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The map label for this gene is hemB [H]

Identifier: 218708171

GI number: 218708171

Start: 96544

End: 97587

Strand: Direct

Name: hemB [H]

Synonym: VS_0105

Alternate gene names: 218708171

Gene position: 96544-97587 (Clockwise)

Preceding gene: 218708169

Following gene: 218708172

Centisome position: 2.93

GC content: 47.7

Gene sequence:

>1044_bases
GTGTCTGTTTCAATTCAAGGTCAATTCCCAGGTCGCCGTATGCGCCGTATGCGTAAACACGACTTTAGCCGTCGCCTAAT
GGCAGAAAATCAATTGTCTGTGGATGACCTCATCTACCCAATGTTTATCCTTATGGGTAAAGACCGCCGCGAGCCTGTCG
AGTCAATGCCGGGTGTTGAACGTTTGTCGATCGATCTGATGCTTGAAGAAGCGGATTACCTGTCAAAATTGGGTGTTCCG
GCGATTGCTCTGTTTCCGGTCGTGAACCAAGATGCTAAAAGCTTATGCGCGGCTGAAGCTCATAATTCAGAAGGTTTAGT
GCAGCGTGCGGTACGTTCATTAAAAGAGCACGTGCCAAATATGGGTGTGATTACTGATGTTGCACTGGACCCATTTACTA
CTCACGGCCAGGACGGCATCATCGATGAAGATGGTTACGTGATGAATGATGAAACGACTGAAGTGTTAATTCAGCAGGCG
TTATCGCATGCTGAAGCCGGTGCTGATGTGGTTGCTCCGTCGGATATGATGGATGGTCGTATTGGTAAGATCCGTGAAGC
GTTAGAGGAAGCGGCCTTTATTGCCTCTCAGATTATGGCGTACTCTGCGAAATACCCCTTGTGTTATTACGGCCCATTCC
GTGATGCAGTCGGCAGTGCTTCGAACCTGAAAGGTGGTAATAAGAAGAACTACCAGATGGATCCTGCCAACAGCGATGAA
GCGATTCACGAAGTGGCAATGGATCTTAATGAAGGTGCGGATATGGTGATGGTTAAGCCAGGCATGCCTTATTTAGACAT
CGTGCGTCGTGTGAAACATGAACTTCAAGCACCAACGTATGCTTATCAAGTGTCTGGTGAGTATGCGATGCACAAAGCAG
CAATTCAAAACGGTTGGCTGAAAGAACGCGACACCGTTATGGAATCACTGCTGTGCTTTAAGCGTGCTGGTGCTGATGGT
ATCCTGACGTACTTCGCTAAAGATGTTGCAGAGTGGCTTGCTGAAGACAATGCACAAGCAGCCAAGCACTTAAAAGAAAA
GTAA

Upstream 100 bases:

>100_bases
TACTGCTAGCGTCCTGAGCCATCAGTGATATGCTTTTGCCTGTATTTAGCACTTTTCATATCTAAGCATTTCGGTGCTTA
GACTAGGCAAGGAGAATCTA

Downstream 100 bases:

>100_bases
CAACTCAACGTTAACTTAAGTGATATTCAGTAAAAGGGTTGGCCATTGTGTCAGCCCTTTCGCTTATGAGGATGAAAGAA
GATGTCAGTCATAGTACGTG

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 347; Mature: 346

Protein sequence:

>347_residues
MSVSIQGQFPGRRMRRMRKHDFSRRLMAENQLSVDDLIYPMFILMGKDRREPVESMPGVERLSIDLMLEEADYLSKLGVP
AIALFPVVNQDAKSLCAAEAHNSEGLVQRAVRSLKEHVPNMGVITDVALDPFTTHGQDGIIDEDGYVMNDETTEVLIQQA
LSHAEAGADVVAPSDMMDGRIGKIREALEEAAFIASQIMAYSAKYPLCYYGPFRDAVGSASNLKGGNKKNYQMDPANSDE
AIHEVAMDLNEGADMVMVKPGMPYLDIVRRVKHELQAPTYAYQVSGEYAMHKAAIQNGWLKERDTVMESLLCFKRAGADG
ILTYFAKDVAEWLAEDNAQAAKHLKEK

Sequences:

