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The map label for this gene is kpsD

Identifier: 218701716

GI number: 218701716

Start: 3585440

End: 3587119

Strand: Direct

Name: kpsD

Synonym: ECIAI39_3435

Alternate gene names: 218701716

Gene position: 3585440-3587119 (Clockwise)

Preceding gene: 218701715

Following gene: 218701717

Centisome position: 69.86

GC content: 52.02

Gene sequence:

>1680_bases
GTGATGAAATTATTTAAATCAATTTTACTGATTGCCGCCTGTCACGCGGCGCAGGCCAGCGCGGCCATTGATATTAACGC
TGACCCAAACCTGACAGGAGCCGCGCCGCTTACCGGTATTCTGAACGGGCAACAGTCGGATACGCAAAACATGAGCGGCT
TCGACAATACCCCACCGCCCGCACCGCCGGTGGTAATGAGCCGTATGTTTGGTGCTCAACTTTTCAACGGCACCAGCGCG
GATAGCGGTGCGACGGTAGGATTCAACCCTGACTATATTCTGAATCCGGGTGATAGCATTCAGGTTCGCTTGTGGGGTGC
GTTCACCTTTGATGGTGCGTTACAGGTTGATCCCAAAGGTAATATTTTCCTGCCGAACGTTGGTCCGGTGAAAGTTGCTG
GCGTCAGTAATAGTCAGCTAAATGCCCTGGTCACATCCAAAGTGAAGGAAGTATACCAGTCCAACGTCAACGTCTACGCC
TCCTTATTACAGGCGCAGCCAGTAAAAGTGTACGTGACCGGATTTGTGCGTAATCCTGGTCTGTATGGCGGCGTGACGTC
TGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGATCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGC
GCGGTAACCGCGTGCGCTCCAACGTCAACCTGTACGACTTCCTGCTGAACGGCAAACTGGGGCTTTCGCAGTTCGCCGAT
GGTGACACCATCATCGTCGGGCCGCGTCAGCATACTTTCAGCGTTCAGGGCGATGTCTTTAACAGCTACGACTTTGAGTT
CCGCGAAAGCAGCATTCCCGTAACGGAAGCGTTGAGCTGGGCGCGCCCTAAGCCTGGCGCGACTCACATTACGATTATGC
GTAAACAGGGGCTGCAAAAACGCAGCGAATACTATCCGATCAGTTCTGCGCCAGGCCGTATGTTGCAAAATGGCGATACC
TTAATCGTGAGCACTGACCGCTATGCCGGCACCATTCAGGTGCGGGTTGAAGGCGCACACTCCGGTGAACATGCCATGGT
ACTGCCTTATGGTTCCACTATGCGTGCGGTTCTGGAAAAAGTCCGCCCGAACAGCATGTCGCAGATGAACGCAGTTCAGC
TTTATCGCCCATCAGTGGCTCAGCGTCAGAAAGAGATGCTGAATCTCTCGCTGCAAAAGCTGGAGGAAGCATCACTTTCT
GCCCAGTCATCCACCAAAGAAGAAGCCAGCCTGCGAATGCAGGAAGCACAACTGATCAGCCGCTTTGTGGCGAAAGCACG
CACCGTTGTTCCGAAAGGTGAAGTGATCCTCAACGAATCCAATATTGATTCTGTTCTGCTTGAAGATGGCGACGTCATCA
ATATTCCGGAGAAAACATCGCTGGTTATGGTTCATGGCGAAGTGCTGTTCCCGAACGCGGTGAGCTGGCAGAAGGGTATG
ACCACCGAGGATTACATCGAGAAATGTGGTGGCCTGACGCAGAAATCGGGTAACGCCAGAATTATCGTCATTCGTCAGAA
CGGTGCAGCCGTCAACGCAGAAGATGTGGATTCACTCAAACCGGGCGATGAGATTATGGTTCTGCCGAAATATGAATCGA
AAAACATTGAAGTTACTCGCGGTATTTCCACCATCCTTTACCAGTTGGCGGTGGGTGCAAAAGTGATTCTGTCTTTGTAA

Upstream 100 bases:

>100_bases
CCTGCTGGTTACTGGTGTGCTGCCTGCTGTTCGGCACCCTGAAACTGTTGCTGGCTGTTATTGAAGATCACCGAGACTAA
CGCTGTCGCTGAATGAGTTT

Downstream 100 bases:

>100_bases
GGAGTTGAAATGAGCAAAGCAGTTATTGTCATTCCGGCTCGCTATGGCTCCTCGCGCCTGCCGGGTAAGCCACTGCTCGA
TATTGTTGGTAAACCGATGA

Product: polysialic acid transport protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 559; Mature: 559

