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The map label for this gene is glnL

Identifier: 218701432

GI number: 218701432

Start: 3263960

End: 3265009

Strand: Direct

Name: glnL

Synonym: ECIAI39_3132

Alternate gene names: 218701432

Gene position: 3263960-3265009 (Clockwise)

Preceding gene: 218701431

Following gene: 218701433

Centisome position: 63.6

GC content: 55.52

Gene sequence:

>1050_bases
ATGGCAACAGGCACGCAGCCCGATGCTGGGCAGATCCTCAACTCGCTGATTAACAGTATTTTGTTAATCGATGACAACCT
GGCGATCCATTACGCCAACCCCGCCGCGCAACAACTGCTCGCCCAAAGCTCCCGCAAATTGTTCGGAACACCGTTACCGG
AACTGTTGAGCTACTTCTCATTAAATATCGAGCTGATGCAAGAAAGTCTGGAGGCGGGGCAAGGTTTTACCGATAACGAA
GTGACGCTGGTCATCGACGGGCGCTCGCATATCCTTTCTGTGACGGCCCAGCGTATGCCGGACGGCATGATCCTGCTGGA
GATGGCACCGATGGATAACCAGCGCCGCTTAAGTCAGGAACAGCTACAGCACGCCCAGCAGGTTGCTGCCCGTGATTTAG
TGCGCGGCCTGGCACATGAGATTAAAAATCCGCTTGGCGGTTTACGTGGCGCGGCGCAGCTGCTCAGCAAAGCATTACCT
GACCCGTCACTACTCGAATATACCAAAGTGATTATCGAACAGGCGGACCGGCTGCGAAATCTGGTCGACCGTCTGTTGGG
GCCGCAGCTGCCCGGTACGCGTATTACCGAAAGTATTCACAAAGTGGCTGAACGCGTGGTGACGCTGGTGTCGATGGAAC
TGCCGAGCAACGTGCGGTTGATTCGGGATTACGACCCCAGCCTGCCGGAACTGGCGCACGACCCGGATCAAATTGAACAG
GTCTTGCTGAATATTGTGCGCAATGCGCTACAGGCGCTGGGGCCGGAGGGTGGTGAAATCATTCTGCGTACCCGCACCGC
GTTTCAACTGACCTTACACGGCGAGCGCTATCGGCTGGCGGCGCGGATTGATGTGGAAGATAACGGGCCGGGCATTCCGC
CTCATTTGCAGGATACGCTGTTTTACCCGATGGTCAGCGGCCGCGAAGGTGGCACCGGGCTTGGCTTATCCATCGCCCGT
AATTTGATTGATCAGCATTCAGGCAAAATTGAATTTACCAGTTGGCCAGGTCATACCGAGTTCTCGGTTTACCTGCCTAT
CAGGAAATAA

Upstream 100 bases:

>100_bases
ATGGGCTTTTTTCTCCGTCAATTCTCTGATGCTTCGCGCTTTTTATCCGTAAAAAGCTATAATGCACTAAAATGGTGCAA
CCTGTTCAGGAGACTGCTTT

Downstream 100 bases:

>100_bases
AGGTGACGTTTATGCAACGAGGGATAGTCTGGGTAGTCGATGACGATAGTTCCATCCGTTGGGTGCTTGAACGTGCGCTC
GCTGGAGCGGGTTTAACCTG

Product: nitrogen regulation protein NR(II)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 349; Mature: 348

Protein sequence:

>349_residues
MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSSRKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNE
VTLVIDGRSHILSVTAQRMPDGMILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRITESIHKVAERVVTLVSMELPSNVRLIRDYDPSLPELAHDPDQIEQ
VLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGERYRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIAR
NLIDQHSGKIEFTSWPGHTEFSVYLPIRK

Sequences:

>Translated_349_residues
MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSSRKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNE
VTLVIDGRSHILSVTAQRMPDGMILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRITESIHKVAERVVTLVSMELPSNVRLIRDYDPSLPELAHDPDQIEQ
VLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGERYRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIAR
NLIDQHSGKIEFTSWPGHTEFSVYLPIRK
>Mature_348_residues
ATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSSRKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEV
TLVIDGRSHILSVTAQRMPDGMILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALPD
PSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRITESIHKVAERVVTLVSMELPSNVRLIRDYDPSLPELAHDPDQIEQV
LLNIVRNALQALGPEGGEIILRTRTAFQLTLHGERYRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARN
LIDQHSGKIEFTSWPGHTEFSVYLPIRK

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: COG3852

COG function: function code T; Signal transduction histidine kinase, nitrogen specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=349, Percent_Identity=99.4269340974212, Blast_Score=698, Evalue=0.0,
Organism=Escherichia coli, GI1788549, Length=351, Percent_Identity=28.2051282051282, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI1790436, Length=239, Percent_Identity=30.5439330543933, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI87081816, Length=251, Percent_Identity=30.2788844621514, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI87082128, Length=232, Percent_Identity=31.4655172413793, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1789149, Length=242, Percent_Identity=27.6859504132231, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1786600, Length=351, Percent_Identity=25.9259259259259, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1790567, Length=346, Percent_Identity=26.878612716763, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786912, Length=258, Percent_Identity=28.2945736434109, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1790346, Length=250, Percent_Identity=26.4, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 38543; Mature: 38412

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS50112 PAS ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSSRKLFGTPLPELLSYFS
CCCCCCCCHHHHHHHHHHHEEEEECCEEEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHH
LNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDGMILLEMAPMDNQRRLSQE
HHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHH
QLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALPDPSLLEYTKVIIEQADRLRN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
LVDRLLGPQLPGTRITESIHKVAERVVTLVSMELPSNVRLIRDYDPSLPELAHDPDQIEQ
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHCCCHHHHHH
VLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGERYRLAARIDVEDNGPGIPPHLQDTL
HHHHHHHHHHHHHCCCCCEEEEEEEEEEEEEECCCEEEEEEEEEECCCCCCCCCCCHHHH
FYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTSWPGHTEFSVYLPIRK
HHCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEECCC
>Mature Secondary Structure 
ATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSSRKLFGTPLPELLSYFS
CCCCCCCHHHHHHHHHHHEEEEECCEEEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHH
LNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDGMILLEMAPMDNQRRLSQE
HHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHH
QLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALPDPSLLEYTKVIIEQADRLRN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
LVDRLLGPQLPGTRITESIHKVAERVVTLVSMELPSNVRLIRDYDPSLPELAHDPDQIEQ
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHCCCHHHHHH
VLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGERYRLAARIDVEDNGPGIPPHLQDTL
HHHHHHHHHHHHHCCCCCEEEEEEEEEEEEEECCCEEEEEEEEEECCCCCCCCCCCHHHH
FYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTSWPGHTEFSVYLPIRK
HHCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]