>Translated_347_residues
MSVSIQGQFPGRRMRRMRKHDFSRRLMAENQLSVDDLIYPMFILMGKDRREPVESMPGVERLSIDLMLEEADYLSKLGVP
AIALFPVVNQDAKSLCAAEAHNSEGLVQRAVRSLKEHVPNMGVITDVALDPFTTHGQDGIIDEDGYVMNDETTEVLIQQA
LSHAEAGADVVAPSDMMDGRIGKIREALEEAAFIASQIMAYSAKYPLCYYGPFRDAVGSASNLKGGNKKNYQMDPANSDE
AIHEVAMDLNEGADMVMVKPGMPYLDIVRRVKHELQAPTYAYQVSGEYAMHKAAIQNGWLKERDTVMESLLCFKRAGADG
ILTYFAKDVAEWLAEDNAQAAKHLKEK
>Mature_346_residues
SVSIQGQFPGRRMRRMRKHDFSRRLMAENQLSVDDLIYPMFILMGKDRREPVESMPGVERLSIDLMLEEADYLSKLGVPA
IALFPVVNQDAKSLCAAEAHNSEGLVQRAVRSLKEHVPNMGVITDVALDPFTTHGQDGIIDEDGYVMNDETTEVLIQQAL
SHAEAGADVVAPSDMMDGRIGKIREALEEAAFIASQIMAYSAKYPLCYYGPFRDAVGSASNLKGGNKKNYQMDPANSDEA
IHEVAMDLNEGADMVMVKPGMPYLDIVRRVKHELQAPTYAYQVSGEYAMHKAAIQNGWLKERDTVMESLLCFKRAGADGI
LTYFAKDVAEWLAEDNAQAAKHLKEK

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=311, Percent_Identity=39.871382636656, Blast_Score=226, Evalue=2e-59,
Organism=Escherichia coli, GI87081728, Length=320, Percent_Identity=43.75, Blast_Score=236, Evalue=2e-63,
Organism=Saccharomyces cerevisiae, GI6321398, Length=325, Percent_Identity=36, Blast_Score=193, Evalue=3e-50,
Organism=Drosophila melanogaster, GI21358291, Length=316, Percent_Identity=37.9746835443038, Blast_Score=219, Evalue=3e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 38566; Mature: 38434

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
5.8 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVSIQGQFPGRRMRRMRKHDFSRRLMAENQLSVDDLIYPMFILMGKDRREPVESMPGVE
CCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHCCCCH
RLSIDLMLEEADYLSKLGVPAIALFPVVNQDAKSLCAAEAHNSEGLVQRAVRSLKEHVPN
HEEHHHHHHHHHHHHHCCCCCEEHHHHHCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
MGVITDVALDPFTTHGQDGIIDEDGYVMNDETTEVLIQQALSHAEAGADVVAPSDMMDGR
CCEEEHHCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCCCCCHHHHCCH
IGKIREALEEAAFIASQIMAYSAKYPLCYYGPFRDAVGSASNLKGGNKKNYQMDPANSDE
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHCCCCCCCCCCCCCCCCCCCCCHH
AIHEVAMDLNEGADMVMVKPGMPYLDIVRRVKHELQAPTYAYQVSGEYAMHKAAIQNGWL
HHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHCCCC
KERDTVMESLLCFKRAGADGILTYFAKDVAEWLAEDNAQAAKHLKEK
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCC
>Mature Secondary Structure 
SVSIQGQFPGRRMRRMRKHDFSRRLMAENQLSVDDLIYPMFILMGKDRREPVESMPGVE
CEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHCCCCH
RLSIDLMLEEADYLSKLGVPAIALFPVVNQDAKSLCAAEAHNSEGLVQRAVRSLKEHVPN
HEEHHHHHHHHHHHHHCCCCCEEHHHHHCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
MGVITDVALDPFTTHGQDGIIDEDGYVMNDETTEVLIQQALSHAEAGADVVAPSDMMDGR
CCEEEHHCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCCCCCHHHHCCH
IGKIREALEEAAFIASQIMAYSAKYPLCYYGPFRDAVGSASNLKGGNKKNYQMDPANSDE
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHCCCCCCCCCCCCCCCCCCCCCHH
AIHEVAMDLNEGADMVMVKPGMPYLDIVRRVKHELQAPTYAYQVSGEYAMHKAAIQNGWL
HHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHCCCC
KERDTVMESLLCFKRAGADGILTYFAKDVAEWLAEDNAQAAKHLKEK
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9529530; 10984043; 10356331; 12079382 [H]