Protein sequence:

>559_residues
MMKLFKSILLIAACHAAQASAAIDINADPNLTGAAPLTGILNGQQSDTQNMSGFDNTPPPAPPVVMSRMFGAQLFNGTSA
DSGATVGFNPDYILNPGDSIQVRLWGAFTFDGALQVDPKGNIFLPNVGPVKVAGVSNSQLNALVTSKVKEVYQSNVNVYA
SLLQAQPVKVYVTGFVRNPGLYGGVTSDSLLNYLIKAGGVDPERGSYVDIVVKRGNRVRSNVNLYDFLLNGKLGLSQFAD
GDTIIVGPRQHTFSVQGDVFNSYDFEFRESSIPVTEALSWARPKPGATHITIMRKQGLQKRSEYYPISSAPGRMLQNGDT
LIVSTDRYAGTIQVRVEGAHSGEHAMVLPYGSTMRAVLEKVRPNSMSQMNAVQLYRPSVAQRQKEMLNLSLQKLEEASLS
AQSSTKEEASLRMQEAQLISRFVAKARTVVPKGEVILNESNIDSVLLEDGDVINIPEKTSLVMVHGEVLFPNAVSWQKGM
TTEDYIEKCGGLTQKSGNARIIVIRQNGAAVNAEDVDSLKPGDEIMVLPKYESKNIEVTRGISTILYQLAVGAKVILSL

Sequences:

>Translated_559_residues
MMKLFKSILLIAACHAAQASAAIDINADPNLTGAAPLTGILNGQQSDTQNMSGFDNTPPPAPPVVMSRMFGAQLFNGTSA
DSGATVGFNPDYILNPGDSIQVRLWGAFTFDGALQVDPKGNIFLPNVGPVKVAGVSNSQLNALVTSKVKEVYQSNVNVYA
SLLQAQPVKVYVTGFVRNPGLYGGVTSDSLLNYLIKAGGVDPERGSYVDIVVKRGNRVRSNVNLYDFLLNGKLGLSQFAD
GDTIIVGPRQHTFSVQGDVFNSYDFEFRESSIPVTEALSWARPKPGATHITIMRKQGLQKRSEYYPISSAPGRMLQNGDT
LIVSTDRYAGTIQVRVEGAHSGEHAMVLPYGSTMRAVLEKVRPNSMSQMNAVQLYRPSVAQRQKEMLNLSLQKLEEASLS
AQSSTKEEASLRMQEAQLISRFVAKARTVVPKGEVILNESNIDSVLLEDGDVINIPEKTSLVMVHGEVLFPNAVSWQKGM
TTEDYIEKCGGLTQKSGNARIIVIRQNGAAVNAEDVDSLKPGDEIMVLPKYESKNIEVTRGISTILYQLAVGAKVILSL
>Mature_559_residues
MMKLFKSILLIAACHAAQASAAIDINADPNLTGAAPLTGILNGQQSDTQNMSGFDNTPPPAPPVVMSRMFGAQLFNGTSA
DSGATVGFNPDYILNPGDSIQVRLWGAFTFDGALQVDPKGNIFLPNVGPVKVAGVSNSQLNALVTSKVKEVYQSNVNVYA
SLLQAQPVKVYVTGFVRNPGLYGGVTSDSLLNYLIKAGGVDPERGSYVDIVVKRGNRVRSNVNLYDFLLNGKLGLSQFAD
GDTIIVGPRQHTFSVQGDVFNSYDFEFRESSIPVTEALSWARPKPGATHITIMRKQGLQKRSEYYPISSAPGRMLQNGDT
LIVSTDRYAGTIQVRVEGAHSGEHAMVLPYGSTMRAVLEKVRPNSMSQMNAVQLYRPSVAQRQKEMLNLSLQKLEEASLS
AQSSTKEEASLRMQEAQLISRFVAKARTVVPKGEVILNESNIDSVLLEDGDVINIPEKTSLVMVHGEVLFPNAVSWQKGM
TTEDYIEKCGGLTQKSGNARIIVIRQNGAAVNAEDVDSLKPGDEIMVLPKYESKNIEVTRGISTILYQLAVGAKVILSL

Specific function: Involved in the translocation of the polysialic acid capsule across the outer membrane to the cell surface. May function as the periplasmic binding element of the PSA transport system, in which it transiently interacts with the membrane component of the t

COG id: COG1596

COG function: function code M; Periplasmic protein involved in polysaccharide export

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To E.coli K5 kpsD [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003715 [H]

Pfam domain/function: PF02563 Poly_export [H]

