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The map label for this gene is glyS

Identifier: 218708120

GI number: 218708120

Start: 30042

End: 32108

Strand: Reverse

Name: glyS

Synonym: VS_0032

Alternate gene names: 218708120

Gene position: 32108-30042 (Counterclockwise)

Preceding gene: 218708121

Following gene: 218708119

Centisome position: 0.97

GC content: 47.27

Gene sequence:

>2067_bases
ATGGCGAAGAATTTTCTAATTGAACTAGGTACGGAAGAGCTTCCACCAACAGCACTTCGTTCTCTAGCAGAAGCCTTTGC
TTCTAACTTTGAAGCGGGTCTTAAAACGGCTGAGCTTTCTCACGAAGGCATCAAGTGGTACGCAGCACCTCGTCGTCTAG
CACTTAAAGTGACTGCACTGGCTGAAGGCCAAGCAGACAAAGTCGTTGAGAAGCGCGGTCCTGCAATTTCTGTCGCATTC
GATGCGGAAGGCAACGCGACTAAAGCTGCACAAGGTTGGGCTCGTGGTAACGGTATTACTGTTGAGCAAGCTGACCGCCT
GAAAACAGACAAAGGCGAGTGGCTTCTTTTCAAACAAGAAGTGGCTGGCAAACCTGTTCAAGAATTGGTGATGGACATCG
CTGCGAAAGCTCTCGCTGGCCTACCAATTCCTAAAGCAATGCGCTGGGGTAACTCAGACATTCAATTTATCCGTCCAGTG
AAAACACTGACAGTACTACTAGGTGATGAGCTTGTTGAAGGCAAAATCCTAGGCGTAGCTTCTGCTCGTACTATCCGTGG
CCACCGTTTCATGGGCGAACAAGAGTTCACAATCGACTCTGCTGACCAATACCCTGCGATCTTAGAAGAGCGCGGTAAAG
TAATGGCAGATTACGATGCGCGTAAAGCGATCATTCTTGCTGATGCTAAAAAAGCAGCTGACGCAGTTGGTGGTATTGCA
GACCTAGAAGATGACCTTGTTGAAGAAGTTACTTCTTTGGTTGAATGGCCAGTGGTGCTTACTGCTAAGTTTGAGCAAGT
GTTCCTAAAAGTGCCTTCTGAAGCTTTGGTTTACACCATGAAAGGCGACCAGAAGTACTTCCCAGTGTATGACGCTGATA
AAAATCTTCTGCCAAACTTCATCTTCGTTTCGAACATCGAGTCTAAAGAGCCTCGTCACGTAATCGAAGGTAACGAGAAG
GTTGTACGTCCACGTCTTGCTGATGCTGAATTCTTCTTCAACACAGACCGCAAACGTCCGCTGATCGACCGTCTTGCTGA
ACTAGACCAAGCTATCTTCCAGAAGCAATTAGGTACTATCAAAGACAAAACCGATCGCATCACAGAGCTTGCTGGCTACA
TTGCTGAGCAAATCGATGCTGACGTTGAAAAGTCTAAGCGTGCTGGCCTACTGGCTAAGTGTGACCTAATGACATCTATG
GTATTCGAGTTTACGGATACTCAAGGTGTGATGGGCATGCACTACGCGACTCACGATGGTGAAGACGAGCAAGTAGCACT
AGCACTTTACGAGCAGTACATGCCTCGTTTCGCAGGTGACACACTACCAAGCACTGGCATCTCTTCTGCTGTAGCAATGG
CAGACAAGCTAGACACTATCGTGGGTATCTTCGGTATTGGCCAAGCACCAAAAGGTTCTGACCCATTCGCTCTACGTCGT
GCATCACTAGGTGTGCTACGTATCATCGTTGAAAACGGCTACAACCTAGACCTAACTGATCTAATCGGTAAAGCGAAAGA
GCTACTAGGCGACAAGCTAACCAACGAAAACGTAGAAGCTGACGTTATCGACTTCATGCTAGGTCGTTTCCGCGCATGGT
ACCAAGATGCTGGTTTCAGTGTTGATATCATCCAAGCGGTACTAGCACGTCGTCCAACCAAGCCAGCTGACTTTGACCAA
CGTGTTAAAGCGGTTTCTCACTTCCGTGAACTGGAAGCAGCAGAAGCACTAGCAGCAGCGAACAAGCGTGTAGGTAACAT
CCTTGCTAAATTCGATGGCGAGCTACCTGCTGAAATCGATCTTGCTCTTCTTCAAGAAGACGCAGAGAAAGCACTGGCTG
AAAACGTTGAAGTAATGACAGAAGCATTAGAACCAGCATTCGCAACAGGCAACTACCAAGAAGCCCTAAGCAAGCTTGCT
GATCTACGTGAACCTGTTGATGCATTCTTCGATAACGTAATGGTTATGGCTGATGACGAAGCGCTTAAGAAGAACCGTCT
AACGCTACTGAACAACCTACGTAACCTGTTCCTACAGATTGCTGATATTTCTTTACTACAGAAGTAA

