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The map label for this gene is nrdE

Identifier: 218701170

GI number: 218701170

Start: 2970753

End: 2972897

Strand: Direct

Name: nrdE

Synonym: ECIAI39_2865

Alternate gene names: 218701170

Gene position: 2970753-2972897 (Clockwise)

Preceding gene: 218701169

Following gene: 218701171

Centisome position: 57.89

GC content: 51.98

Gene sequence:

>2145_bases
TTGGCAACGACAACCGCAGAACGCCTGACGCAGGAAACGATGGATTATCACGCTCTGAATGCGATGCTTAACCTTTACGA
TAGCGCAGGTCGCATTCAGTTCGATAAAGATCGCCAGGCAGTAGACGCCTTTATGACGACGCATGTGCGTCCGAACAGTG
TGGCCTTCAGTAGCCAGCAGCAGCGCCTGAACTGGCTGGTTAACGAAGGTTACTATGATGAAAGTGTCCTTAATCGCTAC
TCTCGCGATTTTGTCATTACGCTGTTTGCCCACGCGCACGCCAGCGGTTTTCGTTTCCAGACATTCCTCGGAGCATGGAA
GTTTTACACCAGCTATACGTTGAAGACATTCGACGGTAAGCGTTATCTGGAAGATTTTGCCGATCGCGTCACGATGGTGG
CGCTGACGCTGGCACAAGGCGATGAAACACTGGCGACGCAGCTCACTGATGAGATGCTGTCAGGACGCTTTCAGCCAGCC
ACGCCAACATTCCTCAACTGCGGTAAGCAGCAGCGCGGCGAACTGGTTTCCTGTTTTCTGCTGCGTATTGAAGACAATAT
GGAGTCGATTGGCCGGGCAGTAAATTCCGCACTACAACTGTCGAAACGCGGCGGCGGCGTAGCATTTTTGCTGTCGAATC
TGCGGGAAGCGGGCGCGCCAATTAAACGTATTGAAAATCAATCTTCTGGCGTGATTCCGGTGATGAAAATGCTGGAAGAC
GCATTTTCCTATGCCAACCAACTCGGCGCGCGTCAGGGGGCTGGGGCAGTCTATTTACATGCTCATCATCCCGATATTCT
GCGTTTTCTCGATACAAAACGGGAAAATGCCGACGAAAAAATCCGCATTAAAACACTGTCGCTGGGCGTGGTGATCCCGG
ATATCACTTTCCATCTGGCGAAAGAGAATGCGCAGATGGCGCTGTTTTCGCCCTATGACGTTGAGCGGGTTTATGGCAAG
CCGTTTGCCGATGTCGCCATCAGTGAACACTATGACGAACTGGTTGCCGATGAACGCATTCGCAAAAAATACCTCAACGC
CCGTGATTTCTTCCAGCGACTGGCAGAGATCCAGTTTGAGTCTGGCTATCCGTACATCATGTATGAAGACACGGTTAACC
GTGCTAACCCTATCGCCGGGCGCATAAATATGAGCAATCTCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAATAT
GACGAGAATCTCGACTATGCTCGTACAGGCCATGATATTTCCTGCAATCTAGGGTCGTTGAATATTGCCCACACCATGGA
TTCCCCCGACTTTGCCCGCACGGTAGAGACTGCCGTACGCGGTTTAACGGCGGTGTCAGATATGAGTCATATCCGCAGCG
TGCCGTCCATCGAAGCCGGAAATGCCGCCTCACACGCCATCGGTCTGGGGCAGATGAATTTACACGGTTATCTGGCGCGA
GAAGGCATCGCTTATGGTTCGCCGGAAGCACTGGATTTCACCAATCTCTATTTCTATACCATCACCTGGCACGCACTGCG
TACCTCGATGTTGCTGGCGCGCGAACGCGGTGAAACCTTCGCCGGGTTCAAACAGTCACGCTATGCCAGTGGTGAATATT
TCAGCCAATATCTGCAAAGTAACTGGCAGCCGAAAACGGCGAAAGTTGGCGAACTGTTTGCCCGTAGTGGTATTACGTTA
CCTACCCGTGAGATGTGGGCGCAGCTGCGCGACGACGTGATGCGCTATGGCATATACAACCAGAATCTCCAGGCGGTGCC
GCCAACCGGTTCTATCTCTTATATCAACCATGCCACGTCGAGTATTCATCCGATTGTGGCGAAAGTAGAGATACGCAAAG
AGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTATGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAA
ATCGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCGACTCGCCATGTCGATCAGGGGCTGTCGCTGACGCTGTTTTT
CCACGATACCGCCACCACTCGCGATATCAACAAAGCGCAGATTTACGCCTGGCGCAAAGGTATCAAAACACTCTATTACA
TTCGCCTGCGTCAGATGGCGCTGGAAGGCACTGAAATTGAAGGCTGCGTCTCCTGTGCACTTTAA

