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The map label for this gene is gyrB [H]

Identifier: 218708101

GI number: 218708101

Start: 11273

End: 13690

Strand: Direct

Name: gyrB [H]

Synonym: VS_0013

Alternate gene names: 218708101

Gene position: 11273-13690 (Clockwise)

Preceding gene: 218708100

Following gene: 218708102

Centisome position: 0.34

GC content: 45.66

Gene sequence:

>2418_bases
ATGTCAGATAATTACGATTCATCGAGTATTAAAGTACTGAAGGGTCTGGATGCGGTACGTAAGCGTCCTGGAATGTACAT
TGGCGACACGGATGATGGTACCGGTCTGCACCACATGGTTTTTGAGGTGGTAGATAACTCTATTGATGAAGCGCTAGCTG
GTCACTGTAATGACATCATTGTAACTATCCATGAAGATAGCTCAGTGTCTGTAAGCGATGACGGCCGTGGTATTCCAACA
GAATTGCACCCAGAAGAAAAAGTATCAGCAGCAGAAGTAATCATGACGGTTCTTCACGCTGGTGGTAAGTTCGATGATAA
CTCGTACAAGGTATCTGGTGGTCTGCACGGTGTTGGTGTTTCAGTAGTTAACGCACTTTCTAAGCAAGTTACTCTTACTA
TCCACCGCGGTGGCAAAATCCACACTCAAACCTACAGCCACGGTGAGCCTCAAGCGCCACTAGCGGTTATCGGTGACACG
GATAAAACCGGTACAGAGATTCGTTTCTGGCCGAGTGAAGAAACCTTCACTAACATCGAGTTCCATTACGACATTTTAGC
TAAGCGTCTACGTGAGCTATCATTCTTGAACTCAGGTGTATCAATTAAGCTAATTGATAAGCGCGAAGAAGACAAAATGG
ATCACTTCGAATACGAAGGCGGTATCCAAGCGTTTGTTGAACACCTTAATACCAACAAAACACCAATCATCCAAAAGATT
TTCCACTTTGATTCAGAGCGTGATGACGGCATTACCGTTGAAGTTGCAATGCAGTGGAACGATGGTTATCAAGAAAACAT
CTATTGTTTCACCAACAACATCCCTCAACGCGATGGTGGTGCTCACTTAGCGGGTTTCCGTGCTGCACTAACGCGTACCT
TGAACAGCTTCATGGATAAAGAAGGCTTCTCGAAGAAAGCGAAGACTTCAACATCCGGTGATGATGCTCGTGAAGGTTTG
ACGGCGATTGTTTCGGTGAAAGTGCCAGATCCTAAGTTCTCAAGCCAAACGAAAGACAAGCTGGTTTCTTCAGAAGTGAA
GTCTGCCGTTGAGTCTACTATGGGTGAGAAGCTATCTGAGTTCCTAATTGAGAACCCAGGTGAAGCGAAAATCGTTTGTT
CTAAAATTATCGATGCAGCACGTGCTCGTGATGCAGCGCGTAAAGCTCGTGAGATGACTCGTCGTAAAGGCGCATTAGAT
TTAGCGGGTCTTCCAGGTAAACTAGCTGACTGTCAGGAAAAAGATCCTGCACAGTCTGAACTATATATTGTGGAAGGAGA
CTCTGCTGGCGGATCAGCTAAGCAGGGGCGTAACCGTAAGAACCAAGCAATCCTACCGCTTAAAGGTAAAATTCTTAACG
TAGAGAAAGCGCGTTTCGATAAAATGCTGTCTTCTCAAGAAGTTGCAACGCTAATCACAGCCCTTGGTTGTGGTATTGGT
CGTGATGAATACAACCCAGATAAACTGCGTTACCACAACATCATCATCATGACCGATGCCGATGTCGATGGTTCTCACAT
CCGTACACTGCTACTGACGTTCTTCTACCGTCAAATGCCTGAGCTGATTGAACGTGGTTACATCTACATTGCTCAACCGC
CACTTTACAAAGTGAAGAAGGGTAAGCAAGAGCAGTACATCAAAGATGAAGATGCGATGAACCAGTATCAAGTATCTTTG
GCTTTAGACAATGCAGAACTGCACGTAAATCCTGAAGCACCTGCGTTTGCCGGTGAAGGCTTAGAGAAGCTTGTTCAGCA
ATATAATGCAGGCATCAAGCTCGTTGATCGTATGAGCCGCCGCTACCCACGCGCATTGGTTCACGAACTGATTTACGTTC
CTCGCCTAACCGCTGAGCAGTGTCATGATGATGCGGTGGTTGAAGCTTGGGGTAAGCAGCTAGTAGAGCAGCTAAACGGT
AAAGAAGCGGGTGCAAGCCAATACAGCCTTGAAGTTGAAAAGCACGAAGAGCTAGGTTTAAGCGTTCCTAAGATTGTGGT
TCGTACTCATGGTGTAACTCATGAGCACGTGCTGAGCATTGATCTGATTAACTCGAAAGAGTTTGGCAAGCTAGCGGATC
TTTCTGAAGCACTTGATGGCCTGATTGAAGAAGGTGCTTACATTAAGCGTGGCGAGCGTACTCAAGAAGTTTCTAGCTTC
GTTGAAGCTCTGAACTGGTTAGTGAAAGAGTCTCGTCGTGGCCTGAGCCTACAACGATACAAAGGTCTAGGTGAGATGAA
CCCTGATCAGCTTTGGGAAACAACAATGGATCCTGAAACTCGTCGTATGATGCAAGTAACGATTGAAGATGCTGTTGGTG
CTGATCTGTTGTTTACGACACTAATGGGTGACCAAGTTGAACCTCGTCGTAACTTCATCGAAGAAAACGCACTTAAAGTA
GCTAACCTAGACGTTTAG