Upstream 100 bases:

>100_bases
ACATCCTTCGTATCCGCAACCTGACTAAAGCTGTTGCAGAGGCGTACTACGCGTCACGTGAAGCGCTTGGCTTCCCAATG
TGTAAGAAGGATAAATAATC

Downstream 100 bases:

>100_bases
AACCAATTTGGAATAAATATTAAGGGGGAAGCTTTGGCTTCTCCCTTTTTTTTATCCAAAGCTAGTGTAAGCTCTATCTG
GTTGAATATTATTGTAGATA

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS

Number of amino acids: Translated: 688; Mature: 687

Protein sequence:

>688_residues
MAKNFLIELGTEELPPTALRSLAEAFASNFEAGLKTAELSHEGIKWYAAPRRLALKVTALAEGQADKVVEKRGPAISVAF
DAEGNATKAAQGWARGNGITVEQADRLKTDKGEWLLFKQEVAGKPVQELVMDIAAKALAGLPIPKAMRWGNSDIQFIRPV
KTLTVLLGDELVEGKILGVASARTIRGHRFMGEQEFTIDSADQYPAILEERGKVMADYDARKAIILADAKKAADAVGGIA
DLEDDLVEEVTSLVEWPVVLTAKFEQVFLKVPSEALVYTMKGDQKYFPVYDADKNLLPNFIFVSNIESKEPRHVIEGNEK
VVRPRLADAEFFFNTDRKRPLIDRLAELDQAIFQKQLGTIKDKTDRITELAGYIAEQIDADVEKSKRAGLLAKCDLMTSM
VFEFTDTQGVMGMHYATHDGEDEQVALALYEQYMPRFAGDTLPSTGISSAVAMADKLDTIVGIFGIGQAPKGSDPFALRR
ASLGVLRIIVENGYNLDLTDLIGKAKELLGDKLTNENVEADVIDFMLGRFRAWYQDAGFSVDIIQAVLARRPTKPADFDQ
RVKAVSHFRELEAAEALAAANKRVGNILAKFDGELPAEIDLALLQEDAEKALAENVEVMTEALEPAFATGNYQEALSKLA
DLREPVDAFFDNVMVMADDEALKKNRLTLLNNLRNLFLQIADISLLQK

Sequences:

>Translated_688_residues
MAKNFLIELGTEELPPTALRSLAEAFASNFEAGLKTAELSHEGIKWYAAPRRLALKVTALAEGQADKVVEKRGPAISVAF
DAEGNATKAAQGWARGNGITVEQADRLKTDKGEWLLFKQEVAGKPVQELVMDIAAKALAGLPIPKAMRWGNSDIQFIRPV
KTLTVLLGDELVEGKILGVASARTIRGHRFMGEQEFTIDSADQYPAILEERGKVMADYDARKAIILADAKKAADAVGGIA
DLEDDLVEEVTSLVEWPVVLTAKFEQVFLKVPSEALVYTMKGDQKYFPVYDADKNLLPNFIFVSNIESKEPRHVIEGNEK
VVRPRLADAEFFFNTDRKRPLIDRLAELDQAIFQKQLGTIKDKTDRITELAGYIAEQIDADVEKSKRAGLLAKCDLMTSM
VFEFTDTQGVMGMHYATHDGEDEQVALALYEQYMPRFAGDTLPSTGISSAVAMADKLDTIVGIFGIGQAPKGSDPFALRR
ASLGVLRIIVENGYNLDLTDLIGKAKELLGDKLTNENVEADVIDFMLGRFRAWYQDAGFSVDIIQAVLARRPTKPADFDQ
RVKAVSHFRELEAAEALAAANKRVGNILAKFDGELPAEIDLALLQEDAEKALAENVEVMTEALEPAFATGNYQEALSKLA
DLREPVDAFFDNVMVMADDEALKKNRLTLLNNLRNLFLQIADISLLQK
>Mature_687_residues
AKNFLIELGTEELPPTALRSLAEAFASNFEAGLKTAELSHEGIKWYAAPRRLALKVTALAEGQADKVVEKRGPAISVAFD
AEGNATKAAQGWARGNGITVEQADRLKTDKGEWLLFKQEVAGKPVQELVMDIAAKALAGLPIPKAMRWGNSDIQFIRPVK
TLTVLLGDELVEGKILGVASARTIRGHRFMGEQEFTIDSADQYPAILEERGKVMADYDARKAIILADAKKAADAVGGIAD
LEDDLVEEVTSLVEWPVVLTAKFEQVFLKVPSEALVYTMKGDQKYFPVYDADKNLLPNFIFVSNIESKEPRHVIEGNEKV
VRPRLADAEFFFNTDRKRPLIDRLAELDQAIFQKQLGTIKDKTDRITELAGYIAEQIDADVEKSKRAGLLAKCDLMTSMV
FEFTDTQGVMGMHYATHDGEDEQVALALYEQYMPRFAGDTLPSTGISSAVAMADKLDTIVGIFGIGQAPKGSDPFALRRA
SLGVLRIIVENGYNLDLTDLIGKAKELLGDKLTNENVEADVIDFMLGRFRAWYQDAGFSVDIIQAVLARRPTKPADFDQR
VKAVSHFRELEAAEALAAANKRVGNILAKFDGELPAEIDLALLQEDAEKALAENVEVMTEALEPAFATGNYQEALSKLAD
LREPVDAFFDNVMVMADDEALKKNRLTLLNNLRNLFLQIADISLLQK

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1789982, Length=686, Percent_Identity=68.5131195335277, Blast_Score=941, Evalue=0.0,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYGB_VIBSL (B7VGK6)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002415741.1
- GeneID:   7159729
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_0032
- HOGENOM:   HBG586599
- OMA:   FIRPVHT
- ProtClustDB:   PRK01233
- GO:   GO:0005737
- HAMAP:   MF_00255
- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311
- PRINTS:   PR01045
- SMART:   SM00836
- TIGRFAMs:   TIGR00211

