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The map label for this gene is gabP

Identifier: 218701156

GI number: 218701156

Start: 2961789

End: 2963189

Strand: Direct

Name: gabP

Synonym: ECIAI39_2851

Alternate gene names: 218701156

Gene position: 2961789-2963189 (Clockwise)

Preceding gene: 218701153

Following gene: 218701157

Centisome position: 57.71

GC content: 51.39

Gene sequence:

>1401_bases
ATGGGGCAATCATCGCAACCACATGAGTTAGGCGGCGGGCTGAAGTCACGCCACGTCACCATGTTGTCTATTGCCGGTGT
TATCGGCGCAAGTCTGTTTGTCGGTTCCAGCGTCGCCATCGCCGAAGCGGGCCCGGCGGTATTACTGGCCTATCTGTTCG
CCGGATTACTGGTGGTTATGATTATGCGGATGTTGGCGGAAATGGCGGTTGCCACGCCCGATACCGGTTCGTTTTCCACC
TATGCCGATAAAGCCATTGGCCGTTGGGCGGGCTATACCATCGGCTGGTTGTACTGGTGGTTTTGGGTACTGGTTATCCC
ACTGGAAGCCAACATCGCCGCCATGATCCTGCACTCGTGGGTTCCAGGCATTCCCATCTGGTTATTTTCCCTGGTCATTA
CCCTCGCCTTGACTGGCAGTAATTTATTAAGCGTTAAAAACTACGGCGAATTTGAGTTCTGGCTGGCGCTGTGCAAAGTC
ATCGCTATCCTGGCCTTTATTTTCCTTGGTGCAGTCGCAATTAGCGGTTTTTACCCTTATGCCGAAGTGAGCGGGATCTC
AAGATTGTGGGATAGCGGCGGCTTTATGCCCAACGGCTTCGGTGCGGTATTAAGCGCGATGTTGATCACCATGTTCTCGT
TTATGGGCGCAGAAATTGTCACTATTGCCGCCGCGGAATCTGACACGCCGGAAAAACATATTGTCCGCGCCACCAACTCG
GTTATCTGGCGTATTTCTATCTTCTATTTGTGCTCTATTTTTGTCGTCGTGGCATTAATACCGTGGAATATGCCGGGGCT
GAAAGCCGTTGGATCTTATCGCTCGGTTCTGGAGTTGCTCAATATTCCCCATGCGAAATTAATCATGGACTGCGTGATAT
TACTTTCCGTAACCAGTTGCCTGAACTCGGCGCTGTATACCGCTTCCAGAATGCTCTACTCCTTAAGCCGTCGCGGTGAT
GCTCCCGCGGTAATGGGTAAAATCAACCGCAGTAAAACCCCGTATGTGGCGGTATTACTCTCCACCGGCGCGGCATTCTT
AACGGTGGTGGTGAACTATTACGCGCCTGCGAAGGTGTTTAAATTTCTGATCGACAGCTCCGGCGCTATCGCCCTGCTGG
TTTATTTAGTCATCGCCGTTTCACAATTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCGAAATTCGCCTGCGGATG
TGGCTTTATCCGTGGCTCACCTGGCTGGTCATCGGCTTTATTACCTTTGTGTTGGTAGTAATGCTATTCCGCCCGGCGCA
ACAGTTAGAAGTGATCTCCACCGGCTTATTAGCGATTGGGATTATCTGTACCGTGCCAATTATGGCTCGCTGGAAAAAGC
TGGTATTGTGGCAAAAAACACCCGTTCATAATACGCGCTGA

Upstream 100 bases:

>100_bases
CGCTCTCCGGCAAAAAGAACCAATAACAATTAAACGCGTGACCCGACGCGGGAAATGTCGGGGCGCTCTCCCAAGTGACA
CACTTTCGAGAGGATTCAGG

Downstream 100 bases:

>100_bases
TAATGATCAGGAGTCACACCATGACCATTACGTCTCTGGATGGCTATCGCTGGCTGAAGAACGATATTATTCGCGGTAAT
TTTCAACCGGATGAAAAATT

Product: gamma-aminobutyrate transporter

Products: Proton [Cytoplasm]; 4-aminobutyrate [Cytoplasm] [C]

Alternate protein names: 4-amino butyrate transport carrier; Gamma-aminobutyrate permease

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAGPAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFST
YADKAIGRWAGYTIGWLYWWFWVLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAEIVTIAAAESDTPEKHIVRATNS
VIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSVLELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGD
APAVMGKINRSKTPYVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAEGSEIRLRM
WLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMARWKKLVLWQKTPVHNTR

Sequences:

>Translated_466_residues
MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAGPAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFST
YADKAIGRWAGYTIGWLYWWFWVLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAEIVTIAAAESDTPEKHIVRATNS
VIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSVLELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGD
APAVMGKINRSKTPYVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAEGSEIRLRM
WLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMARWKKLVLWQKTPVHNTR
>Mature_465_residues
GQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAGPAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTY
ADKAIGRWAGYTIGWLYWWFWVLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKVI
AILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAEIVTIAAAESDTPEKHIVRATNSV
IWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSVLELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDA
PAVMGKINRSKTPYVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAEGSEIRLRMW
LYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMARWKKLVLWQKTPVHNTR

Specific function: Transporter for GABA

COG id: COG1113

COG function: function code E; Gamma-aminobutyrate permease and related permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily. Amino acid transporter (AAT) (TC 2.A.3.1) family

Homologues:

Organism=Escherichia coli, GI1789017, Length=466, Percent_Identity=100, Blast_Score=929, Evalue=0.0,
Organism=Escherichia coli, GI1786302, Length=433, Percent_Identity=36.7205542725173, Blast_Score=279, Evalue=2e-76,
Organism=Escherichia coli, GI1786789, Length=431, Percent_Identity=36.8909512761021, Blast_Score=270, Evalue=2e-73,
Organism=Escherichia coli, GI48994972, Length=464, Percent_Identity=32.9741379310345, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI87081708, Length=438, Percent_Identity=35.3881278538813, Blast_Score=244, Evalue=1e-65,
Organism=Escherichia coli, GI1788480, Length=392, Percent_Identity=34.1836734693878, Blast_Score=238, Evalue=5e-64,
Organism=Escherichia coli, GI87081915, Length=408, Percent_Identity=33.3333333333333, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI1786602, Length=431, Percent_Identity=33.4106728538283, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI1790653, Length=447, Percent_Identity=29.9776286353468, Blast_Score=225, Evalue=5e-60,
Organism=Caenorhabditis elegans, GI17532491, Length=364, Percent_Identity=22.5274725274725, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320772, Length=443, Percent_Identity=30.4740406320542, Blast_Score=207, Evalue=3e-54,
Organism=Saccharomyces cerevisiae, GI6324061, Length=443, Percent_Identity=29.5711060948081, Blast_Score=199, Evalue=9e-52,
Organism=Saccharomyces cerevisiae, GI6324059, Length=439, Percent_Identity=29.6127562642369, Blast_Score=186, Evalue=8e-48,
Organism=Saccharomyces cerevisiae, GI6324553, Length=389, Percent_Identity=32.133676092545, Blast_Score=181, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6324990, Length=421, Percent_Identity=30.8788598574822, Blast_Score=176, Evalue=7e-45,
Organism=Saccharomyces cerevisiae, GI6324924, Length=464, Percent_Identity=28.6637931034483, Blast_Score=176, Evalue=8e-45,
Organism=Saccharomyces cerevisiae, GI6322892, Length=410, Percent_Identity=30.7317073170732, Blast_Score=169, Evalue=8e-43,
Organism=Saccharomyces cerevisiae, GI6320717, Length=413, Percent_Identity=29.0556900726392, Blast_Score=163, Evalue=6e-41,
Organism=Saccharomyces cerevisiae, GI6319824, Length=408, Percent_Identity=28.4313725490196, Blast_Score=158, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6321053, Length=401, Percent_Identity=27.6807980049875, Blast_Score=157, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6319543, Length=440, Percent_Identity=26.3636363636364, Blast_Score=154, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6321629, Length=400, Percent_Identity=27.5, Blast_Score=150, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6320251, Length=408, Percent_Identity=28.4313725490196, Blast_Score=148, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6319542, Length=404, Percent_Identity=27.7227722772277, Blast_Score=144, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6322967, Length=404, Percent_Identity=26.2376237623762, Blast_Score=141, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6324981, Length=405, Percent_Identity=29.8765432098765, Blast_Score=137, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6319608, Length=437, Percent_Identity=26.0869565217391, Blast_Score=122, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6320364, Length=480, Percent_Identity=23.3333333333333, Blast_Score=84, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24667468, Length=406, Percent_Identity=23.1527093596059, Blast_Score=73, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GABP_ECOLI (P25527)

Other databases:

- EMBL:   M88334
- EMBL:   X65104
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   H65045
- RefSeq:   AP_003236.1
- RefSeq:   NP_417149.1
- ProteinModelPortal:   P25527
- DIP:   DIP-9724N
- MINT:   MINT-1248489
- STRING:   P25527
- EnsemblBacteria:   EBESCT00000003675
- EnsemblBacteria:   EBESCT00000003676
- EnsemblBacteria:   EBESCT00000003677
- EnsemblBacteria:   EBESCT00000014589
- GeneID:   948049
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW2638
- KEGG:   eco:b2663
- EchoBASE:   EB1306
- EcoGene:   EG11330
- eggNOG:   COG1113
- GeneTree:   EBGT00050000009005
- HOGENOM:   HBG492579
- OMA:   LTITNLF
- ProtClustDB:   PRK10197
- BioCyc:   EcoCyc:GABP-MONOMER
- Genevestigator:   P25527
- InterPro:   IPR004841
- InterPro:   IPR002293
- InterPro:   IPR004840
- InterPro:   IPR011265
- PANTHER:   PTHR11785
- PIRSF:   PIRSF006060
- TIGRFAMs:   TIGR01773

Pfam domain/function: PF00324 AA_permease

EC number: NA

Molecular weight: Translated: 51081; Mature: 50950

Theoretical pI: Translated: 9.85; Mature: 9.85

Prosite motif: PS00218 AMINO_ACID_PERMEASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x243c13d8)-; HASH(0x243574c8)-; HASH(0x24453da0)-; HASH(0x2409e16c)-; HASH(0x24408090)-; HASH(0x24189210)-; HASH(0x24453bc0)-; HASH(0x24091678)-; HASH(0x1fa3f414)-; HASH(0x243fd644)-; HASH(0x2434ba04)-; HASH(0x22dfd504)-;

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAGPAVLLAYLFAGLLVVM
CCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHH
IMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFWVLVIPLEANIAAMILHSW
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
VPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKVIAILAFIFLGAVAISGFYPY
CCCHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
AEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAEIVTIAAAESDTPEKHIVRATNS
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHH
VIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSVLELLNIPHAKLIMDCVILLSVTSC
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LNSALYTASRMLYSLSRRGDAPAVMGKINRSKTPYVAVLLSTGAAFLTVVVNYYAPAKVF
HHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCEEEEEHHCCHHHHHHHHHHCCHHHHH
KFLIDSSGAIALLVYLVIAVSQLRMRKILRAEGSEIRLRMWLYPWLTWLVIGFITFVLVV
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHH
MLFRPAQQLEVISTGLLAIGIICTVPIMARWKKLVLWQKTPVHNTR
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
>Mature Secondary Structure 
GQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAGPAVLLAYLFAGLLVVM
CCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHH
IMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFWVLVIPLEANIAAMILHSW
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
VPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKVIAILAFIFLGAVAISGFYPY
CCCHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
AEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAEIVTIAAAESDTPEKHIVRATNS
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHH
VIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSVLELLNIPHAKLIMDCVILLSVTSC
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LNSALYTASRMLYSLSRRGDAPAVMGKINRSKTPYVAVLLSTGAAFLTVVVNYYAPAKVF
HHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCEEEEEHHCCHHHHHHHHHHCCHHHHH
KFLIDSSGAIALLVYLVIAVSQLRMRKILRAEGSEIRLRMWLYPWLTWLVIGFITFVLVV
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHH
MLFRPAQQLEVISTGLLAIGIICTVPIMARWKKLVLWQKTPVHNTR
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 4-aminobutyrate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 4-aminobutyrate [Periplasm] = Proton [Cytoplasm] + 4-aminobutyrate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8297211; 9205837; 9278503