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The map label for this gene is rtcR

Identifier: 218702166

GI number: 218702166

Start: 4052933

End: 4054531

Strand: Direct

Name: rtcR

Synonym: ECIAI39_3902

Alternate gene names: 218702166

Gene position: 4052933-4054531 (Clockwise)

Preceding gene: 218702163

Following gene: 218702170

Centisome position: 78.97

GC content: 55.1

Gene sequence:

>1599_bases
ATGCGTAAAACAGTGGCTTTTGGCTTTGTCGGCACCGTACTGGATTATGCCGGGCGCGGCAGTCAGCGATGGTCGAAATG
GCGTCCGTCACTCTGTCTATGCCAGCAAGAATCGTTAGTCATCAATCGACTGGAATTGTTGCACGACGCCCGCTCACGCT
CGCTGTTTGAAACGCTTAAACGCGATATCGCCAATGTTTCGCCAGAAACAAAAGTGGTGGGCGTTGAGATAGAACTGCAT
AACCCGTGGGATTTCGAAGAGGTTTACGCCTGCCTGCATGATTTCGCCCGTGGTTACGCGTTCCAGCCAGAAAAAGAAGA
CTATTTAATTCACATCACCACCGGTACCCACGTCGCGCAGATTTGCTGGTTTCTGCTGGCAGAAGCGCGTTATCTACCCG
CCCGGTTGATACAATCTTCACCACCGCGCAAAAAAGAACAACCGCGCGGCGCAGGTGAAGTGACGATTATCGATCTCGAT
TTAAGCCGTTATAACGCCATCGCCAGCCGCTTTGCCGAGGAACGCCAGCAAACCCTCGATTTCCTCAAATCCGGCATTGC
CACGCGTAACGCCCACTTCAACCGCATGATTGAGCAGATCGAAAAAGTGGCGATCAAATCCCGCGCACCGATCCTGCTCA
ACGGCCCAACCGGTGCGGGAAAGTCGTTTCTGGCACGACGCATCTTCGAGTTAAAACAGGCGCGGCATCAGTTTAGCGGC
GCGTTTGTGGAGGTGAACTGTGCCACTCTGCGCGGCGATACCGCCATGTCAGCGCTGTTTGGTCATGTGAAAGGCGCGTT
TACCGGGGCGCGGGAGTCGCGGGAAGGATTATTACGCAGCGCCAACGGCGGGATGTTATTTCTTGATGAGATTGGCGAAC
TGGGCGCAGACGAACAGGCAATGCTGCTGAAAGCCATTGAAGAGAAAACCTTTTACCCGTTTGGCAGCGATCGCCAGGTG
AGTAGCGATTTTCAGCTTATCGCCGGAACGGTGCGCGATTTGCGCCAACTGGTTGTCGAAGGCAAATTTCGCGAAGACCT
GTACGCACGGATTAATCTCTGGACTTTCGCTCTGCCGGGGCTACGCCAGCGCCAGGAAGATATTGAACCTAACCTGGATT
ATGAAGTGGAGCGCCACGCCTCACTTACCGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCCTTT
GCAACATCATCTCAGGCGGCATGGCGCGGTAACTTTCGCGAGCTTTCTGCCAGCGTCACGCGAATGGCAACCTTTGCCAC
TAGCGGACGCATCACTCTGGAAGTGGTTGAAGATGAGATAAACCGTCTGCGCTATAACTGGCAGGAGAGCCGCCCTTCCG
TGCTTACACAGCTACTGGGCGCTGAAGCGGAAAACATCGACCTCTTTGATCGACTGCAACTGGAACACGTTATCGCTATC
TGCCGTCAGGCAAAGTCGCTTTCCGCTGCCGGACGCCAGCTTTTTGACGTTTCGCGCCAAGGGAAAGCCAGCGTCAACGA
CGCGGATCGGCTACGCAAATACCTGGCGCGTTTTAATCTGACGTGGGAAGCCGTGCAGGATCAGCACAGCTCCAGTTGA

Upstream 100 bases:

>100_bases
TTGTTTTTAAATAGATAAAATCTTTACGTCCGTAAACGGAGATTTCCCGCAAAGCCAATTCGCCGATAATGAAATATCGT
CTTTTATAAGGATATCTAAG

Downstream 100 bases:

>100_bases
ATATGGTGGTCCGTCAGCACCTGCATCACGCTTACTGGCGGCGGGGCGTCGGTGTAGACGGCATCTACCATGCTGATACT
GCCCATATTGACCATCGCGT

Product: sigma 54-dependent transcriptional regulator of rtcBA expression

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 532; Mature: 532

Protein sequence:

>532_residues
MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCLCQQESLVINRLELLHDARSRSLFETLKRDIANVSPETKVVGVEIELH
NPWDFEEVYACLHDFARGYAFQPEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
LSRYNAIASRFAEERQQTLDFLKSGIATRNAHFNRMIEQIEKVAIKSRAPILLNGPTGAGKSFLARRIFELKQARHQFSG
AFVEVNCATLRGDTAMSALFGHVKGAFTGARESREGLLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQV
SSDFQLIAGTVRDLRQLVVEGKFREDLYARINLWTFALPGLRQRQEDIEPNLDYEVERHASLTGDSVRFNTEARRAWLAF
ATSSQAAWRGNFRELSASVTRMATFATSGRITLEVVEDEINRLRYNWQESRPSVLTQLLGAEAENIDLFDRLQLEHVIAI
CRQAKSLSAAGRQLFDVSRQGKASVNDADRLRKYLARFNLTWEAVQDQHSSS

Sequences:

>Translated_532_residues
MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCLCQQESLVINRLELLHDARSRSLFETLKRDIANVSPETKVVGVEIELH
NPWDFEEVYACLHDFARGYAFQPEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
LSRYNAIASRFAEERQQTLDFLKSGIATRNAHFNRMIEQIEKVAIKSRAPILLNGPTGAGKSFLARRIFELKQARHQFSG
AFVEVNCATLRGDTAMSALFGHVKGAFTGARESREGLLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQV
SSDFQLIAGTVRDLRQLVVEGKFREDLYARINLWTFALPGLRQRQEDIEPNLDYEVERHASLTGDSVRFNTEARRAWLAF
ATSSQAAWRGNFRELSASVTRMATFATSGRITLEVVEDEINRLRYNWQESRPSVLTQLLGAEAENIDLFDRLQLEHVIAI
CRQAKSLSAAGRQLFDVSRQGKASVNDADRLRKYLARFNLTWEAVQDQHSSS
>Mature_532_residues
MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCLCQQESLVINRLELLHDARSRSLFETLKRDIANVSPETKVVGVEIELH
NPWDFEEVYACLHDFARGYAFQPEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
LSRYNAIASRFAEERQQTLDFLKSGIATRNAHFNRMIEQIEKVAIKSRAPILLNGPTGAGKSFLARRIFELKQARHQFSG
AFVEVNCATLRGDTAMSALFGHVKGAFTGARESREGLLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQV
SSDFQLIAGTVRDLRQLVVEGKFREDLYARINLWTFALPGLRQRQEDIEPNLDYEVERHASLTGDSVRFNTEARRAWLAF
ATSSQAAWRGNFRELSASVTRMATFATSGRITLEVVEDEINRLRYNWQESRPSVLTQLLGAEAENIDLFDRLQLEHVIAI
CRQAKSLSAAGRQLFDVSRQGKASVNDADRLRKYLARFNLTWEAVQDQHSSS

Specific function: Transcriptional repressor of the rtcAB genes. Interacts with sigma-54 [H]

