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The map label for this gene is dppA [H]

Identifier: 218708141

GI number: 218708141

Start: 64524

End: 66077

Strand: Direct

Name: dppA [H]

Synonym: VS_0075

Alternate gene names: 218708141

Gene position: 64524-66077 (Clockwise)

Preceding gene: 218708140

Following gene: 218708142

Centisome position: 1.96

GC content: 45.5

Gene sequence:

>1554_bases
ATGAAAACCATGAAAAGCAAATTAGCAGTAGCTTTAATGGCAGCTGGCCTAAGCTTTAGTGCAGCAGCAGCAGATATTAC
CGTTGGCTACGCAGCTGACCCGGTATCACTTGACCCGCACGAGCAGCTGTCTGGTGGCACACTGCAAATGTCTCACATGG
TGTTTGACCCTCTAGTACGTTTCACTCAAGAGATGGATTTTGAAGGCCGCCTAGCATCTAGCTGGGAGCGTGTTGATGAA
ACGACTTTCCGCTTTAACTTACGTAAAGGCGTGAAATTCCACTCTGGTAACGAACTGACTGCTGATGATGTTGTGTGGAC
TTTTGAACGTCTACAAGCTTCTCCAGACTTTAAGTCTATCTTCACGCCATACGAAAAAATGGTAAAAGTGGATGACTACA
CAGTTGAGCTAGTATCAAAAGCGGCTTACCCACTAGTACTACAAACAGCAACCTACATCTTCCCAATGGACAGCAAGTTC
TACTCTGGTCAAACAGCGGAAGGTAATGACAAATCTGAGCTAGTTAAGCACGGTAACTCGTTTGCTTCGACTAACGTTTC
TGGTACTGGTCCATTTATCGTAACGCAGCGTGAGCAAGGCGTTAAAGTAACGTTCGAGCGTTTCAACGATTACTGGGACA
CAGAAACTGAAGGTAACGTAGACAAGCTAACACTTGTGCCAATCAAAGAAGATGCAACGCGTGTAGCAGCACTTCTTTCT
GGCGACGTTGATATGATTCACCCTGTTGCGCCAAACGATCACAAGCGTGTTAAAAAAGCTAAGAACATCGATCTAGTAAC
GCTACCTGGTACTCGTATCATTACGTTCCAAATGAACCAAAACAGCAACGAAGCACTGAAAGATGTGCGCGTTCGTCAAG
CAATCGTTCATGCGATTAACAATGAAGGTATTGTTAAGAAAATCATGAAAGGTTTCGCGACAGCTGCTGGTCAGCAGAGC
CCAACAGGCTACGCGGGTCACAACGAAGATCTAGTTCCTCGTTACGATCTGAAAAAAGCGAAAGAGCTGATGAAAGAAGC
GGGCTACGAAGACGGCTTTACACTAACTATGGCAGCGCCTAACAACCGTTACGTGAACGATGCGAAAGTTGCGCAAGCGT
CTGCGGCAATGCTGTCTAAGATTGGTATCAAAGTTGATCTTAAGACAATGCCTAAAGCACAGTACTGGCCAGAATTTGAT
CTATGTTCAGCAGACATGATGATGATCGGCTGGCACTCAGATACTGAAGATTCAGCTAACTTCAACGAGTTCCTAACAAT
GACTCGTAACGAAGAGACTGGCCGTGGTCAATACAACTGTTCTGGCTACTCAAACCCAGAAATGGATGCGATTGTAGAAG
CTTCTAATACTGAAACTGACCCAGTTAAGCGTTCTAAGATGCTGAAAGGAGTTGAAGCAACACTTTACAACGATGCAGTC
TTCGTTCCTCTACACTGGCAGAGTGAAGCGTGGGGCGCGAAATCTAACGTAGGTGCAGCAGACGTAGTAAACCCAATGGT
TATGCCTTACTTCGGCGACCTAGTTGTAAAATAA

Upstream 100 bases:

>100_bases
GTATAAAAATCAGCAGGCTGGAGTTTAGCTTGCTAAAAACAATAAACAGACGCAAATACAACAACTAGGGATCTGGATTC
CCGCATGAAGGAGTTATGCA

Downstream 100 bases:

>100_bases
TCTAGCTTGCTAGAATCGAGAGCCTGAAGCTTTCAGGCTCTCAATATTATTAAGATATAGATTTAAGTTTCGGTATTTGG
TCTTCCTTCAGTCTGGCTAA

Product: periplasmic dipeptide transport protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: Dipeptide-binding protein; DBP [H]