Upstream 100 bases:

>100_bases
TTACCGCTGATCAGATTGCAGATATGCATGACGAAAATAGCAGGATGTTTCATGTGGAACATGGCAAAATAGAGCAAGGA
TAATTAGTCAGAGAGTAACT

Downstream 100 bases:

>100_bases
TATCTTTGATACTTCGCTCTTTCGCTTAATTTCTGTGTCAGAGGTGCTCACATACATTCGTATGCTCCGCGCCTCTTCCT
TGAACTAAAACGAAATAGTT

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 805; Mature: 804

Protein sequence:

>805_residues
MSDNYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNSIDEALAGHCNDIIVTIHEDSSVSVSDDGRGIPT
ELHPEEKVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSKQVTLTIHRGGKIHTQTYSHGEPQAPLAVIGDT
DKTGTEIRFWPSEETFTNIEFHYDILAKRLRELSFLNSGVSIKLIDKREEDKMDHFEYEGGIQAFVEHLNTNKTPIIQKI
FHFDSERDDGITVEVAMQWNDGYQENIYCFTNNIPQRDGGAHLAGFRAALTRTLNSFMDKEGFSKKAKTSTSGDDAREGL
TAIVSVKVPDPKFSSQTKDKLVSSEVKSAVESTMGEKLSEFLIENPGEAKIVCSKIIDAARARDAARKAREMTRRKGALD
LAGLPGKLADCQEKDPAQSELYIVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITALGCGIG
RDEYNPDKLRYHNIIIMTDADVDGSHIRTLLLTFFYRQMPELIERGYIYIAQPPLYKVKKGKQEQYIKDEDAMNQYQVSL
ALDNAELHVNPEAPAFAGEGLEKLVQQYNAGIKLVDRMSRRYPRALVHELIYVPRLTAEQCHDDAVVEAWGKQLVEQLNG
KEAGASQYSLEVEKHEELGLSVPKIVVRTHGVTHEHVLSIDLINSKEFGKLADLSEALDGLIEEGAYIKRGERTQEVSSF
VEALNWLVKESRRGLSLQRYKGLGEMNPDQLWETTMDPETRRMMQVTIEDAVGADLLFTTLMGDQVEPRRNFIEENALKV
ANLDV

Sequences:

