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The map label for this gene is yifB

Identifier: 218701320

GI number: 218701320

Start: 3131067

End: 3132617

Strand: Direct

Name: yifB

Synonym: ECIAI39_3019

Alternate gene names: 218701320

Gene position: 3131067-3132617 (Clockwise)

Preceding gene: 218701313

Following gene: 218701322

Centisome position: 61.01

GC content: 50.81

Gene sequence:

>1551_bases
ATGCACATAATGGTGACTCAAGGAGGGCTTATGTCACTGTCAATTGTTCATACCCGCGCAGCCCTGGGAGTAAATGCGCC
CCCAATCACTGTTGAGGTACATATCAGTAAAGGTCTACCCGGCTTAACGATGGTGGGCTTACCAGAAACAACGGTAAAAG
AAGCCCGCGATCGCGTGCGCAGCGCCATTATCAATAGCGGATATGAATATCCGGCGAAAAAAATCACCATCAATCTTGCT
CCAGCCGATCTGCCAAAAGAAGGGGGACGATATGATTTACCTATCGCTATTGTGTTGCTGGCGGCCTCAGAACAGCTTAC
AGCCAATAAGTTAGATGAATATGAATTAGTCGGAGAACTGGCGCTTACAGGCGCTCTGCGTGGCGTTCCCGGCGCAATTT
CCAGTGCAACTGAAGCTATTAAGGCAGGTAGAAAAATTATCGTCGCGAAAGATAACGAAGATGAAGTGGGGCTAATTAAC
GGTGAAGGATGCCTGATAGCCGATCATCTGCAGGCTGTCTGTGCGTTTCTGGAGGGTAAGCACGCTCTCGAACGCCCGAA
ACCAACTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGTGATGTTGTCGGTCAGGAGCAAGGAAAGCGAGGACTGG
AAATTACCGCCGCTGGCGGGCACAACCTTTTACTGATTGGGCCGCCGGGAACAGGTAAAACAATGCTCGCCAGCCGTATT
AATGGCCTGTTGCCAGATTTAAGCAATGAAGAGGCACTGGAGAGCGCTGCGATATTAAGCCTGGTAAATGCTGAATCAGT
ACAAAAACAATGGCGGCAGCGCCCGTTCCGCTCACCGCATCACAGTGCGTCGTTAACTGCGATGGTGGGCGGTGGTGCAA
TTCCAGGGCCAGGTGAAATTTCGCTGGCGCATAACGGCGTGCTTTTTCTTGATGAGCTACCTGAATTTGAACGGCGTACA
CTGGATGCCTTGCGAGAGCCGATTGAATCCGGGCAGATCCATCTTTCACGCACACGAGCAAAAATAACCTATCCAGCCCG
TTTCCAACTTGTTGCGGCGATGAATCCCAGCCCTACCGGACATTATCAGGGAAACCATAACCGCTGCACACCAGAGCAAA
CGCTGCGTTATCTCAACCGACTCTCTGGTCCCTTTCTCGACCGCTTCGATCTCTCACTGGAGATCCCATTACCGCCACCC
GGCATTTTGAGTAAAACGGTAGTGCCGGGAGAAAACAGCACCACCGTTAAACAACGTGTAATGGCCGCCAGAGAGCGCCA
ATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCAGAAATACGCCAATTCTGCAAGCTTGAGAGCGAAGATG
CGCAGTGGCTGGAAGAAACGCTGATCCATCTGGGGTTATCGATTCGTGCCTGGCAGCGGTTATTGAAAGTTGCACGAACC
ATTGCTGATATTGATCAGTCCGACATTATCACACGTCAGCATTTGCAGGAGGCAGTTAGCTATCGTGCCATTGACCGTTT
GCTCATCCATCTGCAAAAACTACTGACATAA

Upstream 100 bases:

>100_bases
ACATCATTCCACCGCCAGAACAACTCAACTTCACTTGCATAAATAAACAGGAATCAGCCACTAAAATTCACGAATTAGCG
GGCTACAGCAGCGATATCTT

Downstream 100 bases:

>100_bases
AAAAAGGGCATTTCGCCCTTTTTATTAATCGTCAGAATCGGTGTAGTCTTCAGCACCTTCAACCTGCGGTTTACCGCCGG
AAAGGGTGTGAAAACGTTTT

Product: putative ATP-dependent protease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 516; Mature: 516

Protein sequence:

>516_residues
MHIMVTQGGLMSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKEARDRVRSAIINSGYEYPAKKITINLA
PADLPKEGGRYDLPIAIVLLAASEQLTANKLDEYELVGELALTGALRGVPGAISSATEAIKAGRKIIVAKDNEDEVGLIN
GEGCLIADHLQAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGPPGTGKTMLASRI
NGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLTAMVGGGAIPGPGEISLAHNGVLFLDELPEFERRT
LDALREPIESGQIHLSRTRAKITYPARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPP
GILSKTVVPGENSTTVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAQWLEETLIHLGLSIRAWQRLLKVART
IADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT

Sequences:

>Translated_516_residues
MHIMVTQGGLMSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKEARDRVRSAIINSGYEYPAKKITINLA
PADLPKEGGRYDLPIAIVLLAASEQLTANKLDEYELVGELALTGALRGVPGAISSATEAIKAGRKIIVAKDNEDEVGLIN
GEGCLIADHLQAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGPPGTGKTMLASRI
NGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLTAMVGGGAIPGPGEISLAHNGVLFLDELPEFERRT
LDALREPIESGQIHLSRTRAKITYPARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPP
GILSKTVVPGENSTTVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAQWLEETLIHLGLSIRAWQRLLKVART
IADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT
>Mature_516_residues
MHIMVTQGGLMSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKEARDRVRSAIINSGYEYPAKKITINLA
PADLPKEGGRYDLPIAIVLLAASEQLTANKLDEYELVGELALTGALRGVPGAISSATEAIKAGRKIIVAKDNEDEVGLIN
GEGCLIADHLQAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGPPGTGKTMLASRI
NGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLTAMVGGGAIPGPGEISLAHNGVLFLDELPEFERRT
LDALREPIESGQIHLSRTRAKITYPARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPP
GILSKTVVPGENSTTVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAQWLEETLIHLGLSIRAWQRLLKVART
IADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT

Specific function: Unknown

COG id: COG0606

COG function: function code O; Predicted ATPase with chaperone activity

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Mg-chelatase subunits D/I family. ComM subfamily [H]

Homologues:

Organism=Escherichia coli, GI145693202, Length=506, Percent_Identity=98.6166007905138, Blast_Score=1015, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR004482
- InterPro:   IPR000523
- InterPro:   IPR020568 [H]

Pfam domain/function: PF01078 Mg_chelatase [H]

EC number: NA

Molecular weight: Translated: 56340; Mature: 56340

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIMVTQGGLMSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKEARDRVR
CEEEEECCCCEEEEEHHHHHHCCCCCCCEEEEEEECCCCCCCEEECCCCHHHHHHHHHHH
SAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIVLLAASEQLTANKLDEYELVGEL
HHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCHHHHHCCHHHHHHHHH
ALTGALRGVPGAISSATEAIKAGRKIIVAKDNEDEVGLINGEGCLIADHLQAVCAFLEGK
HHHHHHCCCCCHHHHHHHHHHCCCEEEEEECCCCCEEEECCCCCEEHHHHHHHHHHHHCH
HALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGPPGTGKTMLASRI
HHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEECCCCCCHHHHHHHH
NGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLTAMVGGGAIPGPGEI
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCE
SLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITYPARFQLVAAMNPSPTG
EEECCCEEEECCCCHHHHHHHHHHHHHHHCCCEEEEECEEEEECCCEEEEEEECCCCCCC
HYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGILSKTVVPGENSTTVKQRV
CCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHH
MAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAQWLEETLIHLGLSIRAWQRLLKVART
HHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
IADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MHIMVTQGGLMSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKEARDRVR
CEEEEECCCCEEEEEHHHHHHCCCCCCCEEEEEEECCCCCCCEEECCCCHHHHHHHHHHH
SAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIVLLAASEQLTANKLDEYELVGEL
HHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCHHHHHCCHHHHHHHHH
ALTGALRGVPGAISSATEAIKAGRKIIVAKDNEDEVGLINGEGCLIADHLQAVCAFLEGK
HHHHHHCCCCCHHHHHHHHHHCCCEEEEEECCCCCEEEECCCCCEEHHHHHHHHHHHHCH
HALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGPPGTGKTMLASRI
HHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEECCCCCCHHHHHHHH
NGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLTAMVGGGAIPGPGEI
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCE
SLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITYPARFQLVAAMNPSPTG
EEECCCEEEECCCCHHHHHHHHHHHHHHHCCCEEEEECEEEEECCCEEEEEEECCCCCCC
HYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGILSKTVVPGENSTTVKQRV
CCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHH
MAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAQWLEETLIHLGLSIRAWQRLLKVART
HHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
IADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1995430; 1379743; 9278503 [H]