Number of amino acids: Translated: 517; Mature: 517

Protein sequence:

>517_residues
MKTMKSKLAVALMAAGLSFSAAAADITVGYAADPVSLDPHEQLSGGTLQMSHMVFDPLVRFTQEMDFEGRLASSWERVDE
TTFRFNLRKGVKFHSGNELTADDVVWTFERLQASPDFKSIFTPYEKMVKVDDYTVELVSKAAYPLVLQTATYIFPMDSKF
YSGQTAEGNDKSELVKHGNSFASTNVSGTGPFIVTQREQGVKVTFERFNDYWDTETEGNVDKLTLVPIKEDATRVAALLS
GDVDMIHPVAPNDHKRVKKAKNIDLVTLPGTRIITFQMNQNSNEALKDVRVRQAIVHAINNEGIVKKIMKGFATAAGQQS
PTGYAGHNEDLVPRYDLKKAKELMKEAGYEDGFTLTMAAPNNRYVNDAKVAQASAAMLSKIGIKVDLKTMPKAQYWPEFD
LCSADMMMIGWHSDTEDSANFNEFLTMTRNEETGRGQYNCSGYSNPEMDAIVEASNTETDPVKRSKMLKGVEATLYNDAV
FVPLHWQSEAWGAKSNVGAADVVNPMVMPYFGDLVVK

Sequences:

>Translated_517_residues
MKTMKSKLAVALMAAGLSFSAAAADITVGYAADPVSLDPHEQLSGGTLQMSHMVFDPLVRFTQEMDFEGRLASSWERVDE
TTFRFNLRKGVKFHSGNELTADDVVWTFERLQASPDFKSIFTPYEKMVKVDDYTVELVSKAAYPLVLQTATYIFPMDSKF
YSGQTAEGNDKSELVKHGNSFASTNVSGTGPFIVTQREQGVKVTFERFNDYWDTETEGNVDKLTLVPIKEDATRVAALLS
GDVDMIHPVAPNDHKRVKKAKNIDLVTLPGTRIITFQMNQNSNEALKDVRVRQAIVHAINNEGIVKKIMKGFATAAGQQS
PTGYAGHNEDLVPRYDLKKAKELMKEAGYEDGFTLTMAAPNNRYVNDAKVAQASAAMLSKIGIKVDLKTMPKAQYWPEFD
LCSADMMMIGWHSDTEDSANFNEFLTMTRNEETGRGQYNCSGYSNPEMDAIVEASNTETDPVKRSKMLKGVEATLYNDAV
FVPLHWQSEAWGAKSNVGAADVVNPMVMPYFGDLVVK
>Mature_517_residues
MKTMKSKLAVALMAAGLSFSAAAADITVGYAADPVSLDPHEQLSGGTLQMSHMVFDPLVRFTQEMDFEGRLASSWERVDE
TTFRFNLRKGVKFHSGNELTADDVVWTFERLQASPDFKSIFTPYEKMVKVDDYTVELVSKAAYPLVLQTATYIFPMDSKF
YSGQTAEGNDKSELVKHGNSFASTNVSGTGPFIVTQREQGVKVTFERFNDYWDTETEGNVDKLTLVPIKEDATRVAALLS
GDVDMIHPVAPNDHKRVKKAKNIDLVTLPGTRIITFQMNQNSNEALKDVRVRQAIVHAINNEGIVKKIMKGFATAAGQQS
PTGYAGHNEDLVPRYDLKKAKELMKEAGYEDGFTLTMAAPNNRYVNDAKVAQASAAMLSKIGIKVDLKTMPKAQYWPEFD
LCSADMMMIGWHSDTEDSANFNEFLTMTRNEETGRGQYNCSGYSNPEMDAIVEASNTETDPVKRSKMLKGVEATLYNDAV
FVPLHWQSEAWGAKSNVGAADVVNPMVMPYFGDLVVK

Specific function: Dipeptide-binding protein of a transport system that can be subject to osmotic shock. DppA is also required for peptide chemotaxis [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=503, Percent_Identity=28.0318091451292, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1787052, Length=488, Percent_Identity=25.2049180327869, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI1787762, Length=529, Percent_Identity=24.1965973534972, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI87081878, Length=521, Percent_Identity=22.8406909788868, Blast_Score=108, Evalue=8e-25,
Organism=Escherichia coli, GI1787495, Length=572, Percent_Identity=23.4265734265734, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787551, Length=443, Percent_Identity=24.8306997742664, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1789397, Length=521, Percent_Identity=22.8406909788868, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789887, Length=536, Percent_Identity=22.9477611940299, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 57322; Mature: 57322

