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The map label for this gene is nrdF

Identifier: 218701171

GI number: 218701171

Start: 2972907

End: 2973866

Strand: Direct

Name: nrdF

Synonym: ECIAI39_2866

Alternate gene names: 218701171

Gene position: 2972907-2973866 (Clockwise)

Preceding gene: 218701170

Following gene: 218701172

Centisome position: 57.93

GC content: 48.85

Gene sequence:

>960_bases
ATGAAACTCTCACGTATCAGCGCCATCAACTGGAACAAGATATCTGACGATAAAGATCTGGAGGTGTGGAATCGCCTGAC
CAGCAATTTCTGGCTACCAGAAAAGGTGCCGCTGTCGAACGATATTCCTGCATGGCAGACATTAAGTGTCGTAGAACAAC
AACTGACGATGCGCGTTTTTACTGGCCTGACGCTGCTCGACACGCTGCAAAATGTTATCGGCGCGCCTTCGCTGATGCCC
GATGCGCTCACGCCCCATGAAGAGGCGGTATTAACGAATATCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTC
GATTTTCTCGACGCTGTGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCGAA
AAGCTCAGATTATTCAGCAACATTATCGCGGGGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTT
TTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTAACCAATACCGCAGACCTGATCCGTCT
GATTATCCGGGATGAAGCAGTCCACGGTTACTACATCGGCTATAAATATCAGAAAAACCTGGAAAAGATCTCCCTGGCAC
AACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTATTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTG
TACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCGTTGATGAATCTGGGCTA
CGAACCGTTATTTCCCGCAGAAATGGCAGAAGTGAATCCGGCAATCCTCGCCGCGCTTTCGCCGAATGCCGATGAAAATC
ACGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATGGGAAAAGCGGTCGAAACAGAAGATGAAGACTGGAATTTCTGA

Upstream 100 bases:

>100_bases
CAAAGGTATCAAAACACTCTATTACATTCGCCTGCGTCAGATGGCGCTGGAAGGCACTGAAATTGAAGGCTGCGTCTCCT
GTGCACTTTAAGGAATATCT

Downstream 100 bases:

>100_bases
GGGGGTTATTTTCAAAAATATCACTACCCGCGACAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATATTTT
GTGGTGATCTACACTGACAC

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: R2F protein; Ribonucleotide reductase 2 [H]

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPAWQTLSVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMP
DALTPHEEAVLTNISFMEAVHARSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNLEKISLAQREELKSFAFDLLLELYDNELQYTDEL
YAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMAEVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF

Sequences:

>Translated_319_residues
MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPAWQTLSVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMP
DALTPHEEAVLTNISFMEAVHARSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNLEKISLAQREELKSFAFDLLLELYDNELQYTDEL
YAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMAEVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF
>Mature_319_residues
MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPAWQTLSVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMP
DALTPHEEAVLTNISFMEAVHARSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNLEKISLAQREELKSFAFDLLLELYDNELQYTDEL
YAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMAEVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. R2F contains the tyrosyl radical required for catalysis [H]

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family [H]

Homologues:

Organism=Escherichia coli, GI1789031, Length=319, Percent_Identity=98.7460815047022, Blast_Score=656, Evalue=0.0,
Organism=Escherichia coli, GI1788567, Length=267, Percent_Identity=25.8426966292135, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358 [H]

Pfam domain/function: PF00268 Ribonuc_red_sm [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 36439; Mature: 36439

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: PS00368 RIBORED_SMALL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPAWQTLSVVEQQLTMRVF
CCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
TGLTLLDTLQNVIGAPSLMPDALTPHEEAVLTNISFMEAVHARSYSSIFSTLCQTKDVDA
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
AYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESFLFYSGFWLPMYFSSRGKLTN
HEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHEEEECCCCCCCH
TADLIRLIIRDEAVHGYYIGYKYQKNLEKISLAQREELKSFAFDLLLELYDNELQYTDEL
HHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
YAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMAEVNPAILAALSPNADENHDFFSGS
HHCCCCHHHHHHHEEECCCHHHHHCCCCCCCCHHHHHCCHHEEEEECCCCCCCCCCCCCC
GSSYVMGKAVETEDEDWNF
CCCEEEECEECCCCCCCCC
>Mature Secondary Structure
MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPAWQTLSVVEQQLTMRVF
CCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
TGLTLLDTLQNVIGAPSLMPDALTPHEEAVLTNISFMEAVHARSYSSIFSTLCQTKDVDA
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
AYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESFLFYSGFWLPMYFSSRGKLTN
HEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHEEEECCCCCCCH
TADLIRLIIRDEAVHGYYIGYKYQKNLEKISLAQREELKSFAFDLLLELYDNELQYTDEL
HHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
YAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMAEVNPAILAALSPNADENHDFFSGS
HHCCCCHHHHHHHEEECCCHHHHHCCCCCCCCHHHHHCCHHEEEEECCCCCCCCCCCCCC
GSSYVMGKAVETEDEDWNF
CCCEEEECEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 2649479; 8195103 [H]