNPNW
EECCCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCHHHHCCCCCEEEECCCCCCCCCH
EKQGLNSENFVAFNLTERVQIIGGTWYGGEMKKGMFAMMNYLLPLQGIASMHCSANVGEE
HHCCCCCCCEEEEEECCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GDVAIFFGLSGTGKTTLSTDPKRELIGDDEHGWDDDGIFNFEGGCYAKTIRLSKEAEPEI
CCEEEEEEECCCCCCCCCCCCCHHHCCCCCCCCCCCCEEECCCCCEEEEEEECCCCCHHH
YNAIRRDALLENVTVRGDGSIDFDDGSKTENTRVSYPIHHIDNIVKPVSKAGHAQKVIFL
HHHHHHHHHHHCEEEECCCCEECCCCCCCCCCEEECCHHHHHHHHHHHHHCCCCEEEEEE
TADAFGVLPPVSKLTPEQTKYHFLSGFTAKLAGTERGITEPTPTFSAAFGAAFLTLHPTQ
EECCCCCCCCHHHCCCCHHHHHHHCCCCCEECCCCCCCCCCCCCHHHHHHEEEEEECCHH
YAEVLVKRMEAAGAEAYLVNTGWNGTGKRISIQDTRGIIDAILDGSIDQAETKVIPMFNL
HHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEHHHHHHHHHHHCCCCCCCCCEEEEEEEC
EVPLALHDVDPAILDPRDTYTDPLQWESKAKDLAERFINNFDKYTDNAEGKSLVAAGPQL
CCCEEEECCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCC
D
C
>Mature Secondary Structure 
TVMEHTKAAQIDLTKHGLTGVTEVLRNPSYEQLFVEETLPGLEGYEKGVVTELGSVAVD
CCCCCCCCEEEEEHHCCHHHHHHHHHCCCHHEEEHHHHCCCCCCHHCCHHHHHHHHHEE
TGIFTGRSPKDKYIVKDDTTRDTMWWSDQGKNDNKPITTEVWDELKELVTTQLSGKRLFV
CCEECCCCCCCCEEECCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEE
IDGYCGANPDTRLSVRIITEVAWQAHFVKNMFIRPTDEELATFEPDFVVMNGAKTTNPNW
EECCCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCHHHHCCCCCEEEECCCCCCCCCH
EKQGLNSENFVAFNLTERVQIIGGTWYGGEMKKGMFAMMNYLLPLQGIASMHCSANVGEE
HHCCCCCCCEEEEEECCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GDVAIFFGLSGTGKTTLSTDPKRELIGDDEHGWDDDGIFNFEGGCYAKTIRLSKEAEPEI
CCEEEEEEECCCCCCCCCCCCCHHHCCCCCCCCCCCCEEECCCCCEEEEEEECCCCCHHH
YNAIRRDALLENVTVRGDGSIDFDDGSKTENTRVSYPIHHIDNIVKPVSKAGHAQKVIFL
HHHHHHHHHHHCEEEECCCCEECCCCCCCCCCEEECCHHHHHHHHHHHHHCCCCEEEEEE
TADAFGVLPPVSKLTPEQTKYHFLSGFTAKLAGTERGITEPTPTFSAAFGAAFLTLHPTQ
EECCCCCCCCHHHCCCCHHHHHHHCCCCCEECCCCCCCCCCCCCHHHHHHEEEEEECCHH
YAEVLVKRMEAAGAEAYLVNTGWNGTGKRISIQDTRGIIDAILDGSIDQAETKVIPMFNL
HHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEHHHHHHHHHHHCCCCCCCCCEEEEEEEC
EVPLALHDVDPAILDPRDTYTDPLQWESKAKDLAERFINNFDKYTDNAEGKSLVAAGPQL
CCCEEEECCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCC
D
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA