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The map label for this gene is citF [H]

Identifier: 218703949

GI number: 218703949

Start: 761539

End: 763071

Strand: Reverse

Name: citF [H]

Synonym: ECUMN_0708

Alternate gene names: 218703949

Gene position: 763071-761539 (Counterclockwise)

Preceding gene: 218703950

Following gene: 218703948

Centisome position: 14.67

GC content: 55.84

Gene sequence:

>1533_bases
ATGACGCAGAAAATTGAACAATCTCAACGACAAGAACGGGTAGCGGCCTGGAATCGTCGCGCTGAATGCGATCTTGCCGC
TTTCCAGAACTCGCCAAAGCAAACCTACCAGGCTGAAAAAGCGCGCGATCGCAAACTGTGCGCCAACCTGGAAGAAGCGA
TTCGTCGCTCGGGTTTACAGGACGGTATGACGGTTTCCTTCCATCACGCTTTCCGTGGCGGTGACCTGACCGTCAATATG
GTGATGGACGTCATCGCGAAGATGGGCTTTAAAAACCTGACTCTGGCCTCCAGCTCCCTGAGTGATTGCCATGCGCCGCT
GGTAGAACACATTCGCCAGGGCGTGGTTACCCGCATTTATACCTCTGGCCTGCGCGGTCCACTGGCGGAAGAAATCTCCC
GTGGTCTGCTGGCGGAACCGGTGCAAATCCACTCTCACGGCGGACGTGTGCATCTGGTACAGAGCGGCGAACTGAATATA
GACGTGGCTTTCCTCGGCGTCCCGTCCTGTGATGAATTCGGTAATGCCAACGGCTACACCGGTAAAGCCTGCTGCGGCTC
CCTCGGCTATGCAATGGTTGATGCAGACAACGCGAAACAGGTTGTGATGCTCACTGAAGAACTGCTGCCTTATCCTCATA
ATCCGGCAAGCATTGAGCAAGATCAGGTTGATCTGATCGTCAAAGTTGATCGCGTTGGCGATGCCGCGAAAATCGGCGCT
GGCGCGACCCGTATGACCACTAACCCGCGCGAACTGCTTATTGCCCGTAGCGCTGCGGATGTGATTTTCAACTCAGGCTA
CTTTAAAGAAGGTTTCTCCATGCAAACCGGCACCGGCGGCGCATCGCTGGCGGTAACCCGTTTCCTGGAAGACAAAATGC
GTAGCCGCGATATTCGCGCCGACTTCGCCCTTGGCGGTATTACCGCAACGATGGTTGATCTGCACGAAAAAGGTCTGATC
CGCAAACTGCTGGATGTACAGAGCTTTGACAGCCACGCTGCACAATCGCTGGCCCGTAACCCCAATCACATCGAAATCAG
CGCCAACCAGTACGCTAACTGGGGTTCAAAAGGTGCATCGGTTGATCGTCTCGACGTGGTGGTACTGAGCGCGCTGGAAA
TTGACACCCAGTTCAACGTTAACGTGCTGACCGGCTCTGACGGCGTACTGCGTGGCGCTTCCGGTGGTCACTGCGATACC
GCGATTGCCTCTGCACTTTCCATCATCGTCGCACCGCTGGTACGCGGTCGTATTCCGACTCTGGTGGATAACGTGCTGAC
CTGCATCACCCCGGGCTCCAGTGTCGATATTCTGGTCACAGACCACGGTATCGCAGTTAACCCGGCACGTCCGGAACTGG
CAGAACGTCTGCAGGAAGCGGGGATCAAAGTGGTTTCTATTGAGTGGCTGCGTGAACGTGCGCGTCTGCTGACCGGTGAA
CCACAGCCGATTGAATTCACCGACCGCGTCGTTGCCGTTGTGCGTTACCGCGATGGCTCGGTGATCGATGTTGTGCATCA
GGTGAAGGAATAA

Upstream 100 bases:

>100_bases
CTGAATGGCAAGATGGTGGACGGTCCGGTTATCGATCGCGCCCGTCTGGTGCTCTCCCGCGCAGAACTTTCCGGCATCCG
CGAAGAATAAGGCAATCAAA

Downstream 100 bases:

>100_bases
GCCATGCACCTGCTTCCTGAACTCGCCAGCCACCATGCGGTGTCAATTCCCGAGCTGCTCGTCAGCCGGGATGAAAGGCA
AGCACGGCAACACGTCTGGC

Product: citrate lyase, citrate-ACP transferase subunit alpha

Products: NA

Alternate protein names: Citrase alpha chain; Citrate (pro-3S)-lyase alpha chain; Citrate CoA-transferase subunit [H]

Number of amino acids: Translated: 510; Mature: 509

Protein sequence:

>510_residues
MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDRKLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNM
VMDVIAKMGFKNLTLASSSLSDCHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASIEQDQVDLIVKVDRVGDAAKIGA
GATRMTTNPRELLIARSAADVIFNSGYFKEGFSMQTGTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLI
RKLLDVQSFDSHAAQSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRGASGGHCDT
AIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPARPELAERLQEAGIKVVSIEWLRERARLLTGE
PQPIEFTDRVVAVVRYRDGSVIDVVHQVKE

Sequences:

>Translated_510_residues
MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDRKLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNM
VMDVIAKMGFKNLTLASSSLSDCHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASIEQDQVDLIVKVDRVGDAAKIGA
GATRMTTNPRELLIARSAADVIFNSGYFKEGFSMQTGTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLI
RKLLDVQSFDSHAAQSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRGASGGHCDT
AIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPARPELAERLQEAGIKVVSIEWLRERARLLTGE
PQPIEFTDRVVAVVRYRDGSVIDVVHQVKE
>Mature_509_residues
TQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDRKLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMV
MDVIAKMGFKNLTLASSSLSDCHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNID
VAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASIEQDQVDLIVKVDRVGDAAKIGAG
ATRMTTNPRELLIARSAADVIFNSGYFKEGFSMQTGTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIR
KLLDVQSFDSHAAQSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRGASGGHCDTA
IASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPARPELAERLQEAGIKVVSIEWLRERARLLTGEP
QPIEFTDRVVAVVRYRDGSVIDVVHQVKE

Specific function: Represents a citrate:acetyl-ACP transferase [H]

COG id: COG3051

COG function: function code C; Citrate lyase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786832, Length=510, Percent_Identity=99.6078431372549, Blast_Score=1045, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006472 [H]

Pfam domain/function: PF04223 CitF [H]

EC number: =4.1.3.6; =2.8.3.10 [H]

Molecular weight: Translated: 55240; Mature: 55108

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDRKLCANLEEAIRRSGLQ
CCCHHHHHHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSDCHAPLVEHIRQGVVTRIY
CCCEEEEEEEECCCCEEHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHH
TSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNIDVAFLGVPSCDEFGNANGYT
HHCCCCCHHHHHHCCHHCCCCEEECCCCEEEEEECCCEEEEEEEEECCCCHHCCCCCCCC
GKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASIEQDQVDLIVKVDRVGDAAKIGA
CHHHHHHCCEEEEECCCCCEEEEEEHHHCCCCCCCCCCCCCCEEEEEEEECCCCHHHCCC
GATRMTTNPRELLIARSAADVIFNSGYFKEGFSMQTGTGGASLAVTRFLEDKMRSRDIRA
CCCCCCCCHHHHEEEHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCC
DFALGGITATMVDLHEKGLIRKLLDVQSFDSHAAQSLARNPNHIEISANQYANWGSKGAS
CEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCC
VDRLDVVVLSALEIDTQFNVNVLTGSDGVLRGASGGHCDTAIASALSIIVAPLVRGRIPT
CCHHHHEEHHHHEECCEEEEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCHH
LVDNVLTCITPGSSVDILVTDHGIAVNPARPELAERLQEAGIKVVSIEWLRERARLLTGE
HHHHHHHHCCCCCCEEEEEECCCEEECCCCHHHHHHHHHCCCEEEEHHHHHHHHHHHCCC
PQPIEFTDRVVAVVRYRDGSVIDVVHQVKE
CCCCCHHCEEEEEEEECCCCHHHHHHHHCC
>Mature Secondary Structure 
TQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDRKLCANLEEAIRRSGLQ
CCHHHHHHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSDCHAPLVEHIRQGVVTRIY
CCCEEEEEEEECCCCEEHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHH
TSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNIDVAFLGVPSCDEFGNANGYT
HHCCCCCHHHHHHCCHHCCCCEEECCCCEEEEEECCCEEEEEEEEECCCCHHCCCCCCCC
GKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASIEQDQVDLIVKVDRVGDAAKIGA
CHHHHHHCCEEEEECCCCCEEEEEEHHHCCCCCCCCCCCCCCEEEEEEEECCCCHHHCCC
GATRMTTNPRELLIARSAADVIFNSGYFKEGFSMQTGTGGASLAVTRFLEDKMRSRDIRA
CCCCCCCCHHHHEEEHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCC
DFALGGITATMVDLHEKGLIRKLLDVQSFDSHAAQSLARNPNHIEISANQYANWGSKGAS
CEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCC
VDRLDVVVLSALEIDTQFNVNVLTGSDGVLRGASGGHCDTAIASALSIIVAPLVRGRIPT
CCHHHHEEHHHHEECCEEEEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCHH
LVDNVLTCITPGSSVDILVTDHGIAVNPARPELAERLQEAGIKVVSIEWLRERARLLTGE
HHHHHHHHCCCCCCEEEEEECCCEEECCCCHHHHHHHHHCCCEEEEHHHHHHHHHHHCCC
PQPIEFTDRVVAVVRYRDGSVIDVVHQVKE
CCCCCHHCEEEEEEEECCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]