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The map label for this gene is citE [H]

Identifier: 218703950

GI number: 218703950

Start: 763082

End: 763990

Strand: Reverse

Name: citE [H]

Synonym: ECUMN_0709

Alternate gene names: 218703950

Gene position: 763990-763082 (Counterclockwise)

Preceding gene: 218703951

Following gene: 218703949

Centisome position: 14.69

GC content: 57.32

Gene sequence:

>909_bases
ATGATTTCCGCTTCGCTGCAACAACGTAAAACTCGCACCCGCCGCAGCATGTTGTTTGTGCCTGGTGCCAATGCCGCGAT
GGTCAGCAACTCCTTCATCTACCCGGCTGATGCCCTGATGTTTGACCTCGAAGACTCCGTGGCATTACGTGAAAAAGACA
CCGCCCGCCGCATGGTTTACCACGCGCTGCAACATCCGCTGTATCGCGATATTGAAACCATTGTGCGTGTAAACGCGCTG
GATTCCGAATGGGGTGTTAACGACCTGGAAGCCGTCGTTCGCGGTGGTGCGGACGTTGTGCGTCTGCCGAAAACCGATAC
CGCTCAGGATGTTCTGGATATCGAAAAAGAGATCCTGCGTATCGAAAAAGCCTGTGGTCGTGAACCCGGCAGTACCGGCT
TGCTGGCGGCGATTGAATCTCCGCTGGGCATTACCCGCGCAGTGGAAATCGCTCACGCTTCCGAGCGTTTGATCGGTATC
GCCCTCGGTGCAGAAGACTATGTGCGCAACCTGCGTACAGAACGCTCCCCGGAAGGAATTGAACTGCTGTTCGCTCGCTG
CTCCATTTTGCAGGCCGCGCGCTCTGCGGGTATTCAGGCGTTCGATACCGTCTATTCCGACGCTAACAACGAAGCCGGAT
TCCTGCAAGAAGCCGCCCACATCAAACAGCTGGGCTTTGACGGCAAATCGCTGATTAACCCGCGTCAGATTGATCTGCTG
CACAACCTCTACGCACCGACCCAGAAAGAAGTGGATCACGCCCGCCGCGTCGTAGAAGCCGCTGAAGCCGCCGCTCGCGA
AGGCCTCGGCGTGGTTTCCCTGAATGGCAAGATGGTGGACGGTCCGGTTATCGATCGCGCCCGTCTGGTGCTCTCCCGCG
CAGAACTTTCCGGCATCCGCGAAGAATAA

Upstream 100 bases:

>100_bases
TCGATGACAAAGGCGCACTGGACTGCATTTTACGTGCACGACTGGAAGCCCTGCTGGCACGCGCCAGCGGTATCCCGGCT
CTGCCATGGGAGGATTGCCA

Downstream 100 bases:

>100_bases
GGCAATCAAAATGACGCAGAAAATTGAACAATCTCAACGACAAGAACGGGTAGCGGCCTGGAATCGTCGCGCTGAATGCG
ATCTTGCCGCTTTCCAGAAC

Product: citrate lyase, citryl-ACP lyase subunit beta

Products: NA

Alternate protein names: Citrase beta chain; Citrate (pro-3S)-lyase subunit beta; Citryl-CoA lyase subunit [H]

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLEDSVALREKDTARRMVYHALQHPLYRDIETIVRVNAL
DSEWGVNDLEAVVRGGADVVRLPKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
ALGAEDYVRNLRTERSPEGIELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEAAHIKQLGFDGKSLINPRQIDLL
HNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNGKMVDGPVIDRARLVLSRAELSGIREE

Sequences:

>Translated_302_residues
MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLEDSVALREKDTARRMVYHALQHPLYRDIETIVRVNAL
DSEWGVNDLEAVVRGGADVVRLPKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
ALGAEDYVRNLRTERSPEGIELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEAAHIKQLGFDGKSLINPRQIDLL
HNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNGKMVDGPVIDRARLVLSRAELSGIREE
>Mature_302_residues
MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLEDSVALREKDTARRMVYHALQHPLYRDIETIVRVNAL
DSEWGVNDLEAVVRGGADVVRLPKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
ALGAEDYVRNLRTERSPEGIELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEAAHIKQLGFDGKSLINPRQIDLL
HNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNGKMVDGPVIDRARLVLSRAELSGIREE

Specific function: Represents a citryl-ACP lyase [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. Citrate lyase beta subunit subfamily [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=310, Percent_Identity=25.1612903225806, Blast_Score=110, Evalue=2e-24,
Organism=Escherichia coli, GI87081764, Length=302, Percent_Identity=99.6688741721854, Blast_Score=608, Evalue=1e-175,
Organism=Caenorhabditis elegans, GI17557458, Length=301, Percent_Identity=27.5747508305648, Blast_Score=104, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR006475
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: =4.1.3.6; =4.1.3.34 [H]

Molecular weight: Translated: 33122; Mature: 33122

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLEDSVALREKDTARRMVY
CCCCHHHHHHHHHHHCEEEECCCCHHHHCCCCCCCCHHHEEECCCCHHHHHHHHHHHHHH
HALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRLPKTDTAQDVLDIEKEILR
HHHHCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHH
IEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGIALGAEDYVRNLRTERSPEGI
HHHHHCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHEEEEECHHHHHHHHHCCCCCCHH
ELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEAAHIKQLGFDGKSLINPRQIDLL
HHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHCCCHHHHHH
HNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNGKMVDGPVIDRARLVLSRAELSGIR
HHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEECCCHHHHHHHHHHHHHHHCCC
EE
CC
>Mature Secondary Structure
MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLEDSVALREKDTARRMVY
CCCCHHHHHHHHHHHCEEEECCCCHHHHCCCCCCCCHHHEEECCCCHHHHHHHHHHHHHH
HALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRLPKTDTAQDVLDIEKEILR
HHHHCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHH
IEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGIALGAEDYVRNLRTERSPEGI
HHHHHCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHEEEEECHHHHHHHHHCCCCCCHH
ELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEAAHIKQLGFDGKSLINPRQIDLL
HHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHCCCHHHHHH
HNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNGKMVDGPVIDRARLVLSRAELSGIR
HHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEECCCHHHHHHHHHHHHHHHCCC
EE
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]