EC number: NA

Molecular weight: Translated: 60487; Mature: 60487

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKLFKSILLIAACHAAQASAAIDINADPNLTGAAPLTGILNGQQSDTQNMSGFDNTPPP
CHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCC
APPVVMSRMFGAQLFNGTSADSGATVGFNPDYILNPGDSIQVRLWGAFTFDGALQVDPKG
CCHHHHHHHHHHHHCCCCCCCCCCEECCCCCEEECCCCEEEEEEEEEEEECCEEEECCCC
NIFLPNVGPVKVAGVSNSQLNALVTSKVKEVYQSNVNVYASLLQAQPVKVYVTGFVRNPG
CEECCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEEEEEEECCC
LYGGVTSDSLLNYLIKAGGVDPERGSYVDIVVKRGNRVRSNVNLYDFLLNGKLGLSQFAD
CCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCEEEEEECCCCCHHHCCC
GDTIIVGPRQHTFSVQGDVFNSYDFEFRESSIPVTEALSWARPKPGATHITIMRKQGLQK
CCEEEECCCCEEEEECCCCCCCCCCEECCCCCCHHHHHHHCCCCCCCEEEEEEEHHCCHH
RSEYYPISSAPGRMLQNGDTLIVSTDRYAGTIQVRVEGAHSGEHAMVLPYGSTMRAVLEK
HHHCCCCCCCCCHHHCCCCEEEEECCCCCEEEEEEEECCCCCCEEEEEECCHHHHHHHHH
VRPNSMSQMNAVQLYRPSVAQRQKEMLNLSLQKLEEASLSAQSSTKEEASLRMQEAQLIS
HCCCCHHHHCHHEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
RFVAKARTVVPKGEVILNESNIDSVLLEDGDVINIPEKTSLVMVHGEVLFPNAVSWQKGM
HHHHHHHHCCCCCCEEEECCCCCEEEECCCCEEECCCCCEEEEEECCEECCCCCCCCCCC
TTEDYIEKCGGLTQKSGNARIIVIRQNGAAVNAEDVDSLKPGDEIMVLPKYESKNIEVTR
CHHHHHHHHCCCCCCCCCEEEEEEECCCCEECHHHHHCCCCCCCEEEEECCCCCCEEEEH
GISTILYQLAVGAKVILSL
HHHHHHHHHHHCCEEEEEC
>Mature Secondary Structure
MMKLFKSILLIAACHAAQASAAIDINADPNLTGAAPLTGILNGQQSDTQNMSGFDNTPPP
CHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCC
APPVVMSRMFGAQLFNGTSADSGATVGFNPDYILNPGDSIQVRLWGAFTFDGALQVDPKG
CCHHHHHHHHHHHHCCCCCCCCCCEECCCCCEEECCCCEEEEEEEEEEEECCEEEECCCC
NIFLPNVGPVKVAGVSNSQLNALVTSKVKEVYQSNVNVYASLLQAQPVKVYVTGFVRNPG
CEECCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEEEEEEECCC
LYGGVTSDSLLNYLIKAGGVDPERGSYVDIVVKRGNRVRSNVNLYDFLLNGKLGLSQFAD
CCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCEEEEEECCCCCHHHCCC
GDTIIVGPRQHTFSVQGDVFNSYDFEFRESSIPVTEALSWARPKPGATHITIMRKQGLQK
CCEEEECCCCEEEEECCCCCCCCCCEECCCCCCHHHHHHHCCCCCCCEEEEEEEHHCCHH
RSEYYPISSAPGRMLQNGDTLIVSTDRYAGTIQVRVEGAHSGEHAMVLPYGSTMRAVLEK
HHHCCCCCCCCCHHHCCCCEEEEECCCCCEEEEEEEECCCCCCEEEEEECCHHHHHHHHH
VRPNSMSQMNAVQLYRPSVAQRQKEMLNLSLQKLEEASLSAQSSTKEEASLRMQEAQLIS
HCCCCHHHHCHHEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
RFVAKARTVVPKGEVILNESNIDSVLLEDGDVINIPEKTSLVMVHGEVLFPNAVSWQKGM
HHHHHHHHCCCCCCEEEECCCCCEEEECCCCEEECCCCCEEEEEECCEECCCCCCCCCCC
TTEDYIEKCGGLTQKSGNARIIVIRQNGAAVNAEDVDSLKPGDEIMVLPKYESKNIEVTR
CHHHHHHHHCCCCCCCCCEEEEEEECCCCEECHHHHHCCCCCCCEEEEECCCCCCEEEEH
GISTILYQLAVGAKVILSL
HHHHHHHHHHHCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8021185 [H]