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The map label for this gene is glnG

Identifier: 218701433

GI number: 218701433

Start: 3265021

End: 3266430

Strand: Direct

Name: glnG

Synonym: ECIAI39_3133

Alternate gene names: 218701433

Gene position: 3265021-3266430 (Clockwise)

Preceding gene: 218701432

Following gene: 218701436

Centisome position: 63.62

GC content: 56.6

Gene sequence:

>1410_bases
ATGCAACGAGGGATAGTCTGGGTAGTCGATGACGATAGTTCCATCCGTTGGGTGCTTGAACGTGCGCTCGCTGGAGCGGG
TTTAACCTGTACGACATTTGAGAACGGCGCGGAAGTACTGGAGGCGCTGGCGAGCAAAACGCCGGATGTGCTGCTTTCAG
ATATCCGTATGCCGGGAATGGACGGGCTGGCGCTGCTCAAGCAGATTAAACAGCGCCATCCAATGCTTCCGGTCATCATT
ATGACCGCACATTCCGATCTGGATGCTGCCGTCAGCGCCTATCAACAAGGGGCGTTTGATTATCTGCCCAAACCGTTTGA
TATCGACGAAGCCGTGGCGCTGGTTGAGCGCGCCATCAGCCATTATCAGGAACAGCAGCAGCCGCGTAATGTTCAGCTTA
ACGGCCCAACGACCGATATCATCGGCGAAGCGCCAGCCATGCAGGACGTGTTCCGTATTATCGGCCGGCTTTCGCGTTCT
TCTATTAGCGTGCTGATTAACGGCGAATCCGGCACCGGTAAAGAACTGGTCGCTCATGCCCTGCATCGCCACAGTCCGCG
AGCCAAAGCGCCATTTATCGCGCTGAATATGGCTGCTATCCCGAAGGATTTGATCGAATCAGAACTGTTTGGTCACGAGA
AAGGCGCATTTACCGGCGCGAATACCATTCGTCAGGGGCGTTTTGAACAGGCTGATGGCGGTACATTATTCCTCGATGAA
ATTGGCGATATGCCGCTGGATGTGCAGACGCGTTTGCTGCGCGTGCTGGCAGACGGTCAGTTTTATCGCGTTGGCGGCTA
TGCGCCGGTGAAAGTGGATGTGCGGATTATCGCTGCCACTCACCAGAACCTGGAACTGCGCGTGCAGGAAGGCAAGTTCC
GCGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTCATCTGCCGCCACTGCGTGAGCGTCGGGAAGATATTCCCCGT
CTGGCACGCCATTTTTTACAGGTTGCCGCGCGAGAGCTGGGCGTAGAAGCGAAGTTGCTGCATCCGGAAACCGAAGCCGC
GCTGACGCGTCTGGCATGGCCGGGTAACGTACGCCAGCTGGAAAACACCTGTCGCTGGCTAACGGTGATGGCTGCCGGGC
AGGAAGTGTTGATCCAGGATTTGCCTGGCGAACTGTTTGAATCAACGGTTGCGGAGAGTACTTCGCAAATGCAACCGGAC
AGTTGGGCGACACTTTTAGCGCAGTGGGCAGACAGAGCGCTGCGTTCCGGTCATCAAAATCTGCTTTCCGAAGCGCAGCC
TGAGCTGGAGCGGACGTTACTGACGACCGCGTTGCGACATACGCAAGGGCATAAACAGGAAGCGGCGCGGCTACTCGGCT
GGGGGCGTAACACTCTGACGCGGAAGTTAAAAGAGCTGGGGATGGAGTGA

Upstream 100 bases:

>100_bases
ATTTGATTGATCAGCATTCAGGCAAAATTGAATTTACCAGTTGGCCAGGTCATACCGAGTTCTCGGTTTACCTGCCTATC
AGGAAATAAAGGTGACGTTT

Downstream 100 bases:

>100_bases
TTCACGGCTTGTGTGTAAAGATTGATTATTGAGCGCAAATTGCTGGTATTTTGCGCTTTACTGTTCCGATAAGTTCAGTA
TGATCTTGCCCGGAAAACGG

Product: nitrogen regulation protein NR(I)

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALASKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVII
MTAHSDLDAAVSAYQQGAFDYLPKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFTGANTIRQGRFEQADGGTLFLDE
IGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRIIAATHQNLELRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPR
LARHFLQVAARELGVEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAESTSQMQPD
SWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAARLLGWGRNTLTRKLKELGME

Sequences:

