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The map label for this gene is glpD

Identifier: 218702170

GI number: 218702170

Start: 4056679

End: 4058184

Strand: Direct

Name: glpD

Synonym: ECIAI39_3906

Alternate gene names: 218702170

Gene position: 4056679-4058184 (Clockwise)

Preceding gene: 218702166

Following gene: 218702179

Centisome position: 79.05

GC content: 55.11

Gene sequence:

>1506_bases
ATGGAAACCAAAGATCTGATTGTGATAGGTGGCGGCATCAATGGTGCTGGTATCGCGGCAGACGCCGCTGGACGCGGTTT
ATCCGTGCTGATGCTGGAGGCGCAGGATCTCGCTTGCGCGACCTCTTCCGCCAGTTCAAAACTCATTCACGGTGGCCTGC
GCTACCTTGAGCACTATGAATTCCGCCTGGTCAGCGAGGCGCTGGCTGAACGTGAAGTGTTGCTGAAAATGGCTCCGCAT
ATCGCCTTCCCGATGCGTTTTCGCCTGCCACATCGTCCGCATCTGCGCCCGGCGTGGATGATTCGCATTGGTCTGTTTAT
GTACGATCATCTGGGTAAACGCATCAGCCTGCCGGGATCAACCGGTTTGCGTTTTGGCGCAAACTCAGTGTTAAAACCGG
AAATTAAGCGCGGATTCGAATATTCTGACTGTTGGGTAGACGACGCCCGTCTGGTACTCGCCAACGCCCAGATGGTGGTA
CGTAAAGGCGGTGAAGTGCTTACTCGTACTCGTGCCACTTCTGCTCGCCGCGAAAACGGCCTGTGGATTGTGGAAGCAGA
AGATATCGATACCGGCAAAAAATACACCTGGCAGGCGCGCGGCCTGGTGAACGCCACCGGCCCGTGGGTGAAACAGTTCT
TCGACGAGGGGATGCATCTGCCTTCGCCTTATGGCATTCGCCTGATCAAAGGCAGCCATATTGTGGTGCCACGCGTGCAT
ACCCAGAAGCAAGCCTACATTCTGCAAAACGAAGATAAACGTATTGTGTTCGTGATCCCGTGGATGGACGAGTTTTCCAT
CATCGGCACCACCGATGTGGAATACAAAGGTGATCCGAAAGCCGTGAAGATTGAAGAGAGTGAAATCAATTACCTGCTGA
AAGTGTATAACACGCACTTTAAAAAGCAGTTAAGCCGTGACGATATCGTCTGGACTTACTCCGGCGTGCGTCCGCTGTGT
GATGATGAGTCCGACTCGCCGCAGGCAATTACCCGTGATTACACCCTTGATATCCATGACGAAAACGGCAAAGCACCGCT
GCTGTCGGTATTCGGCGGTAAGCTGACCACCTACCGCAAACTGGCGGAACATGCGCTGGAAAAACTGACGCCGTATTATC
AGAGTATTGGCCCGGCGTGGACGAAAGAGAGCGTGCTGCCGGGAGGTGCCATTGAAGGCGACCGCGACGATTACGCCGCT
CGCCTGCGCCGCCGTTACCCGTTCCTGACTGAATCGCTGGCACGTCACTACGCACGCACTTACGGCAGCAACAGCGAACT
GCTGCTCGGCAATGCGGGGGCGATAAGCGACCTCGGGGAGGATTTCGGTCATGAGTTCTACGAAGCGGAACTGAAATACC
TGGTGGATCACGAATGGGTCCGTCGCGCCGACGATGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGAT
CAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGTTGTCGCTGGCGTCGTAA

Upstream 100 bases:

>100_bases
ACTCTAAAATGTTTTTTCAATGTTACCCAAAGCGCAATTCTTTGCTAATATGTTCGATAACGAACATTTATGAACTTTAA
CGAAAGTGAATGAGGGCAGC

Downstream 100 bases:

>100_bases
ATTAACGTAAGGTGGCCAGGTCAGATTTCAATCTGACCTGAGACTGATGATAAACAAAAAAACTGCCTGATGCGCTACGC
TTATCAGGCCTACGTGGTTT

Product: glycerol-3-phosphate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 501; Mature: 501

Protein sequence:

>501_residues
METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSASSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPH
IAFPMRFRLPHRPHLRPAWMIRIGLFMYDHLGKRISLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYTWQARGLVNATGPWVKQFFDEGMHLPSPYGIRLIKGSHIVVPRVH
TQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYKGDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLC
DDESDSPQAITRDYTLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQSIGPAWTKESVLPGGAIEGDRDDYAA
RLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAELKYLVDHEWVRRADDALWRRTKQGMWLNAD
QQSRVSQWLVEYTQQKLSLAS

Sequences:

>Translated_501_residues
METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSASSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPH
IAFPMRFRLPHRPHLRPAWMIRIGLFMYDHLGKRISLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYTWQARGLVNATGPWVKQFFDEGMHLPSPYGIRLIKGSHIVVPRVH
TQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYKGDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLC
DDESDSPQAITRDYTLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQSIGPAWTKESVLPGGAIEGDRDDYAA
RLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAELKYLVDHEWVRRADDALWRRTKQGMWLNAD
QQSRVSQWLVEYTQQKLSLAS
>Mature_501_residues
METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSASSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPH
IAFPMRFRLPHRPHLRPAWMIRIGLFMYDHLGKRISLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYTWQARGLVNATGPWVKQFFDEGMHLPSPYGIRLIKGSHIVVPRVH
TQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYKGDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLC
DDESDSPQAITRDYTLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQSIGPAWTKESVLPGGAIEGDRDDYAA
RLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAELKYLVDHEWVRRADDALWRRTKQGMWLNAD
QQSRVSQWLVEYTQQKLSLAS