>Translated_805_residues
MSDNYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNSIDEALAGHCNDIIVTIHEDSSVSVSDDGRGIPT
ELHPEEKVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSKQVTLTIHRGGKIHTQTYSHGEPQAPLAVIGDT
DKTGTEIRFWPSEETFTNIEFHYDILAKRLRELSFLNSGVSIKLIDKREEDKMDHFEYEGGIQAFVEHLNTNKTPIIQKI
FHFDSERDDGITVEVAMQWNDGYQENIYCFTNNIPQRDGGAHLAGFRAALTRTLNSFMDKEGFSKKAKTSTSGDDAREGL
TAIVSVKVPDPKFSSQTKDKLVSSEVKSAVESTMGEKLSEFLIENPGEAKIVCSKIIDAARARDAARKAREMTRRKGALD
LAGLPGKLADCQEKDPAQSELYIVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITALGCGIG
RDEYNPDKLRYHNIIIMTDADVDGSHIRTLLLTFFYRQMPELIERGYIYIAQPPLYKVKKGKQEQYIKDEDAMNQYQVSL
ALDNAELHVNPEAPAFAGEGLEKLVQQYNAGIKLVDRMSRRYPRALVHELIYVPRLTAEQCHDDAVVEAWGKQLVEQLNG
KEAGASQYSLEVEKHEELGLSVPKIVVRTHGVTHEHVLSIDLINSKEFGKLADLSEALDGLIEEGAYIKRGERTQEVSSF
VEALNWLVKESRRGLSLQRYKGLGEMNPDQLWETTMDPETRRMMQVTIEDAVGADLLFTTLMGDQVEPRRNFIEENALKV
ANLDV
>Mature_804_residues
SDNYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNSIDEALAGHCNDIIVTIHEDSSVSVSDDGRGIPTE
LHPEEKVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSKQVTLTIHRGGKIHTQTYSHGEPQAPLAVIGDTD
KTGTEIRFWPSEETFTNIEFHYDILAKRLRELSFLNSGVSIKLIDKREEDKMDHFEYEGGIQAFVEHLNTNKTPIIQKIF
HFDSERDDGITVEVAMQWNDGYQENIYCFTNNIPQRDGGAHLAGFRAALTRTLNSFMDKEGFSKKAKTSTSGDDAREGLT
AIVSVKVPDPKFSSQTKDKLVSSEVKSAVESTMGEKLSEFLIENPGEAKIVCSKIIDAARARDAARKAREMTRRKGALDL
AGLPGKLADCQEKDPAQSELYIVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITALGCGIGR
DEYNPDKLRYHNIIIMTDADVDGSHIRTLLLTFFYRQMPELIERGYIYIAQPPLYKVKKGKQEQYIKDEDAMNQYQVSLA
LDNAELHVNPEAPAFAGEGLEKLVQQYNAGIKLVDRMSRRYPRALVHELIYVPRLTAEQCHDDAVVEAWGKQLVEQLNGK
EAGASQYSLEVEKHEELGLSVPKIVVRTHGVTHEHVLSIDLINSKEFGKLADLSEALDGLIEEGAYIKRGERTQEVSSFV
EALNWLVKESRRGLSLQRYKGLGEMNPDQLWETTMDPETRRMMQVTIEDAVGADLLFTTLMGDQVEPRRNFIEENALKVA
NLDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=552, Percent_Identity=26.4492753623188, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI19913406, Length=563, Percent_Identity=25.7548845470693, Blast_Score=124, Evalue=3e-28,
Organism=Escherichia coli, GI48994957, Length=806, Percent_Identity=76.4267990074442, Blast_Score=1253, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=554, Percent_Identity=38.8086642599278, Blast_Score=378, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17535065, Length=539, Percent_Identity=27.8293135435993, Blast_Score=147, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI212645845, Length=524, Percent_Identity=23.6641221374046, Blast_Score=123, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI212645657, Length=153, Percent_Identity=33.9869281045752, Blast_Score=77, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6324241, Length=591, Percent_Identity=24.5346869712352, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI17136538, Length=669, Percent_Identity=25.4110612855007, Blast_Score=151, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 89539; Mature: 89408