COG id: COG4650

COG function: function code KT; Sigma54-dependent transcription regulator containing an AAA-type ATPase domain and a DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1789828, Length=532, Percent_Identity=96.4285714285714, Blast_Score=1059, Evalue=0.0,
Organism=Escherichia coli, GI1788905, Length=275, Percent_Identity=35.2727272727273, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1788550, Length=252, Percent_Identity=37.6984126984127, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1789233, Length=233, Percent_Identity=37.3390557939914, Blast_Score=149, Evalue=6e-37,
Organism=Escherichia coli, GI1790437, Length=249, Percent_Identity=38.1526104417671, Blast_Score=148, Evalue=9e-37,
Organism=Escherichia coli, GI87082117, Length=243, Percent_Identity=35.8024691358025, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI1789087, Length=254, Percent_Identity=36.2204724409449, Blast_Score=142, Evalue=6e-35,
Organism=Escherichia coli, GI1787583, Length=244, Percent_Identity=35.655737704918, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI87082152, Length=244, Percent_Identity=36.4754098360656, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI1790299, Length=237, Percent_Identity=35.0210970464135, Blast_Score=132, Evalue=6e-32,
Organism=Escherichia coli, GI1786524, Length=260, Percent_Identity=35.3846153846154, Blast_Score=125, Evalue=9e-30,
Organism=Escherichia coli, GI87081872, Length=232, Percent_Identity=34.051724137931, Blast_Score=111, Evalue=9e-26,
Organism=Escherichia coli, GI87081858, Length=243, Percent_Identity=30.0411522633745, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR002078
- InterPro:   IPR009715
- InterPro:   IPR017183 [H]

Pfam domain/function: PF06956 RtcR; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 60271; Mature: 60271

Theoretical pI: Translated: 8.14; Mature: 8.14

Prosite motif: PS00676 SIGMA54_INTERACT_2 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCLCQQESLVINRLELLHDARSRSLFETLK
CCCEEEHHHHHHHHHHCCCCCCHHHHCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHH
RDIANVSPETKVVGVEIELHNPWDFEEVYACLHDFARGYAFQPEKEDYLIHITTGTHVAQ
HHHHCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCHHHHH
ICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLDLSRYNAIASRFAEERQQTLD
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHH
FLKSGIATRNAHFNRMIEQIEKVAIKSRAPILLNGPTGAGKSFLARRIFELKQARHQFSG
HHHHCHHHCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCC
AFVEVNCATLRGDTAMSALFGHVKGAFTGARESREGLLRSANGGMLFLDEIGELGADEQA
CEEEEEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEEEHHHCCCCCHHH
MLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDLRQLVVEGKFREDLYARINLWTFALPG
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEECCC
LRQRQEDIEPNLDYEVERHASLTGDSVRFNTEARRAWLAFATSSQAAWRGNFRELSASVT
HHHHHHCCCCCCCCCHHHCCCCCCCCEEECHHHHHEEEEEECCCCHHHCCCHHHHHHHHH
RMATFATSGRITLEVVEDEINRLRYNWQESRPSVLTQLLGAEAENIDLFDRLQLEHVIAI
HHHHHCCCCCEEEEEHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
CRQAKSLSAAGRQLFDVSRQGKASVNDADRLRKYLARFNLTWEAVQDQHSSS
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHCCCCC
>Mature Secondary Structure
MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCLCQQESLVINRLELLHDARSRSLFETLK
CCCEEEHHHHHHHHHHCCCCCCHHHHCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHH
RDIANVSPETKVVGVEIELHNPWDFEEVYACLHDFARGYAFQPEKEDYLIHITTGTHVAQ
HHHHCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCHHHHH
ICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLDLSRYNAIASRFAEERQQTLD
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHH
FLKSGIATRNAHFNRMIEQIEKVAIKSRAPILLNGPTGAGKSFLARRIFELKQARHQFSG
HHHHCHHHCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCC
AFVEVNCATLRGDTAMSALFGHVKGAFTGARESREGLLRSANGGMLFLDEIGELGADEQA
CEEEEEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEEEHHHCCCCCHHH
MLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDLRQLVVEGKFREDLYARINLWTFALPG
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEECCC
LRQRQEDIEPNLDYEVERHASLTGDSVRFNTEARRAWLAFATSSQAAWRGNFRELSASVT
HHHHHHCCCCCCCCCHHHCCCCCCCCEEECHHHHHEEEEEECCCCHHHCCCHHHHHHHHH
RMATFATSGRITLEVVEDEINRLRYNWQESRPSVLTQLLGAEAENIDLFDRLQLEHVIAI
HHHHHCCCCCEEEEEHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
CRQAKSLSAAGRQLFDVSRQGKASVNDADRLRKYLARFNLTWEAVQDQHSSS
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 3045764; 7984428; 7920643; 9738023 [H]