Specific function: Conversion of glycerol 3-phosphate to dihydroxyacetone. Uses molecular oxygen or nitrate as electron acceptor [H]

COG id: COG0578

COG function: function code C; Glycerol-3-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI285002233, Length=538, Percent_Identity=30.4832713754647, Blast_Score=176, Evalue=6e-44,
Organism=Homo sapiens, GI285002231, Length=538, Percent_Identity=30.4832713754647, Blast_Score=176, Evalue=6e-44,
Organism=Escherichia coli, GI2367226, Length=501, Percent_Identity=98.4031936127745, Blast_Score=1023, Evalue=0.0,
Organism=Escherichia coli, GI1788574, Length=385, Percent_Identity=26.2337662337662, Blast_Score=88, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI115534726, Length=514, Percent_Identity=30.3501945525292, Blast_Score=155, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI17509253, Length=542, Percent_Identity=29.3357933579336, Blast_Score=152, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6322036, Length=531, Percent_Identity=28.2485875706215, Blast_Score=161, Evalue=2e-40,
Organism=Drosophila melanogaster, GI161077125, Length=511, Percent_Identity=30.7240704500978, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24653942, Length=511, Percent_Identity=30.7240704500978, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24653944, Length=511, Percent_Identity=30.7240704500978, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI20130025, Length=511, Percent_Identity=30.7240704500978, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24586295, Length=448, Percent_Identity=27.6785714285714, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI19921278, Length=496, Percent_Identity=23.3870967741935, Blast_Score=88, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076
- InterPro:   IPR000447 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: =1.1.5.3 [H]

Molecular weight: Translated: 56791; Mature: 56791

Theoretical pI: Translated: 7.79; Mature: 7.79

Prosite motif: PS00977 FAD_G3PDH_1 ; PS00978 FAD_G3PDH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSASSKLIHGGLRYLEHYE
CCCCCEEEECCCCCCCCEECCCCCCCEEEEEEECCHHHEECCCCCHHHHHHHHHHHHHHH
FRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIRIGLFMYDHLGKRISLPGS
HHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCEEECCCC
TGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVVRKGGEVLTRTRATSARRENG
CCCCCCCCCCCCHHHHCCCCCCCCEECCCEEEEECCHHHHHCCCHHEEHHHHHHHHHCCC
LWIVEAEDIDTGKKYTWQARGLVNATGPWVKQFFDEGMHLPSPYGIRLIKGSHIVVPRVH
EEEEECCCCCCCCCEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCEEEEECCEEEEECCC
TQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYKGDPKAVKIEESEINYLLKVYNTHF
CCCEEEEEECCCCEEEEEEECCCCCEEEECCCCCCCCCCCEEEEEHHHHHHHHHHHHHHH
KKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDYTLDIHDENGKAPLLSVFGGKLTTYRK
HHHCCCCCEEEEECCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCCCHHHCCCCHHHHHH
LAEHALEKLTPYYQSIGPAWTKESVLPGGAIEGDRDDYAARLRRRYPFLTESLARHYART
HHHHHHHHHCHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHH
YGSNSELLLGNAGAISDLGEDFGHEFYEAELKYLVDHEWVRRADDALWRRTKQGMWLNAD
CCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCC
QQSRVSQWLVEYTQQKLSLAS
HHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSASSKLIHGGLRYLEHYE
CCCCCEEEECCCCCCCCEECCCCCCCEEEEEEECCHHHEECCCCCHHHHHHHHHHHHHHH
FRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIRIGLFMYDHLGKRISLPGS
HHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCEEECCCC
TGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVVRKGGEVLTRTRATSARRENG
CCCCCCCCCCCCHHHHCCCCCCCCEECCCEEEEECCHHHHHCCCHHEEHHHHHHHHHCCC
LWIVEAEDIDTGKKYTWQARGLVNATGPWVKQFFDEGMHLPSPYGIRLIKGSHIVVPRVH
EEEEECCCCCCCCCEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCEEEEECCEEEEECCC
TQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYKGDPKAVKIEESEINYLLKVYNTHF
CCCEEEEEECCCCEEEEEEECCCCCEEEECCCCCCCCCCCEEEEEHHHHHHHHHHHHHHH
KKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDYTLDIHDENGKAPLLSVFGGKLTTYRK
HHHCCCCCEEEEECCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCCCHHHCCCCHHHHHH
LAEHALEKLTPYYQSIGPAWTKESVLPGGAIEGDRDDYAARLRRRYPFLTESLARHYART
HHHHHHHHHCHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHH
YGSNSELLLGNAGAISDLGEDFGHEFYEAELKYLVDHEWVRRADDALWRRTKQGMWLNAD
CCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCC
QQSRVSQWLVEYTQQKLSLAS
HHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1987111; 9278503; 3045087; 3045764 [H]