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTMKSKLAVALMAAGLSFSAAAADITVGYAADPVSLDPHEQLSGGTLQMSHMVFDPLVR
CCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCHHHCCCCEEEEHHHHHHHHHH
FTQEMDFEGRLASSWERVDETTFRFNLRKGVKFHSGNELTADDVVWTFERLQASPDFKSI
HHHHCCCCCCHHHHHHHHCCCEEEEECCCCCEECCCCCCCHHHHHHHHHHHCCCCCHHHH
FTPYEKMVKVDDYTVELVSKAAYPLVLQTATYIFPMDSKFYSGQTAEGNDKSELVKHGNS
HCCHHHHEECCCHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCCCCCCCHHHHHHCCCC
FASTNVSGTGPFIVTQREQGVKVTFERFNDYWDTETEGNVDKLTLVPIKEDATRVAALLS
CCCCCCCCCCCEEEEECCCCCEEEEEHHCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHC
GDVDMIHPVAPNDHKRVKKAKNIDLVTLPGTRIITFQMNQNSNEALKDVRVRQAIVHAIN
CCCCEECCCCCCHHHHHHHHCCCEEEEECCCEEEEEEECCCCHHHHHHHHHHHHHHHHHC
NEGIVKKIMKGFATAAGQQSPTGYAGHNEDLVPRYDLKKAKELMKEAGYEDGFTLTMAAP
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECC
NNRYVNDAKVAQASAAMLSKIGIKVDLKTMPKAQYWPEFDLCSADMMMIGWHSDTEDSAN
CCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCC
FNEFLTMTRNEETGRGQYNCSGYSNPEMDAIVEASNTETDPVKRSKMLKGVEATLYNDAV
HHHHEEEECCCCCCCCCEECCCCCCCCCCEEEECCCCCCCHHHHHHHHHCHHHEEECCEE
FVPLHWQSEAWGAKSNVGAADVVNPMVMPYFGDLVVK
EEEEEECCCCCCCCCCCCHHHHHCCHHHCCCCCEECC
>Mature Secondary Structure
MKTMKSKLAVALMAAGLSFSAAAADITVGYAADPVSLDPHEQLSGGTLQMSHMVFDPLVR
CCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCHHHCCCCEEEEHHHHHHHHHH
FTQEMDFEGRLASSWERVDETTFRFNLRKGVKFHSGNELTADDVVWTFERLQASPDFKSI
HHHHCCCCCCHHHHHHHHCCCEEEEECCCCCEECCCCCCCHHHHHHHHHHHCCCCCHHHH
FTPYEKMVKVDDYTVELVSKAAYPLVLQTATYIFPMDSKFYSGQTAEGNDKSELVKHGNS
HCCHHHHEECCCHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCCCCCCCHHHHHHCCCC
FASTNVSGTGPFIVTQREQGVKVTFERFNDYWDTETEGNVDKLTLVPIKEDATRVAALLS
CCCCCCCCCCCEEEEECCCCCEEEEEHHCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHC
GDVDMIHPVAPNDHKRVKKAKNIDLVTLPGTRIITFQMNQNSNEALKDVRVRQAIVHAIN
CCCCEECCCCCCHHHHHHHHCCCEEEEECCCEEEEEEECCCCHHHHHHHHHHHHHHHHHC
NEGIVKKIMKGFATAAGQQSPTGYAGHNEDLVPRYDLKKAKELMKEAGYEDGFTLTMAAP
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECC
NNRYVNDAKVAQASAAMLSKIGIKVDLKTMPKAQYWPEFDLCSADMMMIGWHSDTEDSAN
CCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCC
FNEFLTMTRNEETGRGQYNCSGYSNPEMDAIVEASNTETDPVKRSKMLKGVEATLYNDAV
HHHHEEEECCCCCCCCCEECCCCCCCCCCEEEECCCCCCCHHHHHHHHHCHHHEEECCEE
FVPLHWQSEAWGAKSNVGAADVVNPMVMPYFGDLVVK
EEEEEECCCCCCCCCCCCHHHHHCCHHHCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1702779; 1956284; 7536291; 8041620; 9278503; 9298646; 9600841; 8563629; 8527431 [H]