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDNYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNSIDEALAGHCNDII
CCCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEE
VTIHEDSSVSVSDDGRGIPTELHPEEKVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGV
EEEECCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHH
SVVNALSKQVTLTIHRGGKIHTQTYSHGEPQAPLAVIGDTDKTGTEIRFWPSEETFTNIE
HHHHHHCCEEEEEEECCCEEEECCCCCCCCCCCEEEECCCCCCCCEEEEECCCCCEEEEE
FHYDILAKRLRELSFLNSGVSIKLIDKREEDKMDHFEYEGGIQAFVEHLNTNKTPIIQKI
EHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHCCHHHHHHHHHCCCCCHHHHHH
FHFDSERDDGITVEVAMQWNDGYQENIYCFTNNIPQRDGGAHLAGFRAALTRTLNSFMDK
HHCCCCCCCCEEEEEEEEECCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHH
EGFSKKAKTSTSGDDAREGLTAIVSVKVPDPKFSSQTKDKLVSSEVKSAVESTMGEKLSE
CCCCCCCCCCCCCCHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FLIENPGEAKIVCSKIIDAARARDAARKAREMTRRKGALDLAGLPGKLADCQEKDPAQSE
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCE
LYIVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITALGCGIG
EEEEECCCCCCCCCCCCCCCCCEEEECCCCEECHHHHHHHHHHHHHHHHHHHHHHHCCCC
RDEYNPDKLRYHNIIIMTDADVDGSHIRTLLLTFFYRQMPELIERGYIYIAQPPLYKVKK
CCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHC
GKQEQYIKDEDAMNQYQVSLALDNAELHVNPEAPAFAGEGLEKLVQQYNAGIKLVDRMSR
CCHHHHCCCHHHHCEEEEEEEEECCEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHH
RYPRALVHELIYVPRLTAEQCHDDAVVEAWGKQLVEQLNGKEAGASQYSLEVEKHEELGL
HCHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHCCC
SVPKIVVRTHGVTHEHVLSIDLINSKEFGKLADLSEALDGLIEEGAYIKRGERTQEVSSF
CCCHHHHHHCCCCHHHEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEECCCHHHHHHHH
VEALNWLVKESRRGLSLQRYKGLGEMNPDQLWETTMDPETRRMMQVTIEDAVGADLLFTT
HHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHCCCCHHHHHHHHEEHHHHCCHHHHHHH
LMGDQVEPRRNFIEENALKVANLDV
HHCCCCCHHHHHHHHCCEEEECCCC
>Mature Secondary Structure 
SDNYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNSIDEALAGHCNDII
CCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEE
VTIHEDSSVSVSDDGRGIPTELHPEEKVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGV
EEEECCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHH
SVVNALSKQVTLTIHRGGKIHTQTYSHGEPQAPLAVIGDTDKTGTEIRFWPSEETFTNIE
HHHHHHCCEEEEEEECCCEEEECCCCCCCCCCCEEEECCCCCCCCEEEEECCCCCEEEEE
FHYDILAKRLRELSFLNSGVSIKLIDKREEDKMDHFEYEGGIQAFVEHLNTNKTPIIQKI
EHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHCCHHHHHHHHHCCCCCHHHHHH
FHFDSERDDGITVEVAMQWNDGYQENIYCFTNNIPQRDGGAHLAGFRAALTRTLNSFMDK
HHCCCCCCCCEEEEEEEEECCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHH
EGFSKKAKTSTSGDDAREGLTAIVSVKVPDPKFSSQTKDKLVSSEVKSAVESTMGEKLSE
CCCCCCCCCCCCCCHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FLIENPGEAKIVCSKIIDAARARDAARKAREMTRRKGALDLAGLPGKLADCQEKDPAQSE
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCE
LYIVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITALGCGIG
EEEEECCCCCCCCCCCCCCCCCEEEECCCCEECHHHHHHHHHHHHHHHHHHHHHHHCCCC
RDEYNPDKLRYHNIIIMTDADVDGSHIRTLLLTFFYRQMPELIERGYIYIAQPPLYKVKK
CCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHC
GKQEQYIKDEDAMNQYQVSLALDNAELHVNPEAPAFAGEGLEKLVQQYNAGIKLVDRMSR
CCHHHHCCCHHHHCEEEEEEEEECCEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHH
RYPRALVHELIYVPRLTAEQCHDDAVVEAWGKQLVEQLNGKEAGASQYSLEVEKHEELGL
HCHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHCCC
SVPKIVVRTHGVTHEHVLSIDLINSKEFGKLADLSEALDGLIEEGAYIKRGERTQEVSSF
CCCHHHHHHCCCCHHHEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEECCCHHHHHHHH
VEALNWLVKESRRGLSLQRYKGLGEMNPDQLWETTMDPETRRMMQVTIEDAVGADLLFTT
HHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHCCCCHHHHHHHHEEHHHHCCHHHHHHH
LMGDQVEPRRNFIEENALKVANLDV
HHCCCCCHHHHHHHHCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12620739; 9464408 [H]