Upstream 100 bases:

>100_bases
ATGTGATTGCCCGGAAATGCGGCGTGCCGTGGCTGTACCGCTTTGAACTCATGGGTACGCAAAGCGATATCGAAAACGTT
CGTAAAGGAGTAACCGAATT

Downstream 100 bases:

>100_bases
GGAATATCTATGAAACTCTCACGTATCAGCGCCATCAACTGGAACAAGATATCTGACGATAAAGATCTGGAGGTGTGGAA
TCGCCTGACCAGCAATTTCT

Product: ribonucleotide-diphosphate reductase subunit alpha

Products: NA

Alternate protein names: R1E protein; Ribonucleotide reductase 2 [H]

Number of amino acids: Translated: 714; Mature: 713

Protein sequence:

>714_residues
MATTTAERLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAFMTTHVRPNSVAFSSQQQRLNWLVNEGYYDESVLNRY
SRDFVITLFAHAHASGFRFQTFLGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLATQLTDEMLSGRFQPA
TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAGAPIKRIENQSSGVIPVMKMLED
AFSYANQLGARQGAGAVYLHAHHPDILRFLDTKRENADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGK
PFADVAISEHYDELVADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEILQVNSASEY
DENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSHIRSVPSIEAGNAASHAIGLGQMNLHGYLAR
EGIAYGSPEALDFTNLYFYTITWHALRTSMLLARERGETFAGFKQSRYASGEYFSQYLQSNWQPKTAKVGELFARSGITL
PTREMWAQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPAPFMTNENLALYQDAYE
IGAEKIIDTYAEATRHVDQGLSLTLFFHDTATTRDINKAQIYAWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL

Sequences:

>Translated_714_residues
MATTTAERLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAFMTTHVRPNSVAFSSQQQRLNWLVNEGYYDESVLNRY
SRDFVITLFAHAHASGFRFQTFLGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLATQLTDEMLSGRFQPA
TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAGAPIKRIENQSSGVIPVMKMLED
AFSYANQLGARQGAGAVYLHAHHPDILRFLDTKRENADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGK
PFADVAISEHYDELVADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEILQVNSASEY
DENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSHIRSVPSIEAGNAASHAIGLGQMNLHGYLAR
EGIAYGSPEALDFTNLYFYTITWHALRTSMLLARERGETFAGFKQSRYASGEYFSQYLQSNWQPKTAKVGELFARSGITL
PTREMWAQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPAPFMTNENLALYQDAYE
IGAEKIIDTYAEATRHVDQGLSLTLFFHDTATTRDINKAQIYAWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL
>Mature_713_residues
ATTTAERLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAFMTTHVRPNSVAFSSQQQRLNWLVNEGYYDESVLNRYS
RDFVITLFAHAHASGFRFQTFLGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLATQLTDEMLSGRFQPAT
PTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAGAPIKRIENQSSGVIPVMKMLEDA
FSYANQLGARQGAGAVYLHAHHPDILRFLDTKRENADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKP
FADVAISEHYDELVADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEILQVNSASEYD
ENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSHIRSVPSIEAGNAASHAIGLGQMNLHGYLARE
GIAYGSPEALDFTNLYFYTITWHALRTSMLLARERGETFAGFKQSRYASGEYFSQYLQSNWQPKTAKVGELFARSGITLP
TREMWAQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPAPFMTNENLALYQDAYEI
GAEKIIDTYAEATRHVDQGLSLTLFFHDTATTRDINKAQIYAWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. R1E contains the binding sites for both substrates and allosteric effectors and carries out the actual reductio

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase large chain family [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=601, Percent_Identity=27.9534109816972, Blast_Score=185, Evalue=2e-46,
Organism=Escherichia coli, GI1789030, Length=714, Percent_Identity=98.1792717086835, Blast_Score=1465, Evalue=0.0,
Organism=Escherichia coli, GI2367133, Length=582, Percent_Identity=25.4295532646048, Blast_Score=149, Evalue=7e-37,
Organism=Caenorhabditis elegans, GI17555242, Length=749, Percent_Identity=25.2336448598131, Blast_Score=180, Evalue=3e-45,
Organism=Saccharomyces cerevisiae, GI6320914, Length=619, Percent_Identity=26.1712439418417, Blast_Score=170, Evalue=7e-43,
Organism=Saccharomyces cerevisiae, GI6322123, Length=643, Percent_Identity=25.8164852255054, Blast_Score=169, Evalue=1e-42,
Organism=Drosophila melanogaster, GI17352481, Length=672, Percent_Identity=24.2559523809524, Blast_Score=150, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926
- InterPro:   IPR013554 [H]

Pfam domain/function: PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN; PF08343 RNR_N [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 80520; Mature: 80389

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: PS00089 RIBORED_LARGE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATTTAERLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAFMTTHVRPNSVAFSSQQ
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHCCCCCCCCCCCCHH
QRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHASGFRFQTFLGAWKFYTSYTLKTFDGK
HHHHHHHCCCCCCHHHHHHHCCCEEEEEEEHHCCCCCCHHHHHHHHHHHHEEEEEECCCH
RYLEDFADRVTMVALTLAQGDETLATQLTDEMLSGRFQPATPTFLNCGKQQRGELVSCFL
HHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHCCCHHHCCHHHHHH
LRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAGAPIKRIENQSSGVIPVMKMLED
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHH
AFSYANQLGARQGAGAVYLHAHHPDILRFLDTKRENADEKIRIKTLSLGVVIPDITFHLA
HHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHCCCCCCEEEEEEEEEEEEECCCEEEEE
KENAQMALFSPYDVERVYGKPFADVAISEHYDELVADERIRKKYLNARDFFQRLAEIQFE
CCCCCEEEECCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCEEC
SGYPYIMYEDTVNRANPIAGRINMSNLCSEILQVNSASEYDENLDYARTGHDISCNLGSL
CCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHCCCCHHHCCCCEEECCCCE
NIAHTMDSPDFARTVETAVRGLTAVSDMSHIRSVPSIEAGNAASHAIGLGQMNLHGYLAR
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHEECCCCCCCHHHHH
EGIAYGSPEALDFTNLYFYTITWHALRTSMLLARERGETFAGFKQSRYASGEYFSQYLQS
CCCCCCCCCCEEECEEEEEEEEHHHHHHHHHHHHHCCCHHHCHHHHCCCCHHHHHHHHHC
NWQPKTAKVGELFARSGITLPTREMWAQLRDDVMRYGIYNQNLQAVPPTGSISYINHATS
CCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCHHHHHCCC
SIHPIVAKVEIRKEGKTGRVYYPAPFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQG
CCCCEEEEEEEEECCCCCEEEECCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCC
LSLTLFFHDTATTRDINKAQIYAWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL
CEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCC
>Mature Secondary Structure 
ATTTAERLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAFMTTHVRPNSVAFSSQQ
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHCCCCCCCCCCCCHH
QRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHASGFRFQTFLGAWKFYTSYTLKTFDGK
HHHHHHHCCCCCCHHHHHHHCCCEEEEEEEHHCCCCCCHHHHHHHHHHHHEEEEEECCCH
RYLEDFADRVTMVALTLAQGDETLATQLTDEMLSGRFQPATPTFLNCGKQQRGELVSCFL
HHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHCCCHHHCCHHHHHH
LRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAGAPIKRIENQSSGVIPVMKMLED
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHH
AFSYANQLGARQGAGAVYLHAHHPDILRFLDTKRENADEKIRIKTLSLGVVIPDITFHLA
HHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHCCCCCCEEEEEEEEEEEEECCCEEEEE
KENAQMALFSPYDVERVYGKPFADVAISEHYDELVADERIRKKYLNARDFFQRLAEIQFE
CCCCCEEEECCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCEEC
SGYPYIMYEDTVNRANPIAGRINMSNLCSEILQVNSASEYDENLDYARTGHDISCNLGSL
CCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHCCCCHHHCCCCEEECCCCE
NIAHTMDSPDFARTVETAVRGLTAVSDMSHIRSVPSIEAGNAASHAIGLGQMNLHGYLAR
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHEECCCCCCCHHHHH
EGIAYGSPEALDFTNLYFYTITWHALRTSMLLARERGETFAGFKQSRYASGEYFSQYLQS
CCCCCCCCCCEEECEEEEEEEEHHHHHHHHHHHHHCCCHHHCHHHHCCCCHHHHHHHHHC
NWQPKTAKVGELFARSGITLPTREMWAQLRDDVMRYGIYNQNLQAVPPTGSISYINHATS
CCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCHHHHHCCC
SIHPIVAKVEIRKEGKTGRVYYPAPFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQG
CCCCEEEEEEEEECCCCCEEEECCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCC
LSLTLFFHDTATTRDINKAQIYAWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL
CEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 8820648; 2158980 [H]