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The map label for this gene is csiR

Identifier: 218701157

GI number: 218701157

Start: 2963210

End: 2963872

Strand: Direct

Name: csiR

Synonym: ECIAI39_2852

Alternate gene names: 218701157

Gene position: 2963210-2963872 (Clockwise)

Preceding gene: 218701156

Following gene: 218701160

Centisome position: 57.74

GC content: 52.94

Gene sequence:

>663_bases
ATGACCATTACGTCTCTGGATGGCTATCGCTGGCTGAAGAACGATATTATTCGCGGTAATTTTCAACCGGATGAAAAATT
ACGGATGAGTTTGCTGACATCGCGTTATGCACTTGGCGTTGGGCCGTTACGGGAAGCTCTTTCGCAACTGGTGGCGGAAC
GGCTGGTCACGGTGGTGAATCAAAAAGGGTATCGGGTGGCGTCTATGTCAGAGCAGGAGCTGCTCGATATTTTCGACGCC
CGCGCCAATATGGAAGCGATGCTGGTGAGTCTGGCGATTGCCCGCGGTGGCGATGAATGGGAGGCAGACGTTCTCGCAAA
AGCGCATCTGCTCAGTAAGCTTGAGGCCTGTGACGCCAGTGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGT
TTCATACTGCAATTGTGGCGGGCTGTGGTTCTTACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGCAGCGCGT
TATCGATTTATCTGGCTGCGGCGAACAGTGCTTTCGGTGGAAATGCTGGAAGATAAACACGATCAGCACCAGACCCTGAC
TGCGGCGGTACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCC
AGCAGGCGATGGCTGGCAATTAA

Upstream 100 bases:

>100_bases
TTATCTGTACCGTGCCAATTATGGCTCGCTGGAAAAAGCTGGTATTGTGGCAAAAAACACCCGTTCATAATACGCGCTGA
TAATGATCAGGAGTCACACC

Downstream 100 bases:

>100_bases
CTACTCTTCAGGAATACGCAACACTTGCCCCGGGTAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGA
TTTTATTGTACAGATTAGCG

Product: DNA-binding transcriptional regulator CsiR

Products: NA

Alternate protein names: Carbon starvation induced regulator [H]

Number of amino acids: Translated: 220; Mature: 219

Protein sequence:

>220_residues
MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLREALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDA
RANMEAMLVSLAIARGGDEWEADVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSYYLLQMRERLFDLAAR
YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMAGN

Sequences:

>Translated_220_residues
MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLREALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDA
RANMEAMLVSLAIARGGDEWEADVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSYYLLQMRERLFDLAAR
YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMAGN
>Mature_219_residues
TITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLREALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDAR
ANMEAMLVSLAIARGGDEWEADVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSYYLLQMRERLFDLAARY
RFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMAGN

Specific function: Modulates expression of csiD and lhgO in a temporal manner during entry into stationary phase or during the first few hours of carbon starvation [H]

COG id: COG1802

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87082149, Length=220, Percent_Identity=99.5454545454545, Blast_Score=448, Evalue=1e-127,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 25017; Mature: 24886

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLREALSQLVAERLVTVVN
CCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
QKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEADVLAKAHLLSKLEACDAS
CCCCEECCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHH
EKMLDEWDLRHQAFHTAIVAGCGSYYLLQMRERLFDLAARYRFIWLRRTVLSVEMLEDKH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMAGN
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCC
>Mature Secondary Structure 
TITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLREALSQLVAERLVTVVN
CCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
QKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEADVLAKAHLLSKLEACDAS
CCCCEECCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHH
EKMLDEWDLRHQAFHTAIVAGCGSYYLLQMRERLFDLAARYRFIWLRRTVLSVEMLEDKH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMAGN
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 8297211; 7984428 [H]