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The map label for this gene is uidA [H]

Identifier: 218705117

GI number: 218705117

Start: 1929037

End: 1930848

Strand: Reverse

Name: uidA [H]

Synonym: ECUMN_1906

Alternate gene names: 218705117

Gene position: 1930848-1929037 (Counterclockwise)

Preceding gene: 218705118

Following gene: 218705116

Centisome position: 37.12

GC content: 52.1

Gene sequence:

>1812_bases
ATGTTACGTCCTGTAGAAACCCCAACCCGTGAAATCAAAAAACTCGACGGCCTGTGGGCATTCAGTCTGGATCGCGAAAA
CTGTGGAATTGATCAGCGTTGGTGGGAAAGCGTGTTACAAGAAAGCCGGGCAATTGCTGTGCCGGGCAGTTTTAACGATC
AGTTCGCCGATGCAGATATTCGTAATTATGTGGGCAACGTCTGGTATCAGCGCGAAGTCTTTATACCGAAAGGTTGGGCG
GGCCAGCGTATCGTGCTGCGTTTCGATGCGGTCACTCATTACGGCAAAGTGTGGGTAAATAATCAGGAAGTGATGGAGCA
TCAGGGCGGCTATACGCCATTTGAAGCCGATGTCACGCCGTATGTTATTGCCGGGAAAAGTGTACGTATCACCGTTTGTG
TGAACAATGAACTGAACTGGCAGACTATCCCGCCGGGAATGGTGATTACCGATGAAAACGGCAAGAAAAAGCAGTCTTAC
TTCCATGATTTCTTTAACTATGCCGGGATCCATCGCAGCGTAATGCTCTACACCACGCCGAACACCTGGGTGGACGATAT
CACCGTGGTGACGCATGTCGCGCAAGACTGTAACCACGCGTCTGTTGACTGGCAGGTGGTGGCCAATGGTGATGTCAGCG
TTGAACTGCGTGATGCGGATCAACAGGTGGTTGCAACTGGACAAGGCACCAGCGGGACTTTGCAAGTGGTGAATCCGCAC
CTCTGGCAACCGGGTGAAGGTTATCTCTATGAACTGTGCGTCACAGCCAAAAGCCAGACAGAGTGTGATATCTACCCGCT
GCGCGTCGGCATCCGGTCAGTGGCAGTGAAGGGCGAGCAGTTCCTGATTAACCACAAACCGTTCTATTTTACTGGCTTTG
GTCGTCATGAAGATGCGGATTTGCGTGGCAAAGGATTCGATAACGTGCTGATGGTGCACGATCACGCATTAATGGACTGG
ATTGGGGCCAACTCCTACCGTACCTCGCATTACCCTTACGCTGAAGAGATGCTCGACTGGGCAGATGAACATGGCATCGT
GGTGATTGATGAAACTGCAGCTGTAGGCTTTAACCTCTCTTTAGGCATTGGTTTCGAAGCGGGCATCAAGCCGAAAGAAC
TGTACAGCGAAGAGGCAGTCAACGGGGAAACTCAGCAGGCGCACTTACAGGCGATTAAAGAGCTGATTGCGCGTGACAAA
AACCACCCAAGCGTGGTGATGTGGAGTATTGCCAACGAACCGGATACCCGTCCGCAAGGTGCACGGGAATATTTCGCGCC
ACTGGCGGAAGCAACGCGTAAACTCGACCCGACGCGTCCGATCACCTGTGTCAATGTAATGTTCTGCGACGCTCACACCG
ATACCATCAGCGATCTCTTTGATGTGCTGTGCCTGAACCGTTATTACGGTTGGTATGTCCAAAGCGGCGATTTGGAAACG
GCAGAGAAGGTACTGGAAAAAGAACTGCTGGCCTGGCAGGAGAAACTGCATCAGCCGATTATCATCACCGAATACGGCGT
GGATACGTTAGCCGGGCTGCACTCAATGTACACCGACATGTGGAGTGAAGAGTATCAGTGTGCATGGCTGGATATGTATC
ACCGCGTCTTTGATCGCGTCAGCGCCGTCGTCGGTGAACAGGTATGGAATTTCGCCGATTTTGCGACCTCACAAGGCATA
TTGCGCGTTGGCGGTAACAAGAAAGGGATCTTCACCCGCGACCGCAAACCGAAGTCGGCGGCTTTTCTGCTGCAAAAACG
CTGGACTGGCATGAACTTCGGTGAAAAACCGCAGCAGGGAGGCAAACAATGA

Upstream 100 bases:

>100_bases
CTTCCATGGGTTTCTCACAGATAACTGTGTGCAACACAGAATTGGTTAACTAATCAGATTAAAGGTTGACCAGTATTTTT
ATCTTAATGAGGAGTCCCTT

Downstream 100 bases:

>100_bases
ATCAACAACTCTCCTGGCGCACCATCGTCGGCTACAGCCTCGGTGACGTCGCCAATAACTTCGCCTTCGCAATGGGGGCG
CTCTTCCTGTTGAGTTACTA

Product: beta-D-glucuronidase

Products: NA

Alternate protein names: GUS; Beta-D-glucuronoside glucuronosohydrolase [H]

Number of amino acids: Translated: 603; Mature: 603

Protein sequence:

>603_residues
MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESVLQESRAIAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWA
GQRIVLRFDAVTHYGKVWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQVVATGQGTSGTLQVVNPH
LWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDW
IGANSYRTSHYPYAEEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGIKPKELYSEEAVNGETQQAHLQAIKELIARDK
NHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISDLFDVLCLNRYYGWYVQSGDLET
AEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGI
LRVGGNKKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ

Sequences:

>Translated_603_residues
MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESVLQESRAIAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWA
GQRIVLRFDAVTHYGKVWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQVVATGQGTSGTLQVVNPH
LWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDW
IGANSYRTSHYPYAEEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGIKPKELYSEEAVNGETQQAHLQAIKELIARDK
NHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISDLFDVLCLNRYYGWYVQSGDLET
AEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGI
LRVGGNKKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ
>Mature_603_residues
MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESVLQESRAIAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWA
GQRIVLRFDAVTHYGKVWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQVVATGQGTSGTLQVVNPH
LWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDW
IGANSYRTSHYPYAEEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGIKPKELYSEEAVNGETQQAHLQAIKELIARDK
NHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISDLFDVLCLNRYYGWYVQSGDLET
AEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGI
LRVGGNKKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ

Specific function: Unknown

COG id: COG3250

COG function: function code G; Beta-galactosidase/beta-glucuronidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 2 family [H]

Homologues:

Organism=Homo sapiens, GI268834192, Length=636, Percent_Identity=45.125786163522, Blast_Score=506, Evalue=1e-143,
Organism=Escherichia coli, GI1787903, Length=603, Percent_Identity=99.5024875621891, Blast_Score=1258, Evalue=0.0,
Organism=Escherichia coli, GI1786539, Length=481, Percent_Identity=26.1954261954262, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI48994920, Length=390, Percent_Identity=26.1538461538462, Blast_Score=106, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17510825, Length=617, Percent_Identity=36.3047001620746, Blast_Score=388, Evalue=1e-108,
Organism=Drosophila melanogaster, GI62471735, Length=624, Percent_Identity=43.4294871794872, Blast_Score=486, Evalue=1e-137,
Organism=Drosophila melanogaster, GI19922582, Length=624, Percent_Identity=43.4294871794872, Blast_Score=486, Evalue=1e-137,
Organism=Drosophila melanogaster, GI24655438, Length=624, Percent_Identity=43.4294871794872, Blast_Score=484, Evalue=1e-137,
Organism=Drosophila melanogaster, GI24651745, Length=633, Percent_Identity=39.1785150078989, Blast_Score=438, Evalue=1e-123,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008979
- InterPro:   IPR006101
- InterPro:   IPR013812
- InterPro:   IPR006104
- InterPro:   IPR006102
- InterPro:   IPR006103
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00703 Glyco_hydro_2; PF02836 Glyco_hydro_2_C; PF02837 Glyco_hydro_2_N [H]

EC number: =3.2.1.31 [H]

Molecular weight: Translated: 68503; Mature: 68503

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00719 GLYCOSYL_HYDROL_F2_1 ; PS00608 GLYCOSYL_HYDROL_F2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESVLQESRAIAVPGSFNDQFADADI
CCCCCCCCHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHCCCEEECCCCCCCCCCCCHH
RNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEVMEHQGGYTPFEADVTP
HHHHCCEEEEEEEECCCCCCCCEEEEEEECHHCCCEEEECCHHHHHHCCCCCCCCCCCCC
YVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHDFFNYAGIHRSVMLYTTP
EEEECCEEEEEEEECCCCCCEECCCCEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEECC
NTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQVVATGQGTSGTLQVVNPH
CCCHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEECCCCEEEEECCCCCCEEEEECCC
LWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQFLINHKPFYFTGFGRHEDAD
CCCCCCCEEEEEEEECCCCCCCCEEEEEECHHHHEECCCEEEEECCCEEEECCCCCCCCC
LRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDWADEHGIVVIDETAAVGFNLS
CCCCCCCCEEEEECHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCEEEEEEE
LGIGFEAGIKPKELYSEEAVNGETQQAHLQAIKELIARDKNHPSVVMWSIANEPDTRPQG
EECCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCC
AREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISDLFDVLCLNRYYGWYVQSGDLET
HHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHCCEEEECCCHHH
AEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRV
HHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
SAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQG
HHHHHHHHHHHHHHHCCCCEEEECCCCCCCEECCCCCCHHHHHHHHHCCCCCCCCCCCCC
GKQ
CCC
>Mature Secondary Structure
MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESVLQESRAIAVPGSFNDQFADADI
CCCCCCCCHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHCCCEEECCCCCCCCCCCCHH
RNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEVMEHQGGYTPFEADVTP
HHHHCCEEEEEEEECCCCCCCCEEEEEEECHHCCCEEEECCHHHHHHCCCCCCCCCCCCC
YVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHDFFNYAGIHRSVMLYTTP
EEEECCEEEEEEEECCCCCCEECCCCEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEECC
NTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQVVATGQGTSGTLQVVNPH
CCCHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEECCCCEEEEECCCCCCEEEEECCC
LWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQFLINHKPFYFTGFGRHEDAD
CCCCCCCEEEEEEEECCCCCCCCEEEEEECHHHHEECCCEEEEECCCEEEECCCCCCCCC
LRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDWADEHGIVVIDETAAVGFNLS
CCCCCCCCEEEEECHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCEEEEEEE
LGIGFEAGIKPKELYSEEAVNGETQQAHLQAIKELIARDKNHPSVVMWSIANEPDTRPQG
EECCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCC
AREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISDLFDVLCLNRYYGWYVQSGDLET
HHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHCCEEEECCCHHH
AEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRV
HHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
SAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQG
HHHHHHHHHHHHHHHCCCCEEEECCCCCCCEECCCCCCHHHHHHHHHCCCCCCCCCCCCC
GKQ
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3534890; 8125312; 2103475; 9097039; 9278503 [H]