>Translated_469_residues
MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALASKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVII
MTAHSDLDAAVSAYQQGAFDYLPKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFTGANTIRQGRFEQADGGTLFLDE
IGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRIIAATHQNLELRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPR
LARHFLQVAARELGVEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAESTSQMQPD
SWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAARLLGWGRNTLTRKLKELGME
>Mature_469_residues
MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALASKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVII
MTAHSDLDAAVSAYQQGAFDYLPKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFTGANTIRQGRFEQADGGTLFLDE
IGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRIIAATHQNLELRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPR
LARHFLQVAARELGVEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAESTSQMQPD
SWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAARLLGWGRNTLTRKLKELGME

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=469, Percent_Identity=99.7867803837953, Blast_Score=952, Evalue=0.0,
Organism=Escherichia coli, GI1788550, Length=468, Percent_Identity=42.9487179487179, Blast_Score=378, Evalue=1e-106,
Organism=Escherichia coli, GI1790437, Length=462, Percent_Identity=41.991341991342, Blast_Score=343, Evalue=1e-95,
Organism=Escherichia coli, GI1788905, Length=464, Percent_Identity=38.1465517241379, Blast_Score=295, Evalue=3e-81,
Organism=Escherichia coli, GI87082117, Length=364, Percent_Identity=42.032967032967, Blast_Score=254, Evalue=1e-68,
Organism=Escherichia coli, GI1789233, Length=329, Percent_Identity=43.161094224924, Blast_Score=244, Evalue=6e-66,
Organism=Escherichia coli, GI1789087, Length=367, Percent_Identity=40.3269754768392, Blast_Score=235, Evalue=4e-63,
Organism=Escherichia coli, GI1786524, Length=345, Percent_Identity=43.4782608695652, Blast_Score=235, Evalue=6e-63,
Organism=Escherichia coli, GI1787583, Length=331, Percent_Identity=40.4833836858006, Blast_Score=218, Evalue=6e-58,
Organism=Escherichia coli, GI87082152, Length=336, Percent_Identity=40.4761904761905, Blast_Score=217, Evalue=1e-57,
Organism=Escherichia coli, GI87081872, Length=331, Percent_Identity=39.2749244712991, Blast_Score=202, Evalue=5e-53,
Organism=Escherichia coli, GI87081858, Length=312, Percent_Identity=32.0512820512821, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1789828, Length=239, Percent_Identity=35.1464435146443, Blast_Score=128, Evalue=8e-31,
Organism=Escherichia coli, GI1790860, Length=146, Percent_Identity=32.8767123287671, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1789809, Length=123, Percent_Identity=30.0813008130081, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786911, Length=136, Percent_Identity=30.8823529411765, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787487, Length=100, Percent_Identity=33, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1789402, Length=111, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI145693157, Length=121, Percent_Identity=31.404958677686, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1787375, Length=108, Percent_Identity=31.4814814814815, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 52241; Mature: 52241

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALASKTPDVLLSDIRMPGM
CCCCEEEEEECCCHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHCCCHHHHHHHCCCCCC
DGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYLPKPFDIDEAVALVERAIS
CHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHH
HYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRSSISVLINGESGTGKELVAHA
HHHHHCCCCCEEECCCCHHHCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHH
LHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFTGANTIRQGRFEQADGGTLFLDE
HHHCCCCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCEEEHHH
IGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRIIAATHQNLELRVQEGKFREDLFHR
HCCCCCHHHHHHHHHHHCCCEEEECCCCEEEEEEEEEEEECCCCEEEEECCCHHHHHHHH
LNVIRVHLPPLRERREDIPRLARHFLQVAARELGVEAKLLHPETEAALTRLAWPGNVRQL
HCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHCCCCCHHHH
ENTCRWLTVMAAGQEVLIQDLPGELFESTVAESTSQMQPDSWATLLAQWADRALRSGHQN
HHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LLSEAQPELERTLLTTALRHTQGHKQEAARLLGWGRNTLTRKLKELGME
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALASKTPDVLLSDIRMPGM
CCCCEEEEEECCCHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHCCCHHHHHHHCCCCCC
DGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYLPKPFDIDEAVALVERAIS
CHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHH
HYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRSSISVLINGESGTGKELVAHA
HHHHHCCCCCEEECCCCHHHCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHH
LHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFTGANTIRQGRFEQADGGTLFLDE
HHHCCCCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCEEEHHH
IGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRIIAATHQNLELRVQEGKFREDLFHR
HCCCCCHHHHHHHHHHHCCCEEEECCCCEEEEEEEEEEEECCCCEEEEECCCHHHHHHHH
LNVIRVHLPPLRERREDIPRLARHFLQVAARELGVEAKLLHPETEAALTRLAWPGNVRQL
HCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHCCCCCHHHH
ENTCRWLTVMAAGQEVLIQDLPGELFESTVAESTSQMQPDSWATLLAQWADRALRSGHQN
HHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LLSEAQPELERTLLTTALRHTQGHKQEAARLLGWGRNTLTRKLKELGME
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]