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f

EC number: =6.1.1.14

Molecular weight: Translated: 75785; Mature: 75654

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKNFLIELGTEELPPTALRSLAEAFASNFEAGLKTAELSHEGIKWYAAPRRLALKVTAL
CCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHCCCCCEEEECCCEEEEEEEEC
AEGQADKVVEKRGPAISVAFDAEGNATKAAQGWARGNGITVEQADRLKTDKGEWLLFKQE
CCCCHHHHHHHCCCEEEEEEECCCCCCHHHCCCCCCCCCEEECHHHCCCCCCCEEEEEHH
VAGKPVQELVMDIAAKALAGLPIPKAMRWGNSDIQFIRPVKTLTVLLGDELVEGKILGVA
HCCCCHHHHHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHHHCCHHHCCEEEEEE
SARTIRGHRFMGEQEFTIDSADQYPAILEERGKVMADYDARKAIILADAKKAADAVGGIA
CCHHHCCHHCCCCCCCCCCCCCCCHHHHHHCCCEEECCCCCEEEEEECCHHHHHHHCCHH
DLEDDLVEEVTSLVEWPVVLTAKFEQVFLKVPSEALVYTMKGDQKYFPVYDADKNLLPNF
HHHHHHHHHHHHHHHCCHHEEHHHHHHHHHCCCCCEEEEEECCCEECCEECCCCCCCCCE
IFVSNIESKEPRHVIEGNEKVVRPRLADAEFFFNTDRKRPLIDRLAELDQAIFQKQLGTI
EEEECCCCCCCCHHHCCCCCEECCCCCCCHHEECCCCCCHHHHHHHHHHHHHHHHHHCCC
KDKTDRITELAGYIAEQIDADVEKSKRAGLLAKCDLMTSMVFEFTDTQGVMGMHYATHDG
CCHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCC
EDEQVALALYEQYMPRFAGDTLPSTGISSAVAMADKLDTIVGIFGIGQAPKGSDPFALRR
CCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
ASLGVLRIIVENGYNLDLTDLIGKAKELLGDKLTNENVEADVIDFMLGRFRAWYQDAGFS
HHHHEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCC
VDIIQAVLARRPTKPADFDQRVKAVSHFRELEAAEALAAANKRVGNILAKFDGELPAEID
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
LALLQEDAEKALAENVEVMTEALEPAFATGNYQEALSKLADLREPVDAFFDNVMVMADDE
HHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCH
ALKKNRLTLLNNLRNLFLQIADISLLQK
HHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AKNFLIELGTEELPPTALRSLAEAFASNFEAGLKTAELSHEGIKWYAAPRRLALKVTAL
CCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHCCCCCEEEECCCEEEEEEEEC
AEGQADKVVEKRGPAISVAFDAEGNATKAAQGWARGNGITVEQADRLKTDKGEWLLFKQE
CCCCHHHHHHHCCCEEEEEEECCCCCCHHHCCCCCCCCCEEECHHHCCCCCCCEEEEEHH
VAGKPVQELVMDIAAKALAGLPIPKAMRWGNSDIQFIRPVKTLTVLLGDELVEGKILGVA
HCCCCHHHHHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHHHCCHHHCCEEEEEE
SARTIRGHRFMGEQEFTIDSADQYPAILEERGKVMADYDARKAIILADAKKAADAVGGIA
CCHHHCCHHCCCCCCCCCCCCCCCHHHHHHCCCEEECCCCCEEEEEECCHHHHHHHCCHH
DLEDDLVEEVTSLVEWPVVLTAKFEQVFLKVPSEALVYTMKGDQKYFPVYDADKNLLPNF
HHHHHHHHHHHHHHHCCHHEEHHHHHHHHHCCCCCEEEEEECCCEECCEECCCCCCCCCE
IFVSNIESKEPRHVIEGNEKVVRPRLADAEFFFNTDRKRPLIDRLAELDQAIFQKQLGTI
EEEECCCCCCCCHHHCCCCCEECCCCCCCHHEECCCCCCHHHHHHHHHHHHHHHHHHCCC
KDKTDRITELAGYIAEQIDADVEKSKRAGLLAKCDLMTSMVFEFTDTQGVMGMHYATHDG
CCHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCC
EDEQVALALYEQYMPRFAGDTLPSTGISSAVAMADKLDTIVGIFGIGQAPKGSDPFALRR
CCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
ASLGVLRIIVENGYNLDLTDLIGKAKELLGDKLTNENVEADVIDFMLGRFRAWYQDAGFS
HHHHEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCC
VDIIQAVLARRPTKPADFDQRVKAVSHFRELEAAEALAAANKRVGNILAKFDGELPAEID
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
LALLQEDAEKALAENVEVMTEALEPAFATGNYQEALSKLADLREPVDAFFDNVMVMADDE
HHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCH
ALKKNRLTLLNNLRNLFLQIADISLLQK
HHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA